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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RHNO1 Gene

protein-coding   GIFtS: 35
GCID: GC12P002988

RAD9-HUS1-RAD1 interacting nuclear orphan 1

(Previous name: chromosome 12 open reading frame 32 )
(Previous symbol: C12orf32)
 Explore 3 diseases affiliated with
RHNO1 via our new
 Human Malady Compendium 
Biological research products
for RHNO1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3

Aliases
RAD9-HUS1-RAD1 Interacting Nuclear Orphan 11 2     MGC132041
C12orf321 2 3     Chromosome 12 Open Reading Frame 321
RHINO1 2 3     RAD9, HUS1, RAD1-Interacting Nuclear Orphan Protein 12
RAD9, RAD1, HUS1-Interacting Nuclear Orphan Protein2 3     Rad9, Rad1, Hus1 Interacting Nuclear Orphan2
HKMT11881     

External Ids:    HGNC: 282061   Entrez Gene: 836952   Ensembl: ENSG000001717927   OMIM: 6140855   UniProtKB: Q9BSD33   
ORGUL members:         
NONCODE:n407824 n407822    

Export aliases for RHNO1 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RHNO1_HUMAN, Q9BSD3
Function: Plays a role in DNA damage response (DDR) signaling upon genotoxic stresses such as ionizing radiation (IR)
during the S phase. Recruited to sites of DNA damage through interaction with the 9-1-1 cell-cycle checkpoint response
complex and TOPBP1 in a ATR-dependent manner. Required for the progression of the G1 to S phase transition. Plays a
role in the stimulation of CHEK1 phosphorylation




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009759.16  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for RHNO1
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRHNO1 promoter sequence
   Search SABiosciences Chromatin IP Primers for RHNO1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RHNO1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12p13.33   Ensembl cytogenetic band:  12p13.33   HGNC cytogenetic band: 12p13.33

RHNO1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RHNO1 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P002988:  view genomic region     (about GC identifiers)

Start:
2,985,424 bp from pter      End:
2,998,626 bp from pter
Size:
13,203 bases      Orientation:
plus strand
ORGUL member locations:
Legend (see complete legend)

ORGUL Member Locations for RHNO1

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RHNO1_HUMAN, Q9BSD3 (See protein sequence)
Recommended Name: RAD9, HUS1, RAD1-interacting nuclear orphan protein 1  
Size: 238 amino acids; 26709 Da
Subunit: Interacts with RAD9A, RAD18, TOPBP1 and UBE2N
Subcellular location: Nucleus. Chromosome. Note=Localizes to sites of DNA damage in a H2AX-independent manner
Secondary accessions: B7Z989
Alternative splicing: 2 isoforms:  Q9BSD3-1   Q9BSD3-2   

Explore the universe of human proteins at neXtProt for RHNO1: NX_Q9BSD3

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BSD3

  • RHNO1 Protein expression data from MOPED and PaxDb:    About this image 
    RHNO1 Protein Expression
    REFSEQ proteins (4 alternative transcripts): 
    NP_001239428.1  NP_001239429.1  NP_001244026.1  NP_001244027.1  

    ENSEMBL proteins: 
     ENSP00000442319   ENSP00000438828   ENSP00000438590   ENSP00000444654   ENSP00000442677  
     ENSP00000445936  

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    Uscn Proteins for RHNO1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005694chromosome IDA--

    RHNO1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q9BSD3


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RHNO1_HUMAN, Q9BSD3
    Function: Plays a role in DNA damage response (DDR) signaling upon genotoxic stresses such as ionizing radiation (IR)
    during the S phase. Recruited to sites of DNA damage through interaction with the 9-1-1 cell-cycle checkpoint response
    complex and TOPBP1 in a ATR-dependent manner. Required for the progression of the G1 to S phase transition. Plays a
    role in the stimulation of CHEK1 phosphorylation
    Induction: Up-regulated in breast cancer cells

         Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI--
         
    RHNO1 for ontologies           About GeneDecksing


    Animal Models:
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for RHNO1 

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RHNO1


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RHNO1

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000077DNA damage checkpoint IMP--
    GO:0000725recombinational repair IMP--
    GO:0007049cell cycle IEA--
    GO:0034644cellular response to UV IDA--
    GO:0070318positive regulation of G0 to G1 transition IMP--

    RHNO1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RHNO1
    Search CenterWatch for drugs/clinical trials and news about RHNO1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RHNO1 gene (4 alternative transcripts): 
    NM_001252499.2  NM_001252500.2  NM_001257097.1  NM_001257098.1  

    Unigene Cluster for RHNO1:

    RAD9-HUS1-RAD1 interacting nuclear orphan 1
    Hs.198853  [show with all ESTs]
    Unigene Representative Sequence: NR_046432
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000536063 ENST00000538636 ENST00000461997 ENST00000464682 ENST00000489288(uc010see.2 uc001qlh.3 uc001qli.3)
    ENST00000366285 ENST00000535978 ENST00000538700 ENST00000500236

    miRNA
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    Inhib. RNA
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    Additional cDNA sequence: 

    AB073599.1 AK021945.1 AK304613.1 AK315364.1 BC000079.1 BC005106.1 NR_027365.2 NR_046432.1 
    NR_046433.1 

    13 DOTS entries:

    DT.100850973  DT.100812518  DT.100812525  DT.311694  DT.101968499  DT.100812517  DT.100717476  DT.100812522 
    DT.121115265  DT.95119751  DT.100728614  DT.121115279  DT.92406720 

    24/226 AceView cDNA sequences (see all 226):

    BU189103 BM354093 BC005106 N75081 BQ009701 BP365233 BQ001467 AB073599 
    AK021945 BQ932783 CB139524 AA569840 CR606868 CB127339 BQ017907 BM459041 
    CR613904 CR602379 AA306071 NM_031465 CB112608 BE219889 CB126448 BM141952 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for RHNO1    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b
    SP1:                                    
    SP2:        -     -     -               
    SP3:        -                           


    ECgene alternative splicing isoforms for RHNO1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section
    See probesets specificity/sensitivity at GeneAnnot
    CGAP TAG: --

    See RHNO1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RHNO1

    SOURCE GeneReport for Unigene cluster: Hs.198853

    UniProtKB/Swiss-Prot: RHNO1_HUMAN, Q9BSD3
    Tissue specificity: Weakly expressed in testis, prostate, ovary, thymus and small intestine. Expressed strongly in
    breast cancer cells

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RHNO1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for RHNO1 gene from 3/6 species (see all 6)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rhno15 RIKEN cDNA 5930416I19 gene   --   6 (62.97 cM) 128357000 
    chicken
    (Gallus gallus)
    Aves C12orf326
    hypothetical protein LOC426083
    31(a)
    1 ↔ 1
    1(78760759-78764941)
    zebrafish
    (Danio rerio)
    Actinopterygii CU571169.56
    --
    24(a)
    1 ↔ 1
    4(5706066-5707847)


    ENSEMBL Gene Tree for RHNO1 (if available)
    TreeFam Gene Tree for RHNO1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/180 NCBI SNPs in RHNO1 are shown (see all 180    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1143730661,2
    F--2988464(+) TGTGCG/ACGGCC 7 -- int11Minor allele frequency- A:0.04WA 118
    rs1135697171,2
    C--2988493(+) TTGAAT/-ATTTT 7 -- int11Minor allele frequency- -:0.00CSA 2
    rs634617601,2
    C--2988494(+) TTTTTT/-CTTTT 7 -- int11Minor allele frequency- -:0.00NA 2
    rs1490925531,2
    --2988634(+) GCTGGA/GACTAC 7 -- int10--------
    rs124258991,2
    H--2988662(+) cccacA/Ctgatt 7 -- int10--------
    rs2004519131,2
    --2988778(+) TACAG-/GCGT  
            
    GAGCC
    7 -- int10--------
    rs2019730721,2
    --2988779(+) CAGGC-/TGTGAG 7 -- int10--------
    rs1852192091,2
    --2988975(+) ATAGAC/GTGAGA 7 -- int10--------
    rs1901483631,2
    --2988976(+) TAGAGA/TGAGAA 7 -- int10--------
    rs1431412481,2
    --2989009(+) GGGAAC/GGACTT 7 -- int10--------

    HapMap Linkage Disequilibrium report for RHNO1 (2985424 - 2998626 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for RHNO1: --

    SABiosciences Cancer Mutation PCR Assays
    Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RHNO1
    DNA2.0 Custom Variant and Variant Library Synthesis for RHNO1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RHNO1 for disorders           About GeneDecksing

    OMIM gene information: 614085    OMIM disorders: --

    3 diseases for RHNO1:    About MalaCards
    hepatoblastoma    breast cancer    prostatitis


    Export disorders for RHNO1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RHNO1 gene, integrated from 9 sources (see all 13):
    (articles sorted by number of sources associating them with RHNO1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A DNA damage response screen identifies RHINO, a 9-1- 1 and TopBP1 interacting protein required for ATR signaling. (PubMed id 21659603)1, 2, 3 Cotta-Ramusino C....Elledge S.J. (2011)
    2. Involvement of C12orf32 overexpression in breast carc inogenesis. (PubMed id 20811708)1, 2, 3 Kim J.W....Nakamura Y. (2010)
    3. Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas. (PubMed id 15221005)1, 2 Yamada S....Nakagawara A. (2004)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    6. Dynamics of cullin-RING ubiquitin ligase network revea led by systematic quantitative proteomics. (PubMed id 21145461)1 Bennett E.J....Harper J.W. (2010)
    7. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
    8. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. (PubMed id 17525332)1 Matsuoka S.... Elledge S.J. (2007)
    9. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
    10. The finished DNA sequence of human chromosome 12. (PubMed id 16541075)2 Scherer S.E.... Gibbs R.A. (2006)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 83695 HGNC: 28206 AceView: MGC13204 Ensembl:ENSG00000171792 euGenes: HUgn83695
    ECgene: RHNO1 H-InvDB: RHNO1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RHNO1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RHNO1 gene:
    Search GeneIP for patents involving RHNO1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
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     Browse OriGene Antibodies   OriGene shRNA RFP for RHNO1  
     OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RHNO1   OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RHNO1  
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     OriGene Custom Antibody Services for RHNO1   OriGene Custom Protein Services for RHNO1  
     OriGene Custom Immunoassay Development  

     
     
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RHNO1
     Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RHNO1
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     GenScript Custom Purified and Recombinant Proteins Services for RHNO1 GenScript Custom cDNA clones with any tag delivered in your preferred vector Services for RHNO1
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     Search Regulatory tfbs for RHNO1
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     SABiosciences Custom PCR Arrays for RHNO1
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     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
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     RHNO1 Proteins, Antibodies, CLIAs, and ELISAs
     Search LifeMap BioReagents cell lines for RHNO1
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     Protein Production and Purification
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     Search ThermoFisher Antibodies for RHNO1
     Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat RHNO1
     inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for RHNO1
    Sirion Biotech Customized:
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     inducible knockdown cell line services for RHNO1
     design and validation of potent shRNA sequences against RHNO1
     adenovirus for potent knockdown of RHNO1
     adenovirus for overexpression of RHNO1
     stable overexpressing cell line services for RHNO1
     inducible overexpressing cell line services for RHNO1
           
    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 14 May 2013

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