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Aliases for RNF185 Gene

Aliases for RNF185 Gene

  • Ring Finger Protein 185 2 3
  • RING Finger Protein 185 4 5
  • E3 Ubiquitin-Protein Ligase RNF185 3
  • Hypothetical Protein FLJ38628 2
  • BSK65-MONO1 3
  • BSK65-MONO2 3
  • BSK65-PANC1 3
  • BSK65-PANC2 3
  • BSK65-TEST1 3
  • BSK65-TEST2 3
  • BSK65-TEST3 3
  • EC 6.3.2.- 4

External Ids for RNF185 Gene

Previous GeneCards Identifiers for RNF185 Gene

  • GC22P029881
  • GC22P031556
  • GC22P014517

Summaries for RNF185 Gene

GeneCards Summary for RNF185 Gene

RNF185 (Ring Finger Protein 185) is a Protein Coding gene. Diseases associated with RNF185 include Cystic Fibrosis. Among its related pathways are Transport to the Golgi and subsequent modification and HIV Life Cycle. GO annotations related to this gene include ligase activity. An important paralog of this gene is RNF5.

UniProtKB/Swiss-Prot for RNF185 Gene

  • E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF185 Gene

Genomics for RNF185 Gene

Regulatory Elements for RNF185 Gene

Enhancers for RNF185 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH22F031209 1.1 FANTOM5 ENCODE 24.3 +51.5 51463 4.3 HDGF PKNOX1 MLX ZFP64 ARID4B SIN3A DMAP1 YY1 SLC30A9 ZNF143 LIMK2 RNF185 ENSG00000214125 PLA2G3 PIK3IP1 SMTN RNF185-AS1
GH22F031214 1.2 FANTOM5 Ensembl ENCODE 18.1 +59.6 59579 10.1 HDGF PKNOX1 ARID4B FEZF1 DMAP1 ZNF48 YY1 ZNF121 GLIS2 FOS LIMK2 PLA2G3 RNF185 SFI1 ENSG00000214125 PIK3IP1 EIF4ENIF1 GC22M031223 RNU6-1128P
GH22F031206 1.1 ENCODE 17.2 +47.2 47222 0.8 HDAC1 ATF1 PKNOX1 TBL1XR1 SIN3A ZNF766 GATA2 RCOR1 CREM ZBTB11 RNF185 ENSG00000214125 PLA2G3 LIMK2 RNF185-AS1
GH22F031226 1.2 FANTOM5 Ensembl ENCODE 16.2 +71.4 71418 9.7 HDGF PKNOX1 CREB3L1 ARNT MLX ARID4B SIN3A DMAP1 YY1 ZNF766 LIMK2 RNF185 SFI1 SELENOM INPP5J PATZ1 LINC01521 PIK3IP1 SMTN ENSG00000202019
GH22F031191 0.7 ENCODE 16 +32.6 32611 1.5 TBP ELF3 ARID4B ZSCAN9 ZNF48 YY1 RARA CREM MIXL1 CEBPB RNF185 ENSG00000214125 PLA2G3 RNU6-1128P INPP5J SELENOM SFI1 PIR51329
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around RNF185 on UCSC Golden Path with GeneCards custom track

Promoters for RNF185 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001843014 349 2200 PKNOX1 ARNT WRNIP1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF143

Genomic Location for RNF185 Gene

Chromosome:
22
Start:
31,160,152 bp from pter
End:
31,207,019 bp from pter
Size:
46,868 bases
Orientation:
Plus strand

Genomic View for RNF185 Gene

Genes around RNF185 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF185 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF185 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF185 Gene

Proteins for RNF185 Gene

  • Protein details for RNF185 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96GF1-RN185_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF185
    Protein Accession:
    Q96GF1
    Secondary Accessions:
    • A8K5C1
    • A9X3T8
    • Q8N900

    Protein attributes for RNF185 Gene

    Size:
    192 amino acids
    Molecular mass:
    20459 Da
    Quaternary structure:
    • Interacts with ATG5 and BNIP1.

    Alternative splice isoforms for RNF185 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF185 Gene

Post-translational modifications for RNF185 Gene

  • Ubiquitination at Lys 80 and Lys 87
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF185 Gene

No data available for DME Specific Peptides for RNF185 Gene

Domains & Families for RNF185 Gene

Gene Families for RNF185 Gene

Protein Domains for RNF185 Gene

Graphical View of Domain Structure for InterPro Entry

Q96GF1

UniProtKB/Swiss-Prot:

RN185_HUMAN :
  • The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.
  • Contains 1 RING-type zinc finger.
Domain:
  • The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RNF185: view

Function for RNF185 Gene

Molecular function for RNF185 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
UniProtKB/Swiss-Prot Induction:
Up-regulated by unfolded protein response (UPR) and ER stress.

Enzyme Numbers (IUBMB) for RNF185 Gene

Gene Ontology (GO) - Molecular Function for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 19549727
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0044390 ubiquitin-like protein conjugating enzyme binding IPI 24019521
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with RNF185: view
genes like me logo Genes that share phenotypes with RNF185: view

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNF185 Gene

Localization for RNF185 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF185 Gene

Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF185 Gene COMPARTMENTS Subcellular localization image for RNF185 gene
Compartment Confidence
endoplasmic reticulum 5
mitochondrion 5
nucleus 3
peroxisome 2

Gene Ontology (GO) - Cellular Components for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IEA --
GO:0005741 mitochondrial outer membrane IEA --
GO:0005783 endoplasmic reticulum IDA 24019521
GO:0005789 endoplasmic reticulum membrane NAS 24019521
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with RNF185: view

Pathways & Interactions for RNF185 Gene

genes like me logo Genes that share pathways with RNF185: view

UniProtKB/Swiss-Prot Q96GF1-RN185_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006914 autophagy IEA --
GO:0016567 protein ubiquitination IEA --
GO:0030433 ER-associated ubiquitin-dependent protein catabolic process IMP 24019521
GO:0036503 ERAD pathway IGI 24019521
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process IBA --
genes like me logo Genes that share ontologies with RNF185: view

No data available for SIGNOR curated interactions for RNF185 Gene

Transcripts for RNF185 Gene

Unigene Clusters for RNF185 Gene

Ring finger protein 185:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF185 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c ^ 11a · 11b ·
SP1: - - - - - - - - -
SP2: - - - - - - - - - -
SP3: - - - - - -
SP4: -
SP5: - - - - - - - - - - - -
SP6: -
SP7: - - -
SP8: - - - - - - -
SP9: - - - - - - -
SP10: - - - - - - -
SP11: - - - - - - -
SP12: - - - - - -
SP13: - - - - - - -
SP14: - - - - - - -
SP15: - - - - - - - - - - - - - -
SP16: - - - - - -
SP17:
SP18: - -

ExUns: 11c · 11d
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:
SP18:

Relevant External Links for RNF185 Gene

GeneLoc Exon Structure for
RNF185
ECgene alternative splicing isoforms for
RNF185

Expression for RNF185 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF185 Gene

Protein differential expression in normal tissues from HIPED for RNF185 Gene

This gene is overexpressed in Bone (18.5), Pancreas (8.1), and Lung (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for RNF185 Gene



Protein tissue co-expression partners for RNF185 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RNF185 Gene:

RNF185

SOURCE GeneReport for Unigene cluster for RNF185 Gene:

Hs.517553
genes like me logo Genes that share expression patterns with RNF185: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RNF185 Gene

Orthologs for RNF185 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF185 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF185 34 35
  • 100 (n)
oppossum
(Monodelphis domestica)
Mammalia RNF185 35
  • 95 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RNF185 35
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF185 34 35
  • 94.62 (n)
mouse
(Mus musculus)
Mammalia Rnf185 34 16 35
  • 93.06 (n)
cow
(Bos Taurus)
Mammalia RNF185 34 35
  • 92.71 (n)
rat
(Rattus norvegicus)
Mammalia Rnf185 34
  • 92.36 (n)
chicken
(Gallus gallus)
Aves RNF185 34 35
  • 82.12 (n)
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4501 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.13633 34
zebrafish
(Danio rerio)
Actinopterygii rnf185 34 35
  • 73.26 (n)
wufk32c04 34
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8264 34
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006041 34
  • 57.92 (n)
fruit fly
(Drosophila melanogaster)
Insecta CG8974 34 35
  • 57.46 (n)
CG32847 35
  • 43 (a)
ManyToMany
CG32581 35
  • 37 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea rnf-5 34 35
  • 54.64 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2160 34
Species where no ortholog for RNF185 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RNF185 Gene

ENSEMBL:
Gene Tree for RNF185 (if available)
TreeFam:
Gene Tree for RNF185 (if available)

Paralogs for RNF185 Gene

Paralogs for RNF185 Gene

(1) SIMAP similar genes for RNF185 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for RNF185 Gene

genes like me logo Genes that share paralogs with RNF185: view

Variants for RNF185 Gene

Sequence variations from dbSNP and Humsavar for RNF185 Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type
rs1034589 -- 31,183,247(-) acact(A/G)atcaa intron-variant
rs10644354 -- 31,207,501(+) CAATC(-/AT)AGGTT downstream-variant-500B
rs10710631 -- 31,199,100(+) GTCTC(-/A)AAAAA intron-variant
rs11089491 -- 31,196,712(+) GAATA(A/T)CTCAT intron-variant
rs111227051 -- 31,171,427(+) CCACC(C/T)GCCTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF185 Gene

Variant ID Type Subtype PubMed ID
esv3568357 CNV loss 25503493
nsv1110864 OTHER inversion 24896259
nsv459871 CNV loss 19166990
nsv507933 OTHER sequence alteration 20534489
nsv588893 CNV loss 21841781
nsv979669 CNV duplication 23825009

Variation tolerance for RNF185 Gene

Residual Variation Intolerance Score: 47.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.29; 6.48% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RNF185 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF185

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF185 Gene

Disorders for RNF185 Gene

MalaCards: The human disease database

(1) MalaCards diseases for RNF185 Gene - From: GeneCards

Disorder Aliases PubMed IDs
cystic fibrosis
  • pseudomonas aeruginosa chronic infection by, in cystic fibrosis
- elite association - COSMIC cancer census association via MalaCards

Relevant External Links for RNF185

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RNF185
genes like me logo Genes that share disorders with RNF185: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RNF185 Gene

Publications for RNF185 Gene

  1. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR). (PMID: 24019521) El Khouri E. … Delaunay-Moisan A. (J. Biol. Chem. 2013) 3 4 64
  2. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. (PMID: 21931693) Tang F. … Tang J. (PLoS ONE 2011) 3 4 64
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 64
  4. A genome annotation-driven approach to cloning the human ORFeome. (PMID: 15461802) Collins J.E. … Dunham I. (Genome Biol. 2004) 3 4 64
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64

Products for RNF185 Gene

Sources for RNF185 Gene

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