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Aliases for RFC1 Gene

Aliases for RFC1 Gene

  • Replication Factor C (Activator 1) 1, 145kDa 2 3
  • Replication Factor C 140 KDa Subunit 3 4
  • Replication Factor C Large Subunit 3 4
  • Activator 1 140 KDa Subunit 3 4
  • Activator 1 Large Subunit 3 4
  • DNA-Binding Protein PO-GA 3 4
  • Activator 1 Subunit 1 3 4
  • RF-C 140 KDa Subunit 3 4
  • A1 140 KDa Subunit 3 4
  • RFC140 3 4
  • RECC1 3 6
  • Replication Factor C (Activator 1) 1 (145kD) 2
  • Replication Factor C Subunit 1 3
  • MHC Binding Factor, Beta 3
  • Replication Factor C1 3
  • MHCBFB 3
  • PO-GA 3
  • RFC 3
  • A1 3

External Ids for RFC1 Gene

Previous GeneCards Identifiers for RFC1 Gene

  • GC04M039463
  • GC04M039123
  • GC04M039186
  • GC04M039111
  • GC04M038965
  • GC04M038615

Summaries for RFC1 Gene

Entrez Gene Summary for RFC1 Gene

  • This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]

GeneCards Summary for RFC1 Gene

RFC1 (Replication Factor C (Activator 1) 1, 145kDa) is a Protein Coding gene. Diseases associated with RFC1 include pediatric osteosarcoma and hutchinson-gilford progeria. Among its related pathways are Cell Cycle, Mitotic and Cell Cycle, Mitotic. GO annotations related to this gene include enzyme activator activity and DNA clamp loader activity.

UniProtKB/Swiss-Prot for RFC1 Gene

  • The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.

  • Interacts with C-terminus of PCNA. 5 phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.

Gene Wiki entry for RFC1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RFC1 Gene

Genomics for RFC1 Gene

Regulatory Elements for RFC1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RFC1 Gene

Start:
39,287,449 bp from pter
End:
39,366,381 bp from pter
Size:
78,933 bases
Orientation:
Minus strand

Genomic View for RFC1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RFC1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RFC1 Gene

Proteins for RFC1 Gene

  • Protein details for RFC1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P35251-RFC1_HUMAN
    Recommended name:
    Replication factor C subunit 1
    Protein Accession:
    P35251
    Secondary Accessions:
    • A8K6E7
    • Q5XKF5
    • Q6PKU0
    • Q86V41
    • Q86V46

    Protein attributes for RFC1 Gene

    Size:
    1148 amino acids
    Molecular mass:
    128255 Da
    Quaternary structure:
    • Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

    Three dimensional structures from OCA and Proteopedia for RFC1 Gene

    Alternative splice isoforms for RFC1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RFC1 Gene

Proteomics data for RFC1 Gene at MOPED

Post-translational modifications for RFC1 Gene

  • Ubiquitination at Lys243, Lys468, Lys551, Lys556, Lys645, and Lys700
  • Modification sites at PhosphoSitePlus

Other Protein References for RFC1 Gene

Antibody Products

No data available for DME Specific Peptides for RFC1 Gene

Domains for RFC1 Gene

Gene Families for RFC1 Gene

HGNC:
  • AATP :ATPases / AAA-type

Suggested Antigen Peptide Sequences for RFC1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P35251

UniProtKB/Swiss-Prot:

RFC1_HUMAN :
  • P35251
Domain:
  • Contains 1 BRCT domain.
Family:
  • Belongs to the activator 1 large subunit family.
genes like me logo Genes that share domains with RFC1: view

Function for RFC1 Gene

Molecular function for RFC1 Gene

GENATLAS Biochemistry: replication factor C1,component of complex A-1,DNA polymerase accessory protein "clamp loader",ATP dependent,required to assemble PCNA and polymerase delta on the DNA template
UniProtKB/Swiss-Prot Function: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
UniProtKB/Swiss-Prot Function: Interacts with C-terminus of PCNA. 5 phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.

Gene Ontology (GO) - Molecular Function for RFC1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IBA --
GO:0003689 DNA clamp loader activity IEA --
GO:0003690 double-stranded DNA binding IEA --
GO:0005515 protein binding IPI 15655353
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with RFC1: view

Animal Model Products

CRISPR Products

miRNA for RFC1 Gene

miRTarBase miRNAs that target RFC1

No data available for Enzyme Numbers (IUBMB) , Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for RFC1 Gene

Localization for RFC1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RFC1 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RFC1 Gene COMPARTMENTS Subcellular localization image for RFC1 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for RFC1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm TAS --
GO:0005663 DNA replication factor C complex IDA 9488738
GO:0005730 nucleolus IEA --
GO:0005737 cytoplasm --
genes like me logo Genes that share ontologies with RFC1: view

Pathways for RFC1 Gene

genes like me logo Genes that share pathways with RFC1: view

PCR Array Products

Gene Ontology (GO) - Biological Process for RFC1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0000278 mitotic cell cycle TAS --
GO:0000722 telomere maintenance via recombination TAS --
GO:0000723 telomere maintenance TAS --
GO:0006200 obsolete ATP catabolic process --
genes like me logo Genes that share ontologies with RFC1: view

Compounds for RFC1 Gene

(6) Novoseek inferred chemical compound relationships for RFC1 Gene

Compound -log(P) Hits PubMed IDs
phosphocellulose 49.3 2
atp 47.3 25
atpgammas 36.7 3
alanine 3.67 2
adp 0 1
genes like me logo Genes that share compounds with RFC1: view

Transcripts for RFC1 Gene

Unigene Clusters for RFC1 Gene

Replication factor C (activator 1) 1, 145kDa:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RFC1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RFC1 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b ^ 15a · 15b · 15c ^ 16 ^
SP1: - -
SP2: -
SP3: - -
SP4:
SP5: -
SP6: -
SP7:
SP8: -
SP9:
SP10:
SP11:
SP12:
SP13:

ExUns: 17a · 17b ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25 ^ 26 ^ 27a · 27b ^ 28a · 28b
SP1:
SP2:
SP3:
SP4: -
SP5:
SP6:
SP7:
SP8:
SP9:
SP10: -
SP11:
SP12:
SP13:

Relevant External Links for RFC1 Gene

GeneLoc Exon Structure for
RFC1
ECgene alternative splicing isoforms for
RFC1

Expression for RFC1 Gene

mRNA expression in normal human tissues for RFC1 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RFC1 Gene

SOURCE GeneReport for Unigene cluster for RFC1 Gene Hs.507475

mRNA Expression by UniProt/SwissProt for RFC1 Gene

P35251-RFC1_HUMAN
Tissue specificity: Wide tissue distribution. Undetectable in placental tissue
genes like me logo Genes that share expressions with RFC1: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for RFC1 Gene

Orthologs for RFC1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RFC1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RFC1 35
  • 99.51 (n)
  • 99.65 (a)
RFC1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia RFC1 35
  • 88.93 (n)
  • 86.23 (a)
RFC1 36
  • 93 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RFC1 35
  • 90.48 (n)
  • 89.44 (a)
RFC1 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rfc1 35
  • 83.81 (n)
  • 83.57 (a)
Rfc1 16
Rfc1 36
  • 83 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RFC1 36
  • 77 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RFC1 36
  • 72 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rfc1 35
  • 83.5 (n)
  • 82.47 (a)
chicken
(Gallus gallus)
Aves RFC1 35
  • 72.15 (n)
  • 70.91 (a)
RFC1 36
  • 69 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RFC1 36
  • 64 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rfc1 35
  • 66.39 (n)
  • 66.54 (a)
zebrafish
(Danio rerio)
Actinopterygii rfc1 35
  • 62.15 (n)
  • 62.43 (a)
rfc1 36
  • 56 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP001290 35
  • 49.89 (n)
  • 46.1 (a)
fruit fly
(Drosophila melanogaster)
Insecta Gnf1 35
  • 51.03 (n)
  • 48.48 (a)
Gnf1 36
  • 39 (a)
OneToOne
Gnf1 37
  • 42 (a)
worm
(Caenorhabditis elegans)
Secernentea rfc-1 36
  • 36 (a)
OneToOne
rfc-1 37
  • 42 (a)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ACR102W 35
  • 46.88 (n)
  • 38.34 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RFC1 35
  • 48.9 (n)
  • 41.03 (a)
RFC1 36
  • 34 (a)
OneToOne
RFC1 38
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0D06897g 35
  • 48.01 (n)
  • 36.8 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU06767 35
  • 47.85 (n)
  • 39.88 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rfc1 35
  • 49.04 (n)
  • 38.34 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 49 (a)
OneToOne
Species with no ortholog for RFC1:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RFC1 Gene

ENSEMBL:
Gene Tree for RFC1 (if available)
TreeFam:
Gene Tree for RFC1 (if available)

Paralogs for RFC1 Gene

Selected SIMAP similar genes for RFC1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with RFC1: view

No data available for Paralogs for RFC1 Gene

Variants for RFC1 Gene

Sequence variations from dbSNP and Humsavar for RFC1 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs9825 -- 39,287,727(+) CGGCC(A/G)CTCAA utr-variant-3-prime
rs17754 -- 39,287,688(+) AGCTA(C/G)ACATC utr-variant-3-prime
rs900562 -- 39,330,044(+) aaaaa(A/T)TTTTT intron-variant
rs921494 -- 39,327,250(+) AGTTT(C/G)CATTC intron-variant
rs957426 -- 39,310,246(-) TTCAG(A/T)CTTTC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RFC1 Gene

Variant ID Type Subtype PubMed ID
nsv878940 CNV Gain 21882294
nsv878941 CNV Gain 21882294

Relevant External Links for RFC1 Gene

HapMap Linkage Disequilibrium report
RFC1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RFC1 Gene

Disorders for RFC1 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for RFC1 Gene including...

Search for RFC1 Gene in MalaCards »

(3) Novoseek inferred disease relationships for RFC1 Gene

Disease -log(P) Hits PubMed IDs
retinoblastoma 26 3
leukemia 0 1
tumors 0 4

Relevant External Links for RFC1

Genetic Association Database (GAD)
RFC1
Human Genome Epidemiology (HuGE) Navigator
RFC1
genes like me logo Genes that share disorders with RFC1: view

No data available for UniProtKB/Swiss-Prot for RFC1 Gene

Publications for RFC1 Gene

  1. cDNAs encoding the large subunit of human replication factor C. (PMID: 8248204) Bunz F. … Stillman B. (Proc. Natl. Acad. Sci. U.S.A. 1993) 3 4 23
  2. Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen. (PMID: 8999859) Mossi R. … Huebscher U. (J. Biol. Chem. 1997) 3 4 23
  3. A conserved domain of the large subunit of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells. (PMID: 8861969) Fotedar R. … Fotedar A. (EMBO J. 1996) 3 23
  4. Deletion analysis of the large subunit p140 in human replication factor C reveals regions required for complex formation and replication activities. (PMID: 9092549) Uhlmann F. … Hurwitz J. (J. Biol. Chem. 1997) 3 23
  5. The large subunit of replication factor C is a substrate for caspase-3 in vitro and is cleaved by a caspase-3-like protease during Fas-mediated apoptosis. (PMID: 9351817) RhAcaume E. … Denis F. (EMBO J. 1997) 3 23

Products for RFC1 Gene

Sources for RFC1 Gene

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