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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RBM28 Gene

protein-coding   GIFtS: 53
GCID: GC07M127951

RNA binding motif protein 28

 Explore 12 diseases affiliated with
RBM28 via our new
 Human Malady Compendium 
Biological research products
for RBM28
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
RNA Binding Motif Protein 281 2
FLJ103771
2810480G15Rik2
RNA-Binding Protein 282
RNA-Binding Motif Protein 283

External Ids:    HGNC: 218631   Entrez Gene: 551312   Ensembl: ENSG000001063447   OMIM: 6120745   UniProtKB: Q9NW133   

Export aliases for RBM28 gene to outside databases

Previous GC identifers: GC07M127505 GC07M127545 GC07M127737 GC07M122311


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RBM28:
The protein encoded by this gene is a specific nucleolar component of the spliceosomal small nuclear ribonucleoprotein
(snRNP)complexes . It specifically associates with U1, U2, U4, U5, and U6 small nuclear RNAs (snRNAs), possibly
coordinating their transition through the nucleolus. Mutation in this gene causes alopecia, progressive neurological
defects, and endocrinopathy (ANE syndrome), a pleiotropic and clinically heterogeneous disorder. Alternatively spliced
transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Oct 2009)

UniProtKB/Swiss-Prot: RBM28_HUMAN, Q9NW13
Function: Nucleolar component of the spliceosomal ribonucleoprotein complexes

Gene Wiki entry for RBM28


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000007.13  NC_018918.1  NT_007933.15  NT_079596.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RBM28 gene promoter:
         Egr-2   POU2F1   POU2F1a   HFH-1   Pax-2   Sox9   IRF-7A   Pax-2a   E47   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRBM28 promoter sequence
   Search SABiosciences Chromatin IP Primers for RBM28

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RBM28


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q32.1   Ensembl cytogenetic band:  7q32.1   HGNC cytogenetic band: 7q32.2

RBM28 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RBM28 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M127951:  view genomic region     (about GC identifiers)

Start:
127,950,436 bp from pter      End:
127,983,962 bp from pter
Size:
33,527 bases      Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 127,333,546-127,367,073     

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RBM28_HUMAN, Q9NW13 (See protein sequence)
Recommended Name: RNA-binding protein 28  
Size: 759 amino acids; 85738 Da
Subunit: Interacts with U1, U2, U4, U5, and U6 spliceosomal small nuclear RNAs (snRNAs)
Subcellular location: Nucleus, nucleolus
Secondary accessions: A4D100 B4DU52 E9PDD9 Q53H65 Q96CV3
Alternative splicing: 2 isoforms:  Q9NW13-1   Q9NW13-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for RBM28: NX_Q9NW13

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9NW13

  • RBM28 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001159607.1  NP_060547.2  

    ENSEMBL proteins: 
     ENSP00000223073   ENSP00000390517   ENSP00000419840   ENSP00000418071   ENSP00000420503  

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    Uscn Proteins for RBM28

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005681spliceosomal complex IEA--
    GO:0005730nucleolus IDA--
    GO:0005794Golgi apparatus IDA--


    RBM28 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RBM28 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR012677 Nucleotide-bd_a/b_plait
     IPR000504 RRM_dom

    Graphical View of Domain Structure for InterPro Entry Q9NW13

    ProtoNet protein and cluster: Q9NW13

    1 Blocks protein family: IPB000504 RNA-binding region RNP-1 (RNA recognition motif)

    UniProtKB/Swiss-Prot: RBM28_HUMAN, Q9NW13
    Similarity: Contains 4 RRM (RNA recognition motif) domains


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RBM28_HUMAN, Q9NW13
    Function: Nucleolar component of the spliceosomal ribonucleoprotein complexes

    miRNA
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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding IEA--
    GO:0003676nucleic acid binding ----
    GO:0003723RNA binding IEA--


    RBM28 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Ribosome biogenesis in eukaryotes
    Ribosome biogenesis in eukaryotes1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways



    1         Kegg Pathway  (Kegg details for RBM28):
        Ribosome biogenesis in eukaryotes


    RBM28 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RBM28

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/141 Interacting proteins for RBM28 (Q9NW133 ENSP000002230734) via UniProtKB, MINT, STRING, and/or I2D (see all 141)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006397mRNA processing IEA--
    GO:0008380RNA splicing IEA--


    RBM28 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RBM28
    Search CenterWatch for drugs/clinical trials and news about RBM28 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RBM28 gene (2 alternative transcripts): 
    NM_001166135.1  NM_018077.2  

    Unigene Cluster for RBM28:

    RNA binding motif protein 28
    Hs.274263  [show with all ESTs]
    Unigene Representative Sequence: NM_018077
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000223073(uc003vmp.2 uc011koj.1 uc011kok.1) ENST00000481788
    ENST00000415472 ENST00000495327 ENST00000487602 ENST00000478061 ENST00000488249
    ENST00000459726

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    Inhib. RNA
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    Additional cDNA sequence: 

    AK001239.1 AK222716.1 AK300500.1 BC013889.2 

    6 DOTS entries:

    DT.213466  DT.100020924  DT.101961087  DT.102828804  DT.100811353  DT.91694955 

    24/102 AceView cDNA sequences (see all 102):

    BQ646473 BU609187 AL705640 AW801849 BQ654136 BM758471 BQ720067 AV654330 
    BM747080 BU194429 AI609293 BM766901 W76513 BU183334 BQ439480 BE880277 
    CA438299 BM823263 CR596407 AL134936 BM690082 BC013889 CR594156 CB995825 

    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for RBM28 (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b ^ 18a · 18b ^
    SP1:                                                                                                                                -     -     -               
    SP2:                    -                 -                                                                                                                     
    SP3:                                                                                                                                                            
    SP4:                                      -                                                                                                                     
    SP5:                    -                 -           -     -                                                                                                   

    ExUns: 19 ^ 20
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for RBM28

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RBM28 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ATGCTTCTTG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See RBM28 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RBM28

    SOURCE GeneReport for Unigene cluster: Hs.274263

    UniProtKB/Swiss-Prot: RBM28_HUMAN, Q9NW13
    Tissue specificity: Ubiquitously expressed

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for RBM28 gene from 8/34 species (see all 34)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia RBM286
    --
    47(a)
    1 ↔ 1
    GL343916.1(7362-27075)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.16812 Xenopus laevis transcribed sequence with weak similarity more 72.15(n)    BU909138.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc562582 hypothetical protein MGC56258 72.65(n)   393291  BC051781.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG48061 CG4806 50.06(n)
    38.93(a)
      37948  NM_138111.3  NP_611955.2 
    worm
    (Caenorhabditis elegans)
    Secernentea rbm-281 Protein RBM-28 48.4(n)
    37.61(a)
      175145  NM_064676.4  NP_497077.2 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes NOP4(YPL043W)4
    NOP41
    Nucleolar protein, essential for processing and maturation more4
    Nop4p1
    46.04(n)1
    34.53(a)1
      16(469939-471996)4
    8560631, 4  NP_015282.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT2G214401 RNA recognition motif-containing protein 46.68(n)
    34.02(a)
      816683  NM_127716.2  NP_565513.1 
    rice
    (Oryza sativa)
    Liliopsida AK067130.12   -- 77.12(n)    AK067130.1 


    ENSEMBL Gene Tree for RBM28 (if available)
    TreeFam Gene Tree for RBM28 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/664 NCBI SNPs in RBM28 are shown (see all 664    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 7 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs763205401,2
    C,--127333459(+) GCTCTC/TGCTTA 2 -- ds50013Minor allele frequency- T:0.13CSA WA 121
    rs171519871,2
    C,F,H,--127333524(+) GTTCAT/CACATA 2 -- ds500115Minor allele frequency- C:0.08NA NS EA WA 1222
    rs795486791,2
    C,F,--127333725(+) CTGGAT/CAATAT 2 -- ut311Minor allele frequency- C:0.02NA 120
    rs2022398531,2
    C--127333860(-) GAAAAC/TCCCCA 2 -- ut310--------
    rs732306341,2
    C,--127334433(+) TGTTCC/TCTCCC 2 -- int10--------
    rs69800011,2
    C,F,A,H,--127334476(+) CTATAC/ATATAG 2 -- int112Minor allele frequency- A:0.08NS EA NA WA 804
    rs69803011,2
    C,F,H,--127334523(+) CTTGAG/ATAGGA 2 -- int110Minor allele frequency- A:0.07NS EA NA WA 664
    rs1134780311,2
    --127335519(+) GATTGC/TGCCAT 2 -- int11Minor allele frequency- T:0.50CSA 2
    rs801281271,2
    F,--127335785(+) AGAAAA/GAAATG 2 -- int11Minor allele frequency- G:0.16WA 118
    rs1997427171,2
    C--127335823(-) AAGTCA/CCACAT 2 -- int10--------

    HapMap Linkage Disequilibrium report for RBM28 (127950436 - 127983962 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for RBM28
         2 CNVs: 59381 4558
    Human Gene Mutation Database (HGMD): RBM28

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RBM28
    DNA2.0 Custom Variant and Variant Library Synthesis for RBM28

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RBM28 for disorders           About GeneDecksing

    OMIM gene information: 612074   
    OMIM disorders: 612079  
    UniProtKB/Swiss-Prot: RBM28_HUMAN, Q9NW13
  • Defects in RBM28 are the cause of alopecia neurologic defects and endocrinopathy syndrome (ANES) [MIM:612079].
  • Affected individuals have hair loss of variable severity, ranging from complete alopecia to near-normal scalp hair
    with absence of body hair. All have moderate to severe mental retardation, progressive motor deterioration and central
    hypogonadotropic hypogonadism with delayed or absent puberty and central adrenal insufficiency. Additional features
    included short stature, microcephaly, gynecomastia, pigmentary anomalies, hypodontia, kyphoscoliosis, ulnar deviation
    of the hands, and loss of subcutaneous fat

    12 diseases for RBM28:    About MalaCards
    alopecia, neurologic defects, and endocrinopathy syndrome    alopecia    pituitary hormone deficiency    combined pituitary hormone deficiency
    treacher collins syndrome    short stature    hypodontia    gynecomastia
    hypogonadotropism    hypogonadism    microcephaly    malaria


    Export disorders for RBM28 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RBM28 gene, integrated from 9 sources (see all 29):
    (articles sorted by number of sources associating them with RBM28)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Alopecia, neurological defects, and endocrinopathy syndrome caused by decreased expression of RBM28, a nucleolar protein associated with ribosome biogenesis. (PubMed id 18439547)1, 2 Nousbeck J....Sprecher E. (2008)
    2. Human RBM28 protein is a specific nucleolar component of the spliceosomal snRNPs. (PubMed id 17081119)1, 2 Damianov A.... Bindereif A. (2006)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Functional proteomic analysis of human nucleolus. (PubMed id 12429849)1, 2 Scherl A.... Diaz J.-J. (2002)
    6. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    7. Functional proteomics establishes the interaction of S IRT7 with chromatin remodeling complexes and expands its role in regulation of R NA polymerase I transcription. (PubMed id 22586326)1 Tsai Y.C....Cristea I.M. (2012)
    8. A catalogue of putative HIV-1 protease host cell subst rates. (PubMed id 22944692)1 Impens F....Gevaert K. (2012)
    9. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    10. Integrative analysis of the ubiquitin proteome isolat ed using Tandem Ubiquitin Binding Entities (TUBEs). (PubMed id 22178446)1 Lopitz-Otsoa F....Rodriguez M.S. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 55131 HGNC: 21863 AceView: RBM28 Ensembl:ENSG00000106344 euGenes: HUgn55131
    ECgene: RBM28 Kegg: 55131 H-InvDB: RBM28

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RBM28 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for RBM28 gene:
    Search GeneIP for patents involving RBM28

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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