RIOK1 Gene
protein-coding GIFtS: 52
GCID: GC06P007335
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|
RIO kinase 1(Previous name: RIO kinase 1 (yeast) )
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Aliases
for RIOK1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| RIO Kinase 11 2 3 | | FLJ300061 | | AD0341 2 | | RIO Kinase 1 (Yeast)1 | | RRP101 2 | | Serine/Threonine-Protein Kinase RIO12 | | BA288G3.11 | | EC 2.7.11.13 |
Export aliases for RIOK1 gene to outside databasesPrevious GC identifers: GC06P007380 GC06P007267 |
Summaries
for RIOK1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for RIOK1:
This gene includes two alternatively spliced transcript variants, which encode different isoforms. The function of thisgene has not been determined. (provided by RefSeq, Jul 2008)
|
Genomic Views
for RIOK1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000006.11 NC_018917.1 NT_007592.15
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the RIOK1 gene promoter:
AREB6 AML1a Pbx1a HTF POU3F2 (N-Oct-5a) POU3F2 (N-Oct-5b) POU3F2 Meis-1b
Other transcription factors
Search SABiosciences Chromatin IP Primers for RIOK1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RIOK1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 6p24.3 Ensembl cytogenetic band: 6p24.3 HGNC cytogenetic band: 6p24.3RIOK1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 6 GeneLoc Exon Structure GeneLoc location for GC06P007335: view genomic region
(about GC identifiers)
Start:
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7,389,729 bp from pter |
End:
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7,418,270 bp from pter |
Size:
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28,542 bases |
Orientation:
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plus strand |
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Proteins
for RIOK1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RIOK1_HUMAN, Q9BRS2 (See
protein sequence)Recommended Name: Serine/threonine-protein kinase RIO1 Size: 568 amino acids; 65583 Da
Secondary accessions: B2RB28 Q8NDC8 Q96NV9Explore the universe of human proteins at neXtProt for RIOK1: NX_Q9BRS2
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9BRS2
RIOK1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_113668.2 NP_694550.1
ENSEMBL proteins: ENSP00000369162 ENSP00000418263
Human Recombinant Protein Products:
RIOK1 Antibody Products:
Assay Products for RIOK1: |
Protein
Domains /
Families
for RIOK1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RIOK1 for domains About GeneDecksing
5 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9BRS2ProtoNet protein and cluster: Q9BRS2 1 Blocks protein family: IPB000687 Protein of unknown function RIO1
UniProtKB/Swiss-Prot: RIOK1_HUMAN, Q9BRS2Similarity: Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase familySimilarity: Contains 1 protein kinase domain |
Function
for RIOK1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RIOK1_HUMAN, Q9BRS2Catalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme Number (IUBMB): EC 2.7.11.11
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RIOK1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RIOK1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): RIOK1 (NM_031480) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RIOK1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RIOK1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RIOK1 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004674 | protein serine/threonine kinase activity |
IEA | -- | | GO:0005524 | ATP binding |
IEA | -- |
RIOK1 for ontologies About GeneDecksing
6 GenomeRNAi human phenotypes for RIOK1:
|
Pathways & Interactions
for RIOK1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Ribosome biogenesis in eukaryotes | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 
Kegg Pathway (Kegg details for RIOK1):
RIOK1 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RIOK1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 17)
5/592 Interacting proteins for RIOK1 (Q9BRS22, 3 ENSP000003691624) via UniProtKB, MINT, STRING, and/or I2D (see all 592)About this table
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Drugs & Compounds
for RIOK1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for RIOK1
2 HMDB Compounds for RIOK1 About this table
Search CenterWatch for drugs/clinical trials and news about RIOK1
|
Transcripts
for RIOK1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for RIOK1 gene (2 alternative transcripts): NM_031480.2 NM_153005.1 Unigene Cluster for RIOK1: RIO kinase 1 Hs.437474 [show with all ESTs]Unigene Representative Sequence: BC0061045 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000379834(uc003mxm.1 uc003mxn.3) ENST00000475351 ENST00000264874(uc003mxo.3)
ENST00000484626 ENST00000493691
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RIOK1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RIOK1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): RIOK1 (NM_031480) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RIOK1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RIOK1 |
Additional cDNA sequence: AF258661.1 AK054568.1 AK314467.1 AL834277.1 AY094356.1 BC000811.2 BC006104.1 8 DOTS entries: DT.453070 DT.97792636 DT.100790350 DT.100790345 DT.100677071 DT.75176060 DT.100790351 DT.91750643 24/140 AceView cDNA sequences (see all 140): BE218823 CA437568 CR608910 CB048066 BF475632 NM_031480 BI858190 AK054568 AI688686 BM987716 BC006104 D11986 BG034097 BG644304 AI990395 AI962832 AF258661 AI651413 AL834277 AV708511 AI688697 BQ448589 BM669627 BC000811
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for RIOK1 About this scheme
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | ^ | 4a | · | 4b | ^ | 5 | ^ | 6 | ^ | 7a | · | 7b | ^ | 8 | ^ | 9a | · | 9b | ^ | 10 | ^ | 11a | · | 11b | ^ | 12 | ^ | 13 | ^ | 14 | ^ | 15 | ^ | 16 | ^ | 17 |
|---|
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| SP1: | | | | | - | | | | | | | | | | | | | | | | | | - | | | | | | | | - | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | - | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for RIOK1
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Expression
for RIOK1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RIOK1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CCCGCCCCAA
About this image
See RIOK1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RIOK1
SOURCE GeneReport for Unigene cluster: Hs.437474
SABiosciences Custom PCR Arrays for RIOK1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RIOK1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RIOK1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RIOK1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RIOK1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RIOK1 |
Orthologs
for RIOK1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for RIOK1 gene from 10/32 species (see all 32) About this table
ENSEMBL Gene Tree for RIOK1 (if available)
TreeFam Gene Tree for RIOK1 (if available) |
Paralogs
for RIOK1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for RIOK1 gene
- RIOK32
1 SIMAP similar gene for RIOK1 using alignment to 2 protein entries: RIOK1_HUMAN (see all proteins):RIOK3
RIOK1 for paralogs About GeneDecksing
|
Genomic Variants
for RIOK1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 6 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for RIOK1 (7389729 - 7418270 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for RIOK1: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RIOK1 |
|
Disorders
/ Diseases
for RIOK1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RIOK1 for disorders About GeneDecksing
2  diseases for RIOK1: About MalaCardscolon cancer malaria
Export disorders for RIOK1 gene to outside databases
|
Publications
for RIOK1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RIOK1 gene, integrated from 9 sources (see all 19):
(articles sorted by number of sources associating them with RIOK1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- The kinase activity of human Rio1 is required for fina l steps of cytoplasmic maturation of 40S subunits. (PubMed id 22072790)1 Widmann B....Kutay U. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
- Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- RioK1, a new interactor of protein arginine methyltra nsferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. (PubMed id 21081503)1 Guderian G....Grimmler M. (2011)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
|
External Searches for RIOK1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing RIOK1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RIOK1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RIOK1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for RIOK1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for RIOK1 gene:
Search GeneIP for patents involving RIOK1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for RIOK1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for RIOK1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RIOK1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RIOK1 | | | OriGene Protein Over-expression Lysate for RIOK1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for RIOK1 | | OriGene 3'-UTR Clone for RIOK1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RIOK1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RIOK1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for RIOK1 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for RIOK1 | | OriGene Custom Protein Services for RIOK1 | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RIOK1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RIOK1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RIOK1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RIOK1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RIOK1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RIOK1 |
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| |  |  |  |  | | | | |
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 | | | RIOK1 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RIOK1 |
|  |  |  | | | | ThermoFisher Antibodies for RIOK1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RIOK1 |
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