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Aliases for RFWD3 Gene

Aliases for RFWD3 Gene

  • Ring Finger And WD Repeat Domain 3 2 3
  • RING Finger And WD Repeat Domain-Containing Protein 3 3 4
  • RING Finger Protein 201 3 4
  • RNF201 3 4
  • EC 6.3.2.- 4

External Ids for RFWD3 Gene

Previous GeneCards Identifiers for RFWD3 Gene

  • GC16M073214
  • GC16M060404

Summaries for RFWD3 Gene

GeneCards Summary for RFWD3 Gene

RFWD3 (Ring Finger And WD Repeat Domain 3) is a Protein Coding gene. GO annotations related to this gene include ligase activity and p53 binding.

UniProtKB/Swiss-Prot for RFWD3 Gene

  • E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RFWD3 Gene

Genomics for RFWD3 Gene

Regulatory Elements for RFWD3 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RFWD3 Gene

Chromosome:
16
Start:
74,621,394 bp from pter
End:
74,666,892 bp from pter
Size:
45,499 bases
Orientation:
Minus strand

Genomic View for RFWD3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RFWD3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RFWD3 Gene

Proteins for RFWD3 Gene

  • Protein details for RFWD3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q6PCD5-RFWD3_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RFWD3
    Protein Accession:
    Q6PCD5
    Secondary Accessions:
    • A8K585
    • B2RE35
    • D3DUJ8
    • Q5XKR3
    • Q9H9Q3
    • Q9NVT4

    Protein attributes for RFWD3 Gene

    Size:
    774 amino acids
    Molecular mass:
    85094 Da
    Quaternary structure:
    • Interacts with MDM2 and p53/TP53. Binds to the RPA complex via direct interaction with RPA2.
    SequenceCaution:
    • Sequence=BAA91662.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAF83889.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAG38132.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

neXtProt entry for RFWD3 Gene

Proteomics data for RFWD3 Gene at MOPED

Post-translational modifications for RFWD3 Gene

  • Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. Part of the phosphorylation depends upon RPA2 presence.
  • Ubiquitination at Lys 364, Lys 370, Lys 389, Lys 402, Lys 440, Lys 498, Lys 583, Lys 658, Lys 697, and Lys 766
  • Modification sites at PhosphoSitePlus

Other Protein References for RFWD3 Gene

Antibody Products

No data available for DME Specific Peptides for RFWD3 Gene

Domains & Families for RFWD3 Gene

Protein Domains for RFWD3 Gene

Suggested Antigen Peptide Sequences for RFWD3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q6PCD5

UniProtKB/Swiss-Prot:

RFWD3_HUMAN :
  • The coiled coil domain may be involved in RPA2-binding
  • Contains 1 RING-type zinc finger.
Domain:
  • The coiled coil domain may be involved in RPA2-binding
Similarity:
  • Contains 1 RING-type zinc finger.
  • Contains 3 WD repeats.
genes like me logo Genes that share domains with RFWD3: view

Function for RFWD3 Gene

Molecular function for RFWD3 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair.

Enzyme Numbers (IUBMB) for RFWD3 Gene

Gene Ontology (GO) - Molecular Function for RFWD3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002039 p53 binding IPI 20173098
GO:0004842 ubiquitin-protein transferase activity IDA 20173098
GO:0005515 protein binding IPI 19549727
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
genes like me logo Genes that share ontologies with RFWD3: view
genes like me logo Genes that share phenotypes with RFWD3: view

Animal Model Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RFWD3

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targets and HOMER Transcription for RFWD3 Gene

Localization for RFWD3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RFWD3 Gene

Nucleus. Nucleus, PML body. Cytoplasm. Note=In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies. In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair. This recruitment depends upon RPA2.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RFWD3 Gene COMPARTMENTS Subcellular localization image for RFWD3 gene
Compartment Confidence
nucleus 5

Gene Ontology (GO) - Cellular Components for RFWD3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 20173098
GO:0005737 cytoplasm IEA --
GO:0016605 PML body IEA --
GO:0035861 colocalizes_with site of double-strand break IDA 21504906
genes like me logo Genes that share ontologies with RFWD3: view

Pathways & Interactions for RFWD3 Gene

SuperPathways for RFWD3 Gene

No Data Available

UniProtKB/Swiss-Prot Q6PCD5-RFWD3_HUMAN

  • Pathway: Protein modification; protein ubiquitination

Gene Ontology (GO) - Biological Process for RFWD3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002230 positive regulation of defense response to virus by host IMP 22020285
GO:0006281 DNA repair IEA --
GO:0010212 response to ionizing radiation IDA 20173098
GO:0016567 protein ubiquitination IEA --
GO:0031571 mitotic G1 DNA damage checkpoint IMP 20173098
genes like me logo Genes that share ontologies with RFWD3: view

No data available for Pathways by source and SIGNOR curated interactions for RFWD3 Gene

Drugs & Compounds for RFWD3 Gene

(1) Drugs for RFWD3 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0

(1) Additional Compounds for RFWD3 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Antagonist, Gating inhibitor 58-64-0
genes like me logo Genes that share compounds with RFWD3: view

Transcripts for RFWD3 Gene

Unigene Clusters for RFWD3 Gene

Ring finger and WD repeat domain 3:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RFWD3

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RFWD3 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c · 5d · 5e ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12a · 12b ^
SP1: - - - - - - - -
SP2: -
SP3: - - -
SP4: - -
SP5: - - -
SP6: - -
SP7: -
SP8:
SP9:

ExUns: 13 ^ 14a · 14b ^ 15 ^ 16
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:

Relevant External Links for RFWD3 Gene

GeneLoc Exon Structure for
RFWD3
ECgene alternative splicing isoforms for
RFWD3

Expression for RFWD3 Gene

mRNA expression in normal human tissues for RFWD3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for RFWD3 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RFWD3 Gene



SOURCE GeneReport for Unigene cluster for RFWD3 Gene Hs.567525

genes like me logo Genes that share expression patterns with RFWD3: view

Protein tissue co-expression partners for RFWD3 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RFWD3 Gene

Orthologs for RFWD3 Gene

This gene was present in the common ancestor of animals.

Orthologs for RFWD3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RFWD3 35
  • 81.92 (n)
  • 76.07 (a)
RFWD3 36
  • 74 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RFWD3 35
  • 84.2 (n)
  • 77.79 (a)
RFWD3 36
  • 75 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rfwd3 35
  • 76.7 (n)
  • 70.03 (a)
Rfwd3 16
Rfwd3 36
  • 67 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RFWD3 35
  • 99.61 (n)
  • 99.22 (a)
RFWD3 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rfwd3 35
  • 74.22 (n)
  • 66.4 (a)
oppossum
(Monodelphis domestica)
Mammalia RFWD3 36
  • 60 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RFWD3 36
  • 60 (a)
OneToOne
chicken
(Gallus gallus)
Aves RFWD3 35
  • 67.27 (n)
  • 63.59 (a)
RFWD3 36
  • 55 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RFWD3 36
  • 56 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rfwd3 35
  • 60.66 (n)
  • 60.56 (a)
Str.818 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.29638 35
zebrafish
(Danio rerio)
Actinopterygii LOC100329327 35
  • 59.89 (n)
  • 59.15 (a)
rfwd3 36
  • 49 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005643 35
  • 41.44 (n)
  • 28.96 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG13025 35
  • 41.08 (n)
  • 30.45 (a)
CG13025 36
  • 26 (a)
OneToMany
CG13481 36
  • 23 (a)
OneToMany
CG14983 36
  • 25 (a)
OneToMany
CG17329 36
  • 27 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.787 36
  • 37 (a)
OneToOne
Species with no ortholog for RFWD3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RFWD3 Gene

ENSEMBL:
Gene Tree for RFWD3 (if available)
TreeFam:
Gene Tree for RFWD3 (if available)

Paralogs for RFWD3 Gene

No data available for Paralogs for RFWD3 Gene

Variants for RFWD3 Gene

Sequence variations from dbSNP and Humsavar for RFWD3 Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs1138860 -- 74,621,736(-) AGCCT(C/G)GGTGA utr-variant-3-prime
rs1138861 -- 74,621,703(-) AAAAA(A/C)AAACA utr-variant-3-prime
rs1802616 -- 74,668,314(+) TTGAC(A/C)AGAGC upstream-variant-2KB
rs3202910 -- 74,621,707(-) CAAAA(A/C)AAACA utr-variant-3-prime
rs3205212 -- 74,623,503(-) ACTAA(A/C)CATCG utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for RFWD3 Gene

Variant ID Type Subtype PubMed ID
nsv833279 CNV Loss 17160897
nsv906905 CNV Gain 21882294
esv275138 CNV Gain+Loss 21479260
nsv906906 CNV Gain 21882294

Variation tolerance for RFWD3 Gene

Residual Variation Intolerance Score: 13.25% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.66; 86.39% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RFWD3 Gene

HapMap Linkage Disequilibrium report
RFWD3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RFWD3 Gene

Disorders for RFWD3 Gene

Relevant External Links for RFWD3

Genetic Association Database (GAD)
RFWD3
Human Genome Epidemiology (HuGE) Navigator
RFWD3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RFWD3
genes like me logo Genes that share disorders with RFWD3: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RFWD3 Gene

Publications for RFWD3 Gene

  1. E3 ligase RFWD3 participates in replication checkpoint control. (PMID: 21504906) Gong Z. … Chen J. (J. Biol. Chem. 2011) 2 67
  2. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 67
  3. PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry. (PMID: 24332808) Marechal A. … Zou L. (Mol. Cell 2014) 67
  4. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. (PMID: 24639526) Carmon K.S. … Liu Q. (Proc. Natl. Acad. Sci. U.S.A. 2014) 67
  5. The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines. (PMID: 25260751) Charlaftis N. … Gallagher E. (EMBO J. 2014) 67

Products for RFWD3 Gene

Sources for RFWD3 Gene

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