RNF169 Gene
protein-coding GIFtS: 41
GCID: GC11P074459
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ring finger protein 169
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Aliases
for RNF169 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Ring Finger Protein 1691 2 3 | | KIAA19911 3 | | E3 Ubiquitin-Protein Ligase RNF1692 | | EC 6.3.2.-3 | | EC 3.1.4.168 | | EC 3.4.24.578 |
Export aliases for RNF169 gene to outside databasesPrevious GC identifers: GC11P074138 GC11P070756 |
Summaries
for RNF169 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: RN169_HUMAN, Q8NCN4Function: Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repairfollowing DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 andcompetes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulatorof DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair
|
Genomic Views
for RNF169 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000011.9 NC_018922.1 NT_167190.1
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the RNF169 gene promoter:
c-Fos Egr-3 AhR AP-1 FosB FOXO3 Roaz FOXO3b FOXO3a c-Jun
Other transcription factors
Search SABiosciences Chromatin IP Primers for RNF169
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNF169 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 11q13.4 Ensembl cytogenetic band: 11q13.4 HGNC cytogenetic band: 11q13.4RNF169 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11P074459: view genomic region
(about GC identifiers)
Start:
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74,459,913 bp from pter |
End:
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74,553,458 bp from pter |
Size:
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93,546 bases |
Orientation:
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plus strand |
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Proteins
for RNF169 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RN169_HUMAN, Q8NCN4 (See
protein sequence)Recommended Name: E3 ubiquitin-protein ligase RNF169 Size: 708 amino acids; 77194 Da
Subcellular location: Nucleus, nucleoplasm. Note=Localizes to sites of double-strand breaks (DSBs) following DNAdamage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products
Sequence caution: Sequence=BAC02700.1; Type=Erroneous initiation;
Secondary accessions: Q6N015Explore the universe of human proteins at neXtProt for RNF169: NX_Q8NCN4
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8NCN4
2 DME Specific Peptides for RNF169 (Q8NCN4)
RNF169 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_001092108.1
ENSEMBL proteins: ENSP00000299563 ENSP00000436339
Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
RNF169 for ontologies About GeneDecksing
RNF169 Antibody Products:
Assay Products for RNF169: |
Protein
Domains /
Families
for RNF169 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RNF169 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q8NCN4ProtoNet protein and cluster: Q8NCN4 1 Blocks protein family: IPB001841 Zn-finger
UniProtKB/Swiss-Prot: RN169_HUMAN, Q8NCN4Domain: The MIU motif (motif interacting with ubiquitin) mediates the interaction with both 'Lys-48'- and'Lys-63'-linked ubiquitin chains (PubMed:22733822 and PubMed:22492721). The UMI motif also mediates interaction withubiquitin. The specificity for different types of ubiquitin is mediated by juxtaposition of ubiquitin-binding motifs(MIU and UMI motifs) with LR motifs (LRMs) (PubMed:22742833)Similarity: Belongs to the RNF169 familySimilarity: Contains 1 RING-type zinc finger |
Function
for RNF169 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RN169_HUMAN, Q8NCN4Function: Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repairfollowing DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 andcompetes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulatorof DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repairEnzyme Numbers (IUBMB): EC 6.3.2.-1 EC 3.4.24.572 EC 3.1.4.162
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF169 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF169
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF169 |
Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table
RNF169 for ontologies About GeneDecksing
2 GenomeRNAi human phenotypes for RNF169:
Animal Models:
3 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Rnf169):
RNF169 for phenotypes About GeneDecksing
|
Pathways & Interactions
for RNF169 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: RN169_HUMAN, Q8NCN4Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RNF169
STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)
5/35 Interacting proteins for RNF169 (Q8NCN42 ENSP000002995634) via UniProtKB, MINT, STRING, and/or I2D (see all 35) About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006974 | response to DNA damage stimulus |
IMP | -- | | GO:0016567 | protein ubiquitination |
IEA | -- | | GO:2000780 | negative regulation of double-strand break repair |
IMP | -- |
RNF169 for ontologies About GeneDecksing
|
Drugs & Compounds
for RNF169 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for RNF169
Search CenterWatch for drugs/clinical trials and news about RNF169 / RN169
|
Transcripts
for RNF169 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for RNF169 gene: NM_001098638.1 Unigene Cluster for RNF169: Ring finger protein 169 Hs.370145 [show with all ESTs]Unigene Representative Sequence: NM_0010986382 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000299563(uc001ovl.4) ENST00000527301
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF169 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF169
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: RNF169 (NM_001098638) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RNF169 | | | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat RNF169 |
Additional cDNA sequence: AB082522.1 BX640750.1 3 DOTS entries: DT.443902 DT.95218489 DT.120758595 24/120 AceView cDNA sequences (see all 120): BE047843 BE858336 BG679092 AW026154 AI589084 AA748391 AA720793 CB147654 BU735651 AI061064 AI082193 BI862829 BX102462 AA437401 BF439964 AI468437 BU735906 BU158897 AI911676 AU253596 AA029162 AI369062 BX640750 BX648162
GeneLoc Exon Structure
|
Expression
for RNF169 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RNF169 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See RNF169 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RNF169
SOURCE GeneReport for Unigene cluster: Hs.370145
SABiosciences Custom PCR Arrays for RNF169
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RNF169
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat RNF169 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNF169 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNF169 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF169 |
Orthologs
for RNF169 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for RNF169 gene from 4/16 species (see all 16) About this table
ENSEMBL Gene Tree for RNF169 (if available)
TreeFam Gene Tree for RNF169 (if available) |
Paralogs
for RNF169 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for RNF169 gene
- RNF1682
RNF169 for paralogs About GeneDecksing
|
Genomic Variants
for RNF169 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 11 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for RNF169 (74459913 - 74553458 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 7 variations for RNF169
4 CNVs: 2932 3858 5634 8692
3 Indels: 44934 101263 33240 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RNF169 |
|
Disorders
/ Diseases
for RNF169 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
| -- |
Publications
for RNF169 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RNF169 gene, integrated from 9 sources (see all 20):
(articles sorted by number of sources associating them with RNF169) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method. (PubMed id 12056414)1, 2, 3 Ohara O.... Koseki H. (2002)
- Tandem protein interaction modules organize the ubiqui tin-dependent response to DNA double-strand breaks. (PubMed id 22742833)1, 2 Panier S....Durocher D. (2012)
- Human RNF169 is a negative regulator of the ubiquitin-dependent response to DNA double-strand breaks. (PubMed id 22492721)1, 2 Poulsen M.... Mailand N. (2012)
- Ring finger protein RNF169 antagonizes the ubiquitin-d ependent signaling cascade at sites of DNA damage. (PubMed id 22733822)1, 2 Chen J....Huen M.S. (2012)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
- Comparative proteomic analysis identifies a role for S UMO in protein quality control. (PubMed id 21693764)1 Tatham M.H....Hay R.T. (2011)
- Purification and identification of endogenous polySUMO conjugates. (PubMed id 21252943)1 Bruderer R....Hay R.T. (2011)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
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External Searches for RNF169 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing RNF169 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RNF169 gene
(According to HUGE)
About This Section
| |
Specialized Databases showing RNF169 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for RNF169 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for RNF169 gene:
Search GeneIP for patents involving RNF169
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for RNF169 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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 | | | RNF169 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF169 |
|  |  |  | | | | Search ThermoFisher Antibodies for RNF169 |
| | | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat RNF169 |
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