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Aliases for RNF169 Gene

Aliases for RNF169 Gene

  • Ring Finger Protein 169 2 3
  • RING Finger Protein 169 4
  • EC 3.4.24.57 63
  • EC 3.1.4.16 63
  • EC 6.3.2.- 4
  • KIAA1991 4

External Ids for RNF169 Gene

Previous GeneCards Identifiers for RNF169 Gene

  • GC11P074138
  • GC11P074459
  • GC11P070756

Summaries for RNF169 Gene

GeneCards Summary for RNF169 Gene

RNF169 (Ring Finger Protein 169) is a Protein Coding gene. GO annotations related to this gene include ligase activity and nucleosome binding.

UniProtKB/Swiss-Prot for RNF169 Gene

  • Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF169 Gene

Genomics for RNF169 Gene

Regulatory Elements for RNF169 Gene

Genomic Location for RNF169 Gene

Start:
74,748,868 bp from pter
End:
74,842,413 bp from pter
Size:
93,546 bases
Orientation:
Plus strand

Genomic View for RNF169 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RNF169 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF169 Gene

Proteins for RNF169 Gene

  • Protein details for RNF169 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8NCN4-RN169_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF169
    Protein Accession:
    Q8NCN4
    Secondary Accessions:
    • Q6N015

    Protein attributes for RNF169 Gene

    Size:
    708 amino acids
    Molecular mass:
    77194 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=BAC02700.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for RNF169 Gene

Proteomics data for RNF169 Gene at MOPED

Selected DME Specific Peptides for RNF169 Gene

Post-translational modifications for RNF169 Gene

  • Ubiquitination at Lys691
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF169 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains for RNF169 Gene

Gene Families for RNF169 Gene

HGNC:
  • RNF :RING-type (C3HC4) zinc fingers

Protein Domains for RNF169 Gene

Suggested Antigen Peptide Sequences for RNF169 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8NCN4

UniProtKB/Swiss-Prot:

RN169_HUMAN :
  • Q8NCN4
Domain:
  • The MIU motif (motif interacting with ubiquitin) mediates the interaction with both Lys-48- and Lys-63-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
Family:
  • Belongs to the RNF169 family.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RNF169: view

Function for RNF169 Gene

Molecular function for RNF169 Gene

UniProtKB/Swiss-Prot Function: Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.

Enzyme Numbers (IUBMB) for RNF169 Gene

Gene Ontology (GO) - Molecular Function for RNF169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding --
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0031491 nucleosome binding IDA 22742833
GO:0070530 K63-linked polyubiquitin binding IDA 22492721
genes like me logo Genes that share ontologies with RNF169: view
genes like me logo Genes that share phenotypes with RNF169: view

No data available for Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for RNF169 Gene

Localization for RNF169 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF169 Gene

Nucleus, nucleoplasm. Note=Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF169 Gene COMPARTMENTS Subcellular localization image for RNF169 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for RNF169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 22733822
GO:0005654 nucleoplasm IDA --
GO:0005886 plasma membrane --
GO:0035861 site of double-strand break IDA 22492721
GO:0043231 intracellular membrane-bounded organelle --
genes like me logo Genes that share ontologies with RNF169: view

Pathways for RNF169 Gene

SuperPathways for RNF169 Gene

No Data Available

UniProtKB/Swiss-Prot Q8NCN4-RN169_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for RNF169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006974 cellular response to DNA damage stimulus IMP 22492721
GO:0016567 protein ubiquitination IEA --
GO:2000780 negative regulation of double-strand break repair IMP 22492721
genes like me logo Genes that share ontologies with RNF169: view

No data available for Pathways by source for RNF169 Gene

Transcripts for RNF169 Gene

mRNA/cDNA for RNF169 Gene

Unigene Clusters for RNF169 Gene

Ring finger protein 169:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RNF169 Gene

No ASD Table

Relevant External Links for RNF169 Gene

GeneLoc Exon Structure for
RNF169
ECgene alternative splicing isoforms for
RNF169

Expression for RNF169 Gene

mRNA expression in normal human tissues for RNF169 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RNF169 Gene

SOURCE GeneReport for Unigene cluster for RNF169 Gene Hs.745050

genes like me logo Genes that share expressions with RNF169: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RNF169 Gene

Orthologs for RNF169 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RNF169 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF169 35
  • 99.58 (n)
  • 99.44 (a)
RNF169 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RNF169 35
  • 88.25 (n)
  • 87.36 (a)
RNF169 36
  • 87 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF169 35
  • 88.04 (n)
  • 86.3 (a)
RNF169 36
  • 86 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf169 35
  • 82.42 (n)
  • 79.83 (a)
Rnf169 16
Rnf169 36
  • 80 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RNF169 36
  • 59 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RNF169 36
  • 74 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf169 35
  • 83.05 (n)
  • 79.68 (a)
chicken
(Gallus gallus)
Aves RNF169 35
  • 67.24 (n)
  • 65.32 (a)
RNF169 36
  • 62 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RNF169 36
  • 50 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100493580 35
  • 57.47 (n)
  • 51.79 (a)
zebrafish
(Danio rerio)
Actinopterygii rnf169 35
  • 56.84 (n)
  • 51.65 (a)
rnf169 36
  • 35 (a)
OneToOne
Species with no ortholog for RNF169:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RNF169 Gene

ENSEMBL:
Gene Tree for RNF169 (if available)
TreeFam:
Gene Tree for RNF169 (if available)

Paralogs for RNF169 Gene

No data available for Paralogs for RNF169 Gene

Variants for RNF169 Gene

Sequence variations from dbSNP and Humsavar for RNF169 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs113187 -- 74,792,123(+) ATCTT(C/T)GTGCT intron-variant
rs471462 -- 74,804,425(+) gtgct(C/T)ggtga intron-variant
rs472038 -- 74,816,098(-) TATTT(C/G)CTAAC intron-variant
rs474431 -- 74,764,711(+) AGCCA(A/G)TGTTG intron-variant
rs474445 -- 74,818,491(+) GAGCT(C/T)AGATG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF169 Gene

Variant ID Type Subtype PubMed ID
dgv397e1 CNV Complex 17122850
esv34979 CNV Gain 17911159
nsv825994 CNV Gain 20364138
dgv1223n71 CNV Gain 21882294
nsv38718 CNV Loss 16902084
esv2669092 CNV Deletion 23128226
esv2484083 CNV Deletion 19546169
esv2305041 CNV Deletion 18987734
nsv8839 CNV Gain 18304495
esv2744779 CNV Deletion 23290073
dgv5e197 CNV Deletion 19546169

Relevant External Links for RNF169 Gene

HapMap Linkage Disequilibrium report
RNF169

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF169 Gene

Disorders for RNF169 Gene

No disorders were found for RNF169 Gene.

No data available for UniProtKB/Swiss-Prot for RNF169 Gene

Publications for RNF169 Gene

  1. Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method. (PMID: 12056414) Ohara O. … Koseki H. (DNA Res. 2002) 2 3 4
  2. Ring finger protein RNF169 antagonises the ubiquitin-dependent signaling cascade at sites of DNA Damage. (PMID: 22733822) Chen J. … Huen M.S. (J. Biol. Chem. 2012) 3 4
  3. Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks. (PMID: 22742833) Panier S. … Durocher D. (Mol. Cell 2012) 3 4
  4. Human RNF169 is a negative regulator of the ubiquitin-dependent response to DNA double-strand breaks. (PMID: 22492721) Poulsen M. … Mailand N. (J. Cell Biol. 2012) 3 4
  5. Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. (PMID: 22505724) Udeshi N.D. … Carr S.A. (Mol. Cell Proteomics 2012) 3

Products for RNF169 Gene

Sources for RNF169 Gene

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