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Aliases for RNF185 Gene

Aliases for RNF185 Gene

  • Ring Finger Protein 185 2 3
  • Hypothetical Protein FLJ38628 2
  • RING Finger Protein 185 4
  • BSK65-MONO1 3
  • BSK65-MONO2 3
  • BSK65-PANC1 3
  • BSK65-PANC2 3
  • BSK65-TEST1 3
  • BSK65-TEST2 3
  • BSK65-TEST3 3
  • EC 6.3.2.- 4

External Ids for RNF185 Gene

Previous GeneCards Identifiers for RNF185 Gene

  • GC22P029881
  • GC22P031556
  • GC22P014517

Summaries for RNF185 Gene

GeneCards Summary for RNF185 Gene

RNF185 (Ring Finger Protein 185) is a Protein Coding gene. Among its related pathways are Protein processing in endoplasmic reticulum. GO annotations related to this gene include ligase activity. An important paralog of this gene is RNF5.

UniProtKB/Swiss-Prot for RNF185 Gene

  • E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF185 Gene

Genomics for RNF185 Gene

Regulatory Elements for RNF185 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RNF185 Gene

Chromosome:
22
Start:
31,160,152 bp from pter
End:
31,207,019 bp from pter
Size:
46,868 bases
Orientation:
Plus strand

Genomic View for RNF185 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RNF185 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF185 Gene

Proteins for RNF185 Gene

  • Protein details for RNF185 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96GF1-RN185_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF185
    Protein Accession:
    Q96GF1
    Secondary Accessions:
    • A8K5C1
    • A9X3T8
    • Q8N900

    Protein attributes for RNF185 Gene

    Size:
    192 amino acids
    Molecular mass:
    20459 Da
    Quaternary structure:
    • Interacts with ATG5 and BNIP1.

    Alternative splice isoforms for RNF185 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF185 Gene

Proteomics data for RNF185 Gene at MOPED

Post-translational modifications for RNF185 Gene

  • Ubiquitination at Lys 80 and Lys 87
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF185 Gene

Antibody Products

No data available for DME Specific Peptides for RNF185 Gene

Domains & Families for RNF185 Gene

Gene Families for RNF185 Gene

Protein Domains for RNF185 Gene

Graphical View of Domain Structure for InterPro Entry

Q96GF1

UniProtKB/Swiss-Prot:

RN185_HUMAN :
  • The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity
  • Contains 1 RING-type zinc finger.
Domain:
  • The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RNF185: view

Function for RNF185 Gene

Molecular function for RNF185 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
UniProtKB/Swiss-Prot Induction:
Up-regulated by unfolded protein response (UPR) and ER stress.

Enzyme Numbers (IUBMB) for RNF185 Gene

Gene Ontology (GO) - Molecular Function for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 19549727
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0044390 ubiquitin-like protein conjugating enzyme binding IPI 24019521
GO:1904264 ubiquitin protein ligase activity involved in ERAD pathway IDA 24019521
genes like me logo Genes that share ontologies with RNF185: view

Phenotypes for RNF185 Gene

GenomeRNAi human phenotypes for RNF185:
genes like me logo Genes that share phenotypes with RNF185: view

Animal Model Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RNF185

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targets and HOMER Transcription for RNF185 Gene

Localization for RNF185 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF185 Gene

Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF185 Gene COMPARTMENTS Subcellular localization image for RNF185 gene
Compartment Confidence
endoplasmic reticulum 5
mitochondrion 5

Gene Ontology (GO) - Cellular Components for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005741 mitochondrial outer membrane IEA --
GO:0005783 endoplasmic reticulum IDA 24019521
GO:0005789 endoplasmic reticulum membrane NAS 24019521
GO:0016021 integral component of membrane IEA --
GO:0036513 colocalizes_with Derlin-1 retrotranslocation complex IDA 24019521
genes like me logo Genes that share ontologies with RNF185: view

Pathways & Interactions for RNF185 Gene

genes like me logo Genes that share pathways with RNF185: view

Pathways by source for RNF185 Gene

1 KEGG pathway for RNF185 Gene

UniProtKB/Swiss-Prot Q96GF1-RN185_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006914 autophagy IEA --
GO:0030433 ER-associated ubiquitin-dependent protein catabolic process IMP 24019521
GO:0036503 ERAD pathway IGI 24019521
GO:0051865 protein autoubiquitination IDA 24019521
genes like me logo Genes that share ontologies with RNF185: view

No data available for SIGNOR curated interactions for RNF185 Gene

Drugs & Compounds for RNF185 Gene

No Compound Related Data Available

Transcripts for RNF185 Gene

Unigene Clusters for RNF185 Gene

Ring finger protein 185:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RNF185

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF185 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c ^ 11a · 11b ·
SP1: - - - - - - - - -
SP2: - - - - - - - - - -
SP3: - - - - - -
SP4: -
SP5: - - - - - - - - - - - -
SP6: -
SP7: - - -
SP8: - - - - - - -
SP9: - - - - - - -
SP10: - - - - - - -
SP11: - - - - - - -
SP12: - - - - - -
SP13: - - - - - - -
SP14: - - - - - - -
SP15: - - - - - - - - - - - - - -
SP16: - - - - - -
SP17:
SP18: - -

ExUns: 11c · 11d
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:
SP18:

Relevant External Links for RNF185 Gene

GeneLoc Exon Structure for
RNF185
ECgene alternative splicing isoforms for
RNF185

Expression for RNF185 Gene

mRNA expression in normal human tissues for RNF185 Gene

Protein differential expression in normal tissues from HIPED for RNF185 Gene

This gene is overexpressed in Bone (18.5), Pancreas (8.1), and Lung (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for RNF185 Gene



SOURCE GeneReport for Unigene cluster for RNF185 Gene Hs.517553

genes like me logo Genes that share expression patterns with RNF185: view

Protein tissue co-expression partners for RNF185 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RNF185 Gene

Orthologs for RNF185 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF185 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RNF185 35
  • 92.71 (n)
  • 98.44 (a)
RNF185 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF185 35
  • 94.62 (n)
  • 100 (a)
RNF185 36
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf185 35
  • 93.06 (n)
  • 98.44 (a)
Rnf185 16
Rnf185 36
  • 83 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RNF185 35
  • 100 (n)
  • 100 (a)
RNF185 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf185 35
  • 92.36 (n)
  • 98.96 (a)
oppossum
(Monodelphis domestica)
Mammalia RNF185 36
  • 95 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RNF185 36
  • 95 (a)
OneToOne
chicken
(Gallus gallus)
Aves RNF185 35
  • 82.12 (n)
  • 92.19 (a)
RNF185 36
  • 93 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4501 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.13633 35
zebrafish
(Danio rerio)
Actinopterygii rnf185 35
  • 73.26 (n)
  • 86.46 (a)
wufk32c04 35
rnf185 36
  • 86 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8264 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006041 35
  • 57.92 (n)
  • 58.12 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG8974 35
  • 57.46 (n)
  • 61.96 (a)
CG32581 36
  • 37 (a)
ManyToMany
CG32847 36
  • 43 (a)
ManyToMany
CG8974 36
  • 40 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea rnf-5 35
  • 54.64 (n)
  • 55.06 (a)
rnf-5 36
  • 39 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2160 35
Species with no ortholog for RNF185:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RNF185 Gene

ENSEMBL:
Gene Tree for RNF185 (if available)
TreeFam:
Gene Tree for RNF185 (if available)

Paralogs for RNF185 Gene

Paralogs for RNF185 Gene

(1) SIMAP similar genes for RNF185 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for RNF185 Gene

genes like me logo Genes that share paralogs with RNF185: view

Variants for RNF185 Gene

Sequence variations from dbSNP and Humsavar for RNF185 Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type MAF
rs6651 -- 31,206,786(-) GCCGA(A/G)GGATG utr-variant-3-prime, nc-transcript-variant
rs8242 -- 31,206,882(-) CCACA(A/C)GGGTG utr-variant-3-prime, nc-transcript-variant
rs8595 -- 31,206,655(-) GCCAG(C/G)CTGGC utr-variant-3-prime, nc-transcript-variant
rs13158 -- 31,206,201(+) CTTCA(C/T)AGTCC utr-variant-3-prime, nc-transcript-variant
rs886074 -- 31,179,304(-) ATGGG(C/T)CTACC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF185 Gene

Variant ID Type Subtype PubMed ID
nsv459871 CNV Loss 19166990
nsv507933 CNV Insertion 20534489

Variation tolerance for RNF185 Gene

Residual Variation Intolerance Score: 47.58% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.29; 6.48% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RNF185 Gene

HapMap Linkage Disequilibrium report
RNF185

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF185 Gene

Disorders for RNF185 Gene

Relevant External Links for RNF185

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RNF185
genes like me logo Genes that share disorders with RNF185: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF185 Gene

Publications for RNF185 Gene

  1. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PMID: 12477932) Strausberg R.L. … Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002) 2 67
  2. Quantitative Lys-I/-Gly-Gly (diGly) proteomics coupled with inducible RNAi reveals ubiquitin-mediated proteolysis of DNA damage-inducible transcript 4 (DDIT4) by the E3 ligase HUWE1. (PMID: 25147182) Thompson J.W. … Luchansky S.J. (J. Biol. Chem. 2014) 67
  3. A proteome-scale map of the human interactome network. (PMID: 25416956) Rolland T. … Vidal M. (Cell 2014) 67
  4. The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation. (PMID: 24727453) Zhou Y. … Hou S. (Biochem. Biophys. Res. Commun. 2014) 67
  5. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR). (PMID: 24019521) El Khouri E. … Delaunay-Moisan A. (J. Biol. Chem. 2013) 67

Products for RNF185 Gene

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