RNF149 Gene
protein-coding GIFtS: 47
GCID: GC02M101887
|
|
ring finger protein 149
| |
Aliases
for RNF149 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Ring Finger Protein 1491 2 3 | | DNAPTP22 3 | | DNA Polymerase-Transactivated Protein 22 3 | | FLJ905041 | | E3 Ubiquitin-Protein Ligase RNF1492 | | EC 6.3.2.-3 |
Export aliases for RNF149 gene to outside databasesPrevious GC identifers: GC02M101513 GC02M101351 GC02M101258 GC02M095656 |
Summaries
for RNF149 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: RN149_HUMAN, Q8NC42Function: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation
|
Genomic Views
for RNF149 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000002.11 NC_018913.1 NT_022171.15
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the RNF149 gene promoter:
RFX1 HTF AP-4 LCR-F1 POU3F2 HEN1 CP2 POU2F1 POU2F1a Cart-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for RNF149
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNF149 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 2q11.2 Ensembl cytogenetic band: 2q11.2 HGNC cytogenetic band: 2q12.1RNF149 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 2 GeneLoc Exon Structure GeneLoc location for GC02M101887: view genomic region
(about GC identifiers)
Start:
|
101,887,681 bp from pter |
End:
|
101,925,178 bp from pter |
Size:
|
37,498 bases |
Orientation:
|
minus strand |
|
Proteins
for RNF149 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RN149_HUMAN, Q8NC42 (See
protein sequence)Recommended Name: E3 ubiquitin-protein ligase RNF149 precursor Size: 400 amino acids; 43165 Da
Subcellular location: Membrane; Single-pass membrane protein (Potential)
Secondary accessions: Q53S14 Q8N5I8 Q8NBY5 Q8WUU3Explore the universe of human proteins at neXtProt for RNF149: NX_Q8NC42
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8NC42
RNF149 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_775918.2
ENSEMBL proteins: ENSP00000399090 ENSP00000295317
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016021 | integral to membrane |
IEA | -- |
RNF149 for ontologies About GeneDecksing
RNF149 Antibody Products:
Assay Products for RNF149: |
Protein
Domains /
Families
for RNF149 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RNF149 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q8NC42ProtoNet protein and cluster: Q8NC42 1 Blocks protein family: IPB001841 Zn-finger
UniProtKB/Swiss-Prot: RN149_HUMAN, Q8NC42Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity)Similarity: Contains 1 PA (protease associated) domainSimilarity: Contains 1 RING-type zinc finger |
Function
for RNF149 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RN149_HUMAN, Q8NC42Function: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradationEnzyme Number (IUBMB): EC 6.3.2.-1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF149 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF149
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF149 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0008270 | zinc ion binding |
IEA | -- | | GO:0016874 | ligase activity |
IEA | -- |
RNF149 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for RNF149:
|
Pathways & Interactions
for RNF149 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: RN149_HUMAN, Q8NC42Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RNF149
STRING Interaction
Network Preview (showing 4 interactants - click image to see more details)
5/68 Interacting proteins for RNF149 (Q8NC422 ENSP000002953174) via UniProtKB, MINT, STRING, and/or I2D (see all 68) About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016567 | protein ubiquitination |
IEA | -- |
RNF149 for ontologies About GeneDecksing
|
Drugs & Compounds
for RNF149 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for RNF149
Search CenterWatch for drugs/clinical trials and news about RNF149 / RN149
|
Transcripts
for RNF149 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for RNF149 gene: NM_173647.3 Unigene Cluster for RNF149: Ring finger protein 149 Hs.142074 [show with all ESTs]Unigene Representative Sequence: NM_1736476 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000485752 ENST00000424632(uc002tax.2) ENST00000295317(uc002taz.2)
ENST00000490553 ENST00000463726 ENST00000478404
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNF149 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNF149
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: RNF149 (NM_173647) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RNF149 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF149 |
Additional cDNA sequence: AK074985.1 AK075141.1 AK123500.1 AY450390.1 BC019355.1 BC032328.1 BC045743.1 15 DOTS entries: DT.414998 DT.408425 DT.97763360 DT.40221131 DT.414999 DT.439071 DT.86852548 DT.91654828 DT.102828241 DT.100811730 DT.100027144 DT.100720195 DT.95374775 DT.100837766 DT.120942375 24/292 AceView cDNA sequences (see all 292): BQ775527 BM311753 AA902645 CA441783 AK074985 AA629128 BM979209 T40905 AA320132 AI564061 BM978724 AI143497 BF197913 AA989416 AW513379 BE891269 T39949 AI286184 CA430329 AJ705152 CR591001 NM_173647 CF529478 BU633501
GeneLoc Exon Structure
|
Expression
for RNF149 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RNF149 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GGCTTTCCCT
About this image
See RNF149 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RNF149
SOURCE GeneReport for Unigene cluster: Hs.142074
SABiosciences Custom PCR Arrays for RNF149
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RNF149
Browse OriGene validated miRNA SYBR primer pairs
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Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF149 |
Orthologs
for RNF149 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for RNF149 gene from 4/16 species (see all 16) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
RNF1491 |
ring finger protein 149 |
75.33(n)
71.67(a) |
|
418712 XM_416911.3 XP_416911.3 |
lizard
(Anolis carolinensis) |
Reptilia |
RNF1496 |
-- |
60(a) |
1 ↔ 1 |
GL343542.1(107344-119094) |
fruit fly
(Drosophila melanogaster) |
Insecta |
gol6 |
goliath |
19(a) |
1 → many |
2R(20960150-20971843) |
worm
(Caenorhabditis elegans) |
Secernentea |
H10E21.56 |
Protein H10E21.5 |
16(a) |
1 → many |
III(20527-23962) |
ENSEMBL Gene Tree for RNF149 (if available)
TreeFam Gene Tree for RNF149 (if available) |
Paralogs
for RNF149 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for RNF149 gene
- ZNRF32 RNF432 RNF1482 RNF1332 RNF1672 RNF1302 RNF1502 RNF2152
- RNF1282 ZNRF42 RNF132
7 SIMAP similar genes for RNF149 using alignment to 2 protein entries: RN149_HUMAN (see all proteins):LOC284996 RNF150 RNF128 RNF148 RNF130 RNF133
RNF24
RNF149 for paralogs About GeneDecksing
|
Genomic Variants
for RNF149 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 2 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for RNF149 (101887681 - 101925178 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for RNF149
1 CNV: 47994 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RNF149 |
|
Disorders
/ Diseases
for RNF149 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RNF149 for disorders About GeneDecksing
2  diseases for RNF149: About MalaCardspancreatic cancer pancreatitis
Export disorders for RNF149 gene to outside databases
|
Publications
for RNF149 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RNF149 gene, integrated from 9 sources (see all 20):
(articles sorted by number of sources associating them with RNF149) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- RING finger protein 149 is an E3 ubiquitin ligase active on wild-type BRAF. (PubMed id 22628551)1, 2 Hong S.W.... Kim T.W. (2012)
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W....Wilson R.K. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- Genome-wide association study of pancreatic cancer in Japanese population. (PubMed id 20686608)1 Low S.K....Sakamoto H. (2010)
- Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
|
External Searches for RNF149 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing RNF149 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RNF149 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RNF149 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for RNF149 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for RNF149 gene:
Search GeneIP for patents involving RNF149
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for RNF149 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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 | | | RNF149 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNF149 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNF149 |
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