RNASEH1 Gene
protein-coding GIFtS: 57
GCID: GC02M003592
|
|
ribonuclease H1
| |
Aliases for RNASEH1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Ribonuclease H11 2 | | Ribonuclease H Type II2 3 | | RNH12 3 | | RNase H12 3 | | H1RNA2 | | EC 3.1.26.43 |
Export aliases for RNASEH1 gene to outside databasesPrevious GC identifers: GC02M003070 GC02M003234 GC02M003157 GC02M003158 GC02M003085 GC02M003570 GC02M003535 |
Summaries for RNASEH1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: RNH1_HUMAN, O60930Function: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids Gene Wiki entry for RNASEH1
|
Genomic Views for RNASEH1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000002.11 NC_018913.1 NT_022139.13
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the RNASEH1 gene promoter: GR GR-beta IRF-1 CUTL1 Evi-1 PPAR-gamma1 E2F-1 HOXA5 GR-alpha PPAR-gamma2 Other transcription factors
Search SABiosciences Chromatin IP Primers for RNASEH1
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RNASEH1 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 2p25 Ensembl cytogenetic band: 2p25.3 HGNC cytogenetic band: 2p25RNASEH1 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 2 GeneLoc Exon Structure GeneLoc location for GC02M003592: view genomic region
(about GC identifiers)
Start:
|
3,592,383 bp from pter |
End:
|
3,606,206 bp from pter |
Size:
|
13,824 bases |
Orientation:
|
minus strand |
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Proteins for RNASEH1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: RNH1_HUMAN, O60930 (See
protein sequence)Recommended Name: Ribonuclease H1 Size: 286 amino acids; 32064 Da
Cofactor: Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substratebinding
Subunit: Monomer (Probable)
Subcellular location: Cytoplasm (Potential)
4 PDB 3D structures from and Proteopedia for RNASEH1:2QK9 (3D)
  2QKB (3D)
  2QKK (3D)
  3BSU (3D)
 
Secondary accessions: B3KQU4 O60523 O60857 Q57Z93 Q5U0C1 Q6FHD4Explore the universe of human proteins at neXtProt for RNASEH1: NX_O60930
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O60930 RNASEH1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_002927.2 ENSEMBL proteins: ENSP00000404926 ENSP00000313350 ENSP00000411646 Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
RNASEH1 for ontologies About GeneDecksing
RNASEH1 Antibody Products: Assay Products for RNASEH1: |
Protein
Domains / Families for RNASEH1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RNASEH1 for domains About GeneDecksing
5 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry O60930ProtoNet protein and cluster: O60930 1 Blocks protein family: IPB002156 RNase H
UniProtKB/Swiss-Prot: RNH1_HUMAN, O60930Similarity: Belongs to the RNase H familySimilarity: Contains 1 RNase H domain |
Function for RNASEH1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: RNH1_HUMAN, O60930Function: Endonuclease that specifically degrades the RNA of RNA-DNA hybridsCatalytic activity: Endonucleolytic cleavage to 5'-phosphomonoesterEnzyme regulation: In the presence of magnesium, manganese is inhibitory Genatlas biochemistry entry for RNASEH1:ribonuclease,RNAse H family,1,ubiquitously expressed,located in the SMCR critical region Enzyme Number (IUBMB): EC 3.1.26.41
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNASEH1 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNASEH1 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript Custom all cDNA clones Services for RNASEH1 | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RNASEH1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNASEH1  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNASEH1 |
Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table
RNASEH1 for ontologies About GeneDecksing
Animal Models: Mouse knock-out Rnaseh1tm1Crh for RNASEH1 4 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Rnaseh1):
RNASEH1 for phenotypes About GeneDecksing
|
Pathways & Interactions for RNASEH1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways  About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Cell cycle Transition and termination of DNA replacation | | | 2 | Lagging Strand Synthesis | |
Pathway sources See GeneCards unified pathways Show all pathways
1 EMD Millipore Pathway for RNASEH1
1 GeneGo (Thomson Reuters) Pathway for RNASEH1
1
Kegg Pathway (Kegg details for RNASEH1):
RNASEH1 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RNASEH1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 9)
 5/9 Interacting proteins for RNASEH1 (O609303 ENSP000003133504) via UniProtKB, MINT, STRING, and/or I2D (see all 9)About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table
RNASEH1 for ontologies About GeneDecksing
|
Drugs & Compounds for RNASEH1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
RNASEH1 for compounds About GeneDecksing
Browse Tocris compounds for RNASEH1
2 HMDB Compounds for RNASEH1 About this table 6 Novoseek chemical compound relationships for RNASEH1 gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| deoxyribonucleotide |
79.9 |
1 |
17028157 (1) |
| oligoribonucleotide |
69.6 |
1 |
14506260 (1) |
| carboxylate |
53.9 |
1 |
17226948 (1) |
| ribose |
44.7 |
2 |
8389211 (2) |
| oligonucleotide |
20 |
1 |
14500841 (1) |
| alanine |
3.92 |
2 |
12473655 (1), 14506260 (1) |
Search CenterWatch for drugs/clinical trials and news about RNASEH1 / RNH1 
|
Transcripts for RNASEH1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for RNASEH1 gene: NM_002936.3 Unigene Cluster for RNASEH1: Ribonuclease H1 Hs.568006 [show with all ESTs]Unigene Representative Sequence: AK0276104 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000436842(uc002qxs.3) ENST00000315212(uc002qxt.3) ENST00000464986 ENST00000454734
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RNASEH1 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RNASEH1 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript Custom all cDNA clones Services for RNASEH1 | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RNASEH1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNASEH1  |
Additional cDNA sequence: AF039652.1 AF048994.1 AF048995.1 AJ224117.1 AK027610.1 AK075490.1 AK096913.1 BC002973.1 BC015873.1 BT019670.1 CR541820.1 10 DOTS entries: DT.454070 DT.100789504 DT.100789507 DT.110932 DT.120943426 DT.92042333 DT.95375436 DT.100735469 DT.121612807 DT.91753140 24/184 AceView cDNA sequences (see all 184): AI246595 BE778327 BM794472 BE265817 BM839989 CR596638 BQ434185 CA775004 CD299961 BQ021574 AW197633 AI201268 BM770862 CR602075 BU657200 BF223130 AA974362 AI225087 AI889735 AA972279 AI279405 CB163780 BM762437 NM_002936 GeneLoc Exon Structure
5/7 Alternative Splicing Database (ASD) splice patterns (SP) for RNASEH1 (see all 7) About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | ^ | 2 | ^ | 3a | · | 3b | ^ | 4a | · | 4b | · | 4c | ^ | 5a | · | 5b | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9a | · | 9b | · | 9c | |
| SP1: | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | - |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
ECgene alternative splicing isoforms for RNASEH1
|
Expression for RNASEH1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RNASEH1 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: GATATACCAC
About this image See RNASEH1 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for RNASEH1
SOURCE GeneReport for Unigene cluster: Hs.568006 UniProtKB/Swiss-Prot: RNH1_HUMAN, O60930Tissue specificity: Ubiquitous SABiosciences Custom PCR Arrays for RNASEH1
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RNASEH1 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RNASEH1 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RNASEH1 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RNASEH1 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNASEH1 |
Orthologs for RNASEH1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the last universal common ancestor (LUCA).
Orthologs for RNASEH1 gene from 9/27 species (see all 27) About this table
ENSEMBL Gene Tree for RNASEH1 (if available) TreeFam Gene Tree for RNASEH1 (if available)  |
Paralogs for RNASEH1 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for RNASEH1 gene
- ENSG000002557672
RNASEH1 for paralogs About GeneDecksing
3 Pseudogenes.org Pseudogenes for RNASEH1 PGOHUM00000236976 PGOHUM00000237240 PGOHUM00000245195
|
Genomic Variants for RNASEH1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 2 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
|---|
HapMap Linkage Disequilibrium report for RNASEH1 (3592383 - 3606206 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 1 variation for RNASEH1 1 CNV: 58980  | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases for RNASEH1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RNASEH1 for disorders About GeneDecksing
OMIM gene information: 604123
OMIM disorders: --
9 diseases for RNASEH1: About MalaCardscartilage-hair hypoplasia swine influenza metaphyseal dysplasia ataxia telangiectasia influenza hepatitis b ataxia hepatitis immunodeficiency 2 diseases from the University of Copenhagen DISEASES database for RNASEH1:cartilage-hair hypoplasia Swine influenza Human Genome Epidemiology (HuGE) Navigator: RNASEH1 (1 document) Export disorders for RNASEH1 gene to outside databases
|
Publications for RNASEH1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RNASEH1 gene, integrated from 9 sources (see all 51): (articles sorted by number of sources associating them with RNASEH1) | |  | Utopia: connect your pdf to the dynamic world of online information |
- Properties of cloned and expressed human RNase H1. (PubMed id 10497183)1, 2, 9 Wu H.... Crooke S.T. (1999)
- Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. (PubMed id 17964265)1, 3, 9 Nowotny M....Yang W. (2007)
- Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI. (PubMed id 9799596)1, 2, 9 Cerritelli S.M. and Crouch R.J. (1998)
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W....Wilson R.K. (2005)
- Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PubMed id 16303743)1, 2 Otsuki T....Isogai T. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome. (PubMed id 12036296)1, 3 ten Asbroek A.L....Baas F. (2002)
- Cloning, subcellular localization and functional expression of human RNase HII. (PubMed id 9894807)1, 2 Frank P....Holzmann K. (1998)
- Molecular cloning and expression of cDNA for human RNase H. (PubMed id 9512096)1, 2 Wu H.... Crooke S.T. (1998)
- Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines. (PubMed id 12473655)1, 9 Lima W.F....Crooke S.T. (2003)
|
External Searches for RNASEH1 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing RNASEH1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RNASEH1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RNASEH1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for RNASEH1 | Pharmacogenomics, SNPs, Pathways | | Wikipedia | http://en.wikipedia.org/wiki/RNase_H |
|
| | |
About This Section
| Patent Information for RNASEH1 gene: Search GeneIP for patents involving RNASEH1
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for RNASEH1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
|
 | |
 | |
 |
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| |  |  |  |  | | | | |
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 |
 | | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RNASEH1 |
|  |  |  | | | Search ThermoFisher Antibodies for RNASEH1 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RNASEH1 |
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