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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RHOF Gene

protein-coding   GIFtS: 51
GCID: GC12M122215

ras homolog family member F (in filopodia)

(Previous names: ras homolog gene family, member F (in filopodia) )
(Previous symbol: ARHF)
 Explore 3 diseases affiliated with
RHOF via our new
 Human Malady Compendium 
Biological research products
for RHOF
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Ras Homolog Family Member F (In Filopodia)1 2     Rho Family GTPase Rif2 3
ARHF1 2 3     Rho In Filopodia2 3
RIF1 2 3     FLJ202471
Ras Homolog Gene Family, Member F (In Filopodia)1 2     Rho-Related GTP-Binding Protein RhoF2

External Ids:    HGNC: 157031   Entrez Gene: 545092   Ensembl: ENSG000001397257   UniProtKB: Q9HBH03   

Export aliases for RHOF gene to outside databases

Previous GC identifers: GC12M120615 GC12M120678 GC12M120700 GC12M119227


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RHOF_HUMAN, Q9HBH0
Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound
state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with
CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009775.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RHOF gene promoter:
         AREB6   USF1   p53   USF-1   HOXA5   Olf-1   MyoD   Lmo2   Ik-3   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRHOF promoter sequence
   Search SABiosciences Chromatin IP Primers for RHOF

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RHOF


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q24.31   Ensembl cytogenetic band:  12q24.31   HGNC chromosome: 12

RHOF Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RHOF gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M122215:  view genomic region     (about GC identifiers)

Start:
122,215,660 bp from pter      End:
122,232,261 bp from pter
Size:
16,602 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RHOF_HUMAN, Q9HBH0 (See protein sequence)
Recommended Name: Rho-related GTP-binding protein RhoF precursor  
Size: 211 amino acids; 23625 Da
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cytoplasm, cytoskeleton
Secondary accessions: Q8WVB1 Q9NXH6
Alternative splicing: 2 isoforms:  Q9HBH0-1   Q9HBH0-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for RHOF: NX_Q9HBH0

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9HBH0

  • RHOF Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_061907.2  
    ENSEMBL proteins: 
     ENSP00000267205   ENSP00000440397  
    Reactome Protein details: Q9HBH0
    Human Recombinant Protein Products: 
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    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for RHOF

    Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005829cytosol TAS--
    GO:0005856cytoskeleton NAS11084341
    GO:0005886plasma membrane TAS--
    GO:0016020membrane ----


    RHOF for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for RHOF


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RHOF for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR003578 Small_GTPase_Rho
     IPR005225 Small_GTP-bd_dom
     IPR001806 Small_GTPase

    Graphical View of Domain Structure for InterPro Entry Q9HBH0

    ProtoNet protein and cluster: Q9HBH0

    2 Blocks protein families:
    IPB001806 Transforming protein P21 RAS signature
    IPB003578 Ras small GTPase


    UniProtKB/Swiss-Prot: RHOF_HUMAN, Q9HBH0
    Similarity: Belongs to the small GTPase superfamily. Rho family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RHOF_HUMAN, Q9HBH0
    Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound
    state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with
    CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology

    miRNA
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    hsa-miR-498 hsa-miR-637 hsa-miR-625 hsa-miR-1252 hsa-miR-1291
    SwitchGear 3'UTR luciferase reporter plasmidRHOF 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RHOF (see all 4)
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003924GTPase activity NAS11084341
    GO:0005525GTP binding IEA--


    RHOF for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for RHOF:
     Decreased TP53 protein express 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Cell death signalling via NRAGE, NRIF and NADE
    Signaling by Rho GTPases0.25
    Rho GTPase cycle0.25
    2Signaling by GPCR
    Signal Transduction0.56

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3        Reactome Pathways for RHOF
        Signaling by Rho GTPases
    Signal Transduction
    Rho GTPase cycle



    RHOF for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RHOF

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/68 Interacting proteins for RHOF (Q9HBH03 ENSP000002672054) via UniProtKB, MINT, STRING, and/or I2D (see all 68)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DIAPH3Q9NSV43I2D: score=1 
    ARHGAP15ENSP000002950954STRING: ENSP00000295095
    ARHGAP35ENSP000003857204STRING: ENSP00000385720
    ARHGDIAENSP000002693214STRING: ENSP00000269321
    ARHGDIBENSP000002289454STRING: ENSP00000228945
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006184GTP catabolic process NAS11084341
    GO:0007015actin filament organization IDA11084341
    GO:0007264small GTPase mediated signal transduction TAS--
    GO:0051056regulation of small GTPase mediated signal transduction TAS--


    RHOF for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RHOF

    1 HMDB Compound for RHOF    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--
    Search CenterWatch for drugs/clinical trials and news about RHOF 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RHOF gene: 
    NM_019034.2  

    Unigene Clusters for RHOF:

    Ras homolog family member F (in filopodia)
    Hs.644504  [show with all ESTs], Hs.717201  [show with all ESTs]
    Unigene Representative Sequences: NM_001080825, NM_019034
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000267205(uc001ubb.3) ENST00000537265 ENST00000537171(uc001ubd.4)
    ENST00000535560

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    hsa-miR-498 hsa-miR-637 hsa-miR-625 hsa-miR-1252 hsa-miR-1291
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    Inhib. RNA
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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RHOF

    Additional cDNA sequence: 

    AF239923.1 AK000254.1 AK289482.1 BC018208.1 

    17 DOTS entries:

    DT.209602  DT.411865  DT.100810676  DT.40128011  DT.101981211  DT.95358516  DT.121209862  DT.121209861 
    DT.100641705  DT.97818067  DT.100720727  DT.100649089  DT.121209817  DT.91746743  DT.92059095  DT.102832696 
    DT.91905495 

    1 AceView cDNA sequence:

    AK130459 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RHOF expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AGCGGCAGCC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    RHOF expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    LiverLiver BudLiver
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Definitive endoderm-like cells (A scalable, suspensi...)

    See RHOF Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RHOF

    SOURCE GeneReport for Unigene clusters: Hs.644504 Hs.717201
        SABiosciences Custom PCR Arrays for RHOF
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RHOF

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for RHOF gene from 4/19 species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves RHOF1 ras homolog family member F (in filopodia) 74.84(n)
    77.42(a)
      416848  NM_001030660.1  NP_001025831.1 
    lizard
    (Anolis carolinensis)
    Reptilia RHOF6
    --
    67(a)
    1 ↔ 1
    GL343550.1(228074-237566)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.165832 Xenopus laevis transcribed sequence with moderate similarity more 75.11(n)    BU914775.1 
    zebrafish
    (Danio rerio)
    Actinopterygii rhof1 ras homolog gene family, member F 62.69(n)
    65.67(a)
      573655  NM_001020642.1  NP_001018478.1 


    ENSEMBL Gene Tree for RHOF (if available)
    TreeFam Gene Tree for RHOF (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RHOF gene
    RHOB2  RHOD2  RND12  RHOA2  RHOC2  RND32  
    18/51 SIMAP similar genes for RHOF using alignment to 2 protein entries:     RHOF_HUMAN (see all proteins) (see all similar genes):
    RHOC    RHOJ    RHOA    RHOB    RAC3    RAC2
    CDC42    RHOQ    ARHG    RHOG    RHOH    ARHE
    RAC1    RND2    RND3    ARHH    RAB28    RHOD

    RHOF for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for RHOF
    PGOHUM00000251009


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/334 NCBI SNPs in RHOF are shown (see all 334    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs666826271,2
    C--119238009(+) AAAAA-/AGGGGGG 1 -- int11Minor allele frequency- AG:0.00NA 2
    rs734137171,2
    C,--122215196(+) AATACT/ATTTTA 2 -- int1 ut317Minor allele frequency- A:0.30WA NA CSA EA 365
    rs734137201,2
    C,--122215259(+) CAACTC/TATCCT 2 -- ut31 int17Minor allele frequency- T:0.29WA NA CSA EA 365
    rs1817778781,2
    --122215356(+) CCCCTA/CCAGTG 2 -- int1 ut310--------
    rs1427143961,2
    --122215405(+) AAAAAC/TGGACC 2 -- int1 ut310--------
    rs1856393621,2
    --122215439(+) ACGCCC/TACTCT 2 -- int1 ut310--------
    rs116111381,2
    C,F,A,H,--122215441(+) GCCTAC/GTCTCC 2 -- int1 ut316Minor allele frequency- G:0.01NS EA NA 542
    rs1497183931,2
    --122215484(+) TCATTC/TTGGTC 2 -- ut31 int10--------
    rs10641691,2
    C,F,O,H,--122215520(+) TGCGTT/CTGCCC 2 -- ut31 int127Minor allele frequency- C:0.31MN NS EA NA WA CSA 2749
    rs37823331,2
    H--122215557(+) TAAAGG/TGCCAG 2 -- int1 ut314Minor allele frequency- T:0.00NS EA 416

    HapMap Linkage Disequilibrium report for RHOF (122215660 - 122232261 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for RHOF
         1 CNV: 76168

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RHOF for disorders           About GeneDecksing

    3 diseases for RHOF:    About MalaCards
    mycosis fungoides    lung cancer    malaria

    1 Novoseek disease relationship for RHOF gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    malaria 62.7 3 17196595 (1), 14573641 (1), 12438381 (1)


    Export disorders for RHOF gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RHOF gene, integrated from 9 sources (see all 21):
    (articles sorted by number of sources associating them with RHOF)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The novel Rho-family GTPase rif regulates coordinated actin-based membrane rearrangements. (PubMed id 11084341)1, 2, 3, 9 Ellis S. and Mellor H. (2000)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. The interplay between the Escherichia coli Rho guanine nucleotide exchange factor effectors and the mammalian RhoGEF inhibitor EspH. (PubMed id 22251971)1 Wong A.R....Frankel G. (2012)
    5. The small Rho GTPase Rif and actin cytoskeletal remode lling. (PubMed id 22260703)1 Fan L. and Mellor H. (2012)
    6. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    7. The small GTPase Rif is an alternative trigger for th e formation of actin stress fibers in epithelial cells. (PubMed id 20233848)1 Fan L....Mellor H. (2010)
    8. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
    9. Array-based comparative genomic hybridization in early-stage mycosis fungoides: recurrent deletion of tumor suppressor genes BCL7A, SMAC/DIABLO, and RHOF. (PubMed id 18663754)1 Carbone A....Amerio P. (2008)
    10. Transcriptome analysis of human gastric cancer. (PubMed id 16341674)1 Oh J.H....Kim N.S. (2005)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54509 HGNC: 15703 AceView: RHOF.1 Ensembl:ENSG00000139725 euGenes: HUgn54509
    ECgene: RHOF H-InvDB: RHOF

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RHOF Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RHOF gene:
    Search GeneIP for patents involving RHOF

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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