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RHOG Gene

protein-coding   GIFtS: 54

GC11M003804
ras homolog gene family, member G (rho G)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: ARHG)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
ARHG 2, 3
MGC125835 1, 2
MGC125836 1, 2
OTTHUMP00000014180 2
OTTHUMP00000014181 2
RhoG 1
Descriptions
ras homolog gene family, member G 2
ras homolog gene family, member G (rho G) 2
External Ids
HGNC: 6721
Entrez Gene: 3912
UniProtKB: P840953
Ensembl: ENSG000001771057
Search outside databases for aliases for RHOG gene

Previous GC identifer: GC11M003813

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for RHOG:
ARHG is a member of the RAS superfamily of genes, which encode GTP-binding proteins that act in the
pathway of signal transduction and play a key role in the regulation of cellular
functions.[supplied by OMIM]

UniProtKB/Swiss-Prot: RHOG_HUMAN, P84095
Function: Required for the formation of membrane ruffles during macropinocytosis. Required for the
formation of cup-like structures during trans-endothelial migration of leukocytes. In case of
Salmonella enterica infection, activated by SopB and SGEF, which induces cytoskeleton
rearrangements and promotes bacterial entry

Gene Wiki entry for RHOG (RhoG)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RHOG gene  

Entrez Gene cytogenetic band: 11p15.5-p15.4   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15.5-p15.4

RHOG Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M003804:     (about GC identifiers)

Start:
3,804,784 bp from pter
End:
3,818,789 bp from pter
Size:
14,006 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_009237.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: RHOG_HUMAN, P84095 (See protein sequence)
Recommended Name: Rho-related GTP-binding protein RhoG precursor  
Size: 191 amino acids; 21309 Da
Subunit: Interacts with SGEF
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential)
Secondary accessions: P35238 Q8NI04

REFSEQ proteins: NP_001656.2  

ENSEMBL proteins: 
ENSP00000380175 ENSP00000380176 ENSP00000339467 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (RHOG)
Human Recombinant Proteins from Abnova (RHOG)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005622 intracellular IEA--
GO:0005886 plasma membrane IEA--
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Antibodies for RHOG: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (RHOG), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (RHOG)
Novus Biologicals Antibodies for RHOG

Assays for RHOG: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

4 InterPro domains/families:
 IPR013753 Ras
 IPR003578 GTPase_Rho
 IPR001806 Ras_GTPase
 IPR005225 Small_GTP_bd


   GeneDecks  RHOG for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P84095

ProtoNet protein and cluster: P84095

UniProtKB/Swiss-Prot: RHOG_HUMAN, P84095
Similarity: Belongs to the small GTPase superfamily. Rho family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RHOG)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RHOG)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001665

              Applied Biosystems Silencer® siRNAs for RHOG

              Sigma-Aldrich siRNA and siRNA Panels for RHOG  
                     Sigma-Aldrich shRNA for RHOG  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for RHOG
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001665
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001665
                                 untagged cDNA clone in CMV expression vector: NM_001665 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001665

UniProtKB/Swiss-Prot: RHOG_HUMAN, P84095
Function: Required for the formation of membrane ruffles during macropinocytosis. Required for the
formation of cup-like structures during trans-endothelial migration of leukocytes. In case of
Salmonella enterica infection, activated by SopB and SGEF, which induces cytoskeleton
rearrangements and promotes bacterial entry

3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Rhog):

hematopoietic systemimmune systemnervous system

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003924 GTPase activity TAS1620121
GO:0005515 protein binding IPI12879077
GO:0005525 GTP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

2 Invitrogen iPath™ Online BioAtlas - Pathways for RHOG (Maps provided by GeneGo):
 Cytoplasm/mitochondrial transport of proapoptotic proteins Bad and Bax
 TNFR1 signaling pathway

   GeneDecks  RHOG for the pathways selected above  
About GeneDecksing

5/11 Sigma-Aldrich "Your Favorite Gene" Pathways for  RHOG  (Your Favorite Gene powered by Ingenuity) (see all 11
 Regulation of Actin-based Motility by Rho
 Germ Cell-Sertoli Cell Junction Signaling
 HMGB1 Signaling
 Integrin Signaling
 Cholecystokinin/Gastrin-mediated Signaling

   GeneDecks  RHOG for the pathways selected above  
About GeneDecksing

 Gene Network CentralTM Interacting Genes and Proteins Network for  RHOG 


5/136 Interacting proteins for RHOG (ENSP000003394673 P840951) via UniProtKB, MINT, and/or STRING (see all 136 )
InteractantInteraction Details
GeneCardExternal ID(s)
ELMO1Q925561STRING (score=.937) EBI-446579, EBI-346417
MCF2ENSP000003596103STRING (score=.998)
TRIOENSP000003392993STRING (score=.993)
VAV2ENSP000003172583STRING (score=.993)
KALRNENSP000003531093STRING (score=.992)
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007264 small GTPase mediated signal transduction IEA--
GO:0007266 Rho protein signal transduction TAS1620121
GO:0008284 positive regulation of cell proliferation TAS1620121
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for RHOG
Browse Small Molecules at Sigma-Aldrich

Compounds for RHOG available from Tocris Bioscience
CompoundAction CAS number
Anti-RacGAPAntibody recognizing RacGAP
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2 Novoseek chemical compound relationships for RHOG gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
gdp 67.45 1 8939998 (1)
gtp 56.88 2 8939998 (2)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RHOG)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RHOG)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001665

              Sigma-Aldrich siRNA and siRNA Panels for RHOG  
                     Sigma-Aldrich shRNA for RHOG  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001665  

REFSEQ mRNAs for RHOG gene: 

NM_001665.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001665  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001665
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001665
                                 untagged cDNA clone in CMV expression vector: NM_001665 

Additional cDNA sequence: 

AF498974.1 AY563952.1 BC104177.2 BC104178.2 BT019872.1 CR450335.1 CR542105.1 CR595510.1 
CR610572.1 CR611274.1 CR611277.1 CR612317.1 CR614056.1 CR616579.1 CR625234.1 CR625567.1 
L11317.1 X61587.1 

7 DOTS entries:

DT.95308177  DT.100761948  DT.316427  DT.100652041  DT.92429039  DT.95149185  DT.86851986 

24/289 AceView cDNA sequences (see all 289 ):

BQ673820 BU617808 AI288206 CK902612 BM543859 BM912761 BU632944 CD674008 
AU104863 CR595510 BI753210 BM553386 BM851558 BF128629 CR611274 CR610572 
BP353272 R76313 AA613507 BI754469 AA010646 BX448423 CR611277 BQ688619 

highest scoring ESTs for RHOG:

X61587 AU099257 AW193418 BE241849 BE408253 BE409267 BE728903 BE736236 BE872106 BF237664 

Unigene Cluster for RHOG:

Ras homolog gene family, member G (rho G)
Hs.501728  [show with all ESTs]
Unigene Representative Sequence: BM912141


GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:
ENST00000396978  ENST00000396979  ENST00000351018  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

RHOG expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RHOG

1 / 2 / 3

3 probe-sets matching RHOG gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

36902_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 X61587 1.00 1.00 1.00 1

203175_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001665 0.60 1.00 0.82 1

203175_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CTTCTGGGGA

SOURCE GeneReport for Unigene cluster: Hs.501728

Expression variation in blood from EXPOLDB for RHOG

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RHOG gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RHOG1   -- ras homolog gene family, member G (rho G) 93.54(n)
99.48(a)
485217  XM_542335.2  XP_542335.2 
cow
(Bos taurus)
RHOG1   -- ras homolog gene family, member G (rho G) 93.02(n)
98.95(a)
538559  XM_581132.3  XP_581132.3 
rat
(Rattus norvegicus)
Rhog1   -- ras homolog gene family, member G (rho G) 91.8(n)
100(a)
308875  NM_001037195.1  NP_001032272.1 
mouse
(Mus musculus)
Rhog1, 5 75
ras homolog gene family, member G1, 5 90.92(n)1
100(a)1
562121  NM_019566.21  NP_062512.11 
 AB0259435  AK1436235  (see all 12)
zebrafish
(Danio rerio)
wufk30f012   -- wufk30f01 77.73(n) 336933  BC044508.1 
About this table        Species with no ortholog for RHOG

ENSEMBL Gene Tree for RHOG
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for RHOG gene
RAC12  RAC22  RAC32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/115 NCBI SNPs in RHOG are shown (see all 115 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 74)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 11 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs10493881,2
A,C,F,H3804949(-) ATGCTC/GTCTCC 1 -- ut31 ese3 trp39Minor allele frequency- G:0.07MN EU EA WA 1016
rs49108521,2
A,F,H3804662(+) AATTGA/GGGGGT 1 -- ng514Minor allele frequency- G:0.08EU EA WA 390
rs79346501,2
C,F,H3820294(+) accacA/Gtaagt 1 -- ng318Minor allele frequency- G:0.16EU EA WA 836
--
rs110300221,2
A,F3819364(+) GCAGCT/CCTGCG 1 -- ng312Minor allele frequency- C:0.47EA MN 400
rs44068201,2
C,F3819109(+) CTGGTG/CCCTTC 1 -- ng312Minor allele frequency- C:0.21EA MN 400
--
rs108351851,2
A,C3818919(+) GCCACG/TTTCCC 1 -- ng310--------
--
rs79315961,2
A,C3820064(+) atgcaA/Gttatc 1 -- ng310--------
rs171738791,2
F,H3804870(+) TGCGTA/GAGGCC 1 -- ut317Minor allele frequency- G:0.01NA EU EA WA 562
rs122899891,2
H3805068(+) ATGAAC/ATGGTC 1 -- ut31 ese34Minor allele frequency- A:0.00EU EA WA 420
rs122762711,2
H3804751(+) CACCCG/ACTAAT 1 -- ng514Minor allele frequency- A:0.00EU EA WA 416
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HapMap Linkage Disequilibrium images for RHOG (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 179505

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/40 PubMed articles for RHOG gene (see all 40 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 391 HGNC: 672 AceView: RHOG Ensembl:ENSG00000177105 euGenes: HUgn391
ECgene: RHOG H-InvDB: RHOG
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for RHOG Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RHOG:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for RHOG-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for RHOG Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for RHOG Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
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 Recombinant & Natural Proteins  

 Recombinant Proteins (RHOG)
 Antibodies (RHOG)
 Chimera RNAi (RHOG)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(RHOG)
 Antibodies (RHOG)
 Search OriGene for RHOG
 Tocris compounds for RHOG




 Drugs for RHOG
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for RHOG

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