Free for academic non-profit institutions. Other users need a Commercial license

Aliases for RFK Gene

Aliases for RFK Gene

  • Riboflavin Kinase 2 3
  • ATP:Riboflavin 5-Phosphotransferase 3 4
  • Flavokinase 3 4
  • 0610038L10Rik 3
  • EC 2.7.1.26 4
  • RIFK 3

External Ids for RFK Gene

Previous GeneCards Identifiers for RFK Gene

  • GC09M074458
  • GC09M076230
  • GC09M078190
  • GC09M079000
  • GC09M048829

Summaries for RFK Gene

Entrez Gene Summary for RFK Gene

  • Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]

GeneCards Summary for RFK Gene

RFK (Riboflavin Kinase) is a Protein Coding gene. Diseases associated with RFK include riboflavin deficiency. Among its related pathways are Disease and Metabolism. GO annotations related to this gene include riboflavin kinase activity.

UniProtKB/Swiss-Prot for RFK Gene

  • Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RFK Gene

Genomics for RFK Gene

Regulatory Elements for RFK Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RFK Gene

Start:
76,385,517 bp from pter
End:
76,394,528 bp from pter
Size:
9,012 bases
Orientation:
Minus strand

Genomic View for RFK Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RFK Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RFK Gene

Proteins for RFK Gene

  • Protein details for RFK Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q969G6-RIFK_HUMAN
    Recommended name:
    Riboflavin kinase
    Protein Accession:
    Q969G6
    Secondary Accessions:
    • Q5JSG9
    • Q9NUT7

    Protein attributes for RFK Gene

    Size:
    155 amino acids
    Molecular mass:
    17623 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Zinc or magnesium.;
    Quaternary structure:
    • Monomer. Directly interacts with TNFRSF1A death domain. TNFRSF1A-binding may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA.
    SequenceCaution:
    • Sequence=AAH07069.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=BAA92033.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RFK Gene

neXtProt entry for RFK Gene

Proteomics data for RFK Gene at MOPED

Post-translational modifications for RFK Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for RFK Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for RFK Gene

Domains for RFK Gene

Protein Domains for RFK Gene

Suggested Antigen Peptide Sequences for RFK Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with RFK: view

No data available for Gene Families and UniProtKB/Swiss-Prot for RFK Gene

Function for RFK Gene

Molecular function for RFK Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + riboflavin = ADP + FMN
UniProtKB/Swiss-Prot Function: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.

Enzyme Numbers (IUBMB) for RFK Gene

Gene Ontology (GO) - Molecular Function for RFK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding --
GO:0005524 ATP binding IEA --
GO:0008531 riboflavin kinase activity TAS --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with RFK: view

Phenotypes for RFK Gene

genes like me logo Genes that share phenotypes with RFK: view

Animal Models for RFK Gene

MGI Knock Outs for RFK:
  • Rfk tm1.2Jcbr

miRNA for RFK Gene

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RFK

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for RFK Gene

Localization for RFK Gene

Subcellular locations from UniProtKB/Swiss-Prot for RFK Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RFK Gene COMPARTMENTS Subcellular localization image for RFK gene
Compartment Confidence
cytosol 4
mitochondrion 2
nucleus 2
extracellular 1

Gene Ontology (GO) - Cellular Components for RFK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm NAS 12623014
GO:0005739 mitochondrion IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with RFK: view

Pathways for RFK Gene

SuperPathways for RFK Gene

genes like me logo Genes that share pathways with RFK: view

UniProtKB/Swiss-Prot Q969G6-RIFK_HUMAN

  • Pathway: Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1

Gene Ontology (GO) - Biological Process for RFK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006766 vitamin metabolic process TAS --
GO:0006767 water-soluble vitamin metabolic process TAS --
GO:0006771 riboflavin metabolic process TAS --
GO:0006915 apoptotic process IMP 19641494
GO:0009231 riboflavin biosynthetic process NAS 12623014
genes like me logo Genes that share ontologies with RFK: view

Compounds for RFK Gene

(5) HMDB Compounds for RFK Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Flavin Mononucleotide
  • FMN
146-17-8
Magnesium
  • Magnesium
7439-95-4
Riboflavin
  • (-)-Riboflavin
83-88-5

(3) Drugbank Compounds for RFK Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
Adenosine-5'-Diphosphate
20398-34-9 target
Riboflavin
  • Lactoflavin
83-88-5 target ligand
Riboflavin Monophosphate
146-17-8 target

(6) Novoseek inferred chemical compound relationships for RFK Gene

Compound -log(P) Hits PubMed IDs
riboflavin 85.5 12
flavin mononucleotide 83.4 2
flavin-adenine dinucleotide 82 1
flavin 76.4 3
mgadp 73 1

(2) PharmGKB related drug/compound annotations for RFK Gene

Drug/compound Annotation
citalopram CA
escitalopram CA
genes like me logo Genes that share compounds with RFK: view

Transcripts for RFK Gene

Unigene Clusters for RFK Gene

Riboflavin kinase:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RFK

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RFK Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c · 7d
SP1: - -
SP2: - - -
SP3: - -
SP4: -

Relevant External Links for RFK Gene

GeneLoc Exon Structure for
RFK
ECgene alternative splicing isoforms for
RFK

Expression for RFK Gene

mRNA expression in normal human tissues for RFK Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for RFK Gene

SOURCE GeneReport for Unigene cluster for RFK Gene Hs.37558

mRNA Expression by UniProt/SwissProt for RFK Gene

Q969G6-RIFK_HUMAN
Tissue specificity: Detected in brain, placenta and urinary bladder
genes like me logo Genes that share expressions with RFK: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for RFK Gene

Orthologs for RFK Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RFK Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RFK 35
  • 99.74 (n)
  • 99.22 (a)
RFK 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RFK 35
  • 93.76 (n)
  • 94.84 (a)
RFK 36
  • 63 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RFK 35
  • 92.9 (n)
  • 95.48 (a)
RFK 36
  • 39 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rfk 35
  • 89.25 (n)
  • 93.55 (a)
Rfk 16
Rfk 36
  • 94 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 72 (a)
OneToMany
-- 36
  • 50 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia RFK 36
  • 54 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rfk 35
  • 90.32 (n)
  • 93.55 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 69 (a)
OneToMany
RFK 35
  • 80.26 (n)
  • 80.26 (a)
RFK 36
  • 78 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia RFK 36
  • 73 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.15775 35
tropical clawed frog
(Silurana tropicalis)
Amphibia rfk 35
  • 72.94 (n)
  • 73.38 (a)
Str.3084 35
zebrafish
(Danio rerio)
Actinopterygii rfk 35
  • 69.48 (n)
  • 75.97 (a)
rfk 36
  • 70 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG2846 35
  • 62.73 (n)
  • 61.81 (a)
CG2846 36
  • 58 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea R10H10.6 35
  • 56.17 (n)
  • 48.82 (a)
R10H10.6 36
  • 46 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ABL109W 35
  • 54.13 (n)
  • 47.2 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes FMN1 35
  • 54.26 (n)
  • 47.29 (a)
FMN1 36
  • 27 (a)
OneToOne
FMN1 38
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C15213g 35
  • 54.86 (n)
  • 47.24 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons FMN/FHY 35
  • 56.43 (n)
  • 50.71 (a)
rice
(Oryza sativa)
Liliopsida Os10g0464400 35
  • 53.85 (n)
  • 49.65 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU03970 35
  • 51.18 (n)
  • 48.82 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes fmn1 35
  • 52.72 (n)
  • 48.23 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.7996 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.1889 36
  • 51 (a)
OneToOne
Species with no ortholog for RFK:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RFK Gene

ENSEMBL:
Gene Tree for RFK (if available)
TreeFam:
Gene Tree for RFK (if available)

Paralogs for RFK Gene

genes like me logo Genes that share paralogs with RFK: view

No data available for Paralogs for RFK Gene

Variants for RFK Gene

Sequence variations from dbSNP and Humsavar for RFK Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type MAF
rs1129399 -- 76,386,988(-) GCAGT(G/T)GCTCA utr-variant-3-prime
rs2251604 -- 76,392,895(-) ACAGA(A/G)TCTGG intron-variant
rs2490579 -- 76,387,859(+) CGGGG(C/T)GTGAT intron-variant
rs2490580 -- 76,388,865(+) AGGTG(A/G)CAGTG intron-variant
rs2490581 -- 76,393,368(+) TGCCC(A/G)GCTAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RFK Gene

Variant ID Type Subtype PubMed ID
nsv893478 CNV Gain 21882294
nsv893479 CNV Gain 21882294
nsv6570 CNV Insertion 18451855

Relevant External Links for RFK Gene

HapMap Linkage Disequilibrium report
RFK

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RFK Gene

Disorders for RFK Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for RFK Gene including...

Search for RFK Gene in MalaCards »

Relevant External Links for RFK

Genetic Association Database (GAD)
RFK
Human Genome Epidemiology (HuGE) Navigator
RFK
genes like me logo Genes that share disorders with RFK: view

No data available for UniProtKB/Swiss-Prot for RFK Gene

Publications for RFK Gene

  1. Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch. (PMID: 12623014) Karthikeyan S. … Zhang H. (Structure 2003) 3 4 23
  2. Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism. (PMID: 14580199) Karthikeyan S. … Zhang H. (Biochemistry 2003) 2 3 23
  3. Riboflavin kinase couples TNF receptor 1 to NADPH oxidase. (PMID: 19641494) Yazdanpanah B. … Kroenke M. (Nature 2009) 3 4 23
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4

Products for RFK Gene

  • Addgene plasmids for RFK

Sources for RFK Gene

Back to Top

Content