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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

RFWD3 Gene

protein-coding GIFtS: 48
GCID: GC16M074656

ring finger and WD repeat domain 3

Explore 1 disease affiliated with
RFWD3 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Ring Finger And WD Repeat Domain 3¹ ²		FLJ10520¹
RNF201¹ ² ³		E3 Ubiquitin-Protein Ligase RFWD3²
RING Finger And WD Repeat Domain-Containing Protein 3² ³		EC 6.3.2.-³
RING Finger Protein 201² ³

External Ids:	HGNC: 25539¹	Entrez Gene: 55159²	Ensembl: ENSG00000168411⁷	OMIM: 614151⁵	UniProtKB: Q6PCD5³

Export aliases for RFWD3 gene to outside databases

Previous GC identifers: GC16M073214 GC16M060404

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RFWD3_HUMAN, Q6PCD5

Function: E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage,

and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by

catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In

response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2

from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and

repair

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000016.9 NC_018927.1 NT_010498.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the RFWD3 gene promoter:
NF-1 AML1a SRF (504 AA) Olf-1 GATA-1 GATA-6 Nkx6-1 NF-Y Hlf CBF(2)
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: RFWD3 promoter sequence

Search SABiosciences Chromatin IP Primers for RFWD3

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RFWD3

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q23.1 Ensembl cytogenetic band: 16q23.1 HGNC cytogenetic band: 16q22.3

RFWD3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RFWD3 gene location

GeneLoc information about chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16M074656: view genomic region (about GC identifiers)

Start:	74,655,292 bp from pter	End:	74,700,779 bp from pter
Size:	45,488 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RFWD3_HUMAN, Q6PCD5 (See protein sequence)

Recommended Name: E3 ubiquitin-protein ligase RFWD3

Size: 774 amino acids; 85094 Da

Subunit: Interacts with MDM2 and p53/TP53. Binds to the RPA complex via direct interaction with RPA2

Subcellular location: Nucleus. Nucleus, PML body. Cytoplasm. Note=In undamaged cells, found both in the cytoplasm and

in the nucleus, partially associated with PML nuclear bodies. In response to replication block, such as that caused by

hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to

stalled replication forks or to sites of DNA repair. This recruitment depends upon RPA2

Developmental stage: Up-regulated in the S-G2 phase

Sequence caution: Sequence=BAA91662.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Sequence=BAF83889.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAG38132.1;

Type=Erroneous initiation; Note=Translation N-terminally extended;

Secondary accessions: A8K585 B2RE35 D3DUJ8 Q5XKR3 Q9H9Q3 Q9NVT4

Explore the universe of human proteins at neXtProt for RFWD3: NX_Q6PCD5

Post-translational modifications:

Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA

damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize

p53/TP53. Part of the phosphorylation depends upon RPA2 presence¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q6PCD5

RFWD3 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_060594.3

ENSEMBL proteins:

ENSP00000354361 ENSP00000460049 ENSP00000459465 ENSP00000459899 ENSP00000459515

ENSP00000461285

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	Uscn Proteins for RFWD3

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005737	cytoplasm	IEA	--
GO:0016605	PML body	IEA	--

RFWD3 for ontologies About GeneDecksing

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	Uscn ELISAs and CLIAs for RFWD3

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

RFWD3 for domains About GeneDecksing

4 InterPro domains/families:

IPR001841 Znf_RING

IPR013083 Znf_RING/FYVE/PHD

IPR001680 WD40_repeat

IPR015943 WD40/YVTN_repeat-like_dom

Graphical View of Domain Structure for InterPro Entry Q6PCD5

ProtoNet protein and cluster: Q6PCD5

1 Blocks protein family: IPB001841 Zn-finger

UniProtKB/Swiss-Prot: RFWD3_HUMAN, Q6PCD5

Domain: The coiled coil domain may be involved in RPA2-binding

Similarity: Contains 1 RING-type zinc finger

Similarity: Contains 3 WD repeats

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: RFWD3_HUMAN, Q6PCD5

Function: E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage,

and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by

catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In

response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2

from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and

repair

Enzyme Number (IUBMB): EC 6.3.2.-¹

miRNA Products:		OriGene 3'-UTR Clone: RFWD3 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RFWD3
		8/26 QIAGEN miScript miRNA Assays for microRNAs that regulate RFWD3 (see all 26):
		hsa-miR-124* hsa-miR-142-3p hsa-miR-607 hsa-miR-539 hsa-miR-330-5p hsa-miR-2110 hsa-miR-371-5p hsa-miR-20b*

		SwitchGear 3'UTR luciferase reporter plasmid: RFWD3 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RFWD3 (see all 4) OriGene shRNA RFP: RFWD3 OriGene siRNA: RFWD3
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Gene Editing
Products:

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In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RFWD3

Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0002039	p53 binding	IPI	--
GO:0004842	ubiquitin-protein ligase activity	IDA	--
GO:0008270	zinc ion binding	IEA	--
GO:0070215	MDM2 binding	--	--
GO:0097371	MDM2/MDM4 family protein binding	IPI	--

RFWD3 for ontologies About GeneDecksing

2 GenomeRNAi human phenotypes for RFWD3:

Decreased viability with cispl

Increased gamma-H2AX phosphory

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

UniProtKB/Swiss-Prot: RFWD3_HUMAN, Q6PCD5

Pathway: Protein modification; protein ubiquitination

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for RFWD3

STRING Interaction Network Preview (showing 5 interactants - click image to see 15)

5/17 Interacting proteins for RFWD3 (Q6PCD5³ ENSP00000354361⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 17)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
UBE2D2	P62837³, ENSP00000381717⁴	I2D: score=2 STRING: ENSP00000381717
UBE2D3	P61077³, ENSP00000349722⁴	I2D: score=2 STRING: ENSP00000349722
UBE2N	P61088³, ENSP00000316176⁴	I2D: score=2 STRING: ENSP00000316176
UBE2W	Q96B02³, ENSP00000397453⁴	I2D: score=2 STRING: ENSP00000397453
MDM2	Q00987³, ENSP00000417281⁴	I2D: score=1 STRING: ENSP00000417281

About this table

Gene Ontology (GO): 4 biological process terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006281	DNA repair	IEA	--
GO:0010212	response to ionizing radiation	IDA	--
GO:0016567	protein ubiquitination	IDA	--
GO:0031571	mitotic cell cycle G1/S transition DNA damage checkpoint	IMP	--

RFWD3 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for RFWD3

2 HMDB Compounds for RFWD3 About this table

Compound	Synonyms	CAS #	PubMed Ids
ADP	adenosindiphosphorsaeure (see all 8)	58-64-0	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--

Search CenterWatch for drugs/clinical trials and news about RFWD3

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for RFWD3 gene:

NM_018124.3

Unigene Cluster for RFWD3:

Ring finger and WD repeat domain 3

Hs.567525 [show with all ESTs]

Unigene Representative Sequence: NM_018124

12 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000361070(uc010vmx.2 uc002fda.3 uc010cgq.3) ENST00000571750

ENST00000575154 ENST00000575281 ENST00000572610 ENST00000572337 ENST00000571776

ENST00000575397 ENST00000572990 ENST00000576652 ENST00000575113 ENST00000572840

miRNA Products:		OriGene 3'-UTR Clone: RFWD3 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RFWD3
		8/26 QIAGEN miScript miRNA Assays for microRNAs that regulate RFWD3 (see all 26):
		hsa-miR-124* hsa-miR-142-3p hsa-miR-607 hsa-miR-539 hsa-miR-330-5p hsa-miR-2110 hsa-miR-371-5p hsa-miR-20b*

		SwitchGear 3'UTR luciferase reporter plasmid: RFWD3 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RFWD3 (see all 4) OriGene shRNA RFP: RFWD3 OriGene siRNA: RFWD3
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RFWD3

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RFWD3 (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RFWD3 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
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		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RFWD3
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RFWD3

Additional cDNA sequence:

AK001382.1 AK022673.1 AK291200.1 AK296727.1 AK315786.1 BC002574.2 BC059371.1

13 DOTS entries:

DT.208381 DT.91975406 DT.101983212 DT.100018510 DT.97790418 DT.100713831 DT.120664034 DT.91747005

DT.120663993 DT.97806907 DT.95352376 DT.97808222 DT.120664046

24/175 AceView cDNA sequences (see all 175):

BQ677500 CR598867 BE740107 BQ221851 AU127701 BM560829 CB151897 BP872280

BX955838 AU124886 AI560910 BQ649632 CK905473 BC002574 AA888009 CD516080

BC059371 BX641846 BE733156 BQ215818 BM913008 AI299008 AA227251 BI063493

GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for RFWD3 (see all 9) About this scheme

ExUns:	1a	·	1b	·	1c	·	1d	^	2	^	3	^	4a	·	4b	^	5a	·	5b	·	5c	·	5d	·	5e	^	6a	·	6b	^	7a	·	7b	^	8a	·	8b	^	9a	·	9b	^	10	^	11a	·	11b	^	12a	·	12b	^
SP1:									-		-		-		-										-						-										-								-
SP2:																																									-
SP3:									-		-														-
SP4:									-						-
SP5:									-		-				-

ExUns:	13	^	14a	·	14b	^	15	^	16
SP1:
SP2:
SP3:
SP4:
SP5:

ECgene alternative splicing isoforms for RFWD3

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

RFWD3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GGAACTCTGT

About this image

RFWD3 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

2 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Heart

Atrioventricular Canal

Atrioventricular Canal Cells

Myocardium

Heart

Outflow Tract

Heart

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See RFWD3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RFWD3

SOURCE GeneReport for Unigene cluster: Hs.567525

SABiosciences Custom PCR Arrays for RFWD3

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		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RFWD3

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RFWD3

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of eukaryotes.

Orthologs for RFWD3 gene from 8/22 species (see all 22) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Rfwd3^{1 , 5}	ring finger and WD repeat domain 3^{1, 5}	76.73(n)¹ 69.4(a)¹		8 (57.98 cM)⁵ 234736¹ NM_146218.4¹ NP_666330.2¹ 111270944⁵
chicken (Gallus gallus)	Aves	RFWD3¹	ring finger and WD repeat domain 3	70.56(n) 68.39(a)		415684 XM_414051.3 XP_414051.2
lizard (Anolis carolinensis)	Reptilia	RFWD3⁶	--	63(a)	1 ↔ 1	GL343840.1(186066-195143)
African clawed frog (Xenopus laevis)	Amphibia	Xl.29638²	Xenopus laevis transcribed sequences	73.21(n)		BJ062741.1
zebrafish (Danio rerio)	Actinopterygii	57078557²	--	71.47(n)		57078557
fruit fly (Drosophila melanogaster)	Insecta	CG13025¹	CG13025	40.86(n) 29.96(a)		39874 NM_140662.3 NP_648919.1
thale cress (Arabidopsis thaliana)	eudicotyledons	AT5G03450¹	transducin/WD40 domain-containing protein	40.93(n) 26.93(a)		831835 NM_120424.3 NP_195965.2
rice (Oryza sativa)	Liliopsida	Os03g0108200¹	hypothetical protein	--		9271783 NM_001186302.1 NP_001173231.1

ENSEMBL Gene Tree for RFWD3 (if available)
TreeFam Gene Tree for RFWD3 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 16 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs36009591^1,2	C	--	74654875(+)	`CAGTT-/AAAAAA`	1	--	ds500¹		2		NA	4
rs28711408^1,2	C	--	74654888(+)	`AAAAAG/AAAAAA`	1	--	ds500¹		2		NA	4
rs4887780^1,2	C,F,	--	74654889(+)	`AAAAAG/AAAAAA`	1	--	ds500¹		2		NA WA	4
rs184940789^1,2		--	74654956(+)	`GCAGAG/TCACAA`	1	--	ds500¹		0	--	--	--	--
rs189010602^1,2		--	74655107(+)	`GAGGCA/GGAGTT`	1	--	ds500¹		0	--	--	--	--
rs140344281^1,2		--	74655127(+)	`CCAAGA/TTGGCG`	1	--	ds500¹		0	--	--	--	--
rs4887781^1,2	C,F,	--	74655150(+)	`AGCTAG/CGGCTA`	1	--	ds500¹		7		NA WA CSA EA	365
rs78484755^1,2	C,F,	--	74655179(+)	`CAAAAA/CACAAA`	1	--	ds500¹		5		NA WA EA	362
rs150363088^1,2		--	74655268(+)	`AAGTGA/GAAGAG`	1	--	ds500¹		0	--	--	--	--
rs138209252^1,2		--	74655436(+)	`AGTTCA/GTTTAA`	1	--	ut3¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for RFWD3 (74655292 - 74700779 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for RFWD3: --

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RFWD3

DNA2.0 Custom Variant and Variant Library Synthesis for RFWD3

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

RFWD3 for disorders           About GeneDecksing

OMIM gene information: 614151    OMIM disorders: --

1 disease for RFWD3:    About MalaCards

cholesterol

Human Genome Epidemiology (HuGE) Navigator: RFWD3 (1 document)

Export disorders for RFWD3 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for RFWD3 gene, integrated from 9 sources (see all 20):
(articles sorted by number of sources associating them with RFWD3)

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E3 ligase RFWD3 participates in replication checkpoin t control. (PubMed id 21504906)^{1, 2, 3} Gong Z. and Chen J. (2011)
RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response. (PubMed id 21558276)^{1, 2} Liu S.... Wang Y. (2011)
RFWD3-Mdm2 ubiquitin ligase complex positively regula tes p53 stability in response to DNA damage. (PubMed id 20173098)^{1, 2} Fu X....Wang Y. (2010)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)¹ Udeshi N.D....Carr S.A. (2012)
A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)¹ Wagner S.A....Choudhary C. (2011)
Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)¹ Kim W....Gygi S.P. (2011)
Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)¹ Lee K.A....Doedens J.R. (2011)
Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)¹ OlA!h J....OvA!di J. (2011)

(in PubMed, OMIM, and NCBI Bookshelf)
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 55159	HGNC: 25539	AceView: FLJ10520	Ensembl:ENSG00000168411	euGenes: HUgn55159
ECgene: RFWD3	H-InvDB: RFWD3

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for RFWD3	Pharmacogenomics, SNPs, Pathways

Intellectual Property for RFWD3 gene
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Patent Information for RFWD3 gene:
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ring finger and WD repeat domain 3

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