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Aliases for RBX1 Gene

Aliases for RBX1 Gene

  • Ring-Box 1 2 3 5
  • Regulator Of Cullins 1 2 3 4
  • Ring-Box 1, E3 Ubiquitin Protein Ligase 2 3
  • RING Finger Protein 75 3 4
  • RING-Box Protein 1 3 4
  • RNF75 3 4
  • ROC1 3 4
  • E3 Ubiquitin-Protein Ligase RBX1 3
  • ZYP Protein 3
  • Protein ZYP 4
  • BA554C12.1 3
  • EC 6.3.2.- 4
  • Rbx1 4

External Ids for RBX1 Gene

Previous GeneCards Identifiers for RBX1 Gene

  • GC22P037961
  • GC22P039590
  • GC22P039671
  • GC22P041347
  • GC22P024308

Summaries for RBX1 Gene

Entrez Gene Summary for RBX1 Gene

  • This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010]

GeneCards Summary for RBX1 Gene

RBX1 (Ring-Box 1) is a Protein Coding gene. Diseases associated with RBX1 include Invasive Bladder Transitional Cell Carcinoma and Renal Cell Carcinoma. Among its related pathways are Interleukin-3, 5 and GM-CSF signaling and Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template. GO annotations related to this gene include ligase activity and ubiquitin protein ligase binding. An important paralog of this gene is RNF7.

UniProtKB/Swiss-Prot for RBX1 Gene

  • E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Gene Wiki entry for RBX1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RBX1 Gene

Genomics for RBX1 Gene

Regulatory Elements for RBX1 Gene

Enhancers for RBX1 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
- Elite enhancer/Elite enhancer-gene association

Enhancers around RBX1 on UCSC Golden Path with GeneCards custom track

Promoters for RBX1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

Genomic Location for RBX1 Gene

Chromosome:
22
Start:
40,951,347 bp from pter
End:
40,973,309 bp from pter
Size:
21,963 bases
Orientation:
Plus strand

Genomic View for RBX1 Gene

Genes around RBX1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RBX1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RBX1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RBX1 Gene

Proteins for RBX1 Gene

  • Protein details for RBX1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P62877-RBX1_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RBX1
    Protein Accession:
    P62877
    Secondary Accessions:
    • B2RDY1
    • Q8N6Z8
    • Q9D1S2
    • Q9WUK9
    • Q9Y254

    Protein attributes for RBX1 Gene

    Size:
    108 amino acids
    Molecular mass:
    12274 Da
    Quaternary structure:
    • Part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of CBC(VHL) complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 or CUL5 and VHL. Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Part of multisubunit E3 ubiquitin ligase complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 and MED8; elongin BC complex (TCEB1 and TCEB2), CUL5 and MUF1. Part of multisubunit complexes with elongin BC complex (TCEB1 and TCEB2), elongin A/TCEB3 or SOCS1 or WSB1 and CUL5. Interacts directly with CUL1 and probably also with CUL2, CUL3, CUL4A, CUL4B, CUL5 and CUL7. Probably interacts with CDC34. Interacts with COPS6. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Part of an E3 ligase complex composed of RBX1, DDB1, DDB2 and CUL4A or CUL4B. Interacts with UBE2M. Part of a SCF complex consisting of CUL1, FBXO3, RBX1 and SKP1; this complex interacts with PML via FBXO3. Interacts with human adenovirus 5 E1A protein; this interaction inhibits RBX1-CUL1-dependent elongation reaction of ubiquitin chains by the SCF(FBW7) complex (PubMed:19679664). Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Identified in a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and CDC34. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein. Part of the BCR(ENC1) complex containing ENC1. Part of the BCR(GAN) complex containing GAN. Part of the BCR(KLHL41) complex containing KLHL41. Part of the BCR(KEAP1) complex containing KEAP1. Interacts with DCUN1D3. Interacts with SESN1 and SESN2 (PubMed:23274085).
    SequenceCaution:
    • Sequence=AAH17370.2; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RBX1 Gene

neXtProt entry for RBX1 Gene

Post-translational modifications for RBX1 Gene

  • Ubiquitination at Lys 19, Lys 20, and Lys 105
  • Modification sites at PhosphoSitePlus

Other Protein References for RBX1 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for RBX1 (RBX1)
  • Abcam antibodies for ROC1

No data available for DME Specific Peptides for RBX1 Gene

Domains & Families for RBX1 Gene

Gene Families for RBX1 Gene

Protein Domains for RBX1 Gene

Suggested Antigen Peptide Sequences for RBX1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P62877

UniProtKB/Swiss-Prot:

RBX1_HUMAN :
  • The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Belongs to the RING-box family.
  • Contains 1 RING-type zinc finger.
Domain:
  • The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
Family:
  • Belongs to the RING-box family.
Similarity:
  • Contains 1 RING-type zinc finger.
genes like me logo Genes that share domains with RBX1: view

Function for RBX1 Gene

Molecular function for RBX1 Gene

GENATLAS Biochemistry:
ring-box 1
UniProtKB/Swiss-Prot Function:
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Enzyme Numbers (IUBMB) for RBX1 Gene

Gene Ontology (GO) - Molecular Function for RBX1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity TAS --
GO:0005515 protein binding IEA,IPI 11961546
GO:0008270 zinc ion binding IEA --
GO:0016874 ligase activity IEA --
GO:0019788 NEDD8 transferase activity IDA 19250909
genes like me logo Genes that share ontologies with RBX1: view
genes like me logo Genes that share phenotypes with RBX1: view

Animal Model Products

  • Taconic Biosciences Mouse Models for RBX1

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for RBX1

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RBX1 Gene

Localization for RBX1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RBX1 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RBX1 Gene COMPARTMENTS Subcellular localization image for RBX1 gene
Compartment Confidence
cytosol 5
nucleus 5
extracellular 3

Gene Ontology (GO) - Cellular Components for RBX1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
GO:0005829 cytosol IEA,TAS --
GO:0019005 SCF ubiquitin ligase complex IEA,ISS --
GO:0030891 VCB complex IEA --
GO:0031461 cullin-RING ubiquitin ligase complex IDA 22405651
genes like me logo Genes that share ontologies with RBX1: view

Pathways & Interactions for RBX1 Gene

SuperPathways for RBX1 Gene

Superpath Contained pathways
1 Interleukin-3, 5 and GM-CSF signaling
2 Signaling by NOTCH1
3 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
4 CDK-mediated phosphorylation and removal of Cdc6
5 Infectious disease
.48
genes like me logo Genes that share pathways with RBX1: view

UniProtKB/Swiss-Prot P62877-RBX1_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

SIGNOR curated interactions for RBX1 Gene

Activates:

Gene Ontology (GO) - Biological Process for RBX1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000209 protein polyubiquitination TAS --
GO:0000715 nucleotide-excision repair, DNA damage recognition TAS --
GO:0000717 nucleotide-excision repair, DNA duplex unwinding TAS --
GO:0006283 transcription-coupled nucleotide-excision repair TAS --
genes like me logo Genes that share ontologies with RBX1: view

Drugs & Compounds for RBX1 Gene

(2) Drugs for RBX1 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(1) Additional Compounds for RBX1 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with RBX1: view

Transcripts for RBX1 Gene

mRNA/cDNA for RBX1 Gene

Unigene Clusters for RBX1 Gene

Ring-box 1, E3 ubiquitin protein ligase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for RBX1

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RBX1 Gene

No ASD Table

Relevant External Links for RBX1 Gene

GeneLoc Exon Structure for
RBX1
ECgene alternative splicing isoforms for
RBX1

Expression for RBX1 Gene

mRNA expression in normal human tissues for RBX1 Gene

Protein differential expression in normal tissues from HIPED for RBX1 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (6.7) and Frontal cortex (6.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for RBX1 Gene



Protein tissue co-expression partners for RBX1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RBX1 Gene:

RBX1

SOURCE GeneReport for Unigene cluster for RBX1 Gene:

Hs.474949

mRNA Expression by UniProt/SwissProt for RBX1 Gene:

P62877-RBX1_HUMAN
Tissue specificity: Widely expressed.
genes like me logo Genes that share expression patterns with RBX1: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for RBX1 Gene

Orthologs for RBX1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RBX1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RBX1 34
  • 97.22 (n)
  • 100 (a)
RBX1 35
  • 98 (a)
OneToMany
RBX1 35
  • 100 (a)
OneToMany
dog
(Canis familiaris)
Mammalia RBX1 34
  • 97.84 (n)
  • 100 (a)
mouse
(Mus musculus)
Mammalia Gm9840 34
  • 91.98 (n)
  • 98.15 (a)
Gm9840 16
Rbx1 35
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RBX1 34
  • 99.69 (n)
  • 100 (a)
RBX1 35
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RBX1 35
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RBX1 35
  • 100 (a)
OneToOne
chicken
(Gallus gallus)
Aves RBX1 34
  • 87.35 (n)
  • 100 (a)
RBX1 35
  • 100 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RBX1 35
  • 99 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rbx1 34
  • 81.17 (n)
  • 97.22 (a)
zebrafish
(Danio rerio)
Actinopterygii rbx1 34
  • 82.72 (n)
  • 98.15 (a)
rbx1 35
  • 98 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Roc1a 36
  • 71 (a)
Roc1b 36
  • 70 (a)
Roc1a 34
  • 78.67 (n)
  • 92 (a)
Roc1a 35
  • 68 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005202 34
  • 75.64 (n)
  • 90.38 (a)
worm
(Caenorhabditis elegans)
Secernentea rbx-1 34
  • 72.9 (n)
  • 82.24 (a)
rbx-1 35
  • 80 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes HRT1 35
  • 52 (a)
OneToMany
HRT1 37
thale cress
(Arabidopsis thaliana)
eudicotyledons RBX1 34
  • 71.67 (n)
  • 87 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.459 34
barley
(Hordeum vulgare)
Liliopsida Hv.2894 34
rice
(Oryza sativa)
Liliopsida Os.20521 34
wheat
(Triticum aestivum)
Liliopsida Ta.5537 34
corn
(Zea mays)
Liliopsida Zm.4011 34
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.4811 34
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rbx1 34
  • 67.6 (n)
  • 74.77 (a)
Species where no ortholog for RBX1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for RBX1 Gene

ENSEMBL:
Gene Tree for RBX1 (if available)
TreeFam:
Gene Tree for RBX1 (if available)

Paralogs for RBX1 Gene

Paralogs for RBX1 Gene

(2) SIMAP similar genes for RBX1 Gene using alignment to 1 proteins:

Pseudogenes.org Pseudogenes for RBX1 Gene

genes like me logo Genes that share paralogs with RBX1: view

Variants for RBX1 Gene

Sequence variations from dbSNP and Humsavar for RBX1 Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type
rs5995968 -- 40,949,802(+) aggct(A/G)gtctc upstream-variant-2KB
rs5995969 -- 40,954,145(+) atgtc(G/T)gtaat intron-variant
rs5995970 -- 40,956,220(+) TACCT(A/G)TTTTG intron-variant
rs5995971 -- 40,958,423(+) ATTTA(A/G)CAACG intron-variant
rs5995972 -- 40,962,889(+) gacgg(G/T)gtttc intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RBX1 Gene

Variant ID Type Subtype PubMed ID
nsv1058653 CNV gain 25217958
nsv428389 CNV loss 18775914

Variation tolerance for RBX1 Gene

Residual Variation Intolerance Score: 54.8% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RBX1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RBX1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RBX1 Gene

Disorders for RBX1 Gene

MalaCards: The human disease database

(5) MalaCards diseases for RBX1 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
invasive bladder transitional cell carcinoma
  • invasive bladder urothelial carcinoma
renal cell carcinoma
  • renal cell carcinoma, clear cell, somatic
renal clear cell carcinoma
  • clear cell carcinoma of kidney
familial hypertension
  • genetic hypertension
retinitis pigmentosa
  • retinitis pigmentosa 1
- elite association - COSMIC cancer census association via MalaCards
Search RBX1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RBX1

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RBX1
genes like me logo Genes that share disorders with RBX1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RBX1 Gene

Publications for RBX1 Gene

  1. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. (PMID: 10230407) Ohta T. … Xiong Y. (Mol. Cell 1999) 2 3 4 22 65
  2. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. (PMID: 19250909) Huang D.T. … Schulman B.A. (Mol. Cell 2009) 3 4 22 65
  3. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. (PMID: 12732143) Groisman R. … Nakatani Y. (Cell 2003) 3 4 22 65
  4. CUL7: a DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. (PMID: 12481031) Dias D.C. … Pan Z.Q. (Proc. Natl. Acad. Sci. U.S.A. 2002) 3 4 22 65
  5. The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. (PMID: 11027288) Furukawa M. … Xiong Y. (Mol. Cell. Biol. 2000) 3 4 22 65

Products for RBX1 Gene

Sources for RBX1 Gene

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