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Aliases for RHOA Gene

Aliases for RHOA Gene

  • Ras Homolog Family Member A 2 3 5
  • ARH12 3 4
  • RHO12 3 4
  • ARHA 3 4
  • Ras Homolog Gene Family, Member A 2
  • Aplysia Ras-Related Homolog 12 3
  • Small GTP Binding Protein RhoA 3
  • Rho CDNA Clone 12 4
  • Oncogene RHO H12 3
  • RHOH12 3
  • H12 4

External Ids for RHOA Gene

Previous HGNC Symbols for RHOA Gene

  • ARH12
  • ARHA

Previous GeneCards Identifiers for RHOA Gene

  • GC03M049357
  • GC03M049371

Summaries for RHOA Gene

Entrez Gene Summary for RHOA Gene

  • This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

GeneCards Summary for RHOA Gene

RHOA (Ras Homolog Family Member A) is a Protein Coding gene. Diseases associated with RHOA include vaccinia and colorectal cancer. Among its related pathways are Platelet activation, signaling and aggregation and Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include GTP binding and myosin binding. An important paralog of this gene is RHOB.

UniProtKB/Swiss-Prot for RHOA Gene

  • Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).

  • (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Gene Wiki entry for RHOA Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RHOA Gene

Genomics for RHOA Gene

Regulatory Elements for RHOA Gene

Promoters for RHOA Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around RHOA on UCSC Golden Path with GeneCards custom track

Genomic Location for RHOA Gene

Chromosome:
3
Start:
49,359,136 bp from pter
End:
49,412,998 bp from pter
Size:
53,863 bases
Orientation:
Minus strand

Genomic View for RHOA Gene

Genes around RHOA on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RHOA Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RHOA Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RHOA Gene

Proteins for RHOA Gene

  • Protein details for RHOA Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61586-RHOA_HUMAN
    Recommended name:
    Transforming protein RhoA
    Protein Accession:
    P61586
    Secondary Accessions:
    • P06749
    • Q53HM4
    • Q5U024
    • Q9UDJ0
    • Q9UEJ4

    Protein attributes for RHOA Gene

    Size:
    193 amino acids
    Molecular mass:
    21768 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with ARHGEF28 (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with DIAPH1 (PubMed:23325789). Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2. Interacts with ARHGDIA; this interaction inactivates and stabilizes RHOA. Interacts with ARHGDIB. Interacts (GTP-bound form) with KCNA2 (via cytoplasmic N-terminal domain) (PubMed:9635436).

    Three dimensional structures from OCA and Proteopedia for RHOA Gene

neXtProt entry for RHOA Gene

Proteomics data for RHOA Gene at MOPED

Post-translational modifications for RHOA Gene

  • (Microbial infection) AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • (Microbial infection) Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
  • (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.
  • (Microbial infection) Substrate for botulinum ADP-ribosyltransferase.
  • Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling (PubMed:11162591). Phosphorylation by SLK at Ser-188 in response to AGTR2 activation (By similarity).
  • Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • Ubiquitination at Lys 135
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for RHOA (RHOA)

No data available for DME Specific Peptides for RHOA Gene

Domains & Families for RHOA Gene

Gene Families for RHOA Gene

Protein Domains for RHOA Gene

Suggested Antigen Peptide Sequences for RHOA Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P61586

UniProtKB/Swiss-Prot:

RHOA_HUMAN :
  • The basic-rich region is essential for yopT recognition and cleavage.
  • Belongs to the small GTPase superfamily. Rho family.
Domain:
  • The basic-rich region is essential for yopT recognition and cleavage.
Family:
  • Belongs to the small GTPase superfamily. Rho family.
genes like me logo Genes that share domains with RHOA: view

Function for RHOA Gene

Molecular function for RHOA Gene

UniProtKB/Swiss-Prot EnzymeRegulation:
GTP hydrolysis is stimulated by ARHGAP30.
UniProtKB/Swiss-Prot Function:
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).
UniProtKB/Swiss-Prot Function:
(Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
genes like me logo Genes that share phenotypes with RHOA: view

Animal Model Products

miRNA for RHOA Gene

miRTarBase miRNAs that target RHOA

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RHOA Gene

Localization for RHOA Gene

Subcellular locations from UniProtKB/Swiss-Prot for RHOA Gene

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Cell projection, lamellipodium. Note=Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RHOA Gene COMPARTMENTS Subcellular localization image for RHOA gene
Compartment Confidence
cytoskeleton 5
cytosol 5
endoplasmic reticulum 5
extracellular 5
plasma membrane 5
endosome 4
nucleus 3
mitochondrion 2
golgi apparatus 1
lysosome 1
vacuole 1

Gene Ontology (GO) - Cellular Components for RHOA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005938 cell cortex IDA 16103226
GO:0071944 cell periphery IMP 25911094
genes like me logo Genes that share ontologies with RHOA: view

Pathways & Interactions for RHOA Gene

genes like me logo Genes that share pathways with RHOA: view

Pathways by source for RHOA Gene

SIGNOR curated interactions for RHOA Gene

Gene Ontology (GO) - Biological Process for RHOA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007179 transforming growth factor beta receptor signaling pathway TAS --
GO:0007264 small GTPase mediated signal transduction IEA --
GO:0007266 Rho protein signal transduction TAS 10436159
GO:0007411 axon guidance TAS --
GO:0007596 blood coagulation TAS --
genes like me logo Genes that share ontologies with RHOA: view

Drugs & Compounds for RHOA Gene

(61) Drugs for RHOA Gene - From: HMDB, Novoseek, PharmGKB, ApexBio, and DrugBank

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Simvastatin Approved Pharma Competitive, Inhibitor HMG-CoA reductase inhibitor 493
Pravastatin Approved Pharma Competitive, Inhibitor 144
atorvastatin Approved Pharma Competitive, Inhibitor 0
Guanosine diphosphate Experimental Pharma Target 0
Guanosine triphosphate Experimental Pharma 0

(50) Additional Compounds for RHOA Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs

(1) ApexBio Compounds for RHOA Gene

Compound Action Cas Number
CCG-1423 RhoA inhibitor 285986-88-1
genes like me logo Genes that share compounds with RHOA: view

Transcripts for RHOA Gene

Unigene Clusters for RHOA Gene

Ras homolog family member A:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RHOA Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c
SP1:
SP2: -
SP3: - - -
SP4: - - -

Relevant External Links for RHOA Gene

GeneLoc Exon Structure for
RHOA
ECgene alternative splicing isoforms for
RHOA

Expression for RHOA Gene

mRNA expression in normal human tissues for RHOA Gene

mRNA differential expression in normal tissues according to GTEx for RHOA Gene

This gene is overexpressed in Whole Blood (x4.2).

Protein differential expression in normal tissues from HIPED for RHOA Gene

This gene is overexpressed in Peripheral blood mononuclear cells (6.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RHOA Gene



SOURCE GeneReport for Unigene cluster for RHOA Gene Hs.247077

genes like me logo Genes that share expression patterns with RHOA: view

Protein tissue co-expression partners for RHOA Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for RHOA Gene

Orthologs for RHOA Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RHOA Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RHOA 35
  • 93.96 (n)
  • 100 (a)
RHOA 36
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RHOA 35
  • 95.34 (n)
  • 100 (a)
RHOA 36
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rhoa 35
  • 93.78 (n)
  • 99.48 (a)
Rhoa 16
4930544G11Rik 36
  • 84 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RHOA 35
  • 100 (n)
  • 100 (a)
RHOA 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rhoa 35
  • 94.13 (n)
  • 99.48 (a)
oppossum
(Monodelphis domestica)
Mammalia RHOA 36
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RHOA 36
  • 96 (a)
OneToOne
chicken
(Gallus gallus)
Aves RHOA 35
  • 83.77 (n)
  • 99.48 (a)
CRHOA 36
  • 99 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 99 (a)
ManyToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia rhoa.2 35
  • 77.2 (n)
  • 92.23 (a)
Str.942 35
zebrafish
(Danio rerio)
Actinopterygii rhoab 35
  • 74.96 (n)
  • 95.85 (a)
wufc17g01 35
rhoaa 36
  • 93 (a)
ManyToMany
fruit fly
(Drosophila melanogaster)
Insecta Rho1 36
  • 87 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea rho-1 36
  • 88 (a)
OneToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ABR183W 35
  • 62.37 (n)
  • 67.74 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B10626g 35
  • 63.39 (n)
  • 68.39 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RHO1 35
  • 63.7 (n)
  • 72.25 (a)
RHO1 36
  • 67 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.5323 35
bread mold
(Neurospora crassa)
Ascomycetes NCU01484 35
  • 64.2 (n)
  • 71.96 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rho1 35
  • 63.33 (n)
  • 70 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.11308 36
  • 86 (a)
OneToMany
Species with no ortholog for RHOA:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RHOA Gene

ENSEMBL:
Gene Tree for RHOA (if available)
TreeFam:
Gene Tree for RHOA (if available)

Paralogs for RHOA Gene

Pseudogenes.org Pseudogenes for RHOA Gene

genes like me logo Genes that share paralogs with RHOA: view

Variants for RHOA Gene

Sequence variations from dbSNP and Humsavar for RHOA Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type
rs892974 -- 49,361,081(-) GTTTT(G/T)TTTTT intron-variant
rs1177410 -- 49,399,351(-) gctct(C/G/T)tcgcc intron-variant
rs974495 -- 49,363,049(-) AAAGA(A/G)CATGT intron-variant
rs1182794 -- 49,399,294(-) ctccc(A/G)ggttc intron-variant
rs1184783 -- 49,399,155(-) cctga(C/G)ctcgt intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RHOA Gene

Variant ID Type Subtype PubMed ID
nsv236940 CNV Loss 16902084
nsv237162 CNV Loss 16902084
nsv834687 CNV Loss 17160897

Variation tolerance for RHOA Gene

Residual Variation Intolerance Score: 46.6% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RHOA Gene

HapMap Linkage Disequilibrium report
RHOA

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RHOA Gene

Disorders for RHOA Gene

MalaCards: The human disease database

(4) MalaCards diseases for RHOA Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
vaccinia
colorectal cancer
  • colorectal cancer, somatic
gastrointestinal defects and immunodeficiency syndrome
  • multiple intestinal atresia
breast cancer
  • breast cancer, invasive ductal
- elite association - COSMIC cancer census association via MalaCards
Search RHOA in MalaCards View complete list of genes associated with diseases

Relevant External Links for RHOA

Genetic Association Database (GAD)
RHOA
Human Genome Epidemiology (HuGE) Navigator
RHOA
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RHOA
genes like me logo Genes that share disorders with RHOA: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RHOA Gene

Publications for RHOA Gene

  1. The armadillo protein p0071 regulates Rho signalling during cytokinesis. (PMID: 17115030) Wolf A. … Hatzfeld M. (Nat. Cell Biol. 2006) 3 4 67
  2. Intact lipid rafts regulate HIV-1 Tat protein-induced activation of the Rho signaling and upregulation of P-glycoprotein in brain endothelial cells. (PMID: 19794400) Zhong Y. … Toborek M. (J. Cereb. Blood Flow Metab. 2010) 3 23
  3. Altered distribution of RhoA in Alzheimer's disease and AbetaPP overexpressing mice. (PMID: 20061625) Huesa G. … Galea E. (J. Alzheimers Dis. 2010) 3 23
  4. Binding of PDZ-RhoGEF to ATP-binding cassette transporter A1 (ABCA1) induces cholesterol efflux through RhoA activation and prevention of transporter degradation. (PMID: 20348106) Okuhira K. … Nishimaki-Mogami T. (J. Biol. Chem. 2010) 3 23
  5. Deciphering the transcriptional complex critical for RhoA gene expression and cancer metastasis. (PMID: 20383141) Chan C.H. … Lin H.K. (Nat. Cell Biol. 2010) 3 23

Products for RHOA Gene

  • Addgene plasmids for RHOA

Sources for RHOA Gene

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