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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RBX1 Gene

protein-coding   GIFtS: 63
GCID: GC22P041347

Ring-Box 1, E3 Ubiquitin Protein Ligase

(Previous name: ring-box 1)
Microbiology & Infectious Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Ring-Box 1, E3 Ubiquitin Protein Ligase1 2     BA554C12.12
Regulator Of Cullins 11 2 3     E3 Ubiquitin-Protein Ligase RBX12
RNF752 3     RING Box Protein 12
ROC12 3     ZYP Protein2
RING Finger Protein 752 3     EC 6.3.2.-3
RING-Box Protein 12 3     Rbx13
Ring-Box 11     Protein ZYP3

External Ids:    HGNC: 99281   Entrez Gene: 99782   Ensembl: ENSG000001003877   OMIM: 6038145   UniProtKB: P628773   

Export aliases for RBX1 gene to outside databases

Previous GC identifers: GC22P037961 GC22P039590 GC22P039671 GC22P024308


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RBX1 Gene:
This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin
proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein
may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.(provided by RefSeq, Sep 2010)

GeneCards Summary for RBX1 Gene: 
RBX1 (ring-box 1, E3 ubiquitin protein ligase) is a protein-coding gene. Diseases associated with RBX1 include 3-m syndrome, and glomuvenous malformation, and among its related super-pathways are Antigen processing: Ubiquitination & Proteasome degradation and Constitutive Signaling by NOTCH1 PEST Domain Mutants. GO annotations related to this gene include ubiquitin-protein ligase activity and protein complex binding. An important paralog of this gene is RNF7.

UniProtKB/Swiss-Prot: RBX1_HUMAN, P62877
Function: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which
mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved
in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision
repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate
recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in
proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like
CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34
autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and
for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
Involved in the ubiquitination of KEAP1, ENC1 and KLHL41

Gene Wiki entry for RBX1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000022.10  NC_018933.2  NT_011520.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RBX1 gene promoter:
         HOXA9   AhR   Pax-3   AML1a   p53   Arnt   C/EBPalpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRBX1 promoter sequence
   Search SABiosciences Chromatin IP Primers for RBX1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RBX1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 22q13.2   Ensembl cytogenetic band:  22q13.2   HGNC cytogenetic band: 22q13.2

RBX1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RBX1 gene location

GeneLoc information about chromosome 22         GeneLoc Exon Structure

GeneLoc location for GC22P041347:  view genomic region     (about GC identifiers)

Start:
41,347,351 bp from pter      End:
41,369,313 bp from pter
Size:
21,963 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: RBX1_HUMAN, P62877 (See protein sequence)
Recommended Name: E3 ubiquitin-protein ligase RBX1  
Size: 108 amino acids; 12274 Da
Subunit: Part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Part of a SCF-like complex consisting of
CUL7, RBX1, SKP1 and FBXW8. Part of CBC(VHL) complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 or CUL5
and VHL. Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing
ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts
with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Part of
multisubunit E3 ubiquitin ligase complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 and MED8; elongin BC
complex (TCEB1 and TCEB2), CUL5 and MUF1. Part of multisubunit complexes with elongin BC complex (TCEB1 and
TCEB2), elongin A/TCEB3 or SOCS1 or WSB1 and CUL5. Interacts directly with CUL1 and probably also with CUL2,
CUL3, CUL4A, CUL4B, CUL5 and CUL7. Probably interacts with CDC34. Interacts with COPS6. Component of the DCX
DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Part of an E3 ligase
complex composed of RBX1, DDB1, DDB2 and CUL4A or CUL4B. Interacts with UBE2M. Part of a SCF complex consisting
of CUL1, FBXO3, RBX1 and SKP1; this complex interacts with PML via FBXO3. Interacts with human adenovirus early
E1A protein; this interaction inhibits RBX1-CUL1-dependent elongation reaction of ubiquitin chains by the
SCF(FBW7) complex. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Identified in
a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and CDC34. Component of multiple
BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB
domain-containing protein. Part of the BCR(ENC1) complex containing ENC1. Part of the BCR(GAN) complex containing
GAN. Part of the BCR(KLHL41) complex containing KLHL41. Part of the BCR(KEAP1) complex containing KEAP1
Subcellular location: Cytoplasm. Nucleus
Sequence caution: Sequence=AAH17370.2; Type=Erroneous initiation;
6/9 PDB 3D structures from and Proteopedia for RBX1 (see all 9):
1LDJ (3D)        1LDK (3D)        1U6G (3D)        2HYE (3D)        2LGV (3D)        3DPL (3D)    
Secondary accessions: B2RDY1 Q8N6Z8 Q9D1S2 Q9WUK9 Q9Y254

Explore the universe of human proteins at neXtProt for RBX1: NX_P62877

Explore proteomics data for RBX1 at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_P62877

  • RBX1 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    RBX1 Protein Expression
    REFSEQ proteins: NP_055063.1  
    ENSEMBL proteins: 
     ENSP00000216225  
    Reactome Protein details: P62877
    Human Recombinant Protein Products for RBX1: 
    Browse Purified and Recombinant Proteins at EMD Millipore
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    Enzo Life Sciences proteins for RBX1
    OriGene Purified Protein for RBX1
    OriGene Protein Over-expression Lysate for RBX1
    OriGene MassSpec for RBX1 
    OriGene Custom Protein Services for RBX1
    GenScript Custom Purified and Recombinant Proteins Services for RBX1
    Novus Biologicals RBX1 Protein
    Novus Biologicals RBX1 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for RBX1 

    Gene Ontology (GO): 5/10 cellular component terms (GO ID links to tree view) (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005654nucleoplasm TAS--
    GO:0005829cytosol TAS--
    GO:0019005SCF ubiquitin ligase complex ISS--
    GO:0030891VCB complex IEA--
    GO:0031461cullin-RING ubiquitin ligase complex IDA--

    RBX1 for ontologies           About GeneDecksing



    RBX1 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of RBX1
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    Cell Signaling Technology (CST) Antibodies for RBX1 
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    Novus Biologicals RBX1 Antibodies
    Abcam antibodies for RBX1
    Cloud-Clone Corp. Antibodies for RBX1 
    ThermoFisher Antibody for RBX1
    LSBio Antibodies in human, mouse, rat for RBX1 

    Assay Products for RBX1: 
    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for RBX1
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for RBX1 
    Cloud-Clone Corp. CLIAs for RBX1


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    RNF: RING-type (C3HC4) zinc fingers

    3 InterPro protein domains:
     IPR001841 Znf_RING
     IPR013083 Znf_RING/FYVE/PHD
     IPR024766 Znf_RING_H2

    Graphical View of Domain Structure for InterPro Entry P62877

    ProtoNet protein and cluster: P62877

    1 Blocks protein domain: IPB001841 Zn-finger

    UniProtKB/Swiss-Prot: RBX1_HUMAN, P62877
    Domain: The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional
    third zinc ion
    Similarity: Belongs to the RING-box family
    Similarity: Contains 1 RING-type zinc finger


    RBX1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RBX1_HUMAN, P62877
    Function: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which
    mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved
    in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision
    repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate
    recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in
    proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like
    CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34
    autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and
    for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.
    Involved in the ubiquitination of KEAP1, ENC1 and KLHL41

         Genatlas biochemistry entry for RBX1:
    ring-box 1

         Enzyme Number (IUBMB): EC 6.3.2.-1

         Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842contributes to ubiquitin-protein ligase activity IDA15983046
    GO:0005515protein binding IPI11961546
    GO:0008270zinc ion binding IEA--
    GO:0019788NEDD8 ligase activity IDA19250909
    GO:0031625ubiquitin protein ligase binding IDA17636018
         
    RBX1 for ontologies           About GeneDecksing


    Phenotypes:
         10 GenomeRNAi human phenotypes for RBX1:
     Decreased Salmonella enterica   Decreased Salmonella enterica   Decreased Salmonella enterica   Decreased Salmonella enterica  
     Decreased Salmonella enterica   Decreased Salmonella-containin  Decreased viability of wild-ty  Increased cell death in HCC-19 
     Increased cell death in breast  Increased circadian period len 

         4 MGI mutant phenotypes (inferred from 1 allele(MGI details for Rbx1):
     cellular  embryogenesis  growth/size  mortality/aging 

    RBX1 for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory - Custom generated mouse model solutions for RBX1 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for RBX1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for RBX1 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for RBX1 

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RBX1
    8/12 QIAGEN miScript miRNA Assays for microRNAs that regulate RBX1 (see all 12):
    hsa-miR-100* hsa-miR-495 hsa-miR-7-2* hsa-miR-135b hsa-miR-338-5p hsa-miR-7-1* hsa-miR-514 hsa-miR-194
    SwitchGear 3'UTR luciferase reporter plasmidRBX1 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for RBX1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RBX1

    Gene Editing
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    Sirion Biotech Customized adenovirus for overexpression of RBX1

    Clone
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    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for RBX1 (see all 7)
    OriGene ORF clones in mouse, rat for RBX1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: RBX1 (NM_014248)
    Sino Biological Human cDNA Clone for RBX1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RBX1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RBX1
    Sirion Biotech Customized lentivirus for stable overexpression of RBX1 
                         Customized lentivirus expression plasmids for stable overexpression of RBX1 

    Cell Line
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RBX1


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for RBX1 About   (see all 33)                                                                                              See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Antigen processing: Ubiquitination & Proteasome degradation
    Antigen processing: Ubiquitination & Proteasome degradation0.83
    Ubiquitin mediated proteolysis0.36
    Class I MHC mediated antigen processing & presentation0.83
    2Signaling by NOTCH1 t(7 9)(NOTCH1:M1580 K2555) Translocation Mutant
    Constitutive Signaling by NOTCH1 PEST Domain Mutants0.89
    Signaling by NOTCH1 in Cancer0.79
    Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants0.89
    Signaling by NOTCH10.79
    Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer0.79
    Signaling by NOTCH1 t(7 9)(NOTCH1:M1580 K2555) Translocation Mutant0.79
    Signaling by NOTCH1 PEST Domain Mutants in Cancer0.79
    Signaling by NOTCH0.74
    3Cellular response to hypoxia
    Cellular response to hypoxia0.72
    Regulation of Hypoxia-inducible Factor (HIF) by Oxygen0.72
    Cellular responses to stress0.72
    Oxygen-dependent Proline Hydroxylation of Hypoxia-inducible Factor Alpha0.72
    4Cell cycle Cell cycle (generic schema)
    Cell cycle Role of SCF complex in cell cycle regulation0.39
    Cell cycle ESR1 regulation of G1/S transition0.33
    Cell cycle Regulation of G1/S transition (part 1)0.39
    Immune response MIF-JAB1 signaling0.32
    5HIV Life Cycle
    HIV Infection0.61
    Host Interactions of HIV factors0.60

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    5/6 EMD Millipore Pathways for RBX1 (see all 6)
        Common schema of ubiquitination
    Cell cycle Regulation of G1/S transition (part 1)
    Cell cycle ESR1 regulation of G1/S transition
    Proteolysis Putative ubiquitin pathway
    Immune response MIF-JAB1 signaling

    3 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for RBX1
        HIF1Alpha Pathway
    JAK-STAT Pathway
    SOCS Pathway

    1 Cell Signaling Technology (CST) Pathway for RBX1
        Protein Stability

    5 GeneGo (Thomson Reuters) Pathways for RBX1
        Cell cycle ESR1 regulation of G1/S transition
    Cell cycle Regulation of G1/S transition (part 1)
    Proteolysis Putative ubiquitin pathway
    Cell cycle Role of SCF complex in cell cycle regulation
    Immune response MIF-JAB1 signaling

    3 BioSystems Pathways for RBX1
        TGF-beta Receptor Signaling Pathway
    Delta-Notch Signaling Pathway
    Hypoxic and oxygen homeostasis regulation of HIF-1-alpha


    5/31        Reactome Pathways for RBX1 (see all 31)
        Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants
    Loss of Function of FBXW7 in Cancer and NOTCH1 Signaling
    NOTCH1 Intracellular Domain Regulates Transcription
    FBXW7 Mutants and NOTCH1 in Cancer
    Prolactin receptor signaling


    5/11         Kegg Pathways  (Kegg details for RBX1) (see all 11):
        Nucleotide excision repair
    HIF-1 signaling pathway
    Cell cycle
    Oocyte meiosis
    Ubiquitin mediated proteolysis

    UniProtKB/Swiss-Prot: RBX1_HUMAN, P62877
    Pathway: Protein modification; protein ubiquitination


    RBX1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for RBX1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/369 Interacting proteins for RBX1 (P628771, 2, 3 ENSP000002162254) via UniProtKB, MINT, STRING, and/or I2D (see all 369)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    HSPA1AP081073, ENSP000003648024I2D: score=1 STRING: ENSP00000364802
    ENSG00000212866P081073I2D: score=1 
    ENSG00000215328P081073I2D: score=1 
    ENSG00000224501P081073I2D: score=1 
    ENSG00000231555P081073I2D: score=1 
    About this table

    Gene Ontology (GO): 5/12 biological process terms (GO ID links to tree view) (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006281DNA repair IEA--
    GO:0006513protein monoubiquitination IDA--
    GO:0007219Notch signaling pathway TAS--
    GO:0016032viral process TAS--
    GO:0016567protein ubiquitination IDA15103331

    RBX1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    RBX1 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RBX1

    3 Novoseek inferred chemical compound relationships for RBX1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    zinc 42.6 1 18368307 (1)
    steroid 0 3 9710597 (1), 16874068 (1), 9205114 (1)
    leucine 0 1 12615916 (1)

    Search CenterWatch for drugs/clinical trials and news about RBX1

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    About This Section

    REFSEQ mRNAs for RBX1 gene: 
    NM_014248.3  

    Unigene Cluster for RBX1:

    Ring-box 1, E3 ubiquitin protein ligase
    Hs.474949  [show with all ESTs]
    Unigene Representative Sequence: BU155800
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000476110 ENST00000216225(uc003azk.3) ENST00000467617
    Congresses - knowledge worth sharing:  
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    8/12 QIAGEN miScript miRNA Assays for microRNAs that regulate RBX1 (see all 12):
    hsa-miR-100* hsa-miR-495 hsa-miR-7-2* hsa-miR-135b hsa-miR-338-5p hsa-miR-7-1* hsa-miR-514 hsa-miR-194
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    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RBX1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RBX1
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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RBX1

    Additional mRNA sequence: 

    AF085906.1 AF140598.1 AF142059.1 AK315722.1 AY099360.1 BC001466.2 BC017370.1 CR456560.1 

    12 DOTS entries:

    DT.99988559  DT.91760128  DT.100825475  DT.91751456  DT.120645894  DT.95124659  DT.95124675  DT.75152194 
    DT.97809928  DT.100037803  DT.120645905  DT.95300188 

    24/305 AceView cDNA sequences (see all 305):

    BU169268 BE256643 AI343871 BQ670844 BM697883 BM715215 CB113476 W81385 
    BQ880298 BG054985 BG478622 AW239094 AF085906 BM452435 CR456560 AW117478 
    BM667060 F25684 AI494283 BM670693 BC001466 AA658037 AW163628 AI310807 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RBX1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTTTTCACTT
    RBX1 Expression
    About this image


    See RBX1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RBX1

    SOURCE GeneReport for Unigene cluster: Hs.474949

    UniProtKB/Swiss-Prot: RBX1_HUMAN, P62877
    Tissue specificity: Widely expressed

        SABiosciences Expression via Pathway-Focused PCR Array including RBX1: 
              Ubiquitin Ligases in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for RBX1 gene from 10/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rbx15
    Gm98401
    ring-box 15
    predicted gene 98401
    91.98(n)1
    98.15(a)1
      15 (38.11 cM)5
    1000436741  XM_001480943.31  XP_001480993.21 
     814663165 
    chicken
    (Gallus gallus)
    Aves RBX16
    RING-box protein 1
    100(a)
    1 ↔ 1
    1(49718304-49729337)
    lizard
    (Anolis carolinensis)
    Reptilia RBX16
    Uncharacterized protein
    99(a)
    1 ↔ 1
    5(26051993-26057350)
    African clawed frog
    (Xenopus laevis)
    Amphibia BJ621900.12   -- 81.6(n)    BJ621900.1 
    zebrafish
    (Danio rerio)
    Actinopterygii AI106413.12   -- 82.74(n)    AI106413.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Roc1a3 smoothened receptor signaling pathway 71(a)
    (best of 2)
      1B13   --
    worm
    (Caenorhabditis elegans)
    Secernentea rbx-16
    RING-box protein 1
    77(a)
    1 ↔ 1
    V(9689682-9690615)
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes HRT1(YOL133W)4 RING finger containing subunit of Skp1-Cullin-F-box more   --   15(70325-70690) 853986  NP_014508.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons RBX11 RING-box protein 1 72.11(n)
    87.76(a)
      832179  NM_001161254.1  NP_001154726.1 
    rice
    (Oryza sativa)
    Liliopsida Os.205212 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 80.92(n)    AK099287.1 


    ENSEMBL Gene Tree for RBX1 (if available)
    TreeFam Gene Tree for RBX1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RBX1 gene
    RNF72  
    2 SIMAP similar genes for RBX1 using alignment to 1 protein entry:     RBX1_HUMAN:
    RNF7    ANAPC11

    RBX1 for paralogs           About GeneDecksing


    2 Pseudogenes.org Pseudogenes for RBX1
    PGOHUM00000240695 PGOHUM00000235674


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/392 SNPs in RBX1 are shown (see all 392)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 22 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1894705221,2
    --41345587(+) CTTTCC/TCTTTT 1 -- us2k10--------
    rs1494170361,2
    C--41345603(+) TTTTTC/TTGGGG 1 -- us2k10--------
    rs1815815011,2
    --41345604(+) TTTTTG/TGGGGG 1 -- us2k10--------
    rs1871238261,2
    --41345776(+) TAGTAA/GGGACG 1 -- us2k10--------
    rs59959681,2
    C,F,A--41345806(+) aggctG/Agtctc 1 -- us2k11Minor allele frequency- A:0.50NA 2
    rs1430806861,2
    --41345814(+) CTCAAA/TCTCCT 1 -- us2k10--------
    rs1460494591,2
    --41345988(+) TCTAAC/GAGTGC 1 -- us2k10--------
    rs584575761,2
    C--41346225(+) AAAAG-/AAAAAA 1 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs1400283611,2
    C--41346252(+) GCACTG/TTGGGA 1 -- us2k10--------
    rs1894937131,2
    --41346543(+) GGTTTA/CACTAC 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for RBX1 (41347351 - 41369313 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for RBX1:    About this table     
    Variant IDTypeSubtypePubMed ID
    nsv428389CNV Loss18775914

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 603814    OMIM disorders: --

    20/28 diseases for RBX1 (see all 28):    About MalaCards
    3-m syndrome    glomuvenous malformation    mulibrey nanism    skin melanoma
    von hippel-lindau disease    smith magenis syndrome    kidney cancer    renal clear cell carcinoma
    xeroderma pigmentosum    gout    amyloidosis    retinitis pigmentosa
    hypoxia    diffuse large b-cell lymphoma    mental retardation    renal cell carcinoma
    thyroid cancer    b-cell lymphomas    multiple myeloma    melanoma


    RBX1 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:  
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    8 Novoseek inferred disease relationships for RBX1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    smith-magenis syndrome 68.5 1 9615224 (1)
    carcinoma renal cell 30.2 1 11526493 (1)
    promyelocytic leukemia 27.9 1 12837286 (1)
    tumors 6.74 5 19509229 (2), 9452416 (1), 10648623 (1)
    necrosis 0 2 10648623 (1), 7639698 (1)
    breast cancer 0 1 19536326 (1)
    mental retardation 0 1 9615224 (1)
    cancer 0 5 19509229 (3), 17296600 (1), 19818716 (1)


    Export disorders for RBX1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RBX1 gene, integrated from 9 sources (see all 329):
    (articles sorted by number of sources associating them with RBX1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. (PubMed id 10230407)1, 2, 3, 9 Ohta T.... Xiong Y. (1999)
    2. Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. (PubMed id 10213691)1, 2, 3 Kamura T.... Conaway J.W. (1999)
    3. The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. (PubMed id 11027288)1, 2, 9 Furukawa M.... Xiong Y. (2000)
    4. Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase. (PubMed id 14739464)1, 2, 9 Wertz I.E.... Dixit V.M. (2004)
    5. CUL7: a DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. (PubMed id 12481031)1, 2, 9 Dias D.C.... Pan Z.Q. (2002)
    6. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. (PubMed id 19250909)1, 2, 9 Huang D.T....Schulman B.A. (2009)
    7. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. (PubMed id 12732143)1, 2, 9 Groisman R.... Nakatani Y. (2003)
    8. SCF(Cyclin F) controls centrosome homeostasis and mit otic fidelity through CP110 degradation. (PubMed id 20596027)1, 2 D'Angiolella V....Pagano M. (2010)
    9. Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src. (PubMed id 19112177)1, 2 Cen B....Weinstein I.B. (2009)
    10. PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation. (PubMed id 18809579)1, 2 Shima Y....Kitabayashi I. (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 9978 HGNC: 9928 AceView: RBX1 Ensembl:ENSG00000100387 euGenes: HUgn9978
    ECgene: RBX1 Kegg: 9978 H-InvDB: RBX1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RBX1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for RBX1 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RBX1 gene:
    Search GeneIP for patents involving RBX1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    About This Section

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    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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