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Aliases for RNF146 Gene

Aliases for RNF146 Gene

  • Ring Finger Protein 146 2 3
  • Dactylidin 3 4
  • Iduna 3 4
  • E3 Ubiquitin-Protein Ligase RNF146 3
  • RING Finger Protein 146 4
  • 2610509H23Rik 3
  • DJ351K20.1 3
  • EC 6.3.2.- 4

External Ids for RNF146 Gene

Previous GeneCards Identifiers for RNF146 Gene

  • GC06P127568
  • GC06P127629
  • GC06P125164
  • GC06P127587

Summaries for RNF146 Gene

GeneCards Summary for RNF146 Gene

RNF146 (Ring Finger Protein 146) is a Protein Coding gene. Diseases associated with RNF146 include cherubism. GO annotations related to this gene include ubiquitin-protein transferase activity and poly-ADP-D-ribose binding.

UniProtKB/Swiss-Prot for RNF146 Gene

  • E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at Lys-48 and Lys-63, while AXIN1 is only ubiquitinated at Lys-48. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.

Gene Wiki entry for RNF146 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF146 Gene

Genomics for RNF146 Gene

Regulatory Elements for RNF146 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RNF146 Gene

Start:
127,266,610 bp from pter
End:
127,288,567 bp from pter
Size:
21,958 bases
Orientation:
Plus strand

Genomic View for RNF146 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RNF146 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF146 Gene

Proteins for RNF146 Gene

  • Protein details for RNF146 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NTX7-RN146_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF146
    Protein Accession:
    Q9NTX7
    Secondary Accessions:
    • E1P572
    • Q6FIB2
    • Q7L8H4
    • Q96K03
    • Q96T06
    • Q9NTX6

    Protein attributes for RNF146 Gene

    Size:
    359 amino acids
    Molecular mass:
    38950 Da
    Quaternary structure:
    • Can form homooligomers. Interacts with PARsylated AXIN1, AXIN2, BLZF1, CASC3, HIST1H1C, IPO7, LIG3, NCL, PARP1, XRCC1, XRCC5 and XRCC6. Interacts with DDB1, DHX15, IQGAP1, LRPPRC, PARP2, PRKDC, RUVBL2, TNKS1 and TNKS2. Binding often leads to interactor ubiquitination, in the presence of the appropriate E1 and E2 enzymes, and proteasomal degradation.
    Miscellaneous:
    • Was named dactylidin after the Greek term daktylidi for ring, the thing around the finger (PubMed:15813938). Was named Iduna after the Norse goddess of protection and eternal youth (PubMed:21602803).

    Three dimensional structures from OCA and Proteopedia for RNF146 Gene

    Alternative splice isoforms for RNF146 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF146 Gene

Proteomics data for RNF146 Gene at MOPED

Post-translational modifications for RNF146 Gene

  • Ubiquitinated; autoubiquitinated. Polyubiquitinated in the presence of UBE2D1, UBE2D2 and UBE2D3. Multimonoubiquitinated in the presence of UBE2E1. Not ubiquitinated in the presence of UBE2H, CDC34, UBE2L3, UBE2L6, nor UBE2C. In the absence of PAR, autoubiquitination occurs on Lys-85, Lys-95 and Lys-176 via Lys-11 and Lys-48 ubiquitin linkages. In the presence of PAR, Lys-131 and Lys-176 are ubiquitinated via Lys-6, Lys-33 and Lys-48 ubiquitin linkages. Autoubiquitination is enhanced upon PAR-binding.
  • Ubiquitination at Lys85, Lys95, Lys131, and Lys176
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for RNF146 Gene

Domains for RNF146 Gene

Gene Families for RNF146 Gene

HGNC:
  • RNF :RING-type (C3HC4) zinc fingers

Protein Domains for RNF146 Gene

Suggested Antigen Peptide Sequences for RNF146 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NTX7

UniProtKB/Swiss-Prot:

RN146_HUMAN :
  • Q9NTX7
Domain:
  • The WWE domain mediates non-covalent PAR-binding.
  • Contains 1 WWE domain.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RNF146: view

Function for RNF146 Gene

Molecular function for RNF146 Gene

UniProtKB/Swiss-Prot Function: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at Lys-48 and Lys-63, while AXIN1 is only ubiquitinated at Lys-48. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.

Enzyme Numbers (IUBMB) for RNF146 Gene

Gene Ontology (GO) - Molecular Function for RNF146 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IDA 21478859
GO:0005515 protein binding IPI 21478859
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0061630 ubiquitin protein ligase activity TAS --
genes like me logo Genes that share ontologies with RNF146: view

Phenotypes for RNF146 Gene

GenomeRNAi human phenotypes for RNF146:
genes like me logo Genes that share phenotypes with RNF146: view

Animal Model Products

CRISPR Products

miRNA for RNF146 Gene

miRTarBase miRNAs that target RNF146

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for RNF146 Gene

Localization for RNF146 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF146 Gene

Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RNF146 Gene COMPARTMENTS Subcellular localization image for RNF146 gene
Compartment Confidence
cytosol 5
nucleus 5
plasma membrane 2

Gene Ontology (GO) - Cellular Components for RNF146 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with RNF146: view

Pathways for RNF146 Gene

SuperPathways for RNF146 Gene

No Data Available

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

UniProtKB/Swiss-Prot Q9NTX7-RN146_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF146 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016055 Wnt signaling pathway IEA --
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process IMP 21478859
GO:0051865 protein autoubiquitination IDA 21478859
GO:0070936 protein K48-linked ubiquitination IMP 21478859
GO:0090263 positive regulation of canonical Wnt signaling pathway TAS --
genes like me logo Genes that share ontologies with RNF146: view

No data available for Pathways by source for RNF146 Gene

Transcripts for RNF146 Gene

Unigene Clusters for RNF146 Gene

Ring finger protein 146:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RNF146

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF146 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c
SP1: - - - - -
SP2: - - -
SP3: - - - -
SP4: - - -
SP5: - - - - - - - -
SP6: - - - - - -
SP7: - - - -
SP8: - - - - - -
SP9: -
SP10: - - - - -
SP11: - - - - - - -
SP12: - -

Relevant External Links for RNF146 Gene

GeneLoc Exon Structure for
RNF146
ECgene alternative splicing isoforms for
RNF146

Expression for RNF146 Gene

mRNA expression in normal human tissues for RNF146 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RNF146 Gene

SOURCE GeneReport for Unigene cluster for RNF146 Gene Hs.267120

mRNA Expression by UniProt/SwissProt for RNF146 Gene

Q9NTX7-RN146_HUMAN
Tissue specificity: Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease
genes like me logo Genes that share expressions with RNF146: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for RNF146 Gene

Orthologs for RNF146 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF146 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF146 35
  • 100 (n)
  • 100 (a)
RNF146 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RNF146A 36
  • 95 (a)
OneToMany
RNF146B 35
  • 92.51 (n)
  • 95.97 (a)
RNF146B 36
  • 96 (a)
OneToMany
dog
(Canis familiaris)
Mammalia RNF146 35
  • 88.3 (n)
  • 91.64 (a)
RNF146 36
  • 92 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf146 35
  • 90.45 (n)
  • 93.54 (a)
Rnf146 16
Rnf146 36
  • 92 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RNF146 36
  • 97 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RNF146 36
  • 80 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf146 35
  • 88.16 (n)
  • 92.05 (a)
chicken
(Gallus gallus)
Aves RNF146 35
  • 80.48 (n)
  • 88.29 (a)
RNF146 36
  • 87 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RNF146 36
  • 82 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.14626 35
tropical clawed frog
(Silurana tropicalis)
Amphibia rnf146 35
  • 68.5 (n)
  • 73.14 (a)
Str.11229 35
zebrafish
(Danio rerio)
Actinopterygii rnf146 35
  • 57.81 (n)
  • 57.01 (a)
rnf146 36
  • 54 (a)
OneToOne
wufi29e08 35
fruit fly
(Drosophila melanogaster)
Insecta CG8786 36
  • 26 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea C02B8.6 36
  • 16 (a)
OneToMany
C26B9.6 36
  • 17 (a)
OneToMany
C55A6.1 36
  • 28 (a)
OneToMany
Y105E8A.14 36
  • 25 (a)
OneToMany
Species with no ortholog for RNF146:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RNF146 Gene

ENSEMBL:
Gene Tree for RNF146 (if available)
TreeFam:
Gene Tree for RNF146 (if available)

Paralogs for RNF146 Gene

No data available for Paralogs for RNF146 Gene

Variants for RNF146 Gene

Sequence variations from dbSNP and Humsavar for RNF146 Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type MAF
rs877102 -- 127,265,941(+) ACTGA(C/T)TTCAG upstream-variant-2KB
rs877661 -- 127,266,770(-) GAGCT(A/C)ACTCC utr-variant-5-prime, upstream-variant-2KB, intron-variant, nc-transcript-variant
rs1013640 -- 127,272,419(+) CCAGC(A/G)TTTAT intron-variant
rs2038378 -- 127,269,255(+) TAAAG(C/T)ATTTA intron-variant
rs2144742 -- 127,274,641(+) TTTTA(A/C)CATTC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RNF146 Gene

Variant ID Type Subtype PubMed ID
nsv7385 OTHER Inversion 18451855

Relevant External Links for RNF146 Gene

HapMap Linkage Disequilibrium report
RNF146

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF146 Gene

Disorders for RNF146 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for RNF146 Gene including...

Search for RNF146 Gene in MalaCards »

UniProtKB/Swiss-Prot

RN146_HUMAN
  • Note=Defects in RNF146 are a cause of susceptibility to breast cancer.

Relevant External Links for RNF146

Genetic Association Database (GAD)
RNF146
Human Genome Epidemiology (HuGE) Navigator
RNF146
genes like me logo Genes that share disorders with RNF146: view

Publications for RNF146 Gene

  1. The novel cytosolic RING finger protein dactylidin is up-regulated in brains of patients with Alzheimer's disease. (PMID: 15813938) von Rotz R.C. … Nitsch R.M. (Eur. J. Neurosci. 2005) 3 4 23
  2. Genome-wide association study provides evidence for a breast cancer risk locus at 6q22.33. (PMID: 18326623) Gold B. … Offit K. (Proc. Natl. Acad. Sci. U.S.A. 2008) 3 4 48
  3. The RNF146 and ECHDC1 genes as candidates for inherited breast and ovarian cancer in Jewish Ashkenazi women. (PMID: 19517271) Menachem T.D. … Friedman E. (Fam. Cancer 2009) 3 4 48
  4. RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling. (PMID: 21478859) Zhang Y. … Cong F. (Nat. Cell Biol. 2011) 3 4
  5. Ubiquitin ligase RNF146 regulates tankyrase and Axin to promote Wnt signaling. (PMID: 21799911) Callow M.G. … Costa M. (PLoS ONE 2011) 3 4

Products for RNF146 Gene

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