RAF1 Gene
protein-coding GIFtS: 76
GCID: GC03M012625
|
|
v-raf-1 murine leukemia viral oncogene homolog 1
| |
Aliases
for RAF1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| V-Raf-1 Murine Leukemia Viral Oncogene Homolog 11 2 | | Oncogene RAF12 | | Raf-11 2 3 | | Raf Proto-Oncogene Serine/Threonine Protein Kinase2 | | CRAF1 2 5 | | RAF Proto-Oncogene Serine/Threonine-Protein Kinase2 | | C-Raf1 | | RAF3 | | Proto-Oncogene C-RAF2 3 | | CRaf1 | | EC 2.7.11.13 8 | | EC 2.7.118 | | NS52 5 | | |
Export aliases for RAF1 gene to outside databasesPrevious GC identifers: GC03M012554 GC03M012600 GC03M012559 |
Summaries
for RAF1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for RAF1:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K),which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Onceactivated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2,which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKsare pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved inthe cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associatedwith Noonan syndrome 5 and LEOPARD syndrome 2. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPasesand the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisionsincluding proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiatesa mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specificMAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2).The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of celldeath at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation.Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscletroponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis(MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also bytranslocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rhosignaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation ofepithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptionalrepressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selectedstimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation
 summary
for RAF1: Raf kinases, a family of three serine/threonine kinases, are part of the ras-MAPK signaling cascade and
phosphorylate MEK. Upon growth factor stimulation, Raf-1 (or c-Raf) is activated by GTP-bound Ras and
recruited to the cell membrane. This activation process is tightly regulated by a number of factors
including phosphatases (e.g. PP1, PP2A, PP5), kinases (e.g. Src, ERK, Akt, PKC) and proteins that bind
directly to Raf-1 (e.g. RKIP, 14-3-3zeta, KSR, Hsp90). Raf-1 is also thought to be able to dimerize with
wild type B-Raf in a Ras-dependent process. B-raf is commonly mutated and thereby activated in many human
cancers, the most frequent mutation being the V600E mutation of the kinase domain. Whilst wt b-Raf and Raf-1
are strongly activated by growth factor signals via Ras and Src, a-Raf is only modestly activated and has
low basal activity. All three isoforms of Raf are considered to be oncogenic.
Gene Wiki entry for RAF1 (C-Raf)
|
Genomic Views
for RAF1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000003.11 NC_018914.1 NT_022517.18
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the RAF1 gene promoter:
TBP STAT1 AP-1 ATF-2 STAT5A CREB deltaCREB STAT3 c-Jun
Other transcription factors
Search SABiosciences Chromatin IP Primers for RAF1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAF1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 3p25 Ensembl cytogenetic band: 3p25.2 HGNC cytogenetic band: 3p25RAF1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03M012625: view genomic region
(about GC identifiers)
Start:
|
12,625,100 bp from pter |
End:
|
12,705,725 bp from pter |
Size:
|
80,626 bases |
Orientation:
|
minus strand |
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Proteins
for RAF1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049 (See
protein sequence)Recommended Name: RAF proto-oncogene serine/threonine-protein kinase Size: 648 amino acids; 73052 Da
Cofactor: Binds 2 zinc ions per subunit
Subunit: Monomer. Homodimer. Heterodimerizes with BRAF and this heterodimer possesses a highly increased kinaseactivity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of theheterodimer. Forms a multiprotein complex with Ras (M-Ras/MRAS), SHOC2 and protein phosphatase 1 (PPP1CA, PPP1CB andPPP1CC). Interacts with Ras proteins; the interaction is antagonized by RIN1. Weakly interacts with RIT1. Interacts(via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with BRAF,a ternary complex inhibited by GNAI1 (By similarity). Interacts with STK3/MST2; the interaction inhibits itspro-apoptotic activity. Interacts (when phosphorylated at Ser-259) with YWHAZ (unphosphorylated at 'Thr-232').Interacts with MAP2K1/MEK1 and MAP2K2/MEK2 (By similarity). Interacts with MAP3K5/ASF1 (via N-terminus) and thisinteraction inhibits the proapoptotic function of MAP3K5/ASK1. Interacts with PAK1 (via kinase domain). Thephosphorylated form interacts with PIN1. The Ser-338 and Ser-339 phosphorylated form (by PAK1) interacts with BCL2.Interacts with PEBP1/RKIP and this interaction is enhanced if RAF1 is phosphorylated on residues Ser-338, Ser-339,Tyr-340 and Tyr-341. Interacts with ADCY2, ADCY5, ADCY6, DGKH, RCAN1/DSCR1, ROCK2, PPP1R12A, PKB/AKT1, PPP2CA,PPP2R1B, SPRY2, SPRY4, CNKSR1/CNK1, KSR2 and PHB/prohibitin. In its active form, interacts with PRMT5
Subcellular location: Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in boththe cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cellmembrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondriallocalization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus
6/15 PDB 3D structures from  and Proteopedia  for RAF1 (see all 15):1C1Y (3D)
1FAQ (3D)
1FAR (3D)
1GUA (3D)
1RFA (3D)
3CU8 (3D)
Secondary accessions: B0LPH8 B2R5N3 Q15278 Q9UC20Alternative splicing: 2 isoforms: P04049-1 P04049-2 Explore the universe of human proteins at neXtProt for RAF1: NX_P04049
Post-translational modifications:
Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29,Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation at Ser-259induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complexcontaining protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.Phosphorylation at Ser-338 by PAK1 and PAK7/PAK5 and Ser-339 by PAK1 is required for its mitochondrial localization.Phosphorylation at Ser-621 in response to growth factor treatment stabilizes the protein, possibly by preventingproteasomal degradation. Phosphorylation at Ser-289, Ser-296, Ser-301, Ser-338 and Ser-621 are somehow linked to themethylation potential of cells. Treatment of cells with HGF in the presence of the methylation inhibitor5'-methylthioadenosine (MTA) results in increased phosphorylation at Ser-338 and Ser-621 and decreased phosphorylationat Ser-296, Ser-301 and Ser-3381
Methylated at Arg-563 in response to EGF treatment. This modification leads to destabilization of the protein, possiblythrough proteasomal degradation1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P04049
4/32 DME Specific Peptides for RAF1 (P04049) (see all 32)
RAF1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_002871.1
ENSEMBL proteins: ENSP00000251849 ENSP00000401888 ENSP00000398591 ENSP00000401088 ENSP00000391265 ENSP00000443567 ENSP00000441186 Reactome Protein details: P04049
Human Recombinant Protein Products:
Gene Ontology (GO): 5/8 cellular component terms (GO ID links to tree view) (see all 8): About this table
RAF1 for ontologies About GeneDecksing
RAF1 Antibody Products:
Assay Products for RAF1: |
Protein
Domains /
Families
for RAF1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
RAF1 for domains About GeneDecksing
5/8 InterPro domains/families (see all 8):Graphical View of Domain Structure for InterPro Entry P04049ProtoNet protein and cluster: P04049 2 Blocks protein families: IPB002219 Protein kinase C IPB003116 Raf-like Ras-binding
UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamilySimilarity: Contains 1 phorbol-ester/DAG-type zinc fingerSimilarity: Contains 1 protein kinase domainSimilarity: Contains 1 RBD (Ras-binding) domain |
Function
for RAF1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPasesand the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisionsincluding proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiatesa mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specificMAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2).The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of celldeath at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation.Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscletroponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis(MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also bytranslocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rhosignaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation ofepithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptionalrepressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selectedstimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiationCatalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme regulation: Regulation is a highly complex process involving membrane recruitment, protein-protein interactions,dimerization, and phosphorylation/dephosphorylation events. Ras-GTP recruits RAF1 to the membrane, thereby promotingits activation. The inactive conformation of RAF1 is maintained by autoinhibitory interactions occurring between theN-terminal regulatory and the C-terminal catalytic domains and by the binding of a 14-3-3 protein that contacts twophosphorylation sites, Ser-259 and Ser-621. Upon mitogenic stimulation, Ras and PPP2R1A cooperate to releaseautoinhibition and the subsequent phosphorylation of activating sites: Ser-338, Tyr-341, Thr-491, and Ser-494, yieldsa fully active kinase. Through a negative feedback mechanism involving MAPK1/ERK2, RAF1 is phosphorylated on Ser-29,Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2, which yields an inactive, desensitized kinase. Thesignaling-competent conformation of RAF1 is finally re-established by the coordinated action of PIN1, a prolylisomerase that converts pSer and pThr residues from the cis to the trans conformation, which is preferentiallyrecognized and dephosphorylated by PPP2R1A. Activated by homodimerization and heterodimerization (with BRAF). Alsoregulated through association with other proteins such as KSR2, CNKSR1/CNK1, PEBP1/RKIP, PHB/prohibitin and SPRY4.PEBP1/RKIP acts by dissociating RAF1 from its substrates MAP2K1/MEK1 and MAP2K2/MEK2. PHB/prohibitin facilitates thedisplacement of 14-3-3 from RAF1 by activated Ras, thereby promoting cell membrane localization and phosphorylation ofRAF1 at the activating Ser-338. SPRY4 inhibits Ras-independent, but not Ras-dependent, activation of RAF1. CNKSR1/CNK1regulates Src-mediated RAF1 activation Genatlas biochemistry entry for RAF1:murine sarcoma viral (v-raf-1) oncogene homolog 1 (mitogenic factor) interacting with TC21 for activation andtranslocation to plasma membrane
Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAF1 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAF1 (see all 5)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: RAF1 (NM_002880) | |  | Sino Biological Human cDNA Clone for RAF1 | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RAF1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAF1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAF1 |
Gene Ontology (GO): 5/11 molecular function terms (GO ID links to tree view) (see all 11): About this table
RAF1 for ontologies About GeneDecksing
4 GenomeRNAi human phenotypes for RAF1:
Animal Models:
Mouse knock-outs for RAF1: Raf1tm1Zim Raf1tm2.1Bacc Raf1tm1Ulb Raf1tm1Bacc
15/18 MGI mutant phenotypes (inferred from 10 alleles ) (MGI details for Raf1) (see all 18):
RAF1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for RAF1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/167 super-pathways (see all 167) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Development_CNTF receptor signaling | | | 2 | Development HGF signaling pathway | | | 3 | IL-9 Signaling Pathways | | | 4 | Signaling by FGFR | | | 5 | ErbB4 Pathway | |
Pathway sources
See GeneCards unified pathways
Show all pathways
5/86 EMD Millipore Pathways for RAF1 (see all 86)
5/9  R&D Systems Pathways for RAF1 (see all 9)
5/99 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for RAF1 (see all 99)
1 
Cell Signaling Technology (CST) Pathway for RAF1
3 Tocris Bioscience Pathways for RAF1
5/89  GeneGo (Thomson Reuters) Pathways for RAF1 (see all 89)
5/55  BioSystems Pathways for RAF1 (see all 55)
5/67 
Reactome Pathways for RAF1 (see all 67)
4  PharmGKB Pathways for RAF1
5/35 
Kegg Pathways (Kegg details for RAF1) (see all 35):
RAF1 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for RAF1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/201 Interacting proteins for RAF1 (P040491, 2, 3 ENSP000002518494) via UniProtKB, MINT, STRING, and/or I2D (see all 201)| Interactant | Interaction Details |
|---|
| GeneCard | External ID(s) |
|---|
| YWHAZ | P631041, 2, 3, ENSP000003095034 | EBI-365996,EBI-347088 MINT-8004922 MINT-8005899 MINT-8010094 MINT-8009701 MINT-45799 MINT-19373 MINT-8006572 MINT-8004938 MINT-8006579 MINT-19372 MINT-8004992 MINT-8004961 MINT-8008718 MINT-45800 I2D:
score=7 STRING: ENSP00000309503 | | HRAS | P011121, 2, 3, ENSP000003098454 | EBI-365996,EBI-350145 MINT-6540149 MINT-60984 MINT-7295101 MINT-6802882 MINT-18357 MINT-17120 MINT-6946517 MINT-6540170 MINT-17119 I2D:
score=10 STRING: ENSP00000309845 | | BRAF | P150561, 2, 3, ENSP000002886024 | EBI-365996,EBI-365980 MINT-8396824 MINT-8396759 MINT-8396808 MINT-8396838 MINT-8397040 I2D:
score=7 STRING: ENSP00000288602 | | YWHAB | P319461, 2, 3, ENSP000003001614 | EBI-365996,EBI-359815 MINT-8004910 MINT-8004954 MINT-8005906 MINT-8004894 MINT-8009103 I2D:
score=5 STRING: ENSP00000300161 | | RAP1A | P628341, 2, 3, ENSP000003487864 | EBI-365996,EBI-1047696 MINT-24923 I2D:
score=6 STRING: ENSP00000348786 |
About this table
Gene Ontology (GO): 5/31 biological process terms (GO ID links to tree view) (see all 31): About this table
RAF1 for ontologies About GeneDecksing
|
Drugs & Compounds
for RAF1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
RAF1 for compounds About GeneDecksing
 |
Enzo Life Sciences drugs & compounds for RAF1 |
Compounds for RAF1 available from Tocris Bioscience About this table
| Compound | Action |
CAS
# |
|---|
| ZM 336372 | Potent, selective c-Raf inhibitor | [208260-29-1] | | GW 5074 | Potent, selective c-Raf1 kinase inhibitor | [220904-83-6] | | L-779,450 | Potent Raf kinase inhibitor | [303727-31-3] |
10/845 HMDB Compounds for RAF1 (see all 845) About this table
1 DrugBank Compound for RAF1 About this table
10/127 Novoseek chemical compound relationships for RAF1 gene (see all 127) About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| gw-5074 |
88.1 |
20 |
15255937 (3), 16440327 (2), 19288477 (2), 16546979 (1) (see all 14) |
| zm 336372 |
80.8 |
15 |
18063082 (3), 16603190 (2), 15956248 (2), 16909118 (1) (see all 5) |
| threonine |
80.2 |
136 |
9689060 (3), 7803760 (3), 7685905 (2), 1461284 (2) (see all 99) |
| tyrosine |
77.2 |
270 |
8622647 (5), 8662746 (5), 7692235 (5), 7542586 (4) (see all 99) |
| pd 98,059 |
76.2 |
26 |
12389631 (2), 10727406 (1), 11703098 (1), 14736735 (1) (see all 20) |
| geldanamycin |
74.5 |
41 |
10927059 (4), 9137531 (4), 12471618 (3), 15846067 (2) (see all 19) |
| 17-(allylamino)-17-demethoxygeldanamycin |
73.3 |
8 |
9622079 (1), 11280726 (1), 14506167 (1), 18281495 (1) (see all 5) |
| serine |
72.9 |
163 |
7623807 (3), 1645717 (3), 7685905 (2), 9234708 (2) (see all 99) |
| sorafenib |
72.2 |
53 |
19710032 (3), 16890795 (2), 16503817 (1), 17071608 (1) (see all 37) |
| radicicol |
72.1 |
11 |
10979978 (2), 18676857 (1), 10945979 (1), 11306353 (1) (see all 8) |
Search CenterWatch for drugs/clinical trials and news about RAF1
|
Transcripts
for RAF1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for RAF1 gene: NM_002880.3 Unigene Cluster for RAF1: V-raf-1 murine leukemia viral oncogene homolog 1 Hs.159130 [show with all ESTs]Unigene Representative Sequence: GU14930214 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000251849(uc011aut.2 uc003bxf.4 uc011auu.2) ENST00000442415
ENST00000432427 ENST00000423275 ENST00000471449 ENST00000465826 ENST00000475353
ENST00000494557 ENST00000460610 ENST00000491290 ENST00000416093 ENST00000492690
ENST00000542177 ENST00000534997
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAF1 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAF1 (see all 5)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: RAF1 (NM_002880) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for RAF1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAF1 |
Additional cDNA sequence: AK055589.1 AK124449.1 AK226028.1 AK303561.1 AK303920.1 AK312248.1 BC018119.2 BX648063.1 FM209427.1 GU149302.1 X03484.1 20 DOTS entries: DT.100669838 DT.100669840 DT.100669848 DT.87008986 DT.91838878 DT.75151772 DT.95278225 DT.95278214 DT.100669835 DT.120918106 DT.91705198 DT.91993563 DT.92451812 DT.100690754 DT.120918099 DT.120918136 DT.91695943 DT.40190406 DT.91826781 DT.95337142 24/551 AceView cDNA sequences (see all 551): H92852 BU633125 BI861570 BM696301 AI355606 BQ681059 AA331329 BQ945040 CB143673 BM128626 BM766547 BX506853 CR617502 AI865703 BP374718 BU193159 CN483290 BQ682769 BM766331 BQ431831 AI923038 BI821438 BQ925255 BQ680785
GeneLoc Exon Structure
|
Expression
for RAF1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| RAF1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TGTATACAAG
About this image
See RAF1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for RAF1
SOURCE GeneReport for Unigene cluster: Hs.159130
UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049Tissue specificity: In skeletal muscle, isoform 1 is more abundant than isoform 2
SABiosciences Expression via Pathway-Focused PCR Arrays including RAF1 (see all 12):
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAF1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RAF1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAF1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAF1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAF1 |
Orthologs
for RAF1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for RAF1 gene from 8/26 species (see all 26) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
RAF11 |
v-raf-1 murine leukemia viral oncogene homolog 1 |
81.5(n)
94.74(a) |
|
396245 NM_205307.1 NP_990638.1 |
lizard
(Anolis carolinensis) |
Reptilia |
RAF16 |
-- |
92(a) |
1 ↔ 1 |
2(158967363-158996331) |
African clawed frog
(Xenopus laevis) |
Amphibia |
c-raf2 |
raf protein |
76.42(n) |
|
S74063.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
craf2 |
murine leukemia viral oncogene homolog |
74.13(n) |
|
30716 BC054662.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
phl3 |
dorsal/ventral axis determination,
follicular more |
47(a) |
|
1 3A1 -- |
worm
(Caenorhabditis elegans) |
Secernentea |
lin-453 |
Expression: head neurons, neurons |
54(a) |
|
IV(6743426-6747592) -- |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
AT1G047006AT5G576106(see all 7) |
PB1 domain-containing protein tyrosine kinaseocticosapeptide/Phox/Bem1p domain-containing prote...(see all 7) |
9(a)9(a)(see all 7) |
many ↔ manymany ↔ many(see all 7) |
1(1316254-1320919) 5(23324791-23329347) |
rice
(Oryza sativa) |
Liliopsida |
----(see all 5) |
protein kinase domain containing protein, expresse...protein kinase domain containing protein, expresse...(see all 5) |
9(a)8(a)(see all 5) |
many ↔ manymany ↔ many(see all 5) |
6(26398279-26404234) 7(14722116-14730584) |
ENSEMBL Gene Tree for RAF1 (if available)
TreeFam Gene Tree for RAF1 (if available) |
Paralogs
for RAF1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for RAF1 gene
- MAP3K112 MAP3K122 KIAA18042 KSR22 KSR12 MOS2 BRAF2 MAP3K102
- MAP3K92 ARAF2 MAP3K72 MAP3K7CL2 TNNI3K2 ZAK2 MAP3K132 ILK2
17 SIMAP similar genes for RAF1 using alignment to 5 protein entries: RAF1_HUMAN (see all proteins):BRAF ARAF NUAK1 CDK4 STK11 STK36
TXK STK25 DKFZp564L2416 FRK RPS6KA2 ZAK
MAP4K3 FGR PAK2 FES SRGAP3
RAF1 for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for RAF1
PGOHUM00000245458
|
Genomic Variants
for RAF1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 3 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for RAF1 (12625100 - 12705725 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 7 variations for RAF1
4 CNVs: 53782 29726 63398 8414
2 Indels: 79681 91199
1 Inversion: 37239
Human Gene Mutation Database (HGMD): RAF1
| SABiosciences Cancer Mutation PCR Assays |
| 2 SABiosciences qBiomarker Copy Number PCR Arrays containing RAF1: |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAF1 |
|
Disorders
/ Diseases
for RAF1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
RAF1 for disorders About GeneDecksing
OMIM gene information: 164760
OMIM disorders: 611553 611554
UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disordercharacterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay,and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimatedincidence of 1 in 1000-2500 live births Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is anautosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines,electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia,retardation of growth, and deafness
20/130  diseases for RAF1 (see all 130): About MalaCardsnoonan syndrome 5 leopard syndrome 2 leopard syndrome noonan syndrome
st. louis encephalitis von hippel-lindau disease tick-borne encephalitis murray valley encephalitis
leukemia west nile fever non-small cell lung carcinoma developmental disabilities
laryngeal squamous cell carcinoma pulmonic stenosis clear cell renal cell carcinoma primitive neuroectodermal tumor
non-hodgkin lymphoma squamous cell carcinoma soft tissue sarcoma juvenile myelomonocytic leukemia
10 diseases from the University of Copenhagen DISEASES database for RAF1:Yellow fever Hepatitis tick-borne encephalitis Hepatitis C
Dengue disease Murray Valley encephalitis St. Louis encephalitis West Nile fever
Kyasanur forest disease Encephalitis 10/85 Novoseek disease relationships for RAF1 gene (see all 85) About this table
| Disease |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| noonan syndrome |
58.6 |
7 |
19953625 (2), 17603483 (2), 20052757 (1), 20461756 (1) (see all 5) |
| cancer |
55.9 |
83 |
12781369 (3), 19715444 (2), 18221149 (2), 17555829 (2) (see all 67) |
| tumors |
53 |
192 |
16926634 (6), 8451761 (3), 15803144 (3), 10830717 (3) (see all 99) |
| viral leukemia |
46.5 |
3 |
1783381 (1), 15845465 (1), 10449917 (1) |
| shock |
45.8 |
28 |
9622079 (1), 11280726 (1), 14724288 (1), 15338192 (1) (see all 26) |
| melanoma |
38.2 |
31 |
19737955 (8), 17018604 (3), 17699719 (2), 14654778 (2) (see all 15) |
| thyroid carcinoma medullary |
36.1 |
8 |
16926634 (4), 17363508 (2), 15572361 (1), 9690518 (1) |
| leukemia |
34.6 |
22 |
16250879 (3), 12903397 (3), 9632143 (2), 9242431 (1) (see all 14) |
| solid tumor |
34 |
5 |
15585643 (1), 20178713 (1), 16498671 (1), 16890795 (1) |
| breast cancer |
33.2 |
30 |
7834453 (4), 12432273 (2), 16613992 (2), 11796827 (2) (see all 20) |
GeneTests: RAF1
LEOPARD SyndromeNoonan Syndrome
Human Genome Epidemiology (HuGE) Navigator: RAF1 (14 documents)
Tumor Gene Database (TGDB): RAF1
Export disorders for RAF1 gene to outside databases
|
Publications
for RAF1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for RAF1 gene, integrated from 9 sources (see all 1554):
(articles sorted by number of sources associating them with RAF1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1. (PubMed id 11733498)1, 2, 9 Zang M....Luo Z. (2002)
- Retinoic acid induces nuclear accumulation of Raf1 du ring differentiation of HL-60 cells. (PubMed id 19298812)1, 2, 9 Smith J....Yen A. (2009)
- p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association. (PubMed id 15849194)1, 2, 9 Jin S....Field J. (2005)
- Raf-1 promotes cell survival by antagonizing apoptosis signal-regulating kinase 1 through a MEK-ERK independent mechanism. (PubMed id 11427728)1, 2, 9 Chen J....Fu H. (2001)
- Diacylglycerol kinase eta augments C-Raf activity and B-Raf/C-Raf heterodimerization. (PubMed id 19710016)1, 2, 9 Yasuda S....Sakane F. (2009)
- Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding. (PubMed id 16093354)1, 2, 9 Zhu J....Tzivion G. (2005)
- Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1. (PubMed id 12717443)1, 2, 9 Sasaki A....Yoshimura A. (2003)
- The solution structure of the Raf-1 cysteine-rich domain: a novel ras and phospholipid binding site. (PubMed id 8710867)1, 2, 9 Mott H.R....Campbell S.L. (1996)
- Phosphorylation of Raf by ceramide-activated protein kinase. (PubMed id 7477354)1, 2, 9 Yao B.... Kolesnick R. (1995)
- Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. (PubMed id 17603483)1, 2, 9 Pandit B.... Gelb B.D. (2007)
|
External Searches for RAF1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing RAF1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing RAF1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RAF1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for RAF1 | Pharmacogenomics, SNPs, Pathways | | ATLAS Chromosomes in Cancer entry for RAF1 | Genetics and Cytogenetics in Oncology and Haematology | | GeneReviews | http://www.ncbi.nlm.nih.gov/sites/GeneTests/lab/gene/RAF1 | | NIEHS-SNPs | http://egp.gs.washington.edu/data/raf1/ |
|
| | |
About This Section
| Patent Information for RAF1 gene:
Search GeneIP for patents involving RAF1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for RAF1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for RAF1 | | OriGene shRNA RFP for RAF1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAF1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAF1 | | | OriGene Protein Over-expression Lysate for RAF1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for RAF1 | | OriGene 3'-UTR Clone for RAF1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAF1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAF1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for RAF1 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for RAF1 | | OriGene Custom Protein Services for RAF1 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RAF1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAF1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAF1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAF1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAF1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAF1 |
| |
 |  |  |  | |
| | Antibodies & Assays for RAF1 |
| | | | Tocris compounds for RAF1 |
| |  |  |  |  | | | | |
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 |
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 | | | RAF1 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAF1 |
|  |  |  | | | | ThermoFisher Antibody for RAF1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAF1 |
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