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RNF175 Gene

protein-coding   GIFtS: 46
GCID: GC04M154631

Ring Finger Protein 175

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ring Finger Protein 1751 2

External Ids:    HGNC: 277351   Entrez Gene: 2855332   Ensembl: ENSG000001454287   UniProtKB: Q8N4F73   

Export aliases for RNF175 gene to outside databases

Previous GC identifers: GC04M154989 GC04M154850 GC04M150362


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for RNF175 Gene:
RNF175 (ring finger protein 175) is a protein-coding gene. An important paralog of this gene is RNF121.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000004.11  NT_016354.20  NC_018915.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the RNF175 gene promoter:
         AML1a   ATF-2   NF-AT   C/EBPalpha   FAC1   NF-AT2   Sox9   NF-AT1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRNF175 promoter sequence
   Search Chromatin IP Primers for RNF175

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RNF175


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q31.3   Ensembl cytogenetic band:  4q31.3   HGNC cytogenetic band: 4q31.3

RNF175 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RNF175 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M154631:  view genomic region     (about GC identifiers)

Start:
154,631,277 bp from pter      End:
154,681,387 bp from pter
Size:
50,111 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: RN175_HUMAN, Q8N4F7 (See protein sequence)
Recommended Name: RING finger protein 175  
Size: 328 amino acids; 38266 Da

Explore the universe of human proteins at neXtProt for RNF175: NX_Q8N4F7

Explore proteomics data for RNF175 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See RNF175 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_775933.1  
    ENSEMBL proteins: 
     ENSP00000340979   ENSP00000421761   ENSP00000424430   ENSP00000427472   ENSP00000424563  
     ENSP00000274068  

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    antibodies-online peptides for RNF175

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    antibodies-online kits for RNF175 (2 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    RNF: RING-type (C3HC4) zinc fingers

    2 InterPro protein domains:
     IPR001841 Znf_RING
     IPR013083 Znf_RING/FYVE/PHD

    Graphical View of Domain Structure for InterPro Entry Q8N4F7

    ProtoNet protein and cluster: Q8N4F7

    1 Blocks protein domain: IPB001841 Zn-finger

    UniProtKB/Swiss-Prot: RN175_HUMAN, Q8N4F7
    Similarity: Contains 1 RING-type zinc finger


    Find genes that share domains with RNF175           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding ----
    GO:0008270zinc ion binding IEA--
         
    Find genes that share ontologies with RNF175           About GenesLikeMe


    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for RNF175

    miRNA
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    2 qRT-PCR Assays for microRNAs that regulate RNF175:
    hsa-miR-450b-3p hsa-miR-3692
    SwitchGear 3'UTR luciferase reporter plasmidRNF175 3' UTR sequence
    Inhib. RNA
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    RN175_HUMAN, Q8N4F7: Membrane; Multi-pass membrane protein (Potential)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane3
    peroxisome2

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016021integral component of membrane IEA--

    Find genes that share ontologies with RNF175           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Pathway & Disease-focused RT2 Profiler PCR Array including RNF175: 
              Ubiquitin Ligases in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for RNF175

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for RNF175 (RN175)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for RNF175 gene: 
    NM_173662.2  

    Unigene Cluster for RNF175:

    Ring finger protein 175
    Hs.388364  [show with all ESTs]
    Unigene Representative Sequence: AK091509
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000347063(uc003int.3) ENST00000513656 ENST00000503694 ENST00000508248
    ENST00000508967 ENST00000506505 ENST00000507512 ENST00000509321 ENST00000506358
    ENST00000274068(uc003inu.1)
    miRNA
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    2 qRT-PCR Assays for microRNAs that regulate RNF175:
    hsa-miR-450b-3p hsa-miR-3692
    SwitchGear 3'UTR luciferase reporter plasmidRNF175 3' UTR sequence
    Inhib. RNA
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    Primer
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    OriGene qPCR primer pairs and template standards for RNF175
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      QuantiTect SYBR Green Assays in human, mouse, rat RNF175
      QuantiFast Probe-based Assays in human, mouse, rat RNF175

    Additional mRNA sequence: 

    AK091509.1 BC034385.1 

    6 DOTS entries:

    DT.209717  DT.100749914  DT.121276038  DT.100018360  DT.121276043  DT.92057043 

    Selected AceView cDNA sequences (see all 37):

    BX279796 BI819244 F10085 BM013968 H09541 BM669323 R59473 BC034385 
    BU734824 BI522497 BM013820 BI823123 BM712498 AK091509 BG202387 AI142621 
    F12464 AI365130 AX747029 BI551533 AV720984 NM_173662 AI023856 BQ638728 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for RNF175 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c ^ 9 ^ 10
    SP1:                                -                 -                                         -     -         
    SP2:                                -                 -     -                                                   
    SP3:                                -     -     -     -                                                         
    SP4:                                                                                                            
    SP5:                                                        -                                                   


    ECgene alternative splicing isoforms for RNF175

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    RNF175 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTTTTTCATC
    RNF175 Expression
    About this image

    RNF175 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RNF175 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.388364
        Pathway & Disease-focused RT2 Profiler PCR Array including RNF175: 
              Ubiquitin Ligases in human mouse rat

    Primer
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for RNF175 gene from Selected species (see all 7)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    dog
    (Canis familiaris)
    Mammalia RNF1751 ring finger protein 175 90.15(n)
    94.81(a)
      612478  XM_850209.3  XP_855302.2 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc566652 hypothetical protein MGC56665 75.22(n)   393278  BC050174.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG158146
    --
    55(a)
    1 → many
    X(17742143-17744396)
    worm
    (Caenorhabditis elegans)
    Secernentea rnf-1216
    Protein RNF-121 (rnf-121) mRNA, complete cds
    37(a)
    1 → many
    III(4168797-4171161) WBGene00007626


    ENSEMBL Gene Tree for RNF175 (if available)
    TreeFam Gene Tree for RNF175 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for RNF175 gene
    RNF1212  
    1 SIMAP similar gene for RNF175 using alignment to 6 protein entries:     RN175_HUMAN (see all proteins):
    RNF121

    Find genes that share paralogs with RNF175           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for RNF175
    PGOHUM00000249537


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RNF175 (see all 1092)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs561883021,2
    C--150370401(+) TGTTTT/GTTTGT 1 -- int11Minor allele frequency- G:0.00WA 2
    rs3733279931,2
    C--150370401(+) TGTTT-/GTTTGT 1 -- int10--------
    rs25799111,2
    C,A,H--150371961(-) CTATAG/TCTGAT 1 -- int10--------
    rs26063211,2
    C,F,A,H--150371961(+) CATCAA/GCTATA 1 -- int16Minor allele frequency- G:0.45NA WA EA 366
    rs559162461,2
    F--150371962(+) ATCAAC/ATATAG 1 -- int15Minor allele frequency- A:0.44NA WA EA 364
    rs2020627891,2
    --150371962(+) ATCAA-/CTATAG 1 -- int10--------
    rs1814052661,2
    --150377172(+) GGAGTA/GGGCAG 1 -- int10--------
    rs1864272511,2
    C--150377269(+) AGCTGC/TGTGGG 1 -- int10--------
    rs1909183491,2
    C--150377306(+) GGGAGC/TGGGCA 1 -- int10--------
    rs97626151,2
    C--150377317(+) GGGGTA/GGGCTG 1 -- int10--------

    HapMap Linkage Disequilibrium report for RNF175 (154631277 - 154681387 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 6 variations for RNF175:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2728557CNV Deletion23290073
    esv2728558CNV Deletion23290073
    esv2728556CNV Deletion23290073
    esv2728555CNV Deletion23290073
    nsv482972CNV Gain15286789
    nsv10589CNV Gain18304495

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing RNF175
    DNA2.0 Custom Variant and Variant Library Synthesis for RNF175

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    Find genes that share disorders with RNF175           About GenesLikeMe

    Genetic Association Database (GAD): RNF175
    Human Genome Epidemiology (HuGE) Navigator: RNF175 (5 documents)

    Export disorders for RNF175 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RNF175 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with RNF175)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    2. Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. (PubMed id 19773279)1, 4 Hosgood H.D....Lan Q. (Occup Environ Med 2009)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    4. Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph. (PubMed id 21048031)1 Casabonne D....de Sanjose S. (Haematologica 2011)
    5. Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. (PubMed id 19913121)1 Talmud P.J.... . (Am. J. Hum. Genet. 2009)
    6. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (Genomics 2007)
    7. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W.... Wilson R.K. (Nature 2005)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1 Ota T.... Sugano S. (Nat. Genet. 2004)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 285533 HGNC: 27735 AceView: LOC285533 Ensembl:ENSG00000145428 euGenes: HUgn285533
    ECgene: RNF175 H-InvDB: RNF175

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for RNF175 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RNF175 gene:
    Search GeneIP for patents involving RNF175

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from genOway)
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