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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

REG4 Gene

protein-coding   GIFtS: 51
GCID: GC01M120336

regenerating islet-derived family, member 4

 Explore 22 diseases affiliated with
REG4 via our new
 Human Malady Compendium 
Biological research products
for REG4
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Regenerating Islet-Derived Family, Member 41 2     Regenerating Islet-Derived Protein IV2 3
GISP1 2 3     REG-42 3
RELP1 2 3     REG-Like Protein2 3
REG-IV1 2     Regenerating Gene Type IV2
Gastrointestinal Secretory Protein2 3     Regenerating Islet-Derived Protein 42
Reg IV2 3     

External Ids:    HGNC: 229771   Entrez Gene: 839982   Ensembl: ENSG000001341937   OMIM: 6098465   UniProtKB: Q9BYZ83   

Export aliases for REG4 gene to outside databases

Previous GC identifers: GC01M119483 GC01M119683 GC01M120048 GC01M120138 GC01M118195


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: REG4_HUMAN, Q9BYZ8
Function: Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate
recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the
gastrointestinal epithelium

Gene Wiki entry for REG4


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_032977.9  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the REG4 gene promoter:
         LHX3b/Lhx3b   AML1a   AP-1   ATF-2   FOXO4   FOXJ2 (long isoform)   Cart-1   LHX3a/Lhx3a   c-Jun   FOXJ2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidREG4 promoter sequence
   Search SABiosciences Chromatin IP Primers for REG4

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat REG4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p13.1-p12   Ensembl cytogenetic band:  1p12   HGNC cytogenetic band: 1p13.1-p12

REG4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
REG4 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M120336:  view genomic region     (about GC identifiers)

Start:
120,336,641 bp from pter      End:
120,354,283 bp from pter
Size:
17,643 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: REG4_HUMAN, Q9BYZ8 (See protein sequence)
Recommended Name: Regenerating islet-derived protein 4 precursor  
Size: 158 amino acids; 18230 Da
Subcellular location: Secreted
Sequence caution: Sequence=AAM95599.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing;
1 PDB 3D structure from and Proteopedia for REG4:
2KV3 (3D)    
Secondary accessions: Q8NER6 Q8NER7
Alternative splicing: 2 isoforms:  Q9BYZ8-1   Q9BYZ8-2   

Explore the universe of human proteins at neXtProt for REG4: NX_Q9BYZ8

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BYZ8

  • REG4 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001152824.1  NP_001152825.1  NP_114433.1  

    ENSEMBL proteins: 
     ENSP00000346158   ENSP00000256585   ENSP00000437135   ENSP00000358409  

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    Novus Biologicals REG4 Proteins
    Novus Biologicals REG4 Lysates
    Sino Biological Recombinant Protein for REG4
    ProSpec Recombinant Protein for REG4
    Uscn Proteins for REG4

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region IEA--


    REG4 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for REG4


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    REG4 for domains           About GeneDecksing

    4 InterPro domains/families:
     IPR016187 C-type_lectin_fold
     IPR018378 C-type_lectin_CS
     IPR016186 C-type_lectin-like
     IPR001304 C-type_lectin

    Graphical View of Domain Structure for InterPro Entry Q9BYZ8

    ProtoNet protein and cluster: Q9BYZ8

    1 Blocks protein family: IPB003990 Pancreatitis-associated protein signature

    UniProtKB/Swiss-Prot: REG4_HUMAN, Q9BYZ8
    Similarity: Contains 1 C-type lectin domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: REG4_HUMAN, Q9BYZ8
    Function: Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate
    recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the
    gastrointestinal epithelium
    Induction: Up-regulated by mucosal injury from active Crohn's disease or ulcerative colitis. Up-regulated in colorectal
    tumors. Up-regulated in epithelial cells at regenerating margins of peptic ulcers in the stomach and duodenum

    miRNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for REG4 (see all 7)
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for REG4

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0030246carbohydrate binding IEA--


    REG4 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Reg4tm1Lex for REG4
         1 MGI phenotypic allele for Reg4 (no phenotypes)

    REG4 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for REG4

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for REG4 (ENSP000002565854) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CUBNENSP000003670644STRING: ENSP00000367064
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    REG4 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for REG4
    1 Novoseek chemical compound relationship for REG4 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcium 4.37 3 15553756 (1), 16315915 (1), 19699332 (1)

    Search CenterWatch for drugs/clinical trials and news about REG4 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for REG4 gene (3 alternative transcripts): 
    NM_001159352.1  NM_001159353.1  NM_032044.3  

    Unigene Cluster for REG4:

    Regenerating islet-derived family, member 4
    Hs.660883  [show with all ESTs]
    Unigene Representative Sequence: AY126670
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000354219(uc001eig.3 uc001eif.3) ENST00000256585 ENST00000530654
    ENST00000369401(uc001eih.1)

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    Inhib. RNA
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    Additional cDNA sequence: 

    AF254415.1 AF345934.1 AY007243.1 AY126670.1 AY126671.1 AY126672.1 BC017089.2 

    6 DOTS entries:

    DT.417427  DT.92068854  DT.99927244  DT.100742664  DT.95167160  DT.95217366 

    24/199 AceView cDNA sequences (see all 199):

    BM769144 AA315757 BM796494 NM_032044 BM852931 AA315990 BC017089 AA316634 
    AY007243 AF254415 BM769915 AI791498 AW853800 AA314779 AA316658 BM852851 
    BM798142 BM769042 AA313200 BM773135 AA313549 AA307590 AI791286 AA315069 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for REG4    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b
    SP1:                                -                           
    SP2:              -                 -                           
    SP3:              -                                             


    ECgene alternative splicing isoforms for REG4

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    REG4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGAAGGTTTA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See REG4 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for REG4

    SOURCE GeneReport for Unigene cluster: Hs.660883

    UniProtKB/Swiss-Prot: REG4_HUMAN, Q9BYZ8
    Tissue specificity: Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum,
    appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly
    expressed in most colorectal tumors than in normal colon. Preferentially expressed in mucinous tumors and in some
    cases neuro-endocrine tumors. Expressed in mucus-secreting cells and enterocyte-like cells. In small intestine
    expressed at the basal perinuclear zone of goblet cells

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for REG4 gene from 4/11 species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves REG41 regenerating islet-derived family, member 4 52.93(n)
    40.54(a)
      424379  XM_422224.3  XP_422224.2 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    (see all 10)
    --
    42(a)
    37(a)
    (see all 10)
    1 ↔ many
    1 ↔ many
    (see all 10)
    GL343220.1(1307193-1311228)
    GL343220.1(1296818-1299765)
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkey-241l7.26
    si:dkey-241l7.56
    (see all 16)
    si:dkey-241l7.5
    (see all 16)
    33(a)
    33(a)
    (see all 16)
    many ↔ many
    many ↔ many
    (see all 16)
    20(54389663-54390997)
    20(54373156-54374435)
    worm
    (Caenorhabditis elegans)
    Secernentea clec-1486
    clec-506
    (see all 7)
    C-type LECtin family member (clec-50)
    (see all 7)
    19(a)
    14(a)
    (see all 7)
    many ↔ many
    many ↔ many
    (see all 7)
    III(174608-175832)
    V(19752371-19755333)


    ENSEMBL Gene Tree for REG4 (if available)
    TreeFam Gene Tree for REG4 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for REG4 gene
    REG3A2  REG3G2  CLEC19A2  REG1B2  REG1A2  
    12 SIMAP similar genes for REG4 using alignment to 2 protein entries:     REG4_HUMAN (see all proteins):
    REG1B    REG1A    PAP    REG3A    CD209    ASGR2
    CLEC4M    HBXBP    REG3G    CLEC10A    NCAN    CSPG3 variant protein

    REG4 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/413 NCBI SNPs in REG4 are shown (see all 413    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs66988131,2
    C,F,A,H,--120336182(+) CTTGTA/CTGGTG 2 -- ds500111Minor allele frequency- C:0.50NA WA CSA EA 374
    rs1932226431,2
    --120336244(+) AATCAC/TTGAGT 2 -- ds50010--------
    rs46592371,2
    C,F,A,H,--120336278(+) ACCACG/TCAGAT 2 -- ds500110Minor allele frequency- T:0.50NA WA CSA EA 371
    rs1856420211,2
    --120336367(+) TGCAAA/TCCTCA 2 -- ds50010--------
    rs1894375501,2
    --120336384(+) AAAGAC/GAGTCA 2 -- ds50010--------
    rs1167000791,2
    --120336561(+) CACCTG/TCATCT 2 -- ds50010--------
    rs1508216641,2
    --120336600(+) GGGGTA/GGAGGA 2 -- ds50010--------
    rs1807858811,2
    --120336735(+) TAAAGA/GAATGT 2 -- ut310--------
    rs10529721,2
    C,F,A,H,--120336785(-) GCCATG/AGGAAG 2 -- ut3131Minor allele frequency- A:0.43NS EA NA WA CSA EU 4815
    rs413061731,2
    --120336857(+) AGAAGC/GGTGTA 2 -- ut310--------

    HapMap Linkage Disequilibrium report for REG4 (120336641 - 120354283 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for REG4
         2 CNVs: 4245 3309

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    REG4 for disorders           About GeneDecksing

    OMIM gene information: 609846    OMIM disorders: --

    20/22 diseases for REG4 (see all 22):    About MalaCards
    pseudomyxoma peritonei    adenoid cystic carcinoma    peptic ulcer    pancreatic ductal adenocarcinoma
    gonorrhea    ulcerative colitis    adenoiditis    cystadenoma
    colon cancer    gastric cancer    crohn's disease    colon adenocarcinoma
    gastric adenocarcinoma    adenocarcinoma    pancreatitis    peritonitis
    pancreatic cancer    colorectal cancer    prostate cancer    adenoma

    7 Novoseek disease relationships for REG4 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    inflammatory bowel diseases 55.1 2 16086444 (1), 18754868 (1)
    colon cancer 52.2 8 17260007 (2), 19136934 (1), 19740514 (1)
    colitis ulcerative 32.2 3 17260007 (2), 12819006 (1)
    gastric cancer 25.3 13 15553756 (4), 16315915 (3)
    cancer 2.36 5 16086444 (1), 18754868 (1), 17260007 (1)
    metastasis 0 2 16315915 (1)
    tumors 0 2 18754868 (1), 12455032 (1)


    Export disorders for REG4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for REG4 gene, integrated from 9 sources (see all 46):
    (articles sorted by number of sources associating them with REG4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Reg IV, a new member of the regenerating gene family, is overexpressed in colorectal carcinomas. (PubMed id 12455032)1, 2, 3, 9 Violette S....Lesuffleur T. (2003)
    2. Isolation and characterization of a cDNA encoding a novel member of the human regenerating protein family: Reg IV. (PubMed id 11311942)1, 2, 3 Hartupee J.C.... Dieckgraefe B.K. (2001)
    3. RELP, a novel human REG-like protein with up-regulated expression in inflammatory and metaplastic gastrointestinal mucosa. (PubMed id 12819006)1, 2, 9 Kaemaeraeinen M....Andersson L.C. (2003)
    4. Human RegIV protein adopts a typical C-type lectin fo ld but binds mannan with two calcium-independent sites. (PubMed id 20692269)1, 2 Ho M.R....Chen C. (2010)
    5. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
    6. Signal peptide prediction based on analysis of experimentally verified cleavage sites. (PubMed id 15340161)1, 2 Zhang Z. and Henzel W.J. (2004)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    8. The role of Reg IV gene and its encoding product in g astric carcinogenesis. (PubMed id 19740514)1, 9 Zheng H.C....Takano Y. (2010)
    9. REG4 is associated with carcinogenesis in the 'intestinal' pathway of intraductal papillary mucinous neoplasms. (PubMed id 19136934)1, 9 Nakata K....Tsuneyoshi M. (2009)
    10. High expression of RELP (Reg IV) in neoplastic goblet cells of appendiceal mucinous cystadenoma and pseudomyxoma peritonei. (PubMed id 16323007)1, 9 Heiskala K....Andersson L.C. (2006)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 83998 HGNC: 22977 AceView: REG4 Ensembl:ENSG00000134193 euGenes: HUgn83998
    ECgene: REG4 H-InvDB: REG4

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    About This Section
    NameDescription
    PharmGKB entry for REG4 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for REG4 Genetics and Cytogenetics in Oncology and Haematology
    Functional Glycomics Gateway - Glycan Bindinghttp://www.functionalglycomics.org/glycomics/GBPServlet?&operationType=view&cbpId=cbp_hum_Ctlect_257

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    Patent Information for REG4 gene:
    Search GeneIP for patents involving REG4

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