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Aliases for RHOA Gene

Aliases for RHOA Gene

  • Ras Homolog Family Member A 2 3
  • ARH12 3 4 6
  • ARHA 3 4 6
  • Ras Homolog Gene Family, Member A 2 3
  • Rho CDNA Clone 12 3 4
  • RHOH12 3 6
  • RHO12 3 4
  • H12 3 4
  • Aplysia Ras-Related Homolog 12 3
  • Small GTP Binding Protein RhoA 3
  • Transforming Protein RhoA 3
  • Oncogene RHO H12 3

External Ids for RHOA Gene

Previous Symbols for RHOA Gene

  • ARH12
  • ARHA

Summaries for RHOA Gene

GeneCards Summary for RHOA Gene

RHOA (Ras Homolog Family Member A) is a Protein Coding gene. Among its related pathways are PI-3K cascade and Signaling by FGFR. GO annotations related to this gene include GTP binding and protein domain specific binding. An important paralog of this gene is RHOB.

UniProtKB/Swiss-Prot for RHOA Gene

  • Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436,PubMed:19403695).

Gene Wiki entry for RHOA Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RHOA Gene

Genomics for RHOA Gene

Genomic Location for RHOA Gene

Start:
49,359,145 bp from pter
End:
49,412,998 bp from pter
Size:
53,854 bases
Orientation:
Minus strand

Genomic View for RHOA Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RHOA Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RHOA Gene

Regulatory Elements for RHOA Gene

Proteins for RHOA Gene

  • Protein details for RHOA Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61586-RHOA_HUMAN
    Recommended name:
    Transforming protein RhoA
    Protein Accession:
    P61586
    Secondary Accessions:
    • P06749
    • Q53HM4
    • Q5U024
    • Q9UDJ0
    • Q9UEJ4

    Protein attributes for RHOA Gene

    Size:
    193 amino acids
    Molecular mass:
    21768 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with ARHGEF28 (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2. Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB. Interacts (GTP-bound form) with KCNA2 (via cytoplasmic N-terminal domain) (PubMed:9635436).

    Three dimensional structures from OCA and Proteopedia for RHOA Gene

neXtProt entry for RHOA Gene

Proteomics data for RHOA Gene at MOPED

Post-translational modifications for RHOA Gene

  • AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
  • Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
  • Substrate for botulinum ADP-ribosyltransferase
  • Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys104 and Lys135

Other Protein References for RHOA Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for RHOA Gene

Domains for RHOA Gene

Protein Domains for RHOA Gene

Suggested Antigen Peptide Sequences for RHOA Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P61586

UniProtKB/Swiss-Prot:

RHOA_HUMAN
Domain:
  • The basic-rich region is essential for yopT recognition and cleavage:
    • P61586
Family:
  • Belongs to the small GTPase superfamily. Rho family.:
    • P61586
genes like me logo Genes that share domains with RHOA: view

No data available for Gene Families for RHOA Gene

Function for RHOA Gene

Molecular function for RHOA Gene

UniProtKB/Swiss-Prot EnzymeRegulation: GTP hydrolysis is stimulated by ARHGAP30.
UniProtKB/Swiss-Prot Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436,PubMed:19403695).

Gene Ontology (GO) - Molecular Function for RHOA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003924 GTPase activity TAS 10436159
GO:0005515 protein binding IPI 10051605
GO:0005525 GTP binding IEA --
GO:0017022 myosin binding IPI 15644318
genes like me logo Genes that share ontologies with RHOA: view
genes like me logo Genes that share phenotypes with RHOA: view

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for RHOA Gene

Localization for RHOA Gene

Subcellular locations from UniProtKB/Swiss-Prot for RHOA Gene

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Cell projection, lamellipodium. Note=Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RHOA Gene COMPARTMENTS Subcellular localization image for RHOA gene
Compartment Confidence
cytoskeleton 5
plasma membrane 5
cytosol 4
mitochondrion 2
nucleus 2
extracellular 1

Gene Ontology (GO) - Cellular Components for RHOA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular --
GO:0005789 endoplasmic reticulum membrane TAS --
GO:0005829 cytosol TAS --
GO:0005856 cytoskeleton IEA --
GO:0005886 plasma membrane TAS --
genes like me logo Genes that share ontologies with RHOA: view

Pathways for RHOA Gene

genes like me logo Genes that share pathways with RHOA: view

Gene Ontology (GO) - Biological Process for RHOA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006184 obsolete GTP catabolic process --
GO:0007165 signal transduction --
GO:0007179 transforming growth factor beta receptor signaling pathway TAS --
GO:0007264 small GTPase mediated signal transduction TAS --
GO:0007266 Rho protein signal transduction TAS 10436159
genes like me logo Genes that share ontologies with RHOA: view

Compounds for RHOA Gene

(4) HMDB Compounds for RHOA Gene

Compound Synonyms Cas Number PubMed IDs
Atorvastatin
  • Atorvastatin acid
134523-00-5
Guanosine triphosphate
  • 5'-GTP
86-01-1
Magnesium
  • Magnesium
7439-95-4
Simvastatin
  • (+)-Simvastatin
79902-63-9

(1) Drugbank Compounds for RHOA Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
Guanosine-5'-Diphosphate
146-91-8 target

(107) Novoseek inferred chemical compound relationships for RHOA Gene

Compound -log(P) Hits PubMed IDs
geranylgeranyl pyrophosphate 85.4 19
mdi-a 83.6 16
gdp 71.8 30
fasudil 71 5
actomyosin 71 19
genes like me logo Genes that share compounds with RHOA: view

Transcripts for RHOA Gene

Unigene Clusters for RHOA Gene

Ras homolog family member A:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RHOA Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c
SP1:
SP2: -
SP3: - - -
SP4: - - -

Relevant External Links for RHOA Gene

GeneLoc Exon Structure for
RHOA
ECgene alternative splicing isoforms for
RHOA

Expression for RHOA Gene

mRNA expression in normal human tissues for RHOA Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RHOA Gene

This gene is overexpressed in Whole Blood (4.2).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RHOA Gene

SOURCE GeneReport for Unigene cluster for RHOA Gene Hs.247077

genes like me logo Genes that share expressions with RHOA: view

No data available for mRNA Expression by UniProt/SwissProt for RHOA Gene

Orthologs for RHOA Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RHOA Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RHOA 35
  • 100 (n)
  • 100 (a)
RHOA 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RHOA 35
  • 93.96 (n)
  • 100 (a)
RHOA 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RHOA 35
  • 95.34 (n)
  • 100 (a)
RHOA 36
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia 4930544G11Rik 36
  • 84 (a)
OneToOne
Rhoa 35
  • 93.78 (n)
  • 99.48 (a)
Rhoa 16
oppossum
(Monodelphis domestica)
Mammalia RHOA 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RHOA 36
  • 96 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rhoa 35
  • 94.13 (n)
  • 99.48 (a)
chicken
(Gallus gallus)
Aves CRHOA 36
  • 99 (a)
OneToMany
RHOA 35
  • 83.77 (n)
  • 99.48 (a)
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 99 (a)
ManyToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia rhoa.2 35
  • 77.2 (n)
  • 92.23 (a)
Str.942 35
zebrafish
(Danio rerio)
Actinopterygii rhoaa 36
  • 93 (a)
ManyToMany
rhoab 35
  • 74.96 (n)
  • 95.85 (a)
wufc17g01 35
fruit fly
(Drosophila melanogaster)
Insecta Rho1 36
  • 87 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea rho-1 36
  • 88 (a)
OneToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ABR183W 35
  • 62.37 (n)
  • 67.74 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RHO1 35
  • 63.7 (n)
  • 72.25 (a)
RHO1 36
  • 67 (a)
OneToMany
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B10626g 35
  • 63.39 (n)
  • 68.39 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU01484 35
  • 64.2 (n)
  • 71.96 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rho1 35
  • 63.33 (n)
  • 70 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.5323 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.11308 36
  • 86 (a)
OneToMany
Species with no ortholog for RHOA:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RHOA Gene

ENSEMBL:
Gene Tree for RHOA (if available)
TreeFam:
Gene Tree for RHOA (if available)

Paralogs for RHOA Gene

Paralogs for RHOA Gene

Pseudogenes.org Pseudogenes for RHOA Gene

genes like me logo Genes that share paralogs with RHOA: view

Variants for RHOA Gene

Sequence variations from dbSNP and Humsavar for RHOA Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs892974 -- 49,361,081(-) GTTTT(G/T)TTTTT intron-variant
rs974495 -- 49,363,049(-) AAAGA(A/G)CATGT intron-variant
rs1164157 -- 49,399,277(-) ttctc(C/T)tgcct intron-variant
rs1177410 -- 49,399,351(-) gctct(C/G/T)tcgcc intron-variant
rs1182794 -- 49,399,294(-) ctccc(A/G)ggttc intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RHOA Gene

Variant ID Type Subtype PubMed ID
nsv236940 CNV Loss 16902084
nsv237162 CNV Loss 16902084
nsv834687 CNV Loss 17160897

Relevant External Links for RHOA Gene

HapMap Linkage Disequilibrium report
RHOA

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RHOA Gene

Disorders for RHOA Gene

(68) Novoseek inferred disease relationships for RHOA Gene

Disease -log(P) Hits PubMed IDs
metastasis 41.1 28
cancer 36.9 42
tumors 32.7 89
cardiac hypertrophy 22 2
prostate cancer 21.2 12

Relevant External Links for RHOA

Genetic Association Database (GAD)
RHOA
Human Genome Epidemiology (HuGE) Navigator
RHOA
genes like me logo Genes that share disorders with RHOA: view

No data available for UniProtKB/Swiss-Prot for RHOA Gene

Publications for RHOA Gene

  1. Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling. (PMID: 11696353) Klussmann E. … Rosenthal W. (FEBS Lett. 2001) 3 4 23
  2. XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC. (PMID: 12221096) Arthur W.T. … Wennerberg K. (J. Biol. Chem. 2002) 3 4 23
  3. Consequences of mevalonate depletion. Differential transcriptional, translational, and post-translational up-regulation of Ras, Rap1a, RhoA, AND RhoB. (PMID: 11788600) Holstein S.A. … Hohl R.J. (J. Biol. Chem. 2002) 3 23
  4. Leukotriene D(4) induces stress-fibre formation in intestinal epithelial cells via activation of RhoA and PKCdelta. (PMID: 12154081) Massoumi R. … SjAPlander A. (J. Cell. Sci. 2002) 3 23
  5. RhoA expression is controlled by nitric oxide through cGMP-dependent protein kinase activation. (PMID: 12524425) Sauzeau V. … Pacaud P. (J. Biol. Chem. 2003) 3 23

Products for RHOA Gene

  • Addgene plasmids for RHOA

Sources for RHOA Gene

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