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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RHCG Gene

protein-coding   GIFtS: 53
GCID: GC15M090014

Rh family, C glycoprotein

(Previous names: chromosome 15 open reading frame 6, Rhesus blood group,...)
(Previous symbol: C15orf6)
 Explore 10 diseases affiliated with
RHCG via our new
 Human Malady Compendium 
Biological research products
for RHCG
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Rh Family, C Glycoprotein1 2     Rh Glycoprotein Kidney2 3
RHGK1 2 3 5     Rhesus Blood Group Family Type C Glycoprotein2 3
C15orf61 2 3     Tumor-Related Protein DRC22 3
PDRC21 2 3     Rh Type C Glycoprotein2 3
SLC42A31 2     Chromosome 15 Open Reading Frame 61
Rhesus Blood Group, C Glycoprotein1 2     Ammonium Transporter Rh Type C2
Rh Family Type C Glycoprotein2 3     CDRC23

External Ids:    HGNC: 181401   Entrez Gene: 514582   Ensembl: ENSG000001405197   OMIM: 6053815   UniProtKB: Q9UBD63   

Export aliases for RHCG gene to outside databases

Previous GC identifers: GC15M086419 GC15M083380 GC15M087601 GC15M087744 GC15M087815 GC15M066127


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RHCG_HUMAN, Q9UBD6
Function: Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia
secretion

Gene Wiki entry for RHCG


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000015.9  NC_018926.1  NT_010274.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RHCG gene promoter:
         AhR   MIF-1   Tal-1beta   Ik-3   E47   Arnt   MZF-1   IRF-7A   NF-Y   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRHCG promoter sequence
   Search SABiosciences Chromatin IP Primers for RHCG

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RHCG


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q25   Ensembl cytogenetic band:  15q26.1   HGNC cytogenetic band: 15q25

RHCG Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RHCG gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15M090014:  view genomic region     (about GC identifiers)

Start:
89,998,680 bp from pter      End:
90,039,844 bp from pter
Size:
41,165 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RHCG_HUMAN, Q9UBD6 (See protein sequence)
Recommended Name: Ammonium transporter Rh type C  
Size: 479 amino acids; 53179 Da
Subunit: Homotrimer
Subcellular location: Apical cell membrane; Multi-pass membrane protein. Note=Also detected at the basolateral membrane
and in subapical vesicles (By similarity)
Developmental stage: Specifically expressed in fetal kidney
1 PDB 3D structure from and Proteopedia for RHCG:
3HD6 (3D)    
Secondary accessions: A8K4D4 Q6X3Y4

Explore the universe of human proteins at neXtProt for RHCG: NX_Q9UBD6

Post-translational modifications:

  • N-glycosylated1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9UBD6

  • RHCG Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_057405.1  
    ENSEMBL proteins: 
     ENSP00000453588   ENSP00000268122   ENSP00000453496   ENSP00000452899   ENSP00000453716  
     ENSP00000438123  
    Reactome Protein details: Q9UBD6
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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for RHCG

    Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane TAS--
    GO:0005887integral to plasma membrane IDA10852913
    GO:0016020membrane ----
    GO:0016021integral to membrane ----
    GO:0016323basolateral plasma membrane IDA16928804


    RHCG for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RHCG for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR024041 NH4_transpt_AmtB-like_dom
     IPR002229 RhesusRHD

    Graphical View of Domain Structure for InterPro Entry Q9UBD6

    ProtoNet protein and cluster: Q9UBD6

    2 Blocks protein families:
    IPB002229 Rhesus blood group protein signature
    IPB010256 Rh-like protein/ammonium transporter


    UniProtKB/Swiss-Prot: RHCG_HUMAN, Q9UBD6
    Similarity: Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RHCG_HUMAN, Q9UBD6
    Function: Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia
    secretion

    miRNA
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    hsa-miR-596 hsa-miR-1184 hsa-miR-637
    SwitchGear 3'UTR luciferase reporter plasmidRHCG 3' UTR sequence
    Inhib. RNA
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RHCG

    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008519ammonium transmembrane transporter activity IGI11062476
    GO:0030506ankyrin binding IDA12719424
    GO:0051739ammonia transmembrane transporter activity IDA16580862


    RHCG for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Rhcgtm1Ibmm for RHCG
         5 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Rhcg):
     growth/size  homeostasis/metabolism  no phenotypic analysis  renal/urinary system  reproductive system 

    RHCG for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1SLC-mediated transmembrane transport
    SLC-mediated transmembrane transport1.00
    Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds0.38
    Transmembrane transport of small molecules0.50
    2Rhesus glycoproteins mediate ammonium transport.
    Rhesus glycoproteins mediate ammonium transport.1.00
    3Amine compound SLC transporters
    Amine compound SLC transporters1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    5        Reactome Pathways for RHCG
        Rhesus glycoproteins mediate ammonium transport.
    SLC-mediated transmembrane transport
    Transmembrane transport of small molecules
    Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds
    Amine compound SLC transporters



    RHCG for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RHCG

    Gene Ontology (GO): 5/9 biological process terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006873cellular ion homeostasis IDA14761968
    GO:0006885regulation of pH IEA--
    GO:0015696ammonium transport IGI11062476
    GO:0015837amine transport NAS10852913
    GO:0030855epithelial cell differentiation NAS12204676


    RHCG for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    RHCG for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RHCG

    1 HMDB Compound for RHCG    About this table
    CompoundSynonyms CAS #PubMed Ids
    AmmoniaNH3 (see all 31)7664-41-7--
    3 Novoseek chemical compound relationships for RHCG gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    amtb 90.9 1 16281947 (1)
    methylammonium 90.3 1 11062476 (1)
    ammonium 78.7 26 14761968 (5), 16580864 (3), 11062476 (3), 14656370 (2) (see all 9)

    Search CenterWatch for drugs/clinical trials and news about RHCG 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RHCG gene: 
    NM_016321.1  

    Unigene Cluster for RHCG:

    Rh family, C glycoprotein
    Hs.459284  [show with all ESTs]
    Unigene Representative Sequence: BC030965
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000560081(uc002boa.2) ENST00000268122(uc002bnz.2) ENST00000558360
    ENST00000558030 ENST00000558184(uc010bnq.1) ENST00000559638 ENST00000544600


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    hsa-miR-596 hsa-miR-1184 hsa-miR-637
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    Inhib. RNA
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    Primer
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    Additional cDNA sequence: 

    AF081497.1 AF193809.1 AK290899.1 AK298252.1 AK298641.1 AK301640.1 AK310222.1 AK313238.1 
    AY257182.1 BC015461.1 BC030965.1 

    6 DOTS entries:

    DT.206763  DT.65286547  DT.101979813  DT.100779178  DT.40216415  DT.91989395 

    24/80 AceView cDNA sequences (see all 80):

    CR597827 CB128022 BX284069 CR597837 BX356835 BX378267 AL603226 BI517523 
    AW772195 BC015461 CK300037 CA309850 BF353627 BI762408 BC030965 AI608603 
    CA310092 BI764566 BF354878 AI767967 AW085774 AI310728 U75825 AF081497 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RHCG expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GCTTCCTCGG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See RHCG Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RHCG

    SOURCE GeneReport for Unigene cluster: Hs.459284

    UniProtKB/Swiss-Prot: RHCG_HUMAN, Q9UBD6
    Tissue specificity: Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous
    epithelial tissues (at protein level). According to PubMed:11062476, specifically expressed in kidney

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for RHCG gene from 6/22 species (see all 22)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rhcg1 , 5 Rhesus blood group-associated C glycoprotein1, 5 81.42(n)1
    77.87(a)1
      7 (45.09 cM)5
    563151  NM_019799.31  NP_062773.21 
     795933635 
    chicken
    (Gallus gallus)
    Aves RHCG1 Rh family, C glycoprotein 73.32(n)
    75.39(a)
      415490  NM_001004370.1  NP_001004370.1 
    lizard
    (Anolis carolinensis)
    Reptilia RHCG6
    --
    70(a)
    1 ↔ 1
    GL343444.1(583928-632929)
    zebrafish
    (Danio rerio)
    Actinopterygii wufj85f072 wufj85f07 77.77(n)   337596  AF529360.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Rh503   -- 42(a)   64C4   --
    worm
    (Caenorhabditis elegans)
    Secernentea rhr-13 blood group RH(D) polypeptide 40(a)
    (best of 2)
      V(5428029-5430058)   --


    ENSEMBL Gene Tree for RHCG (if available)
    TreeFam Gene Tree for RHCG (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RHCG gene
    RHCE2  RHAG2  RHBG2  RHD2  
    10 SIMAP similar genes for RHCG using alignment to 6 protein entries:     RHCG_HUMAN (see all proteins):
    RH50    RHAG    Rh50    RHBG    RhDCw    RHD
    RHCE    RhIVb(J)    RhDTI    RhD

    RHCG for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/533 NCBI SNPs in RHCG are shown (see all 533    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1458186051,2
    --90014215(+) TCTACC/TCTCCT 1 -- int10--------
    rs169431831,2
    C,F,H,--90014227(+) AAACCT/ATACCC 1 -- int18Minor allele frequency- A:0.03NA NS EA 682
    rs71666061,2
    C,F,A,H,--90014333(+) CGCAGT/CCCTGC 1 -- int112Minor allele frequency- C:0.35NS EA NA WA CSA 787
    rs71618811,2
    C,F,A,H,--90014394(+) TTCTCA/CAGGGC 1 -- int114Minor allele frequency- C:0.17NA NS EA WA CSA 690
    rs169431881,2
    C,F,H,--90014494(+) CTCTAG/CTACCC 1 -- int18Minor allele frequency- C:0.03NA NS EA 682
    rs772591451,2
    --90014563(+) TGAAGA/GTAAGG 1 -- int10--------
    rs1929932661,2
    --90014620(+) GCCCCA/GTTGGA 1 -- int10--------
    rs1412900661,2
    --90014702(+) AGGTCC/TGGGGG 1 -- ut310--------
    rs115585911,2
    F,--90014748(-) TACCCC/TGTGCC 1 -- ut31 ese31Minor allele frequency- T:0.05EA 120
    rs1433108891,2
    --90014950(+) TCAGAC/TCCCCT 1 -- ut310--------

    HapMap Linkage Disequilibrium report for RHCG (89998680 - 90039844 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for RHCG
         1 CNV: 47910

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    DNA2.0 Custom Variant and Variant Library Synthesis for RHCG

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RHCG for disorders           About GeneDecksing

    OMIM gene information: 605381    OMIM disorders: --

    10 diseases for RHCG:    About MalaCards
    renal tubular acidosis    ovarian hyperstimulation syndrome    hemolytic anemia    squamous cell carcinoma
    anemia    astrocytoma    carcinoma    adenocarcinoma
    hepatitis    prostatitis

    4 diseases from the University of Copenhagen DISEASES database for RHCG:
    Metabolic acidosis     Ovarian hyperstimulation syndrome     Renal tubular acidosis     Infertility

    1 Novoseek disease relationship for RHCG gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    carcinoma squamous cell 11.3 1 12204676 (1)

    Human Genome Epidemiology (HuGE) Navigator: RHCG (4 documents)

    Export disorders for RHCG gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RHCG gene, integrated from 9 sources (see all 35):
    (articles sorted by number of sources associating them with RHCG)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of human RhCG and mouse Rhcg as novel nonerythroid Rh glycoprotein homologues predominantly expressed in kidney and testis. (PubMed id 10852913)1, 2, 3 Liu Z....Huang C.-H. (2000)
    2. NH3 is involved in the NH4+ transport induced by the functional expression of the human Rh C glycoprotein. (PubMed id 14761968)1, 2, 9 Bakouh N.... Planelles G. (2004)
    3. RhCG is downregulated in oesophageal squamous cell carcinomas, but expressed in multiple squamous epithelia. (PubMed id 12204676)1, 2, 9 Chen B.-S.... Wang M.-R. (2002)
    4. Function of human Rh based on structure of RhCG at 2. 1 A. (PubMed id 20457942)1, 2, 9 Gruswitz F....Stroud R.M. (2010)
    5. Ammonium transport properties of HEK293 cells expressing RhCG mutants: preliminary analysis of structure/function by site-directed mutagenesis. (PubMed id 16580862)1, 2, 9 Zidi-Yahiaoui N.... Mouro-Chanteloup I. (2006)
    6. The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. (PubMed id 11062476)1, 2, 9 Marini A.-M....Cherif-Zahar B. (2000)
    7. Structural involvement in substrate recognition of an essential aspartate residue conserved in Mep/Amt and Rh-type ammonium transporters. (PubMed id 16477434)1, 2, 9 Marini A.-M....Andre B. (2006)
    8. Human Rhesus B and Rhesus C glycoproteins: properties of facilitated ammonium transport in recombinant kidney cells. (PubMed id 15929723)1, 2 Zidi-Yahiaoui N.... Ripoche P. (2005)
    9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    10. RhCG is the major putative ammonia transporter expressed in the human kidney, and RhBG is not expressed at detectable levels. (PubMed id 19357182)1, 9 Brown A.C....Toye A.M. (2009)

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    Entrez Gene: 51458 HGNC: 18140 AceView: RHCG Ensembl:ENSG00000140519 euGenes: HUgn51458
    ECgene: RHCG H-InvDB: RHCG

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