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RCVRN Gene

protein-coding   GIFtS: 53

GC17M009742
recoverin
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: RCV1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
RCV1 2, 3
recoverin 2
Descriptions
Cancer-associated retinopathy protein 3
Protein CAR 3
cancer associated retinopathy antigen 2
External Ids
HGNC: 99371
Entrez Gene: 59572
UniProtKB: P352433
Ensembl: ENSG000001090477
Search outside databases for aliases for RCVRN gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for RCVRN:
This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein
contains three calcium-binding EF-hand domains and may prolong the termination of the
phototransduction cascade in the retina by blocking the phosphorylation of photo-activated
rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided
by RefSeq]

UniProtKB/Swiss-Prot: RECO_HUMAN, P35243
Function: Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin.
May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent
manner. The calcium-bound recoverin prolongs the photoresponse

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17p13.1   Ensembl cytogenetic band:  17p13.1   HGNC cytogenetic band: 17p13.1

RCVRN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M009742:     (about GC identifiers)

Start:
9,741,752 bp from pter
End:
9,749,409 bp from pter
Size:
7,658 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000017.9  NT_010718.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RECO_HUMAN, P35243 (See protein sequence)
Recommended Name: Recoverin  
Size: 200 amino acids; 23130 Da
Miscellaneous: Binds two calcium ions; one with high affinity, the other with low affinity
PDB structures from and Proteopedia :
2D8N (3D)    

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_002894.1  

    ENSEMBL proteins: 
    ENSP00000226193 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Recoverin)
    Human Recombinant Proteins from Abnova (RCVRN)
                  OriGene Purified Recombinant Human Protein: RCVRN 


    Antibodies for RCVRN: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of RCVRN
    Sigma-Aldrich Antibodies for RCVRN
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Recoverin), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (RCVRN)
    Novus Biologicals Antibody for RCV1

    Assays for RCVRN: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR018247 EF_HAND_1
     IPR018249 EF_HAND_2
     IPR001125 Recoverin
     IPR011992 EF-Hand_type
     IPR002048 EF_hand_Ca_bd


       GeneDecks  RCVRN for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P35243

    ProtoNet protein and cluster: P35243

    1 Blocks protein family: IPB001125 Recoverin family signature

    UniProtKB/Swiss-Prot: RECO_HUMAN, P35243
    Similarity: Belongs to the recoverin family
    Similarity: Contains 4 EF-hand domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RCVRN)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RCVRN)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002903

                  Applied Biosystems Silencer® siRNAs for RCVRN

                  Sigma-Aldrich siRNA for RCVRN  
                         Sigma-Aldrich shRNA for RCVRN  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for RCVRN
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002903
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002903
                                     untagged cDNA clone in CMV expression vector: NM_002903 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_002903

    UniProtKB/Swiss-Prot: RECO_HUMAN, P35243
    Function: Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin.
    May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent
    manner. The calcium-bound recoverin prolongs the photoresponse

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Rcvrn):

    vision/eye

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005509 calcium ion binding TAS1672047
    GO:0008048 calcium sensitive guanylate cyclase activator activity TAS1672047
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    1 Invitrogen iPath™ Online BioAtlas - Pathway for RCVRN (Maps provided by GeneGo):
     Rhodopsin phototransduction cascade in retina - the key role of Ca2+ transport

       GeneDecks  RCVRN for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  RCVRN  (Your Favorite Gene powered by Ingenuity) 
     Phototransduction Pathway

       GeneDecks  RCVRN for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  RCVRN 


    5/25 Interacting proteins for RCVRN (P352432 ENSP000002261933) via UniProtKB, MINT, and/or STRING (see all 25 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PQBP1O608282MINT-2862654
    GRK1ENSP000003348763STRING (score=.981)
    CRXENSP000002219963STRING (score=.897)
    PSEN2ENSP000003557463STRING (score=.89)
    PSEN1ENSP000003263663STRING (score=.881)
    About this table

    5 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165 signal transduction TAS1672047
    GO:0007601 visual perception TAS1672047
    GO:0007602 phototransduction IEA--
    GO:0050896 response to stimulus IEA--
    GO:0051924 regulation of calcium ion transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RCVRN
    8 Novoseek chemical compound relationships for RCVRN gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    guanylate 62.84 14 1672047 (3), 8961484 (1), 1422899 (1), 7678445 (1) (see all 11)
    calcium 60.08 210 11980481 (7), 7876221 (7), 9392525 (6), 9315839 (5) (see all 64)
    cgmp 47.08 7 1422899 (1), 7678445 (1), 8500558 (1), 18184973 (1) (see all 6)
    myristic acid 35.93 4 16376289 (3)
    phospholipid 13.18 5 9315839 (2), 12023256 (1), 12767213 (1), 9759500 (1)
    potassium 0.00 6 18458082 (1), 17962406 (1), 11535596 (1), 10483569 (1)
    lipid 0.00 10 18034895 (4), 11087403 (2), 12023256 (1), 8537345 (1) (see all 6)
    fatty acid 0.00 3 11980481 (1), 10625670 (1), 7797536 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RCVRN)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RCVRN)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002903

                  Sigma-Aldrich siRNA for RCVRN  
                         Sigma-Aldrich shRNA for RCVRN  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_002903  

    REFSEQ mRNAs for RCVRN gene: 

    NM_002903.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_002903  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002903
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002903
                                     untagged cDNA clone in CMV expression vector: NM_002903 

    Additional cDNA sequence: 

    AB001838.1 AK314129.1 BC001720.1 BT009838.1 DQ980605.1 DQ980606.1 DQ980608.1 DQ980609.1 
    S43855.1 S45545.1 S62028.1 

    4 DOTS entries:

    DT.317113  DT.120914699  DT.97759914  DT.95080229 

    24/139 AceView cDNA sequences (see all 139 ):

    BM662098 BM709331 BM709685 BU154132 H84778 BQ639095 BU726690 BQ639701 
    BQ640206 H87782 BQ637103 BU726857 BM662530 S43855 AA317683 BU732239 
    AA364912 H30195 BQ637550 AB001838 H30278 BQ639286 BQ638143 BQ638070 

    highest scoring ESTs for RCVRN:

    S43855 AA015656 AA317145 AA317683 AA364470 AA364705 AA365590 AA608727 AA773600 AB001838 

    Unigene Cluster for RCVRN:

    Recoverin
    Hs.80539  [show with all ESTs]
    Unigene Representative Sequence: NM_002903


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000226193  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RCVRN expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RCVRN

    1 / 2 / 3

    3 probe-sets matching RCVRN gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    37587_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 S43855 1.00 1.00 1.00 1

    206203_at2, 3 U133-A 1 1.00 1.00 -- -- NM_002903 0.60 1.00 0.82 1

    206203_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCCCTGTGCT

    SOURCE GeneReport for Unigene cluster: Hs.80539

    UniProtKB/Swiss-Prot: RECO_HUMAN, P35243
    Tissue specificity: Retina and pineal gland

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RCVRN gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RCVRN1   -- recoverin 88.72(n)
    88.89(a)
    489500  NM_001014281.1  NP_001014303.1 
    chimpanzee
    (Pan troglodytes)
    RCVRN1   -- recoverin 99.67(n)
    100(a)
    455078  XM_511841.2  XP_511841.2 
    cow
    (Bos taurus)
    RCVRN1   -- recoverin 89.06(n)
    88.89(a)
    281447  NM_174165.2  NP_776590.1 
    rat
    (Rattus norvegicus)
    Rcvrn1   -- recoverin 88.22(n)
    88.89(a)
    140936  NM_080901.1  NP_543177.1 
    mouse
    (Mus musculus)
    Rcvrn1, 5 11 (35.00 cM)5
    recoverin1, 5 87.54(n)1
    88.89(a)1
    196741  NM_009038.11  NP_033064.11 
     AL6460975  BC1105645  (see all 10)
    About this table        Species with no ortholog for RCVRN

    ENSEMBL Gene Tree for RCVRN
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RCVRN gene
    GUCA1B2  GUCA1A2  GUCA1C2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/98 NCBI SNPs in RCVRN are shown (see all 98 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 93)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs8872591,2
    A,C,F9742049(+) GCGCGC/TGTGTG 1 -- ut31 ese32Minor allele frequency- T:0.19MN EA 1656
    rs22865311,2
    C,F,H9749296(+) CAGGCT/GGGGCT 1 -- ut51 ese38Minor allele frequency- G:0.35EA EU WA NA 1996
    rs37605051,2
    C,F,H9749997(-) TCAAGA/GAAGAC 1 -- ng3110Minor allele frequency- G:0.30NA EA EU WA 814
    rs176820361,2
    C,F,H9741704(+) GACTGA/TTAGAA 1 -- ng5111Minor allele frequency- T:0.26NA EU EA WA 978
    rs37605061,2
    C,F,H9749944(-) CATCCG/AGGAAC 1 -- ng317Minor allele frequency- A:0.28NA EU EA WA 534
    rs118716301,2
    C,F,H9749875(+) atcatC/TGGCAC 1 -- ng314Minor allele frequency- T:0.01EU EA WA 420
    rs169589331,2
    F,H9742095(+) GTGTCA/GTGTGA 1 -- ut31 ese37Minor allele frequency- G:0.18NA EU EA WA 546
    rs37605041,2
    F,H9750161(-) CTGCTG/TGTCCC 1 -- ng314Minor allele frequency- T:0.04EU EA WA 410
    rs169589451,2
    F,H9749508(+) ATACTA/GGAACA 1 -- ng317Minor allele frequency- G:0.05NA EU EA WA 562
    rs72166231,2
    H9745124(+) GGGAGT/CGATCA 1 A/T mis1 ese34Minor allele frequency- C:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for RCVRN (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 179618

    UniProtKB/Swiss-Prot: RECO_HUMAN, P35243
  • Identified as the antigen in cancer-associated retinopathy, an autoimmune disease of the
    retina caused by a tumor in another tissue
  • 10/28 Novoseek disease relationships for RCVRN gene (see all 28 )

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    retinopathy 80.79 57 9727517 (3), 7568096 (3), 8892098 (2), 11180121 (2) (see all 39)
    paraneoplastic syndromes 80.00 10 11180121 (2), 16444517 (2), 12507501 (1), 15153841 (1) (see all 7)
    retinal degeneration 73.56 6 9727517 (2), 12507501 (1), 11074809 (1), 12935781 (1) (see all 5)
    retinitis pigmentosa 61.47 8 16934219 (4), 7906682 (2), 7607661 (1), 9071225 (1)
    small cell lung cancer 48.77 8 15301870 (2), 11557119 (1), 10740224 (1), 10766180 (1) (see all 7)
    visual loss 47.11 2 8912538 (1), 8794409 (1)
    blindness 32.25 2 9040460 (1)
    carcinoma non-small cell lung 31.12 2 15301870 (1), 15084384 (1)
    lung carcinoma 30.41 2 15065647 (1), 12838519 (1)
    uveitis 30.36 4 9727517 (2), 12424844 (1), 18421230 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/197 PubMed articles for RCVRN gene (see all 197 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5957 HGNC: 9937 AceView: RCV1 Ensembl:ENSG00000109047 euGenes: HUgn5957
    ECgene: RCVRN H-InvDB: RCVRN
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RCVRN:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for RCVRN Antibodies for RCVRN
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for RCVRN Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (Recoverin)
     Antibodies (Recoverin)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for RCVRN




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for RCV1

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