RNF181 Gene
protein-coding GIFtS: 46
GCID: GC02P085822
|
|
ring finger protein 181
| |
Aliases
for RNF181 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Ring Finger Protein 1811 2 3 | | HSPC2381 | | E3 Ubiquitin-Protein Ligase RNF1812 | | EC 6.3.2.-3 |
Export aliases for RNF181 gene to outside databasesPrevious GC identifers: GC02P085676 GC02P085719 |
Summaries
for RNF181 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for RNF181:
RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligaseactivity (Brophy et al., 2008 (PubMed 18331836)).(supplied by OMIM, Dec 2008)
UniProtKB/Swiss-Prot: RN181_HUMAN, Q9P0P0Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of athioester and then directly transfers the ubiquitin to targeted substrates
|
Genomic Views
for RNF181 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000002.11 NC_018913.1 NT_022184.15
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the RNF181 gene promoter:
c-Fos AP-1 FosB Nkx2-5 E47 FOXC1 E2F E2F-1 Msx-1 c-Jun
Other transcription factors
Search SABiosciences Chromatin IP Primers for RNF181
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNF181 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 2p11.2 Ensembl cytogenetic band: 2p11.2 HGNC cytogenetic band: 2p11.2RNF181 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 2 GeneLoc Exon Structure GeneLoc location for GC02P085822: view genomic region
(about GC identifiers)
Start:
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85,822,837 bp from pter |
End:
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85,824,831 bp from pter |
Size:
|
1,995 bases |
Orientation:
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plus strand |
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Proteins
for RNF181 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RN181_HUMAN, Q9P0P0 (See
protein sequence)Recommended Name: E3 ubiquitin-protein ligase RNF181 Size: 153 amino acids; 17909 Da
Subunit: Directly interacts with ITGA2B and, as a result, with integrin ITGA2B/ITGB3. There is no evidence thatintegrin ITGA2B/ITGB3 is an endogenous substrate for RNF181-directed ubiquitination
Secondary accessions: Q53H81Explore the universe of human proteins at neXtProt for RNF181: NX_Q9P0P0
Post-translational modifications:
Auto-ubiquitinated as part of the enzymatic reaction1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9P0P0
RNF181 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_057578.1
ENSEMBL proteins: ENSP00000412025 ENSP00000306906 ENSP00000397109 ENSP00000391326 ENSP00000415246
Human Recombinant Protein Products:
RNF181 Antibody Products:
Assay Products for RNF181: |
Protein
Domains /
Families
for RNF181 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RNF181 for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9P0P0ProtoNet protein and cluster: Q9P0P0 1 Blocks protein family: IPB001841 Zn-finger
UniProtKB/Swiss-Prot: RN181_HUMAN, Q9P0P0Similarity: Belongs to the RNF181 familySimilarity: Contains 1 RING-type zinc finger |
Function
for RNF181 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RN181_HUMAN, Q9P0P0Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of athioester and then directly transfers the ubiquitin to targeted substratesEnzyme Number (IUBMB): EC 6.3.2.-1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF181 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF181 (see all 2)
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Synthesis Service for RNF181 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF181 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0008270 | zinc ion binding |
IEA | -- | | GO:0016874 | ligase activity |
IEA | -- |
RNF181 for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for RNF181:
|
Pathways & Interactions
for RNF181 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: RN181_HUMAN, Q9P0P0Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RNF181
STRING Interaction
Network Preview (showing 5 interactants - click image to see 19)
5/20 Interacting proteins for RNF181 (Q9P0P03 ENSP000003069064) via UniProtKB, MINT, STRING, and/or I2D (see all 20) About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016567 | protein ubiquitination |
IEA | -- |
RNF181 for ontologies About GeneDecksing
|
Drugs & Compounds
for RNF181 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for RNF181
Search CenterWatch for drugs/clinical trials and news about RNF181 / RN181
|
Transcripts
for RNF181 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for RNF181 gene: NM_016494.3 Unigene Cluster for RNF181: Ring finger protein 181 Hs.356187 [show with all ESTs]Unigene Representative Sequence: CD2423986 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000461845 ENST00000441634(uc002spv.1) ENST00000306368 ENST00000414390
ENST00000443647 ENST00000456023
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF181 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF181 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: RNF181 (NM_016494) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RNF181 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF181 |
Additional cDNA sequence: AF151072.1 AK222700.1 BC002803.2 CR457165.1 15 DOTS entries: DT.449021 DT.100824128 DT.95174634 DT.100824132 DT.95174642 DT.100824130 DT.95174636 DT.100032387 DT.99954127 DT.100036784 DT.120929939 DT.92436844 DT.92436847 DT.95174637 DT.97859068 24/265 AceView cDNA sequences (see all 265): AI002474 AI150900 BG488504 AA888074 AW961040 BF222633 AA815301 AI015971 H73233 AA035385 AI885762 BQ219907 AA370418 AI826700 CD677155 CA950240 H69071 AA887224 H47715 CA413633 AA938754 AI658663 AW205448 AI685629
GeneLoc Exon Structure
5/8 Alternative Splicing Database (ASD) splice patterns (SP) for RNF181 (see all 8) About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | · | 1e | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6 | ^ | 7a | · | 7b | · | 7c |
|---|
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| SP1: | | | | | | | | | | | | | - | | | | | | - | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | - | | | | | | - | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | - | | - | | | | - | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | - | | - | | - | | - | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | - | | | | | | - | | - | | | | | | | |
ECgene alternative splicing isoforms for RNF181
|
Expression
for RNF181 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RNF181 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TACACGTGAG
About this image
See RNF181 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RNF181
SOURCE GeneReport for Unigene cluster: Hs.356187
UniProtKB/Swiss-Prot: RN181_HUMAN, Q9P0P0Tissue specificity: Widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletalmuscle. Expressed in platelets (at protein level)
SABiosciences Expression via Pathway-Focused PCR Array including RNF181:
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RNF181
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RNF181 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNF181 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNF181 |
Orthologs
for RNF181 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for RNF181 gene from 5/18 species (see all 18) About this table
ENSEMBL Gene Tree for RNF181 (if available)
TreeFam Gene Tree for RNF181 (if available) |
Paralogs
for RNF181 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for RNF181 gene
- PJA22 RNF1152 RNF1262 PJA12
7 SIMAP similar genes for RNF181 using alignment to 6 protein entries: RN181_HUMAN (see all proteins):PJA2 RNF115 RNF167 PJA1 RNF126 SCAF11
TRIM39
RNF181 for paralogs About GeneDecksing
2 Pseudogenes.org Pseudogenes for RNF181
PGOHUM00000242531 PGOHUM00000233516
|
Genomic Variants
for RNF181 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 2 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for RNF181 (85822837 - 85824831 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for RNF181: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RNF181 |
|
Disorders
/ Diseases
for RNF181 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RNF181 for disorders About GeneDecksing
OMIM gene information: 612490
OMIM disorders: --
4  diseases for RNF181: About MalaCardsintrahepatic cholangiocarcinoma cholangiocarcinoma hepatocellular carcinoma carcinoma
Export disorders for RNF181 gene to outside databases
|
Publications
for RNF181 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RNF181 gene, integrated from 9 sources (see all 18):
(articles sorted by number of sources associating them with RNF181) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. (PubMed id 11042152)1, 2, 3 Zhang Q.-H.... Chen Z. (2000)
- RN181, a novel ubiquitin E3 ligase that interacts with the KVGFFKR motif of platelet integrin alpha(IIb)beta3. (PubMed id 18331836)1, 2 Brophy T.M....Moran N. (2008)
- Systematic analysis of dimeric E3-RING interactions re veals increased combinatorial complexity in human ubiquitination networks. (PubMed id 22493164)1 Woodsmith J....Sanderson C.M. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- RN181 suppresses hepatocellular carcinoma growth by i nhibition of the ERK/MAPK pathway. (PubMed id 21391225)1 Wang S....Li M. (2011)
- Seven prostate cancer susceptibility loci identified b y a multi-stage genome-wide association study. (PubMed id 21743467)1 Kote-Jarai Z.... . (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
- [Construction of recombinant eukaryotic expression ve ctor pDsRed1-C3/LOC51255 and subcellular localization of LOC51255 protein in He PG2 cell line] (PubMed id 19426595)1 Huang X....Li M. (2009)
- A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. (PubMed id 19690564)1 van Wijk S.J....Timmers H.T. (2009)
|
External Searches for RNF181 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing RNF181 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RNF181 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RNF181 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for RNF181 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for RNF181 gene:
Search GeneIP for patents involving RNF181
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for RNF181 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for RNF181 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RNF181 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RNF181 | | | OriGene Protein Over-expression Lysate for RNF181 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for RNF181 | | OriGene 3'-UTR Clone for RNF181 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF181 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF181 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for RNF181 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RNF181 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RNF181 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNF181 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RNF181 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNF181 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNF181 |
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