RBM22 Gene
protein-coding GIFtS: 52
GCID: GC05M150050
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RNA binding motif protein 22
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Aliases for RBM22 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| RNA Binding Motif Protein 221 2 | | RNA-Binding Motif Protein 222 3 | | ZC3H161 2 3 | | FLJ102901 | | Cwc21 2 | | Functional Spliceosome-Associated Protein 472 | | FSAP471 | | Pre-MRNA-Splicing Factor RBM222 | | Zinc Finger CCCH Domain-Containing Protein 162 3 | | |
Export aliases for RBM22 gene to outside databasesPrevious GC identifers: GC05M150099 GC05M145216 |
Summaries for RBM22 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| Entrez Gene summary for RBM22: This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved inpre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. (provided by RefSeq, Mar 2009) UniProtKB/Swiss-Prot: RBM22_HUMAN, Q9NW64Function: Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of theU6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of thespliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosoliccalcium-binding protein PDCD6 to the nucleus upon cellular stress responses Gene Wiki entry for RBM22
|
Genomic Views for RBM22 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000005.9 NC_018916.1 NT_029289.11
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the RBM22 gene promoter: AML1a Sp1 XBP-1 NF-kappaB E4BP4 PPAR-gamma1 PPAR-gamma2 NF-kappaB1 MRF-2 Other transcription factors
Search SABiosciences Chromatin IP Primers for RBM22
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RBM22 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 5q33.1 Ensembl cytogenetic band: 5q33.1 HGNC cytogenetic band: 5q33.1RBM22 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 5 GeneLoc Exon Structure GeneLoc location for GC05M150050: view genomic region
(about GC identifiers)
Start:
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150,070,352 bp from pter |
End:
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150,080,669 bp from pter |
Size:
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10,318 bases |
Orientation:
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minus strand |
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Proteins for RBM22 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: RBM22_HUMAN, Q9NW64 (See
protein sequence)Recommended Name: Pre-mRNA-splicing factor RBM22 Size: 420 amino acids; 46896 Da
Subunit: Identified in the spliceosome C complex. Interacts with PDCD6; the interaction induces translocation of PDCD6in the cytoplasm
Subcellular location: Nucleus. Cytoplasm. Note=Mainly located in the nucleus. Translocated from the nucleus to thecytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol
Sequence caution: Sequence=AAC99998.1; Type=Miscellaneous discrepancy; Note=Chimera;
1 PDB 3D structure from and Proteopedia for RBM22:2YTC (3D)
 
Secondary accessions: A6NDM5 B4DLI9 O95607Alternative splicing: 2 isoforms: Q9NW64-1 Q9NW64-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for RBM22: NX_Q9NW64
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9NW64 RBM22 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_060517.1 ENSEMBL proteins: ENSP00000199814 ENSP00000412118 ENSP00000428154 ENSP00000430946 ENSP00000441594 Human Recombinant Protein Products:
Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table
RBM22 for ontologies About GeneDecksing
RBM22 Antibody Products: Assay Products for RBM22: |
Protein
Domains / Families for RBM22 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RBM22 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9NW64ProtoNet protein and cluster: Q9NW64 2 Blocks protein families: IPB000504 RNA-binding region RNP-1 (RNA recognition motif) IPB000571 Zn-finger
UniProtKB/Swiss-Prot: RBM22_HUMAN, Q9NW64Domain: The C-terminus RRM domain and the zinc finger motif are necessary for RNA-binding (By similarity)Similarity: Belongs to the SLT11 familySimilarity: Contains 1 C3H1-type zinc fingerSimilarity: Contains 1 RRM (RNA recognition motif) domain |
Function for RBM22 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: RBM22_HUMAN, Q9NW64Function: Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of theU6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of thespliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosoliccalcium-binding protein PDCD6 to the nucleus upon cellular stress responses
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RBM22 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RBM22 (see all 2) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: RBM22 (NM_018047) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RBM22 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RBM22  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RBM22 |
Gene Ontology (GO): 5/8 molecular function terms (GO ID links to tree view) (see all 8): About this table
RBM22 for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for RBM22:
|
Pathways & Interactions for RBM22 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways  About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | mRNA Splicing - Major Pathway | |
Pathway sources See GeneCards unified pathways Show all pathways
1
Kegg Pathway (Kegg details for RBM22):
RBM22 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RBM22
STRING Interaction
Network Preview (showing 5 interactants - click image to see 21)
 5/30 Interacting proteins for RBM22 (Q9NW641, 2, 3 ENSP000001998144) via UniProtKB, MINT, STRING, and/or I2D (see all 30)About this table
Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6): About this table
RBM22 for ontologies About GeneDecksing
|
Drugs & Compounds for RBM22 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Browse Tocris compounds for RBM22 Search CenterWatch for drugs/clinical trials and news about RBM22 
|
Transcripts for RBM22 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for RBM22 gene: NM_018047.2 Unigene Cluster for RBM22: RNA binding motif protein 22 Hs.713564 [show with all ESTs]Unigene Representative Sequence: NM_0180479 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000520132 ENST00000199814(uc003lst.3) ENST00000447771 ENST00000522469 ENST00000518917 ENST00000521248 ENST00000521594 ENST00000521464 ENST00000540000
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RBM22 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RBM22 (see all 2) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: RBM22 (NM_018047) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RBM22 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RBM22  |
Additional cDNA sequence: AK001152.1 AK297019.1 AL136933.1 BC003402.1 12 DOTS entries: DT.453686 DT.100788221 DT.97856089 DT.92437478 DT.97781976 DT.70103058 DT.100656143 DT.120813644 DT.40132255 DT.120813639 DT.91641600 DT.97807821 24/239 AceView cDNA sequences (see all 239): BG389497 BQ218028 BF725444 BE218298 CB241453 H54707 AI499996 BG756631 BU170445 NM_018047 AA662903 BQ772802 BE673688 AK096728 AU135503 AL558737 AI337352 AA135874 CR594318 AW204355 AL538762 BX118278 BU152960 AA039404 GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for RBM22 About this scheme
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9 | ^ | 10 | ^ | 11 | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
ECgene alternative splicing isoforms for RBM22
|
Expression for RBM22 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RBM22 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: AAATTACTGT
About this image See RBM22 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for RBM22
SOURCE GeneReport for Unigene cluster: Hs.713564 SABiosciences Custom PCR Arrays for RBM22
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RBM22 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RBM22 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RBM22 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RBM22 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RBM22 |
Orthologs for RBM22 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for RBM22 gene from 9/35 species (see all 35) About this table
ENSEMBL Gene Tree for RBM22 (if available) TreeFam Gene Tree for RBM22 (if available)  |
Paralogs for RBM22 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for RBM22 gene
RBM22 for paralogs About GeneDecksing
5/15 Pseudogenes.org Pseudogenes for RBM22 (see all 15) PGOHUM00000248351 PGOHUM00000249060 PGOHUM00000249066 PGOHUM00000234908 PGOHUM00000243630
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Genomic Variants for RBM22 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 5 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
|---|
HapMap Linkage Disequilibrium report for RBM22 (150070352 - 150080669 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 3 variations for RBM22 3 CNVs: 69223 7477 3580  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RBM22 |
|
Disorders
/ Diseases for RBM22 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RBM22 for disorders About GeneDecksing
OMIM gene information: 612430
OMIM disorders: --
1 disease for RBM22: About MalaCardsmalaria Export disorders for RBM22 gene to outside databases
|
Publications for RBM22 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RBM22 gene, integrated from 9 sources (see all 30): (articles sorted by number of sources associating them with RBM22) | |  | Utopia: connect your pdf to the dynamic world of online information |
- The influence of calcium signaling on the regulation of alternative splicing. (PubMed id 19133299)1, 2, 3 Krebs J. (2008)
- Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. (PubMed id 22246180)1, 2 Rasche N....Luhrmann R. (2012)
- RNA-binding motif protein RBM22 is required for norma l development of zebrafish embryos. (PubMed id 20013661)1, 3 He F....Gou L.T. (2009)
- Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. (PubMed id 17045351)1, 2 Montaville P.... Krebs J. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. (PubMed id 11991638)1, 2 Jurica M.S.... Moore M.J. (2002)
- Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2 Wiemann S.... Poustka A. (2001)
- Transcriptional map of the Treacher Collins candidate gene region. (PubMed id 8681136)1, 2 Loftus S.K.... Wasmuth J.J. (1996)
- Proteome-wide identification of ubiquitylation sites b y conjugation of engineered lysine-less ubiquitin. (PubMed id 22053931)1 Oshikawa K....Nakayama K.I. (2012)
|
External Searches for RBM22 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing RBM22 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RBM22 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RBM22 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for RBM22 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for RBM22 gene: Search GeneIP for patents involving RBM22
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for RBM22 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
|
 | |
 | |
 |
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| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RBM22 | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RBM22 | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RBM22 | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RBM22 | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RBM22 | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RBM22 |
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| | | Search Tocris compounds for RBM22 |
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 | | RBM22 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RBM22 |
|  |  |  | | | Search ThermoFisher Antibodies for RBM22 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RBM22 |
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