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RLTPR Gene

protein-coding   GIFtS: 44
GCID: GC16P067679

RGD Motif, Leucine Rich Repeats, Tropomodulin Domain And...

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
RGD Motif, Leucine Rich Repeats, Tropomodulin Domain And Proline-Rich
Containing1 2
     Leucine-Rich Repeat1
Leucine Rich Repeat Containing 16C1 2     Tropomodulin And Proline-Rich Containing Protein1
CARMIL2b2 3     CARMIL22
LRRC16C2 3     Leucine-Rich Repeat-Containing Protein 16C2
RGD, Leucine-Rich Repeat, Tropomodulin And Proline-Rich-Containing Protein2 3     RGD, Leucine-Rich Repeat, Tropomodulin And Proline-Rich Containing Protein2
RGD1     

External Ids:    HGNC: 270891   Entrez Gene: 1462062   Ensembl: ENSG000001597537   OMIM: 6108595   UniProtKB: Q6F5E83   

Export aliases for RLTPR gene to outside databases

Previous GC identifers: GC16P066231 GC16P053551


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for RLTPR Gene:
RLTPR (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing) is a protein-coding gene. An important paralog of this gene is LRRC16B.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000016.9  NT_010498.16  NC_018927.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the RLTPR gene promoter:
         COUP-TF1   AhR   Nkx2-5   STAT5A   HNF-4alpha1   GATA-1   CREB   COUP-TF   deltaCREB   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): RLTPR promoter sequence
   Search Chromatin IP Primers for RLTPR

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RLTPR


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q22.1   Ensembl cytogenetic band:  16q22.1   HGNC cytogenetic band: 16q22.1

RLTPR Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RLTPR gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P067679:  view genomic region     (about GC identifiers)

Start:
67,678,822 bp from pter      End:
67,691,472 bp from pter
Size:
12,651 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: LR16C_HUMAN, Q6F5E8 (See protein sequence)
Recommended Name: Leucine-rich repeat-containing protein 16C  
Size: 1435 amino acids; 154689 Da
Developmental stage: Expressed in fetal skin
Sequence caution: Sequence=BAD26751.1; Type=Erroneous initiation;
Secondary accessions: B8X2Z3
Alternative splicing: 2 isoforms:  Q6F5E8-1   Q6F5E8-2   

Explore the universe of human proteins at neXtProt for RLTPR: NX_Q6F5E8

Explore proteomics data for RLTPR at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys563, Lys595, Lys668
  • Modification sites at PhosphoSitePlus

  • See RLTPR Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001013860.1  
    ENSEMBL proteins: 
     ENSP00000334958   ENSP00000473580   ENSP00000441481   ENSP00000473419  

    RLTPR Human Recombinant Protein Products:

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    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for RLTPR

     
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    antibodies-online peptides for RLTPR

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    Cloud-Clone Corp. ELISAs for RLTPR
    Cloud-Clone Corp. CLIAs for RLTPR
    Search eBioscience for ELISAs for RLTPR 
    antibodies-online kits for RLTPR (4 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    ProtoNet protein and cluster: Q6F5E8

    1 Blocks protein domain: IPB001611 Leucine-rich repeat signature

    UniProtKB/Swiss-Prot: LR16C_HUMAN, Q6F5E8
    Similarity: Belongs to the CARMIL family
    Similarity: Contains 16 LRR (leucine-rich) repeats


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for RLTPR

    miRNA
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    3 qRT-PCR Assays for microRNAs that regulate RLTPR:
    hsa-miR-3163 hsa-miR-216b hsa-miR-216a
    Browse SwitchGear 3'UTR luciferase reporter plasmids
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    Browse Sino Biological Human cDNA Clones
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RLTPR

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RLTPR


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    LR16C_HUMAN, Q6F5E8: Cytoplasm. Note=Exhibits a fibrillar pattern reminiscent of intermediate filaments.
    Colocalizes with vimentin
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus3
    cytosol2
    endosome1

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--

    Find genes that share ontologies with RLTPR           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for RLTPR
    Interactions:

        Search GeneGlobe Interaction Network for RLTPR

    STRING Interaction Network Preview (showing 5 interactants - click image to see 10)

    Selected Interacting proteins for RLTPR (ENSP000003349584) via UniProtKB, MINT, STRING, and/or I2D (see all 10)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DCTN1ENSP000003547914STRING: ENSP00000354791
    DCTN2ENSP000004089104STRING: ENSP00000408910
    ACTR10ENSP000002542864STRING: ENSP00000254286
    DCTN4ENSP000004149064STRING: ENSP00000414906
    ACTR1AENSP000003589214STRING: ENSP00000358921
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for RLTPR (LR16C)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for RLTPR gene: 
    NM_001013838.1  

    Unigene Cluster for RLTPR:

    RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing
    Hs.611432  [show with all ESTs]
    Unigene Representative Sequence: AB113647
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000334583(uc010cel.1 uc002etn.3 uc010vjr.2) ENST00000602563
    ENST00000602467 ENST00000545661 ENST00000602742 ENST00000602321 ENST00000602562
    ENST00000602633 ENST00000602931 ENST00000602924 ENST00000602368 ENST00000602705

    miRNA
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    3 qRT-PCR Assays for microRNAs that regulate RLTPR:
    hsa-miR-3163 hsa-miR-216b hsa-miR-216a
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    GenScript: all cDNA clones in your preferred vector: RLTPR (NM_001013838)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RLTPR
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RLTPR
    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat RLTPR
      QuantiTect SYBR Green Assays in human, mouse, rat RLTPR
      QuantiFast Probe-based Assays in human, mouse, rat RLTPR

    Additional mRNA sequence: 

    AB113647.1 AK090421.1 AL832446.1 BC043154.1 FJ026014.1 

    7 DOTS entries:

    DT.427457  DT.100016955  DT.120660523  DT.100752095  DT.91654589  DT.120660524  DT.75126054 

    Selected AceView cDNA sequences (see all 99):

    AL832446 AI400814 AI438951 BI835866 AW135101 AI693687 AI373410 AI674716 
    AK090421 BX280550 BE207057 BQ286600 BQ957339 BM666528 AI702950 BE999957 
    BE464913 BM695646 AI240176 BE675737 CA865655 BF001222 AI383518 AW166532 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for RLTPR (see all 13)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19a ·
    SP1:                                -     -     -     -     -                             -                                                                     
    SP2:                                                              -                       -                                                                     
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 19b ^ 20 ^ 21 ^ 22a · 22b ^ 23 ^ 24a · 24b ^ 25 ^ 26 ^ 27 ^ 28 ^ 29 ^ 30a · 30b ^ 31a · 31b · 31c ^ 32 ^ 33
    SP1:                                                                                                                        
    SP2:                                                                                                                        
    SP3:                                                                                                                        
    SP4:                                                                                                                        
    SP5:                                                                                -                                       


    ECgene alternative splicing isoforms for RLTPR

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    RLTPR expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AGATTATTTT
    RLTPR Expression
    About this image

    RLTPR Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RLTPR Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.611432

    UniProtKB/Swiss-Prot: LR16C_HUMAN, Q6F5E8
    Tissue specificity: Expressed in all tissues tested, including thymus, spleen, colon, leukocytes, peripheral
    blood, skin, skin keratinocytes and skin fibroblasts

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for RLTPR gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rltpr1 RGD motif, leucine rich repeats, tropomodulin domain more 85.46(n)
    86.45(a)
      234695  NM_001033320.2  NP_001028492.1 
    chicken
    (Gallus gallus)
    Aves RLTPR1 RGD motif, leucine rich repeats, tropomodulin domain more 60.41(n)
    51.63(a)
      415666  XM_414033.4  XP_414033.4 
    lizard
    (Anolis carolinensis)
    Reptilia RLTPR6
    RGD motif, leucine rich repeats, tropomodulin doma...
    42(a)
    1 ↔ 1
    GL343295.1(871622-947995)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia LOC1004917221 leucine-rich repeat-containing protein 16A-like 51.45(n)
    45.81(a)
      100491722  XM_002935368.2  XP_002935414.2 
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkey-6e12.41 si:dkey-6e12.4 53.74(n)
    44.2(a)
      567758  XM_691060.5  XP_696152.5 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG13996
    --
    20(a)
    1 → many
    2R(3564045-3586407)
    worm
    (Caenorhabditis elegans)
    Secernentea crml-16
    Protein CRML-1 (crml-1) mRNA, complete cds
    20(a)
    1 → many
    I(7152460-7156786) WBGene00010641


    ENSEMBL Gene Tree for RLTPR (if available)
    TreeFam Gene Tree for RLTPR (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for RLTPR gene
    LRRC16B2  LRRC16A2  
    3 SIMAP similar genes for RLTPR using alignment to 4 protein entries:     LR16C_HUMAN (see all proteins):
    LRRC16B    FLJ00330    LRRC16A

    Find genes that share paralogs with RLTPR           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for RLTPR
    PGOHUM00000237767


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RLTPR (see all 411)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1887648481,2
    C--67677042(+) AGGCAC/TGGTGT 1 -- us2k10--------
    rs1450440961,2
    --67677144(+) ACCCCA/GTCTCT 1 -- us2k10--------
    rs1475547951,2
    --67677676(+) GACCAC/TAGAGC 1 -- us2k10--------
    rs1175884031,2
    C,F--67677877(+) TGTAAT/CAGAAG 1 -- us2k11Minor allele frequency- C:0.02NA 120
    rs1418615641,2
    --67678103(+) ACTCCA/GGCCCT 1 -- us2k10--------
    rs1471037121,2
    C--67678157(+) TCCCCC/TCGAAG 1 -- us2k10--------
    rs1511142961,2
    --67678162(+) CCGAAA/GGCTCC 1 -- us2k10--------
    rs1913092901,2
    C--67678219(+) AGGGCA/GTCTGG 1 -- us2k10--------
    rs1153528601,2
    F--67678387(+) ACACGC/TCATCA 1 -- us2k11Minor allele frequency- T:0.03WA 118
    rs1160490291,2
    C,F--67678396(+) CACGCC/TGTCTA 1 -- us2k11Minor allele frequency- T:0.06WA 118

    HapMap Linkage Disequilibrium report for RLTPR (67678822 - 67691472 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for RLTPR:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv906809CNV Loss21882294
    dgv2890n71CNV Loss21882294
    dgv2891n71CNV Loss21882294
    nsv524363CNV Loss19592680
    nsv522852CNV Gain19592680

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing RLTPR
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 610859    OMIM disorders: --


    Find genes that share disorders with RLTPR           About GenesLikeMe


    Export disorders for RLTPR gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RLTPR gene integrated from 10 sources:
    (articles sorted by number of sources associating them with RLTPR)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification, expression analysis and polymorphism of a novel RLTPR gene encoding a RGD motif, tropomodulin domain and proline/leucine- rich regions. (PubMed id 15588584)1, 2, 3, 9 Matsuzaka Y.... Inoko H. (Gene 2004)
    2. Distinct roles for CARMIL isoforms in cell migration. (PubMed id 19846667)1, 2, 3 Liang Y.... Cooper J.A. (Mol. Biol. Cell 2009)
    3. Charting the landscape of tandem BRCT domain-mediated protein interactions. (PubMed id 22990118)1 Woods N.T....Monteiro A.N. (Sci Signal 2012)
    4. NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function. (PubMed id 23022380)1 Yatim A....Benkirane M. (Mol. Cell 2012)
    5. N-terminal acetylome analyses and functional insights of the N- terminal acetyltransferase NatB. (PubMed id 22814378)2 Van Damme P....Aldabe R. (Proc. Natl. Acad. Sci. U.S.A. 2012)
    6. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (BMC Syst. Biol. 2011)
    7. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (Sci. Signal. 2009)
    8. A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)2 Dephoure N.... Gygi S.P. (Proc. Natl. Acad. Sci. U.S.A. 2008)
    9. The sequence and analysis of duplication-rich human chromosome 16. (PubMed id 15616553)2 Martin J.... Pennacchio L.A. (Nature 2004)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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      Query String
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 146206 HGNC: 27089 AceView: LOC146206 Ensembl:ENSG00000159753 euGenes: HUgn146206
    ECgene: RLTPR H-InvDB: RLTPR

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for RLTPR Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RLTPR gene:
    Search GeneIP for patents involving RLTPR

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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