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RNF165 Gene

protein-coding   GIFtS: 46
GCID: GC18P043914

Ring Finger Protein 165

  Search for RNF165
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Ring Finger Protein 1651 2
ARKL22
RNF111L22

External Ids:    HGNC: 316961   Entrez Gene: 4944702   Ensembl: ENSG000001416227   UniProtKB: Q6ZSG13   

Export aliases for RNF165 gene to outside databases

Previous GC identifers: GC18U900221 GC18P042169 GC18P040771


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for RNF165 Gene:
Encoded in regions involved in pericentric inversions in patients with bipolar affective disorder. (provided by
RefSeq, Jul 2008)

GeneCards Summary for RNF165 Gene:
RNF165 (ring finger protein 165) is a protein-coding gene. An important paralog of this gene is RNF111.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000018.9  NT_010966.15  NC_018929.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the RNF165 gene promoter:
         Sox5   NF-1   NCX/Ncx   HTF   NF-AT   GATA-2   SRY   NF-AT3   Chx10   NF-AT1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRNF165 promoter sequence
   Search Chromatin IP Primers for RNF165

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RNF165


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 18q21.1   Ensembl cytogenetic band:  18q21.1   HGNC cytogenetic band: 18q21.1

RNF165 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RNF165 gene location

GeneLoc information about chromosome 18         GeneLoc Exon Structure

GeneLoc location for GC18P043914:  view genomic region     (about GC identifiers)

Start:
43,906,772 bp from pter      End:
44,043,103 bp from pter
Size:
136,332 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: RN165_HUMAN, Q6ZSG1 (See protein sequence)
Recommended Name: RING finger protein 165  
Size: 346 amino acids; 39526 Da
Secondary accessions: B3KVD1
Alternative splicing: 2 isoforms:  Q6ZSG1-1   Q6ZSG1-2   

Explore the universe of human proteins at neXtProt for RNF165: NX_Q6ZSG1

Explore proteomics data for RNF165 at MOPED

Post-translational modifications: 

  • Glycosylation2 at Asn199

  • See RNF165 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001243687.1  NP_689683.2  

    ENSEMBL proteins: 
     ENSP00000467730   ENSP00000466208   ENSP00000466809   ENSP00000467609   ENSP00000468365  
     ENSP00000269439   ENSP00000444285  

    RNF165 Human Recombinant Protein Products:

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    Cloud-Clone Corp. ELISAs for RNF165
    Cloud-Clone Corp. CLIAs for RNF165
    Search eBioscience for ELISAs for RNF165 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    RNF: RING-type (C3HC4) zinc fingers

    2 InterPro protein domains:
     IPR001841 Znf_RING
     IPR013083 Znf_RING/FYVE/PHD

    Graphical View of Domain Structure for InterPro Entry Q6ZSG1

    ProtoNet protein and cluster: Q6ZSG1

    1 Blocks protein domain: IPB001841 Zn-finger

    UniProtKB/Swiss-Prot: RN165_HUMAN, Q6ZSG1
    Similarity: Contains 1 RING-type zinc finger


    Find genes that share domains with RNF165           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 3 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding ----
    GO:0008270zinc ion binding IEA--
    GO:0046872metal ion binding ----
         
    Find genes that share ontologies with RNF165           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for RNF165:
     Increased gamma-H2AX phosphory 

         9 MGI mutant phenotypes (inferred from 1 allele(MGI details for Rnf165):
     behavior/neurological  cellular  growth/size/body  homeostasis/metabolism  limbs/digits/tail 
     mortality/aging  muscle  nervous system  skeleton 

    Find genes that share phenotypes with RNF165           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for RNF165
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for RNF165

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for RNF165
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for RNF165

    miRNA
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    Block miRNA regulation of human, mouse, rat RNF165 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate RNF165 (see all 172):
    hsa-miR-1321 hsa-miR-631 hsa-miR-1914* hsa-miR-3921 hsa-miR-3162-5p hsa-miR-877* hsa-miR-298 hsa-miR-3649
    SwitchGear 3'UTR luciferase reporter plasmids (see all 2): RNF165 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat RNF165

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    GenScript: all cDNA clones in your preferred vector: RNF165 (NM_152470)
    Browse Sino Biological Human cDNA Clones
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF165


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    endoplasmic reticulum2
    cytosol1
    extracellular1
    golgi apparatus1
    mitochondrion1
    nucleus1
    plasma membrane1

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for RNF165
    Interactions:

        Search GeneGlobe Interaction Network for RNF165

    STRING Interaction Network Preview (showing 5 interactants - click image to see 16)

    Selected Interacting proteins for RNF165 (Q6ZSG13 ENSP000002694394) via UniProtKB, MINT, STRING, and/or I2D (see all 17)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBE2D2P628373, ENSP000003817174I2D: score=2 STRING: ENSP00000381717
    UBE2D3P610773, ENSP000003497224I2D: score=2 STRING: ENSP00000349722
    UBE2NP610883, ENSP000003161764I2D: score=2 STRING: ENSP00000316176
    UBE2WQ96B023, ENSP000003974534I2D: score=2 STRING: ENSP00000397453
    UBE2D1P516683, ENSP000003630194I2D: score=2 STRING: ENSP00000363019
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for RNF165 (RN165)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for RNF165 gene (2 alternative transcripts): 
    NM_001256758.1  NM_152470.2  

    Unigene Cluster for RNF165:

    Ring finger protein 165
    Hs.501114  [show with all ESTs]
    Unigene Representative Sequence: NM_152470
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000593230 ENST00000587853 ENST00000590330 ENST00000588679 ENST00000586604
    ENST00000269439(uc002lby.1 uc002lcb.1 uc010dnn.1) ENST00000592777
    ENST00000543885(uc002lca.1)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat RNF165 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate RNF165 (see all 172):
    hsa-miR-1321 hsa-miR-631 hsa-miR-1914* hsa-miR-3921 hsa-miR-3162-5p hsa-miR-877* hsa-miR-298 hsa-miR-3649
    SwitchGear 3'UTR luciferase reporter plasmids (see all 2): RNF165 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat RNF165
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    GenScript: all cDNA clones in your preferred vector: RNF165 (NM_152470)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RNF165
    Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat RNF165
    Primer
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    OriGene qPCR primer pairs and template standards for RNF165
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      QuantiTect SYBR Green Assays in human, mouse, rat RNF165
      QuantiFast Probe-based Assays in human, mouse, rat RNF165

    Additional mRNA sequence: 

    AK055997.1 AK074561.1 AK096648.1 AK122819.1 AK123883.1 AK127467.1 BC012190.1 NR_046357.1 

    5 DOTS entries:

    DT.97803328  DT.80102033  DT.91724792  DT.100017417  DT.100017416 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    RNF165 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: ACGACATTGT
    RNF165 Expression
    About this image


    RNF165 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Bone (Muscoskeletal System)    fully expand to see all 2 entries
             Mesenchymal Condensate Cells Zeugopod
             Zeugopod Epiphyseal End
     
     Endothelium (Cardiovascular System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
     
     Paraxial Mesoderm (Gastrulation Derivatives)
             PureStem SK11, NCr-fac & Meso-prx Progenitor
     
     Neural Crest (Gastrulation Derivatives)
             PureStem SK11, NCr-fac & Meso-prx Progenitor
    RNF165 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RNF165 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.501114
        Custom PCR Arrays for RNF165
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF165

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for RNF165 gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rnf1651 , 5 ring finger protein 1651, 5 94.03(n)1
    98.84(a)1
      18 (52.28 cM)5
    2257431  NM_001164504.11  NP_001157976.11 
     774561105 
    chicken
    (Gallus gallus)
    Aves RNF1651 ring finger protein 165 82.46(n)
    88.66(a)
      416382  XM_414696.4  XP_414696.4 
    lizard
    (Anolis carolinensis)
    Reptilia RNF1656
    ring finger protein 165
    86(a)
    1 ↔ 1
    1(34827093-34963825)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia rnf1651 ring finger protein 165 79.51(n)
    86.24(a)
      100487951  XM_002936513.2  XP_002936559.2 
    zebrafish
    (Danio rerio)
    Actinopterygii rnf165b1 ring finger protein 165b 69.89(n)
    72.73(a)
      572413  XM_696125.5  XP_701217.3 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG69236
    --
    5(a)
    1 → many
    3R(7570205-7575751)
    worm
    (Caenorhabditis elegans)
    Secernentea toe-46
    Protein TOE-4 (toe-4) mRNA, complete cds
    12(a)
    1 → many
    I(12738087-12741149) WBGene00012194


    ENSEMBL Gene Tree for RNF165 (if available)
    TreeFam Gene Tree for RNF165 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for RNF165 gene
    RNF1112  
    1 SIMAP similar gene for RNF165 using alignment to 5 protein entries:     RN165_HUMAN (see all proteins):
    RNF111

    Find genes that share paralogs with RNF165           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RNF165 (see all 2783)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 18 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs751881921,2
    F--43912233(+) TGGCCA/GTCTTC 3 -- us2k10--------
    rs1402904951,2
    --43912258(+) TGGGGG/TCAGCC 3 -- us2k10--------
    rs1924388461,2
    --43912286(+) AGAGAA/GCTCAG 3 -- us2k10--------
    rs1451991111,2
    --43912343(+) GGTTCA/TGCCCT 3 -- us2k10--------
    rs1378903311,2
    C--43912349(+) GCCCTA/TGCCAC 3 -- us2k10--------
    rs1820068131,2
    --43912375(+) TGTCTA/GGGTAA 3 -- us2k10--------
    rs1407009591,2
    --43912525(+) GCTGGA/GTGCCC 3 -- us2k10--------
    rs99521131,2
    C,F--43912764(+) CCAGCT/CCCACA 3 -- us2k111Minor allele frequency- C:0.28NA WA EA 508
    rs1172863451,2
    C,F--43912859(+) AATGTC/TCTCTG 3 -- us2k11Minor allele frequency- T:0.01NA 120
    rs769007481,2
    F--43912861(+) TGTCCT/CCTGTC 3 -- us2k11Minor allele frequency- C:0.02EA 120

    HapMap Linkage Disequilibrium report for RNF165 (43906772 - 44043103 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 6 variations for RNF165:    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv596e199CNV Deletion23128226
    esv2677564CNV Deletion23128226
    nsv2279CNV Insertion18451855
    esv24686CNV Loss19812545
    esv22561CNV Loss19812545
    nsv828224CNV Gain20364138

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing RNF165
    DNA2.0 Custom Variant and Variant Library Synthesis for RNF165

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    Find genes that share disorders with RNF165           About GenesLikeMe

    Genetic Association Database (GAD): RNF165
    Human Genome Epidemiology (HuGE) Navigator: RNF165 (1 document)

    Export disorders for RNF165 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RNF165 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with RNF165)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    3. A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. (PubMed id 19690564)1 van Wijk S.J....Timmers H.T. (Mol. Syst. Biol. 2009)
    4. Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. (PubMed id 19159218)2 Chen R.... Zou H. (J. Proteome Res. 2009)
    5. Genome-wide association to body mass index and waist circumference: the Framingham Heart Study 100K project. (PubMed id 17903300)4 Fox C.S....Atwood L.D. (BMC Med. Genet. 2007)
    6. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
    7. DNA sequence and analysis of human chromosome 18. (PubMed id 16177791)2 Nusbaum C.... Lander E.S. (Nature 2005)
    8. Candidate psychiatric illness genes identified in patients with pericentric inversions of chromosome 18. (PubMed id 15722956)1 Pickard B.S.... Muir W.J. (Psychiatr. Genet. 2005)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    10. Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)1 Bonaldo M.F.... Soares M.B. (Genome Res. 1996)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 494470 HGNC: 31696 Ensembl:ENSG00000141622 euGenes: HUgn494470 ECgene: RNF165
    H-InvDB: RNF165

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for RNF165 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RNF165 gene:
    Search GeneIP for patents involving RNF165

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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