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RAP2A Gene

protein-coding   GIFtS: 60

GC13P096884
RAP2A, member of RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: RAP2)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
K-REV 1, 2
KREV 2
OTTHUMP00000018581 2
RAP2 2
RbBP-30 2, 3
Descriptions
RAP2, member of RAS oncogene family (K-rev) 2
RAP2A, member of RAS oncogene family 2
External Ids
HGNC: 98611
Entrez Gene: 59112
UniProtKB: P101143
Ensembl: ENSG000001252497
Search outside databases for aliases for RAP2A gene

Previous GC identifers: GC13P096446 GC13P092473 GC13P096923 GC13P095784

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAP2A gene  

Entrez Gene cytogenetic band: 13q34   Ensembl cytogenetic band:  13q32.1   HGNC cytogenetic band: 13q34

RAP2A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13P096884:     (about GC identifiers)

Start:
96,884,475 bp from pter
End:
96,918,245 bp from pter
Size:
33,771 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000013.9  NT_009952.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAP2A_HUMAN, P10114 (See protein sequence)
Recommended Name: Ras-related protein Rap-2a precursor  
Size: 183 amino acids; 20615 Da
Subunit: Interacts with PLCE1. Interacts with SGSM1, SGSM2, SGSM3 and ARHGAP29. The GTP-bound form
of RAP2A interacts with RUNDC3A (By similarity)
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential)
PDB structures from and Proteopedia :
1KAO (3D)    2RAP (3D)    3RAP (3D)    
Secondary accessions: Q5JSC1 Q5JSC2

REFSEQ proteins: NP_066361.1  

ENSEMBL proteins: 
ENSP00000245304 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Rap2A)
Human Recombinant Proteins from Abnova (RAP2A)
              OriGene Purified Recombinant Human Protein: RAP2A 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005622 intracellular IEA--
GO:0005886 plasma membrane IEA--
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Antibodies for RAP2A: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (Rap2A), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (RAP2A)
Novus Biologicals Antibody for RAP2A

Assays for RAP2A: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR003577 GTPase_Ras
 IPR001806 Ras_GTPase
 IPR005225 Small_GTP_bd
 IPR013753 Ras


   GeneDecks  RAP2A for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P10114

ProtoNet protein and cluster: P10114

2 Blocks protein families:
IPB001806 Transforming protein P21 RAS signature
IPB003577 Ras small GTPase


UniProtKB/Swiss-Prot: RAP2A_HUMAN, P10114
Domain: The effector domain mediates the interaction with RUNDC3A (By similarity)
Similarity: Belongs to the small GTPase superfamily. Ras family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAP2A)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAP2A)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021033

              Applied Biosystems Silencer® siRNAs for RAP2A

              Sigma-Aldrich siRNA for RAP2A  
                     Sigma-Aldrich shRNA for RAP2A  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for RAP2A
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021033
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021033
                                 untagged cDNA clone in CMV expression vector: NM_021033 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_021033

UniProtKB/Swiss-Prot: RAP2A_HUMAN, P10114
Enzyme regulation: Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a
cAMP-dependent manner, and GFR

Genatlas biochemistry entry for RAP2A:
RAP2A,Ras oncogene superfamily member (K-rev),overexpressed in sporadic breast cancer,grade I

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003924 GTPase activity TAS1900290
GO:0005515 protein binding IPI18930710
GO:0005525 GTP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Invitrogen iPath™ Online BioAtlas - Pathways for RAP2A (Maps provided by GeneGo):
 cAMP signaling
 Rap2A regulation pathway

   GeneDecks  RAP2A for the pathways selected above  
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1 Millipore Pathway for RAP2A
 Signal transduction cAMP signaling

   GeneDecks  RAP2A for the pathways selected above  
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3 Sigma-Aldrich "Your Favorite Gene" Pathways for  RAP2A  (Your Favorite Gene powered by Ingenuity) 
 Calcium Signaling
 Integrin Signaling
 Molecular Mechanisms of Cancer

   GeneDecks  RAP2A for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  RAP2A 


5/28 Interacting proteins for RAP2A (P101141 ENSP000002453043) via UniProtKB, MINT, and/or STRING (see all 28 )
InteractantInteraction Details
GeneCardExternal ID(s)
MINK1Q8N4C81EBI-602366, EBI-2133481
RUNDC3AENSP000002254413STRING (score=.988)
TNIKENSP000002844833STRING (score=.988)
RAPGEF6ENSP000002968593STRING (score=.974)
MAP4K4ENSP000003136443STRING (score=.96)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007165 signal transduction TAS3045729
GO:0007264 small GTPase mediated signal transduction IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for RAP2A
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for RAP2A
2 Novoseek chemical compound relationships for RAP2A gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
cyclic amp 49.33 5 18391018 (1), 16452984 (1), 14506476 (1)
gtp 45.24 3 10591105 (1), 9523700 (1), 17300802 (1)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAP2A)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAP2A)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021033

              Sigma-Aldrich siRNA for RAP2A  
                     Sigma-Aldrich shRNA for RAP2A  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_021033  

REFSEQ mRNAs for RAP2A gene: 

NM_021033.6   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_021033  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021033
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021033
                                 untagged cDNA clone in CMV expression vector: NM_021033 

Additional cDNA sequence: 

AF205602.1 AF493914.1 AK090533.1 AK094586.1 AK311202.1 AK315139.1 BC022356.1 BC041333.2 
BC047495.1 BC070031.1 X12534.1 

8 DOTS entries:

DT.454556  DT.100791300  DT.120779971  DT.40113077  DT.97844836  DT.102830970  DT.95156522  DT.100791299 

24/292 AceView cDNA sequences (see all 292 ):

AI304350 F13073 BQ027312 AA767216 BF198130 BM705964 X12534 AI401270 
AI417253 T10769 AA171989 BE503099 AI288777 AI299553 T16259 AW271400 
AA488116 AI302106 AI288778 AW139422 BF198095 AI422246 AW078643 BQ007743 

highest scoring ESTs for RAP2A:

AI963476 X12534 AA007240 AA007241 AA084123 AA084155 AA122214 AA122215 AA171989 AA172305 

Unigene Cluster for RAP2A:

RAP2A, member of RAS oncogene family
Hs.508480  [show with all ESTs]
Unigene Representative Sequence: BC041333


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000245304  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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RAP2A expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RAP2A

1 / 2 / 3

11 probe-sets matching RAP2A gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
46235_at2, 3 U95-B 1 1.00 1.00 0.89 1.64 AI963476 1.00 1.00 1.00 1

41317_at2, 3 U95-A 1 1.00 1.00 0.91 0.75 X12534 1.00 1.00 1.00 1

41318_g_at2, 3 U95-A 1 1.00 1.00 0.83 0.88 X12534 1.00 1.00 1.00 1

1819_at2, 3 U95-A 1 1.00 1.00 0.48 0.40 HG1996-HT2044 0.20 0.50 0.38 1

1820_g_at2, 3 U95-A 3 1.00 0.49 0.82 0.84 HG1996-HT2044 0.20 0.50 0.38 1

225585_at2, 3 U133-B 1 1.00 1.00 -- -- AI963476 1.00 1.00 1.00 1

221830_at2, 3 U133-A 1 1.00 1.00 -- -- AI302106 0.80 1.00 0.91 1

214487_s_at2 U133-A 2 1.00 0.50 -- -- -- -- -- -- --

225585_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

221830_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

214487_s_at2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
GeneDecks  RAP2A for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AATTGTAGTT

SOURCE GeneReport for Unigene cluster: Hs.508480

Expression variation in blood from EXPOLDB for RAP2A

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RAP2A gene from 5/11 species (see all 11 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RAP2A1   -- RAP2A, member of RAS oncogene family 94.9(n)
96.47(a)
476968  XM_534168.2  XP_534168.2 
chimpanzee
(Pan troglodytes)
RAP2A1   -- RAP2A, member of RAS oncogene family 99.82(n)
99.45(a)
452629  XM_509705.2  XP_509705.2 
cow
(Bos taurus)
RAP2A1   -- RAP2A, member of RAS oncogene family 93.26(n)
99.45(a)
522707  XM_863890.2  XP_868983.1 
mouse
(Mus musculus)
Rap2a1, 5 14 (59.00 cM)5
RAS related protein 2a1, 5 94.35(n)1
99.45(a)1
761081  NM_029519.31  NP_083795.21 
 AI4514095  AI8472125  (see all 15)
chicken
(Gallus gallus)
RAP2A1   -- RAP2A, member of RAS oncogene family 89.62(n)
98.36(a)
769797  XM_001233103.1  XP_001233104.1 
About this table        Species with no ortholog for RAP2A

ENSEMBL Gene Tree for RAP2A
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for RAP2A gene
RAP2C2  RAP2B2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/141 NCBI SNPs in RAP2A are shown (see all 141 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 53)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 13 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs23899101,2
A,C,F,H,O96915165(+) AACCAA/GTGTAT 1 -- ut31 ese314Minor allele frequency- N:0.00EU EA WA NA 1232
rs79956501,2
A,C,F,H96918646(+) GGATCC/TGAAGG 1 -- ng317Minor allele frequency- T:0.03EU EA WA NA 560
rs128739191,2
C,F,H96917525(+) ACTTAC/TACAAA 1 -- ut3112Minor allele frequency- N:0.00EU EA WA NA 1054
rs116178871,2
H96918573(+) AGCAAT/CATCAC 1 -- ng314Minor allele frequency- C:0.00EU EA WA 418
rs23899091,2
A,C,F,H96895025(+) AGGAAG/TTTTTT 1 -- int16Minor allele frequency- T:0.46EA WA NA 440
rs79970791,2
A,C,F,H96910237(+) AGCTAT/CTTATG 1 -- int17Minor allele frequency- C:0.00EU EA WA NA 552
--
rs95132441,2
A,C,F,H96903833(+) gctgaG/Atagtg 1 -- int14Minor allele frequency- A:0.15EU EA WA 418
rs95543961,2
A,C,F,H96913193(+) ATCCAG/CTTTCC 1 -- int14Minor allele frequency- C:0.36EU EA WA 420
rs13043921,2
A,C,F,H96889009(-) ACACAA/GATAAT 1 -- int17Minor allele frequency- G:0.39NA EA EU WA 666
rs20390861,2
A,C,F,H96896525(+) TCTCTT/CTTTTT 1 -- int1 trp34Minor allele frequency- C:0.15EU EA WA 418
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HapMap Linkage Disequilibrium images for RAP2A (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 179540

Tumor Gene Database: RAP2A

(Possibly Related Articles in Doctor's Guide)
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  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/55 PubMed articles for RAP2A gene (see all 55 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 5911 HGNC: 9861 AceView: RAP2A Ensembl:ENSG00000125249 euGenes: HUgn5911
ECgene: RAP2A H-InvDB: RAP2A
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for RAP2A Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RAP2A:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for RAP2A Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for RAP2A Browse small molecules at Sigma-Aldrich
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for RAP2A




 Drugs for RAP2A
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for RAP2A

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