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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RASAL2 Gene

protein-coding   GIFtS: 53
GCID: GC01P178063

RAS protein activator like 2

 Explore 5 diseases affiliated with
RASAL2 via our new
 Human Malady Compendium 
Biological research products
for RASAL2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
RAS Protein Activator Like 21 2
NGAP2 3
RAS Protein Activator-Like 22 3
NGAP2 3
Ras GTPase-Activating Protein NGAP2
Ras Protein Activator Like 12

External Ids:    HGNC: 98741   Entrez Gene: 94622   Ensembl: ENSG000000753917   OMIM: 6061365   UniProtKB: Q9UJF23   

Export aliases for RASAL2 gene to outside databases

Previous GC identifers: GC01P175834 GC01P173491 GC01P174643 GC01P175303 GC01P174795 GC01P176329 GC01P149291


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RASAL2:
This gene encodes a protein that contains the GAP-related domain (GRD), a characteristic domain of GTPase-activating
proteins (GAPs). GAPs function as activators of Ras superfamily of small GTPases. The protein encoded by this gene is
able to complement the defective RasGAP function in a yeast system. Two alternatively spliced transcript variants of
this gene encoding distinct isoforms have been reported. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: NGAP_HUMAN, Q9UJF2
Function: Inhibitory regulator of the Ras-cyclic AMP pathway

Gene Wiki entry for RASAL2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_004487.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RASAL2 gene promoter:
         Nkx2-2   p300   NRSF form 1   NRSF form 2   HNF-1A   E47   HFH-1   Zic1   Sox9   Hlf   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRASAL2 promoter sequence
   Search SABiosciences Chromatin IP Primers for RASAL2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RASAL2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q24   Ensembl cytogenetic band:  1q25.2   HGNC cytogenetic band: 1q25

RASAL2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RASAL2 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P178063:  view genomic region     (about GC identifiers)

Start:
178,062,864 bp from pter      End:
178,448,649 bp from pter
Size:
385,786 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NGAP_HUMAN, Q9UJF2 (See protein sequence)
Recommended Name: Ras GTPase-activating protein nGAP  
Size: 1139 amino acids; 128558 Da
Sequence caution: Sequence=AAI10612.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: F8W755 O95174 Q2TB22 Q5TFU9
Alternative splicing: 2 isoforms:  Q9UJF2-1   Q9UJF2-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for RASAL2: NX_Q9UJF2

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9UJF2

  • RASAL2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_004832.1  NP_733793.2  

    ENSEMBL proteins: 
     ENSP00000356621   ENSP00000420558   ENSP00000402897   ENSP00000407768  

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    Uscn Proteins for RASAL2

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005737cytoplasm IBA--
    GO:0031235intrinsic to internal side of plasma membrane IBA--


    RASAL2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RASAL2 for domains           About GeneDecksing

    5/7 InterPro domains/families (see all 7):
     IPR001936 RasGAP
     IPR008936 Rho_GTPase_activation_prot
     IPR001849 Pleckstrin_homology
     IPR023152 RasGAP_CS
     IPR021887 DUF3498

    Graphical View of Domain Structure for InterPro Entry Q9UJF2

    ProtoNet protein and cluster: Q9UJF2

    3 Blocks protein families:
    IPB000008 C2 domain
    IPB001849 Pleckstrin-like
    IPB001936 Ras GTPase-activating protein


    UniProtKB/Swiss-Prot: NGAP_HUMAN, Q9UJF2
    Similarity: Contains 1 C2 domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 Ras-GAP domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: NGAP_HUMAN, Q9UJF2
    Function: Inhibitory regulator of the Ras-cyclic AMP pathway

    miRNA
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    hsa-miR-323-3p hsa-miR-520f hsa-miR-3607-3p hsa-miR-4254 hsa-miR-30d hsa-miR-371-5p hsa-miR-342-3p hsa-miR-3916
    SwitchGear 3'UTR luciferase reporter plasmidRASAL2 3' UTR sequence
    Inhib. RNA
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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005096GTPase activator activity ----
    GO:0005099Ras GTPase activator activity IBA--
    GO:0005543phospholipid binding IEA--


    RASAL2 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for RASAL2:
     Lamellipodia cells 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1TNF-alpha/NF-kB Signaling Pathway
    TNF-alpha/NF-kB Signaling Pathway1.00


    1 BioSystems Pathway for RASAL2 
        TNF-alpha/NF-kB Signaling Pathway


    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RASAL2

    4 Interacting proteins for RASAL2 (Q9UJF22, 3) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    YWHAGP619812, 3MINT-50873 I2D: score=3 
    NFKBIBQ156532, 3MINT-47863 I2D: score=2 
    MCCP235083I2D: score=1 
    --Q9WMX22MINT-7050824 MINT-7049543
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165signal transduction IEA--
    GO:0032320positive regulation of Ras GTPase activity IBA--
    GO:0046580negative regulation of Ras protein signal transduction IBA--
    GO:0051056regulation of small GTPase mediated signal transduction ----


    RASAL2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RASAL2
    Search CenterWatch for drugs/clinical trials and news about RASAL2 / NGAP 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RASAL2 gene (2 alternative transcripts): 
    NM_004841.3  NM_170692.2  

    Unigene Cluster for RASAL2:

    RAS protein activator like 2
    Hs.496139  [show with all ESTs]
    Unigene Representative Sequence: NM_170692
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000367649 ENST00000465723 ENST00000462775 ENST00000433130 ENST00000463079
    ENST00000448150(uc009wxb.2 uc001glq.3 uc001glr.3 uc009wxc.3)


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    8/119 QIAGEN miScript miRNA Assays for microRNAs that regulate RASAL2 (see all 119):
    hsa-miR-323-3p hsa-miR-520f hsa-miR-3607-3p hsa-miR-4254 hsa-miR-30d hsa-miR-371-5p hsa-miR-342-3p hsa-miR-3916
    SwitchGear 3'UTR luciferase reporter plasmidRASAL2 3' UTR sequence
    Inhib. RNA
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RASAL2
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    Additional cDNA sequence: 

    AB209789.1 AF047711.1 AK022662.1 AK075169.1 AK290308.1 AK311017.1 BC030292.1 BC063442.1 
    BC078167.1 BC110611.2 

    14 DOTS entries:

    DT.113098  DT.97813719  DT.111525  DT.121379497  DT.40299243  DT.40192156  DT.101966292  DT.405958 
    DT.97827245  DT.95181045  DT.92421965  DT.95158925  DT.99988305  DT.91908428 

    1 AceView cDNA sequence:

    AB007970 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RASAL2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GAGTGCAATA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    RASAL2 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Intermediate limb mesenchymal cells (ILM cells) (Primary Cell)Bone, Cartilage, Limb
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See RASAL2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RASAL2

    SOURCE GeneReport for Unigene cluster: Hs.496139
        SABiosciences Custom PCR Arrays for RASAL2
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RASAL2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for RASAL2 gene from 6/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves RASAL21 RAS protein activator like 2 81.81(n)
    91.36(a)
      429069  XM_426625.3  XP_426625.3 
    lizard
    (Anolis carolinensis)
    Reptilia RASAL26
    --
    83(a)
    1 ↔ 1
    GL343300.1(436098-514076)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.15752 Xenopus laevis transcribed sequence with moderate similarity more 78.32(n)    BX845447.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.244172 Transcribed sequence with weak similarity to protein more 77.76(n)    57052258 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG426846
    --
    16(a)
    1 → many
    X(17604178-17645718)
    worm
    (Caenorhabditis elegans)
    Secernentea gap-23 GTPase-activating protein 44(a)   X(9507707-9551128)   --


    ENSEMBL Gene Tree for RASAL2 (if available)
    TreeFam Gene Tree for RASAL2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RASAL2 gene
    SYNGAP12  DAB2IP2  RASA42  RASA12  RASAL32  RASA22  RASA4B2  RASA32  
    RASAL12  
    4 SIMAP similar genes for RASAL2 using alignment to 4 protein entries:     NGAP_HUMAN (see all proteins):
    DAB2IP    SYNGAP1    FLJ00087    RASAL3

    RASAL2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/5355 NCBI SNPs in RASAL2 are shown (see all 5355    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1841853361,2
    --178060921(+) ACAGGG/TAGGGT 2 -- nc-transcript-variantus2k10--------
    rs1888406861,2
    --178061006(+) GCAGGA/GCTTAA 2 -- us2k1 nc-transcript-variant0--------
    rs1141260071,2
    --178061156(+) AAAAAA/CAAATA 2 -- nc-transcript-variantus2k11Minor allele frequency- C:0.01WA 118
    rs1406901771,2
    --178061192(+) ACTGGA/CGGTTG 2 -- us2k1 nc-transcript-variant0--------
    rs775064381,2
    --178061457(+) GTGCTA/GAGCCT 2 -- nc-transcript-variantus2k10--------
    rs1815944601,2
    --178061474(+) CTCAGC/TACTCA 2 -- us2k1 nc-transcript-variant0--------
    rs412671721,2
    --178061570(+) AAGCAA/GGCAGG 2 -- us2k1 nc-transcript-variant1Minor allele frequency- G:0.01NA 120
    rs2021842641,2
    C--178061613(+) ACCATA/CANNNN 2 -- us2k1 nc-transcript-variant0--------
    rs1417918691,2
    --178061615(+) CATCAA/TGCCAC 2 -- us2k1 nc-transcript-variant0--------
    rs1454115141,2
    --178061945(+) TGCAAC/TGTAGG 2 -- us2k1 nc-transcript-variant0--------

    HapMap Linkage Disequilibrium report for RASAL2 (178062864 - 178312864 bp, first 250kb of RASAL2)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 3 variations for RASAL2
         3 CNVs: 97565 97564 84672

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RASAL2 for disorders           About GeneDecksing

    OMIM gene information: 606136    OMIM disorders: --

    5 diseases for RASAL2:    About MalaCards
    prostate cancer    prostatitis    asthma    obesity
    neuronitis

    Human Genome Epidemiology (HuGE) Navigator: RASAL2 (2 documents)

    Export disorders for RASAL2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RASAL2 gene, integrated from 9 sources (see all 24):
    (articles sorted by number of sources associating them with RASAL2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A novel human RasGAP-like gene that maps within the prostate cancer susceptibility locus at chromosome 1q25. (PubMed id 9877179)1, 2, 3 Noto S....Hatakeyama M. (1998)
    2. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
    3. Genome-wide association study of antibody response to smallpox vaccine. (PubMed id 22542470)1 Ovsyannikova I.G....Poland G.A. (2012)
    4. ECT2 and RASAL2 mediate mesenchymal-amoeboid transitio n in human astrocytoma cells. (PubMed id 22683310)1 Weeks A....Rutka J.T. (2012)
    5. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    6. System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
    7. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    8. Evaluating the discriminative power of multi-trait ge netic risk scores for type 2 diabetes in a northern Swedish population. (PubMed id 20571754)1 Fontaine-Bisson B....Franks P.W. (2010)
    9. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
    10. Analyses of shared genetic factors between asthma and obesity in children. (PubMed id 20816195)1 MelAcn E....Lasky-Su J. (2010)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 9462 HGNC: 9874 AceView: RASAL2.3 Ensembl:ENSG00000075391 euGenes: HUgn9462
    ECgene: RASAL2 H-InvDB: RASAL2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RASAL2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RASAL2 gene:
    Search GeneIP for patents involving RASAL2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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