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Aliases for RNF169 Gene

Aliases for RNF169 Gene

  • Ring Finger Protein 169 2 3
  • RING Finger Protein 169 4 5
  • EC 3.4.24.57 61
  • EC 3.1.4.16 61
  • EC 6.3.2.- 4
  • KIAA1991 4

External Ids for RNF169 Gene

Previous GeneCards Identifiers for RNF169 Gene

  • GC11P074138
  • GC11P074459
  • GC11P070756

Summaries for RNF169 Gene

GeneCards Summary for RNF169 Gene

RNF169 (Ring Finger Protein 169) is a Protein Coding gene. GO annotations related to this gene include ligase activity and nucleosome binding.

UniProtKB/Swiss-Prot for RNF169 Gene

  • Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF169 Gene

Genomics for RNF169 Gene

Regulatory Elements for RNF169 Gene

Enhancers for RNF169 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH11F073786 0.2 ENCODE 45.1 -960.5 -960495 3.2 CREB3L1 FEZF1 DMAP1 YY1 SLC30A9 ZNF143 SP3 NFYC TBX21 MEF2D ENSG00000206913 RNF169 POLD3 RAB6A RPS12P22 MRPL48 ARPC3P4 ENSG00000257038 ENSG00000256034 LOC101928580
GH11F074459 0.8 FANTOM5 ENCODE 27.6 -287.1 -287060 4.1 HDGF PKNOX1 ARNT MLX ARID4B SIN3A DMAP1 YBX1 YY1 ZNF143 RNF169 ENSG00000206913 KCNE3 POLD3 COA4 ENSG00000254974 SPCS2 C2CD3 ENSG00000254928
GH11F075385 1 Ensembl ENCODE 26.2 +638.5 638523 4.0 HDGF ATF1 PKNOX1 ARNT SIN3A ZNF48 YY1 GTF3C2 ZNF121 GATA2 RNF169 ARRB1 KLHL35 POLD3 MIR326 GDPD5 RN7SL239P LOC105369391 RPS3
GH11F073778 1 FANTOM5 Ensembl ENCODE 26.2 -968.9 -968880 2.7 HDGF PKNOX1 ARNT CREB3L1 WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 ENSG00000206913 RNF169 MRPL48 ENSG00000257038 POLD3 RAB6A COA4 UCP2 FAM168A
GH11F074169 1.1 ENCODE 24 -577.4 -577396 3.5 HDGF PKNOX1 ARNT CREB3L1 MLX ARID4B SIN3A YBX1 DMAP1 YY1 ENSG00000206913 RNF169 P4HA3 PPME1 ENSG00000257038 POLD3 COA4 PAAF1 RPS12P22 C2CD3
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around RNF169 on UCSC Golden Path with GeneCards custom track

Promoters for RNF169 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000565395 645 2201 HDGF PKNOX1 ARNT CREB3L1 WRNIP1 ZFP64 ARID4B SIN3A YBX1 DMAP1

Genomic Location for RNF169 Gene

Chromosome:
11
Start:
74,748,855 bp from pter
End:
74,842,413 bp from pter
Size:
93,559 bases
Orientation:
Plus strand

Genomic View for RNF169 Gene

Genes around RNF169 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF169 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF169 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF169 Gene

Proteins for RNF169 Gene

  • Protein details for RNF169 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8NCN4-RN169_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF169
    Protein Accession:
    Q8NCN4
    Secondary Accessions:
    • Q6N015

    Protein attributes for RNF169 Gene

    Size:
    708 amino acids
    Molecular mass:
    77194 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=BAC02700.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for RNF169 Gene

Selected DME Specific Peptides for RNF169 Gene

Q8NCN4:
  • CDETAAA
  • KLQQEEE

Post-translational modifications for RNF169 Gene

  • Ubiquitination at Lys 691
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF169 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for RNF169 Gene

Gene Families for RNF169 Gene

Protein Domains for RNF169 Gene

Suggested Antigen Peptide Sequences for RNF169 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8NCN4

UniProtKB/Swiss-Prot:

RN169_HUMAN :
  • The MIU motif (motif interacting with ubiquitin) mediates the interaction with both Lys-48- and Lys-63-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
  • Belongs to the RNF169 family.
  • Contains 1 RING-type zinc finger.
Domain:
  • The MIU motif (motif interacting with ubiquitin) mediates the interaction with both Lys-48- and Lys-63-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction with ubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs (MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833).
Family:
  • Belongs to the RNF169 family.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RNF169: view

Function for RNF169 Gene

Molecular function for RNF169 Gene

UniProtKB/Swiss-Prot Function:
Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.

Enzyme Numbers (IUBMB) for RNF169 Gene

Gene Ontology (GO) - Molecular Function for RNF169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0031491 nucleosome binding IDA 22742833
GO:0046872 metal ion binding IEA --
GO:0070530 K63-linked polyubiquitin binding IDA 22492721
genes like me logo Genes that share ontologies with RNF169: view
genes like me logo Genes that share phenotypes with RNF169: view

Animal Models for RNF169 Gene

MGI Knock Outs for RNF169:

Animal Model Products

miRNA for RNF169 Gene

miRTarBase miRNAs that target RNF169

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RNF169 Gene

Localization for RNF169 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF169 Gene

Nucleus, nucleoplasm. Note=Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF169 Gene COMPARTMENTS Subcellular localization image for RNF169 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for RNF169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 22733822
GO:0005654 nucleoplasm IEA,IDA 22492721
GO:0035861 site of double-strand break IDA 22492721
genes like me logo Genes that share ontologies with RNF169: view

Pathways & Interactions for RNF169 Gene

SuperPathways for RNF169 Gene

No Data Available

UniProtKB/Swiss-Prot Q8NCN4-RN169_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006974 cellular response to DNA damage stimulus IMP 22492721
GO:0016567 protein ubiquitination IEA --
GO:2000780 negative regulation of double-strand break repair IMP 22492721
genes like me logo Genes that share ontologies with RNF169: view

No data available for Pathways by source and SIGNOR curated interactions for RNF169 Gene

Transcripts for RNF169 Gene

mRNA/cDNA for RNF169 Gene

Unigene Clusters for RNF169 Gene

Ring finger protein 169:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF169 Gene

No ASD Table

Relevant External Links for RNF169 Gene

GeneLoc Exon Structure for
RNF169
ECgene alternative splicing isoforms for
RNF169

Expression for RNF169 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF169 Gene

Protein differential expression in normal tissues from HIPED for RNF169 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (16.6), NK cells (14.0), Blymphocyte (11.8), and CD8 Tcells (11.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for RNF169 Gene



Protein tissue co-expression partners for RNF169 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RNF169 Gene:

RNF169

SOURCE GeneReport for Unigene cluster for RNF169 Gene:

Hs.745050
genes like me logo Genes that share expression patterns with RNF169: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RNF169 Gene

Orthologs for RNF169 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RNF169 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF169 34 35
  • 99.58 (n)
cow
(Bos Taurus)
Mammalia RNF169 34 35
  • 88.25 (n)
dog
(Canis familiaris)
Mammalia RNF169 34 35
  • 88.04 (n)
rat
(Rattus norvegicus)
Mammalia Rnf169 34
  • 83.05 (n)
mouse
(Mus musculus)
Mammalia Rnf169 34 16 35
  • 82.42 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia RNF169 35
  • 74 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RNF169 35
  • 59 (a)
OneToOne
chicken
(Gallus gallus)
Aves RNF169 34 35
  • 67.24 (n)
lizard
(Anolis carolinensis)
Reptilia RNF169 35
  • 50 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100493580 34
  • 57.47 (n)
zebrafish
(Danio rerio)
Actinopterygii rnf169 34 35
  • 56.84 (n)
Species where no ortholog for RNF169 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RNF169 Gene

ENSEMBL:
Gene Tree for RNF169 (if available)
TreeFam:
Gene Tree for RNF169 (if available)

Paralogs for RNF169 Gene

No data available for Paralogs for RNF169 Gene

Variants for RNF169 Gene

Sequence variations from dbSNP and Humsavar for RNF169 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type
rs10437739 -- 74,785,316(+) atatg(A/T)tatat intron-variant
rs10501419 -- 74,789,203(-) AGGTA(A/G)AAGAT intron-variant
rs10501420 -- 74,784,992(-) CTAAC(A/G)TTTAA intron-variant
rs10707469 -- 74,840,807(+) TTTTT(-/T)AACCA intron-variant, downstream-variant-500B, utr-variant-3-prime
rs10899043 -- 74,758,751(+) CGCCC(A/G)CCTTG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF169 Gene

Variant ID Type Subtype PubMed ID
dgv209e214 CNV gain 21293372
dgv5e197 CNV deletion 19546169
dgv676n106 CNV deletion 24896259
esv2305041 CNV deletion 18987734
esv2484083 CNV deletion 19546169
esv2669092 CNV deletion 23128226
esv2744779 CNV deletion 23290073
esv2759837 CNV gain 17122850
esv34979 CNV gain 17911159
esv3548008 CNV deletion 23714750
esv3626846 CNV gain 21293372
nsv1122644 CNV deletion 24896259
nsv1124643 CNV duplication 24896259
nsv1127209 CNV deletion 24896259
nsv38718 CNV deletion 16902084
nsv825994 CNV gain 20364138
nsv8839 CNV gain 18304495
nsv957195 CNV deletion 24416366

Variation tolerance for RNF169 Gene

Residual Variation Intolerance Score: 22.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.76; 57.88% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RNF169 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF169

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF169 Gene

Disorders for RNF169 Gene

Relevant External Links for RNF169

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RNF169

No disorders were found for RNF169 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF169 Gene

Publications for RNF169 Gene

  1. Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method. (PMID: 12056414) Ohara O. … Koseki H. (DNA Res. 2002) 2 3 4 64
  2. Human RNF169 is a negative regulator of the ubiquitin-dependent response to DNA double-strand breaks. (PMID: 22492721) Poulsen M. … Mailand N. (J. Cell Biol. 2012) 3 4 64
  3. Ring finger protein RNF169 antagonises the ubiquitin-dependent signaling cascade at sites of DNA Damage. (PMID: 22733822) Chen J. … Huen M.S. (J. Biol. Chem. 2012) 3 4 64
  4. Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks. (PMID: 22742833) Panier S. … Durocher D. (Mol. Cell 2012) 3 4 64
  5. Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. (PMID: 25281560) Lambert J.P. … Gingras A.C. (J Proteomics 2015) 3 64

Products for RNF169 Gene

Sources for RNF169 Gene

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