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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RASIP1 Gene

protein-coding   GIFtS: 51
GCID: GC19M049223

Ras interacting protein 1

 Explore 1 disease affiliated with
RASIP1 via our new
 Human Malady Compendium 
Biological research products
for RASIP1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3

Aliases
Ras Interacting Protein 11 2
RAIN1 2 5
FLJ204011
Ras-Interacting Protein 12
Rain3

External Ids:    HGNC: 247161   Entrez Gene: 549222   Ensembl: ENSG000001055387   OMIM: 6096235   UniProtKB: Q5U6513   
ORGUL members:         
NONCODE:n333529    

Export aliases for RASIP1 gene to outside databases

Previous GC identifers: GC19M053916 GC19M053917 GC19M045604


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RAIN_HUMAN, Q5U651
Function: Required for the proper formation of vascular structures that develop via both vasculogenesis and
angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion,
which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho
GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in
endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote
HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000019.9  NC_018930.1  NT_011109.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RASIP1 gene promoter:
         p300   MyoD   STAT3   ATF   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRASIP1 promoter sequence
   Search SABiosciences Chromatin IP Primers for RASIP1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RASIP1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.33   Ensembl cytogenetic band:  19q13.33   HGNC cytogenetic band: 19q13.33

RASIP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RASIP1 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M049223:  view genomic region     (about GC identifiers)

Start:
49,223,842 bp from pter      End:
49,243,970 bp from pter
Size:
20,129 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RAIN_HUMAN, Q5U651 (See protein sequence)
Recommended Name: Ras-interacting protein 1  
Size: 963 amino acids; 103457 Da
Subunit: Interacts with Ras family members that have been activated by GTP binding. Interacts with HRAS, RAP1A, RAP2,
RRAS, RAF1 and RRAS2. Interacts with MYH9 and ARHGAP29 (By similarity)
Subcellular location: Cytoplasm, perinuclear region. Golgi apparatus, Golgi stack. Note=Associated with perinuclear
vesicles. Is recruited to Golgi stacks by activated HRAS
Secondary accessions: Q6U676

Explore the universe of human proteins at neXtProt for RASIP1: NX_Q5U651

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q5U651

  • RASIP1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_060275.2  
    ENSEMBL proteins: 
     ENSP00000222145  

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    Uscn Proteins for RASIP1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005795Golgi stack IEA--
    GO:0048471perinuclear region of cytoplasm IEA--


    RASIP1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RASIP1 for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR008984 SMAD_FHA_domain
     IPR018444 Dil_domain
     IPR002710 Dilute
     IPR000159 Ras-assoc
     IPR000253 FHA_dom

    Graphical View of Domain Structure for InterPro Entry Q5U651

    ProtoNet protein and cluster: Q5U651

    1 Blocks protein family: IPB000159 RA domain

    UniProtKB/Swiss-Prot: RAIN_HUMAN, Q5U651
    Similarity: Contains 1 dilute domain
    Similarity: Contains 1 Ras-associating domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RAIN_HUMAN, Q5U651
    Function: Required for the proper formation of vascular structures that develop via both vasculogenesis and
    angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion,
    which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho
    GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in
    endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote
    HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RASIP1

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding ----


    RASIP1 for ontologies           About GeneDecksing


    Animal Models:
         7 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Rasip1):
     cardiovascular system  cellular  embryogenesis  growth/size  homeostasis/metabolism 
     mortality/aging  no phenotypic analysis 

    RASIP1 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RASIP1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 8)

    5/8 Interacting proteins for RASIP1 (Q5U6513 ENSP000002221454) via UniProtKB, MINT, STRING, and/or I2D (see all 8)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HRASP011123, ENSP000003098454I2D: score=3 STRING: ENSP00000309845
    MRASO148073, ENSP000002891044I2D: score=1 STRING: ENSP00000289104
    RAP1AP628343, ENSP000003487864I2D: score=1 STRING: ENSP00000348786
    RAP2AP101143, ENSP000002453044I2D: score=2 STRING: ENSP00000245304
    RRASP103013, ENSP000002467924I2D: score=1 STRING: ENSP00000246792
    About this table

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001525angiogenesis ISS--
    GO:0001570vasculogenesis ISS--
    GO:0007165signal transduction IEA--
    GO:0010507negative regulation of autophagy IMP--
    GO:0032319regulation of Rho GTPase activity ISS--


    RASIP1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RASIP1

    1 HMDB Compound for RASIP1    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--
    Search CenterWatch for drugs/clinical trials and news about RASIP1 / RAIN 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RASIP1 gene: 
    NM_017805.2  

    Unigene Cluster for RASIP1:

    Ras interacting protein 1
    Hs.233955  [show with all ESTs]
    Unigene Representative Sequence: NM_017805
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000222145(uc002pkh.3 uc002pki.3)

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    hsa-miR-498 hsa-miR-137
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    Inhib. RNA
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    Additional cDNA sequence: 

    AK000408.1 AY378097.1 BC021860.1 BC028614.1 BC042111.1 

    4 DOTS entries:

    DT.207162  DT.121466014  DT.95187831  DT.95192882 

    24/68 AceView cDNA sequences (see all 68):

    AK000408 BE856162 AK123179 BF197429 AI143393 NM_017805 AI241453 AW772163 
    BF516076 BM694838 CR608210 BC021860 AW969501 BM906443 BX089325 AA503542 
    AI830353 BQ717077 NM_182575 R80760 AI951738 AI018800 AI808410 BC050460 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for RASIP1    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14a · 14b
    SP1:                          -                                   -                                       
    SP2:                                                                                                      
    SP3:                                                                                      -               
    SP4:                          -           -     -     -     -     -                                       
    SP5:                                                                                                      


    ECgene alternative splicing isoforms for RASIP1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RASIP1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGCTTAGGAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    RASIP1 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 5 LifeMap Cells 
    NameCategory
    PureStem™ endothelial progenitor 30-MV2-12 (Embryonic Progenitor Cell)Blood, Endothelium
    PureStem™ endothelial Progenitor 30-SM2-3 (Embryonic Progenitor Cell)Blood, Endothelium
    PureStem™ endothelial progenitor 30-SM2-1 (Embryonic Progenitor Cell)Endothelium
    PureStem™ endothelial progenitor RP1-MV2-18 (Embryonic Progenitor Cell)Endothelium
    PureStem™ endothelial progenitor 30-MV2-14 (Embryonic Progenitor Cell)Endothelium
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See RASIP1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RASIP1

    SOURCE GeneReport for Unigene cluster: Hs.233955

    UniProtKB/Swiss-Prot: RAIN_HUMAN, Q5U651
    Tissue specificity: Highly expressed in heart. Detected at lower levels in placenta and pancreas

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for RASIP1 gene from 3/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Rasip11 , 5 Ras interacting protein 11, 5 86(n)1
    95.08(a)1
      7 (29.39 cM)5
    699031  NM_028544.11  NP_082820.11 
     456274885 
    lizard
    (Anolis carolinensis)
    Reptilia RASIP16
    --
    58(a)
    1 ↔ 1
    GL343279.1(1151012-1165604)
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1001511301 ras-interacting protein 1-like 53.48(n)
    48.21(a)
      100151130  XM_001921544.3  XP_001921579.3 


    ENSEMBL Gene Tree for RASIP1 (if available)
    TreeFam Gene Tree for RASIP1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RASIP1 gene
    RADIL2  
    1 SIMAP similar gene for RASIP1 using alignment to 4 protein entries:     RAIN_HUMAN (see all proteins):
    RADIL

    RASIP1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/342 NCBI SNPs in RASIP1 are shown (see all 342    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1872434421,2
    --49223407(+) GAGAGA/GGATGG 2 -- us2k1 ds50010--------
    rs754659281,2
    C,--49223498(+) GGCGGG/TGTGCC 2 -- ds5001 us2k10--------
    rs1169530791,2
    F,--49223528(+) GAGGGG/TGCTGG 2 -- us2k1 ds50011Minor allele frequency- T:0.03EA 120
    rs5463081,2
    C,F,A,H,--49223570(+) GGGCTG/AGAGTA 2 -- ds5001 us2k110Minor allele frequency- A:0.42NA WA CSA EA 374
    rs6295041,2
    C,F,A,H,--49223633(+) GAGGGC/GCTTAT 2 -- ds5001 us2k112Minor allele frequency- G:0.50MN NA WA CSA EA 559
    rs1918117061,2
    --49223672(+) TCTGAG/TGGGTG 2 -- ds5001 us2k10--------
    rs1388537371,2
    --49223749(+) TACCTC/TCTCAG 2 -- us2k1 ds50010--------
    rs747988521,2
    C,F,--49223817(+) AGCGCC/ACCTGA 2 -- us2k1 ds50011Minor allele frequency- A:0.08WA 118
    rs1420731831,2
    --49223831(+) TTAACA/TATCGA 2 -- us2k1 ds50010--------
    rs1153515741,2
    C,F,--49223853(+) TACCAC/TGGATA 2 -- ut31 us2k11Minor allele frequency- T:0.02WA 118

    HapMap Linkage Disequilibrium report for RASIP1 (49223842 - 49243970 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 4 variations for RASIP1
         4 CNVs: 89277 78223 78222 32261

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RASIP1
    DNA2.0 Custom Variant and Variant Library Synthesis for RASIP1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RASIP1 for disorders           About GeneDecksing

    OMIM gene information: 609623    OMIM disorders: --

    1 disease for RASIP1:    About MalaCards
    crohn's disease

    Human Genome Epidemiology (HuGE) Navigator: RASIP1 (2 documents)

    Export disorders for RASIP1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RASIP1 gene, integrated from 9 sources (see all 11):
    (articles sorted by number of sources associating them with RASIP1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification and characterization of Rain, a novel Ras-interacting protein with a unique subcellular localization. (PubMed id 15031288)1, 2, 3, 9 Mitin N.Y....Taparowsky E.J. (2004)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. (PubMed id 22354037)2 McKnight N.C.... Tooze S.A. (2012)
    4. Large-scale genome-wide association analysis of bipol ar disorder identifies a new susceptibility locus near ODZ4. (PubMed id 21926972)1 Sklar P....Purcell S.M. (2011)
    5. Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
    6. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    7. Four novel Loci (19q13, 6q24, 12q24, and 5q14) influe nce the microcirculation in vivo. (PubMed id 21060863)1 Ikram M.K....Wong T.Y. (2010)
    8. Genome-wide meta-analysis increases to 71 the number of confirmed Crohn's disease susceptibility loci. (PubMed id 21102463)1 Franke A....Parkes M. (2010)
    9. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1 Ota T.... Sugano S. (2004)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54922 HGNC: 24716 AceView: RASIP1andMGC34799 Ensembl:ENSG00000105538 euGenes: HUgn54922
    ECgene: RASIP1 H-InvDB: RASIP1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RASIP1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RASIP1 gene:
    Search GeneIP for patents involving RASIP1

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