Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

RAPGEF3 Gene

protein-coding GIFtS: 64
GCID: GC12M048128

Rap guanine nucleotide exchange factor (GEF) 3

(Previous name: RAP guanine-nucleotide-exchange factor (GEF) 3 )

Explore 17 diseases affiliated with
RAPGEF3 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Rap Guanine Nucleotide Exchange Factor (GEF) 3¹ ²		EPAC 1² ³
EPAC¹ ² ³ ⁵		Rap1 Guanine-Nucleotide-Exchange Factor Directly Activated By CAMP² ³
Bcm910¹		RAP Guanine-Nucleotide-Exchange Factor (GEF) 3¹
CAMP-GEFI²		9330170P05Rik²
Exchange Factor Directly Activated By CAMP 1² ³		CAMP-GEFI²
Exchange Protein Directly Activated By CAMP 1² ³		HSU79275²
EPAC1² ³		Rap Guanine Nucleotide Exchange Factor 3²
CAMP-Regulated Guanine Nucleotide Exchange Factor I² ³		CGEF1³

External Ids:	HGNC: 16629¹	Entrez Gene: 10411²	Ensembl: ENSG00000079337⁷	OMIM: 606057⁵	UniProtKB: O95398³

Export aliases for RAPGEF3 gene to outside databases

Previous GC identifers: GC12M046418 GC12M046419 GC12M045159

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RPGF3_HUMAN, O95398

Function: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.

Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates

the PI3K gamma complex and which is involved in angiogenesis

summary for RAPGEF3:

GTPases are a group of enzymes that catalyze hydrolysis of the gamma phosphate bond in guanine triphosphate

(GTP) to form guanine diphosphate (GDP). Mg2+ is essential for catalytic activity. GTPases are often coupled

to G proteins and have essential roles in signal transduction, protein synthesis and translocation, control

of cell growth and differentiation and vesicular transport regulation.

Gene Wiki entry for RAPGEF3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000012.11 NC_018923.1 NT_029419.12

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the RAPGEF3 gene promoter:
Sp1 YY1 ATF
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: RAPGEF3 promoter sequence

Search SABiosciences Chromatin IP Primers for RAPGEF3

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAPGEF3

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.1 Ensembl cytogenetic band: 12q13.11 HGNC cytogenetic band: 12q13.1

RAPGEF3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RAPGEF3 gene location

GeneLoc information about chromosome 12 GeneLoc Exon Structure

GeneLoc location for GC12M048128: view genomic region (about GC identifiers)

Start:	48,128,453 bp from pter	End:	48,164,823 bp from pter
Size:	36,371 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RPGF3_HUMAN, O95398 (See protein sequence)

Recommended Name: Rap guanine nucleotide exchange factor 3

Size: 923 amino acids; 103751 Da

Subunit: Interacts with PDE3B

Subcellular location: Endomembrane system

Caution: It is uncertain whether Met-1 or Met-43 is the initiator

Sequence caution: Sequence=AAD02890.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Sequence=AAD12740.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Secondary accessions: O95634 Q8WVN0

Alternative splicing: 2 isoforms: O95398-1 O95398-2

Explore the universe of human proteins at neXtProt for RAPGEF3: NX_O95398

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_O95398

RAPGEF3 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (3 alternative transcripts):

NP_001092001.1 NP_001092002.1 NP_006096.2

ENSEMBL proteins:

ENSP00000395708 ENSP00000449905 ENSP00000448619 ENSP00000373864 ENSP00000448480

ENSP00000447691 ENSP00000378764 ENSP00000449818 ENSP00000446731 ENSP00000448804

ENSP00000380278 ENSP00000384521 ENSP00000171000 ENSP00000373863 ENSP00000438798

Reactome Protein details: O95398
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Proteins (see all 2): RAPGEF3 OriGene Protein Over-expression Lysate (see all 3): RAPGEF3 OriGene Custom Protein Services for RAPGEF3
	GenScript Custom Purified and Recombinant Proteins Services for RAPGEF3
	Novus Biologicals RAPGEF3 Proteins Novus Biologicals RAPGEF3 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for RAPGEF3

Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005622	intracellular	--	--
GO:0005624	membrane fraction	--	--
GO:0005886	plasma membrane	TAS	--
GO:0005952	cAMP-dependent protein kinase complex	IEA	--
GO:0012505	endomembrane system	IEA	--

RAPGEF3 for ontologies About GeneDecksing

RAPGEF3 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	Cell Signaling Technology (CST) Antibodies for RAPGEF3 (Epac1)
	OriGene Antibodies: RAPGEF3 OriGene Custom Antibody Services for RAPGEF3
	GenScript Custom Superior Antibodies Services for RAPGEF3
	Novus Biologicals RAPGEF3 Antibodies
	Abcam antibodies for RAPGEF3
	Uscn Antibodies for RAPGEF3
	ThermoFisher Antibodies for RAPGEF3

Assay Products for RAPGEF3:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for RAPGEF3
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for RAPGEF3

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

RAPGEF3 for domains About GeneDecksing

5/11 InterPro domains/families (see all 11):

IPR008937 Ras_GEF

IPR018490 cNMP-bd-like

IPR023578 Ras_GEF_dom

IPR001895 RasGRF_CDC25

IPR000595 cNMP-bd_dom

Graphical View of Domain Structure for InterPro Entry O95398

ProtoNet protein and cluster: O95398

4 Blocks protein families:

IPB000591 Pleckstrin putative G-protein interacting domain

IPB000595 Cyclic nucleotide-binding domain

IPB001895 Guanine-nucleotide dissociation stimulator CDC25

IPB002373 cAMP-dependent protein kinase signature

UniProtKB/Swiss-Prot: RPGF3_HUMAN, O95398

Domain: The DEP domain is involved in membrane localization independent from regulation by cAMP

Similarity: Contains 1 cyclic nucleotide-binding domain

Similarity: Contains 1 DEP domain

Similarity: Contains 1 N-terminal Ras-GEF domain

Similarity: Contains 1 Ras-GEF domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: RPGF3_HUMAN, O95398

Function: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.

Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates

the PI3K gamma complex and which is involved in angiogenesis

miRNA Products:		OriGene 3'-UTR Clone (see all 3): RAPGEF3 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RAPGEF3
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate RAPGEF3 (see all 14):
		hsa-miR-345 hsa-miR-1178 hsa-miR-1266 hsa-miR-3194-5p hsa-miR-550a hsa-miR-539 hsa-miR-4268 hsa-miR-513a-5p

		SwitchGear 3'UTR luciferase reporter plasmid: RAPGEF3 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAPGEF3 (see all 7) OriGene shRNA RFP: RAPGEF3 OriGene siRNA: RAPGEF3
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAPGEF3

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for RAPGEF3

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAPGEF3 (see all 7) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAPGEF3 (see all 4) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 3): RAPGEF3 (NM_001098531)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for RAPGEF3
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAPGEF3

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for RAPGEF3
		Search LifeMap BioReagents cell lines for RAPGEF3

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAPGEF3

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005085	guanyl-nucleotide exchange factor activity	IEA	--
GO:0005515	protein binding	IPI	--
GO:0008603	cAMP-dependent protein kinase regulator activity	IEA	--
GO:0030552	cAMP binding	IEA	--

RAPGEF3 for ontologies About GeneDecksing

1 GenomeRNAi human phenotype for RAPGEF3:

Increased telomerase activity

Animal Models:
Mouse knock-out Rapgef3^tm1Utak for RAPGEF3
3 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Rapgef3):

cellular

nervous system

no phenotypic analysis

RAPGEF3 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/18 super-pathways (see all 18) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Development_A2B receptor- action via G-protein alpha s

	Development A2B receptor: action via G-protein alpha s	1.00			Development_A2A receptor signaling	0.43
	Development_A2B receptor- action via G-protein alpha s	1.00			Development A2A receptor signaling	0.43

G-protein signaling_G-Protein alpha-i signaling cascades

	Development Beta-adrenergic receptors regulation of ERK	0.55			G-protein signaling_G-Protein alpha-s signaling cascades	0.44
	Development_Beta-adrenergic receptors regulation of ERK	0.55			G-protein signaling G-Protein alpha-s signaling cascades	0.44

Regulation of Insulin Secretion

	Regulation of Insulin Secretion	1.00			Regulation of Insulin Secretion by Glucagon-like Peptide-1	0.55
	Integration of energy metabolism	0.74

Platelet activation, signaling and aggregation

Platelet activation, signaling and aggregation

1.00

Hemostasis

0.43

Immune System

Immune System

1.00

Adaptive Immune System

0.59

Pathway sources
See GeneCards unified pathways
Show all pathways

5/7 EMD Millipore Pathways for RAPGEF3 (see all 7)

	Development Beta-adrenergic receptors regulation of ERK
	Development A2A receptor signaling
	G-protein signaling G-Protein alpha-s signaling cascades
	Signal transduction cAMP signaling
	Development G-Proteins mediated regulation MARK-ERK signaling

2 Cell Signaling Technology (CST) Pathways for RAPGEF3

	Ca, cAMP and Lipid Signaling
	Cytoskeletal Signaling

5/7 GeneGo (Thomson Reuters) Pathways for RAPGEF3 (see all 7)

	G-protein signaling G-Protein alpha-s signaling cascades
	Development A2A receptor signaling
	G-protein signaling Rap1A regulation pathway
	Development A2B receptor- action via G-protein alpha s
	Signal transduction cAMP signaling

2 BioSystems Pathways for RAPGEF3

	Integrated Pancreatic Cancer Pathway
	Integrated Pancreatic Cancer Pathway

5/13 Reactome Pathways for RAPGEF3 (see all 13)

	Hemostasis
	Integrin cell surface interactions
	Metabolism
	Regulation of Insulin Secretion
	Adaptive Immune System

2 Kegg Pathways (Kegg details for RAPGEF3):

	Leukocyte transendothelial migration
	Long-term potentiation

RAPGEF3 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for RAPGEF3

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/27 Interacting proteins for RAPGEF3 (O95398^{1, 2, 3} ENSP00000395708⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 27)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
RAP1B	P61224²^,³, ENSP00000250559⁴	MINT-6741806 I2D: score=1 STRING: ENSP00000250559
RAP1A	P62834³, ENSP00000348786⁴	I2D: score=1 STRING: ENSP00000348786
PDE3B	Q13370¹, ENSP00000282096⁴	EBI-6172806,EBI-6172856 STRING: ENSP00000282096
GNAI1	ENSP00000343027⁴	STRING: ENSP00000343027
GNAI2	ENSP00000312999⁴	STRING: ENSP00000312999

About this table

Gene Ontology (GO): 5/11 biological process terms (GO ID links to tree view) (see all 11): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001525	angiogenesis	IEA	--
GO:0006112	energy reserve metabolic process	TAS	--
GO:0007165	signal transduction	TAS	9853756
GO:0007264	small GTPase mediated signal transduction	IEA	--
GO:0007596	blood coagulation	TAS	--

RAPGEF3 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

RAPGEF3 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Enzo Life Sciences drugs & compounds for RAPGEF3

Compounds for RAPGEF3 available from Tocris Bioscience About this table

Compound	Action	CAS #
8CPT-2Me-cAMP, sodium salt	Selective Epac activator	[510774-50-2]

4 HMDB Compounds for RAPGEF3 About this table

Compound	Synonyms	CAS #	PubMed Ids
Cyclic AMP	Cyclic AMP (see all 19)	60-92-4	--
Guanosine diphosphate	5'-GDP (see all 10)	146-91-8	--
Guanosine monophosphate	5'-GMP (see all 14)	85-32-5	--
Guanosine triphosphate	5'-GTP (see all 10)	86-01-1	--

10/15 Novoseek chemical compound relationships for RAPGEF3 gene (see all 15) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
cyclic amp	70	78	15591041 (5), 15525573 (4), 18391018 (3), 19307703 (3) (see all 23)
forskolin	69.3	9	17565009 (2), 15525573 (2), 16172130 (2), 12960055 (1) (see all 7)
ryanodine	41.3	1	18957419 (1)
guanine	38.3	3	17993260 (1), 16269655 (1), 12819211 (1)
calcium	37	25	18957419 (3), 16837018 (2), 11292831 (1), 17957381 (1) (see all 11)
adenylate	35.1	1	17284589 (1)
gdp	33.8	5	19546222 (2), 14615375 (1), 16728394 (1)
gtp	31.9	13	18495429 (2), 12783872 (1), 19546222 (1), 17178726 (1)
pge1	26.8	3	15967167 (2)
phosphatidylinositol	21.7	2	11801596 (1), 12960055 (1)

Search CenterWatch for drugs/clinical trials and news about RAPGEF3 / RPGF3

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for RAPGEF3 gene (3 alternative transcripts):

NM_001098531.2 NM_001098532.2 NM_006105.5

Unigene Cluster for RAPGEF3:

Rap guanine nucleotide exchange factor (GEF) 3

Hs.8578 [show with all ESTs]

Unigene Representative Sequence: NM_001098531

18/24 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 24):

ENST00000449771 ENST00000547856 ENST00000549151 ENST00000395360 ENST00000482843

ENST00000479866 ENST00000488250 ENST00000389212 ENST00000548919 ENST00000490387

ENST00000548434 ENST00000473777 ENST00000476259 ENST00000395358(uc001rqa.3)

ENST00000494764 ENST00000495465 ENST00000466322 ENST00000495953

miRNA Products:		OriGene 3'-UTR Clone (see all 3): RAPGEF3 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RAPGEF3
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate RAPGEF3 (see all 14):
		hsa-miR-345 hsa-miR-1178 hsa-miR-1266 hsa-miR-3194-5p hsa-miR-550a hsa-miR-539 hsa-miR-4268 hsa-miR-513a-5p

		SwitchGear 3'UTR luciferase reporter plasmid: RAPGEF3 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAPGEF3 (see all 7) OriGene shRNA RFP: RAPGEF3 OriGene siRNA: RAPGEF3
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAPGEF3

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAPGEF3 (see all 7) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAPGEF3 (see all 4) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 3): RAPGEF3 (NM_001098531)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for RAPGEF3
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAPGEF3

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAPGEF3 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat RAPGEF3
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAPGEF3
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAPGEF3

Additional cDNA sequence:

AF103905.1 AK092448.1 AK125545.1 AK290230.1 AK299029.1 AK308079.1 BC017728.1 BC092404.1

U78168.1 U78169.1 U79275.1

12 DOTS entries:

DT.101963950 DT.92016399 DT.410511 DT.121171772 DT.100744578 DT.111018 DT.101942 DT.95166221

DT.102843210 DT.435797 DT.121171795 DT.95166219

24/91 AceView cDNA sequences (see all 91):

AW003615 CD515786 AW801798 BX115615 BQ007070 BE394226 BI000902 BU728130

BU151157 BG822090 AK055502 BQ711010 BQ775848 NM_006105 CA427944 BP349451

BX509905 R49966 AL598685 BI770347 BE392782 BI037765 CA976002 BG005372

GeneLoc Exon Structure

5/11 Alternative Splicing Database (ASD) splice patterns (SP) for RAPGEF3 (see all 11) About this scheme

ExUns:	1a	·	1b	^	2	^	3a	·	3b	^	4	^	5	^	6	^	7a	·	7b	·	7c	^	8	^	9	^	10a	·	10b	^	11	^	12a	·	12b	^	13	^	14	^	15	^	16	^	17	^	18a	·	18b	^	19	^
SP1:					-		-																										-														-
SP2:					-		-																																								-
SP3:											-		-		-		-		-		-												-														-
SP4:
SP5:

ExUns:	20a	·	20b	·	20c	^	21	^	22	^	23	^	24	^	25	^	26	^	27	^	28a	·	28b	^	29a	·	29b	·	29c	·	29d
SP1:	-		-
SP2:	-		-						-
SP3:	-		-
SP4:	-		-
SP5:

ECgene alternative splicing isoforms for RAPGEF3

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

RAPGEF3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AGGAGGGGCT

About this image

RAPGEF3 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

1 LifeMap In Vivo Development Anatomical Compartment/Cell

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Heart

Endocardium

Endocardial Tube Cells

Endocardium

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See RAPGEF3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RAPGEF3

SOURCE GeneReport for Unigene cluster: Hs.8578

UniProtKB/Swiss-Prot: RPGF3_HUMAN, O95398

Tissue specificity: Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney

SABiosciences Custom PCR Arrays for RAPGEF3

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAPGEF3 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat RAPGEF3
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAPGEF3
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAPGEF3

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAPGEF3

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for RAPGEF3 gene from 5/18 species (see all 18) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	RAPGEF3¹	Rap guanine nucleotide exchange factor (GEF) 3	70.06(n) 68.38(a)		426888 XM_424494.3 XP_424494.3
lizard (Anolis carolinensis)	Reptilia	RAPGEF3⁶	--	68(a)	1 ↔ 1	GL343252.1(1275826-1327162)
zebrafish (Danio rerio)	Actinopterygii	rapgef3⁶	Rap guanine nucleotide exchange factor (GEF) 3	54(a)	1 ↔ 1	23(33588106-33657726)
fruit fly (Drosophila melanogaster)	Insecta	Epac⁶	Epac	36(a)	1 → many	2R(2647220-2686446)
worm (Caenorhabditis elegans)	Secernentea	epac-1⁶	Rap guanine nucleotide exchange factor 1	28(a)	1 → many	III(10189127-10195308)

ENSEMBL Gene Tree for RAPGEF3 (if available)
TreeFam Gene Tree for RAPGEF3 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for RAPGEF3 gene

RAPGEF2² RAPGEFL1² RAPGEF6² RAPGEF4² RASGEF1C² RASGEF1A² RAPGEF5² RASGEF1B²

12 SIMAP similar genes for RAPGEF3 using alignment to 12 protein entries: RPGF3_HUMAN (see all proteins):

DKFZp781H1715 RAPGEF4 Nbla00496 CGEF2 RAPGEF5 RAPGEF2

RAPGEFL1 DKFZp586O1422 KIA001LB PDZGEF1 RAPGEF6 FNIP1

RAPGEF3 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 12 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs144373254^1,2		--	48127953(+)	`GGTGCA/GCACCA`	3	--	int¹		0	--	--	--	--
rs188341801^1,2		--	48127963(+)	`ACCATA/TCCCGG`	3	--	int¹		0	--	--	--	--
rs148773703^1,2		--	48127964(+)	`CCATAC/TCCGGG`	3	--	int¹		0	--	--	--	--
rs141497754^1,2		--	48127965(+)	`CATACC/TCGGGT`	3	--	int¹		0	--	--	--	--
rs757284^1,2	C,F,A,H,	--	48128119(-)	`ACGTAC/GTTACT`	3	--	int¹		37		MN NS EA NA WA CSA	3668
rs193201670^1,2		--	48128123(+)	`AGTACA/GTGAAA`	3	--	int¹		0	--	--	--	--
rs11168213^1,2	C,F,H,	--	48128186(+)	`CTAATC/ACTGCA`	3	--	int¹		16		NS EA NA WA	1640
rs10875683^1,2	C,F,A,H,	--	48128283(+)	`CTTCAC/GCTGTC`	3	--	int¹		27		NS EA NA WA CSA	2770
rs150874763^1,2		--	48128304(+)	`TTAAGC/TAGTTA`	3	--	int¹		0	--	--	--	--
rs140433170^1,2		--	48128323(+)	`TGGGCA/GAACCT`	3	--	int¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for RAPGEF3 (48128453 - 48164823 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for RAPGEF3: --

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAPGEF3

DNA2.0 Custom Variant and Variant Library Synthesis for RAPGEF3

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

RAPGEF3 for disorders           About GeneDecksing

OMIM gene information: 606057    OMIM disorders: --

17 diseases for RAPGEF3:    About MalaCards

nicotine dependence acute lymphoblastic leukemia lymphoblastic leukemia follicular adenoma

alzheimer's disease melanoma metastasis thrombocytopenia adenoma

gingivitis pancreatic carcinoma leukemia melanoma

pancreatitis thyroiditis carcinoma prostatitis

neuronitis

4 Novoseek disease relationships for RAPGEF3 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
hypertrophy	6.72	3	18323524 (1), 19307703 (1), 16269655 (1)
inflammation	0	2	17068197 (1), 18176800 (1)
tumors	0	1	18172027 (1)
alzheimers disease	0	1	16246111 (1)

Human Genome Epidemiology (HuGE) Navigator: RAPGEF3 (3 documents)

Export disorders for RAPGEF3 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for RAPGEF3 gene, integrated from 9 sources (see all 194):
(articles sorted by number of sources associating them with RAPGEF3)

Utopia: connect your pdf to the dynamic
world of online information

Mechanism of regulation of the Epac family of cAMP-dependent RapGEFs. (PubMed id 10777494)^{1, 2, 3} de Rooij J....Bos J.L. (2000)
A family of cAMP-binding proteins that directly activate rap1. (PubMed id 9856955)^{1, 2, 3} Kawasaki H.... Graybiel A.M. (1998)
Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. (PubMed id 9853756)^{1, 2, 9} de Rooij J.... Bos J.L. (1998)
A phosphodiesterase 3B-based signaling complex integr ates exchange protein activated by cAMP 1 and phosphatidylinositol 3-kinase sig nals in human arterial endothelial cells. (PubMed id 21393242)^{1, 2} Wilson L.S....Maurice D.H. (2011)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Structure and regulation of the cAMP-binding domains of Epac2. (PubMed id 12469113)^{1, 2} Rehmann H....Wittinghofer A. (2003)
MAP1A light chain 2 interacts with exchange protein activated by cyclic AMP 1 (EPAC1) to enhance Rap1 GTPase activity and cell adhesion. (PubMed id 15591041)^{1, 9} Gupta M. and Yarwood S.J. (2005)
Epac increases melanoma cell migration by a heparan s ulfate-related mechanism. (PubMed id 19657062)^{1, 9} Baljinnyam E....Ishikawa Y. (2009)
Exchange protein directly activated by cAMP (EPAC) interacts with the light chain (LC) 2 of MAP1A. (PubMed id 15202935)^{1, 9} Magiera M.M....Yarwood S.J. (2004)
The cyclic AMP effector Epac integrates pro- and anti-fibrotic signals. (PubMed id 18434542)^{1, 9} Yokoyama U....Insel P.A. (2008)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 10411	HGNC: 16629	AceView: RAPGEF3	Ensembl:ENSG00000079337	euGenes: HUgn10411
ECgene: RAPGEF3	Kegg: 10411	H-InvDB: RAPGEF3

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for RAPGEF3	Pharmacogenomics, SNPs, Pathways

Intellectual Property for RAPGEF3 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for RAPGEF3 gene:
Search GeneIP for patents involving RAPGEF3

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Browse Recombinant/Natural Proteins

	OriGene Antibodies for RAPGEF3		OriGene shRNA RFP for RAPGEF3
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAPGEF3		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAPGEF3
	OriGene Protein Over-expression Lysate for RAPGEF3		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for RAPGEF3		OriGene 3'-UTR Clone for RAPGEF3
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAPGEF3		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAPGEF3
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	OriGene Purified Protein for RAPGEF3		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for RAPGEF3		OriGene Custom Protein Services for RAPGEF3
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RAPGEF3
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAPGEF3
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAPGEF3
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAPGEF3
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAPGEF3
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAPGEF3

	GenScript Custom Purified and Recombinant Proteins Services for RAPGEF3		GenScript cDNA clones with any tag delivered in your preferred vector for RAPGEF3
	GenScript Custom Assay Services for RAPGEF3		GenScript Custom Superior Antibodies Services for RAPGEF3
	GenScript Custom overexpressing Cell Line Services for RAPGEF3		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Antibodies & Assays for RAPGEF3 (Epac1)

	Regulatory tfbs in RAPGEF3 promoter
	Search Chromatin IP Primers for RAPGEF3
	RT² qPCR Primer Assay in human, mouse, rat RAPGEF3
	GNC Network for RAPGEF3
	SABiosciences Custom PCR Arrays for RAPGEF3

Tocris compounds for RAPGEF3

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

	Drugs for RAPGEF3
	Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	RAPGEF3 antibodies
	RAPGEF3 proteins
	RAPGEF3 lysates

	Antibodies for RAPGEF3
	See all of Abcam's Antibodies, Kits and Proteins for RAPGEF3
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

RAPGEF3 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for RAPGEF3

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAPGEF3

	SwitchGear 3'UTR luciferase reporter plasmids for RAPGEF3
	SwitchGear Promoter luciferase reporter plasmids for RAPGEF3

ThermoFisher Antibodies for RAPGEF3

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAPGEF3

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes RAPGEF3 gene at Home site.

hostname: 356980-web2.xennexinc.com index build: 100 solr: 1.4

Advanced Search

Export list of displayed genes

RAPGEF3 Gene

Rap guanine nucleotide exchange factor (GEF) 3

Aliases
for RAPGEF3 gene

Summaries
for RAPGEF3 gene

Genomic Views
for RAPGEF3 gene

Proteins
for RAPGEF3 gene

Protein Domains /
Families
for RAPGEF3 gene

Function
for RAPGEF3 gene

Pathways & Interactions
for RAPGEF3 gene

Drugs & Compounds
for RAPGEF3 gene

Transcripts
for RAPGEF3 gene

Expression
for RAPGEF3 gene

Orthologs
for RAPGEF3 gene

Paralogs
for RAPGEF3 gene

Genomic Variants
for RAPGEF3 gene

Disorders / Diseases
for RAPGEF3 gene

Publications
for RAPGEF3 gene

External Searches for RAPGEF3 gene

Genome Databases showing RAPGEF3 gene

Other Databases showing RAPGEF3 gene

Specialized Databases showing RAPGEF3 gene

Intellectual Property
for RAPGEF3 gene

Products
for RAPGEF3 gene

View Random Gene

Advanced Search

Export list of displayed genes

RAPGEF3 Gene

Rap guanine nucleotide exchange factor (GEF) 3

Aliasesfor RAPGEF3 gene

Summariesfor RAPGEF3 gene

Genomic Viewsfor RAPGEF3 gene

Proteinsfor RAPGEF3 gene

Protein Domains /Familiesfor RAPGEF3 gene

Functionfor RAPGEF3 gene

Pathways & Interactionsfor RAPGEF3 gene

Drugs & Compoundsfor RAPGEF3 gene

Transcriptsfor RAPGEF3 gene

Expression for RAPGEF3 gene

Orthologsfor RAPGEF3 gene

Paralogsfor RAPGEF3 gene

Genomic Variantsfor RAPGEF3 gene

Disorders / Diseasesfor RAPGEF3 gene

Publicationsfor RAPGEF3 gene

External Searches for RAPGEF3 gene

Genome Databases showing RAPGEF3 gene

Other Databases showing RAPGEF3 gene

Specialized Databases showing RAPGEF3 gene

Intellectual Propertyfor RAPGEF3 gene

Productsfor RAPGEF3 gene

View Random Gene

Aliases
for RAPGEF3 gene

Summaries
for RAPGEF3 gene

Genomic Views
for RAPGEF3 gene

Proteins
for RAPGEF3 gene

Protein Domains /
Families
for RAPGEF3 gene

Function
for RAPGEF3 gene

Pathways & Interactions
for RAPGEF3 gene

Drugs & Compounds
for RAPGEF3 gene

Transcripts
for RAPGEF3 gene

Expression
for RAPGEF3 gene

Orthologs
for RAPGEF3 gene

Paralogs
for RAPGEF3 gene

Genomic Variants
for RAPGEF3 gene

Disorders / Diseases
for RAPGEF3 gene

Publications
for RAPGEF3 gene

Intellectual Property
for RAPGEF3 gene

Products
for RAPGEF3 gene