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RAF1 Gene

protein-coding   GIFtS: 76
GCID: GC03M012625

V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1

  See RAF1-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 11 2     Oncogene RAF12
Proto-Oncogene C-RAF2 3     Raf Proto-Oncogene Serine/Threonine Protein Kinase2
Raf-12 3     RAF Proto-Oncogene Serine/Threonine-Protein Kinase2
EC 2.7.11.13 8     RAF3
CRAF2 5     cRaf3
NS52 5     EC 2.7.118
c-Raf2     

External Ids:    HGNC: 98291   Entrez Gene: 58942   Ensembl: ENSG000001321557   OMIM: 1647605   UniProtKB: P040493   

Export aliases for RAF1 gene to outside databases

Previous GC identifers: GC03M012554 GC03M012600 GC03M012559


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for RAF1 Gene:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase
(MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly.
Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1
and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.
Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene
expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in
this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. (provided by RefSeq, Jul 2008)

GeneCards Summary for RAF1 Gene:
RAF1 (v-raf-1 murine leukemia viral oncogene homolog 1) is a protein-coding gene. Diseases associated with RAF1 include raf1-related leopard syndrome, and noonan syndrome 5. GO annotations related to this gene include protein serine/threonine kinase activity and identical protein binding. An important paralog of this gene is FPGT-TNNI3K.

UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049
Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras
GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate
decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1
activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential
phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular
signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and
Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases:
ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the
phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit
signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and
angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds
BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for
normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ
protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces
down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation,
pathogen-mediated macrophage apoptosis, and erythroid differentiation

summary for RAF1 Gene:
Raf kinases, a family of three serine/threonine kinases, are part of the ras-MAPK signaling cascade and
phosphorylate MEK. Upon growth factor stimulation, Raf-1 (or c-Raf) is activated by GTP-bound Ras and
recruited to the cell membrane. This activation process is tightly regulated by a number of factors
including phosphatases (e.g. PP1, PP2A, PP5), kinases (e.g. Src, ERK, Akt, PKC) and proteins that bind
directly to Raf-1 (e.g. RKIP, 14-3-3zeta, KSR, Hsp90). Raf-1 is also thought to be able to dimerize with
wild type B-Raf in a Ras-dependent process. B-raf is commonly mutated and thereby activated in many human
cancers, the most frequent mutation being the V600E mutation of the kinase domain. Whilst wt b-Raf and Raf-1
are strongly activated by growth factor signals via Ras and Src, a-Raf is only modestly activated and has
low basal activity. All three isoforms of Raf are considered to be oncogenic.

Gene Wiki entry for RAF1 (C-Raf) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000003.11  NT_022517.19  NC_018914.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the RAF1 gene promoter:
         TBP   STAT1   AP-1   ATF-2   STAT5A   CREB   deltaCREB   STAT3   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): RAF1 promoter sequence
   Search Chromatin IP Primers for RAF1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat RAF1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p25   Ensembl cytogenetic band:  3p25.2   HGNC cytogenetic band: 3p25

RAF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RAF1 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M012625:  view genomic region     (about GC identifiers)

Start:
12,625,100 bp from pter      End:
12,705,725 bp from pter
Size:
80,626 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049 (See protein sequence)
Recommended Name: RAF proto-oncogene serine/threonine-protein kinase  
Size: 648 amino acids; 73052 Da
Cofactor: Binds 2 zinc ions per subunit
Subunit: Monomer. Homodimer. Heterodimerizes with BRAF and this heterodimer possesses a highly increased kinase
activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced
by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the
heterodimer. Forms a multiprotein complex with Ras (M-Ras/MRAS), SHOC2 and protein phosphatase 1 (PPP1CA, PPP1CB
and PPP1CC). Interacts with Ras proteins; the interaction is antagonized by RIN1. Weakly interacts with RIT1.
Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary
complex with BRAF, a ternary complex inhibited by GNAI1 (By similarity). Interacts with STK3/MST2; the
interaction inhibits its pro-apoptotic activity. Interacts (when phosphorylated at Ser-259) with YWHAZ
(unphosphorylated at 'Thr-232'). Interacts with MAP2K1/MEK1 and MAP2K2/MEK2 (By similarity). Interacts with
MAP3K5/ASF1 (via N-terminus) and this interaction inhibits the proapoptotic function of MAP3K5/ASK1. Interacts
with PAK1 (via kinase domain). The phosphorylated form interacts with PIN1. The Ser-338 and Ser-339
phosphorylated form (by PAK1) interacts with BCL2. Interacts with PEBP1/RKIP and this interaction is enhanced if
RAF1 is phosphorylated on residues Ser-338, Ser-339, Tyr-340 and Tyr-341. Interacts with ADCY2, ADCY5, ADCY6,
DGKH, RCAN1/DSCR1, ROCK2, PPP1R12A, PKB/AKT1, PPP2CA, PPP2R1B, SPRY2, SPRY4, CNKSR1/CNK1, KSR2 and
PHB/prohibitin. In its active form, interacts with PRMT5
Selected PDB 3D structures from and Proteopedia for RAF1 (see all 19):
1C1Y (3D)        1FAQ (3D)        1FAR (3D)        1GUA (3D)        1RFA (3D)        3CU8 (3D)    
Secondary accessions: B0LPH8 B2R5N3 Q15278 Q9UC20
Alternative splicing: 2 isoforms:  P04049-1   P04049-2   

Explore the universe of human proteins at neXtProt for RAF1: NX_P04049

Explore proteomics data for RAF1 at MOPED

Post-translational modifications: 

  • Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at
    Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation
    at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by
    the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate
    RAF1 activity. Phosphorylation at Ser-338 by PAK1 and PAK7/PAK5 and Ser-339 by PAK1 is required for its
    mitochondrial localization. Phosphorylation at Ser-621 in response to growth factor treatment stabilizes the
    protein, possibly by preventing proteasomal degradation. Phosphorylation at Ser-289, Ser-296, Ser-301, Ser-338
    and Ser-621 are somehow linked to the methylation potential of cells. Treatment of cells with HGF in the presence
    of the methylation inhibitor 5'-methylthioadenosine (MTA) results in increased phosphorylation at Ser-338 and
    Ser-621 and decreased phosphorylation at Ser-296, Ser-301 and Ser-338. Dephosphorylation at Ser-338 by PPP5C
    results in a activity decrease1
  • Methylated at Arg-563 in response to EGF treatment. This modification leads to destabilization of the protein,
    possibly through proteasomal degradation1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for RAF1 (P04049) (see all 32)
     NILLFMG  GSVLWMA  SNGFGFK  WMAPEVI 


    See RAF1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_002871.1  
    ENSEMBL proteins: 
     ENSP00000251849   ENSP00000401888   ENSP00000398591   ENSP00000401088   ENSP00000391265  
     ENSP00000443567   ENSP00000441186  
    Reactome Protein details: P04049

    RAF1 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant RAF1 Protein
    R&D Systems Recombinant & Natural Proteins for RAF1 (Raf-1)
    Enzo Life Sciences proteins for RAF1
    OriGene Purified Proteins for RAF1
    OriGene Protein Over-expression Lysate for RAF1
    OriGene MassSpec for RAF1
    OriGene Custom Protein Services for RAF1
    GenScript Custom Purified and Recombinant Proteins Services for RAF1
    Novus Biologicals RAF1 Proteins
    Novus Biologicals RAF1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for RAF1 

     
    antibodies-online proteins for RAF1 (19 products) 

     
    antibodies-online peptides for RAF1

    RAF1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of RAF1
    R&D Systems Antibodies for RAF1 (Raf-1)
    Cell Signaling Technology (CST) Antibodies for RAF1 
    OriGene Antibodies for RAF1
    OriGene Custom Antibody Services for RAF1
    Novus Biologicals RAF1 Antibodies
    Abcam antibodies for RAF1
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibody for RAF1
    antibodies-online antibodies for RAF1 (646 products) 

    RAF1 Assay Products:

    EMD Millipore Kits and Assays for the Analysis of RAF1
    OriGene Custom Assay Services for RAF1
    Browse R&D Systems for biochemical assays
    GenScript RAF1-Activity-based Kinase Assay for Compound Screening
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for RAF1 
    antibodies-online kits for RAF1 (20 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: v-raf-1 murine leukemia viral oncogene homolog 1
    RAF family

    Selected InterPro protein domains (see all 8):
     IPR017441 Protein_kinase_ATP_BS
     IPR020454 DAG/PE-bd
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry P04049

    ProtoNet protein and cluster: P04049

    2 Blocks protein domains:
    IPB002219 Protein kinase C
    IPB003116 Raf-like Ras-binding


    UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049
    Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily
    Similarity: Contains 1 phorbol-ester/DAG-type zinc finger
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 RBD (Ras-binding) domain


    Find genes that share domains with RAF1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: RAF1_HUMAN, P04049
    Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras
    GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate
    decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1
    activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential
    phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular
    signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and
    Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases:
    ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the
    phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit
    signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and
    angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds
    BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for
    normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ
    protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces
    down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation,
    pathogen-mediated macrophage apoptosis, and erythroid differentiation
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Regulation is a highly complex process involving membrane recruitment, protein-protein
    interactions, dimerization, and phosphorylation/dephosphorylation events. Ras-GTP recruits RAF1 to the membrane,
    thereby promoting its activation. The inactive conformation of RAF1 is maintained by autoinhibitory interactions
    occurring between the N-terminal regulatory and the C-terminal catalytic domains and by the binding of a 14-3-3
    protein that contacts two phosphorylation sites, Ser-259 and Ser-621. Upon mitogenic stimulation, Ras and PPP2R1A
    cooperate to release autoinhibition and the subsequent phosphorylation of activating sites: Ser-338, Tyr-341,
    Thr-491, and Ser-494, yields a fully active kinase. Through a negative feedback mechanism involving MAPK1/ERK2,
    RAF1 is phosphorylated on Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2, which yields an
    inactive, desensitized kinase. The signaling-competent conformation of RAF1 is finally re-established by the
    coordinated action of PIN1, a prolyl isomerase that converts pSer and pThr residues from the cis to the trans
    conformation, which is preferentially recognized and dephosphorylated by PPP2R1A. Activated by homodimerization
    and heterodimerization (with BRAF). Also regulated through association with other proteins such as KSR2,
    CNKSR1/CNK1, PEBP1/RKIP, PHB/prohibitin and SPRY4. PEBP1/RKIP acts by dissociating RAF1 from its substrates
    MAP2K1/MEK1 and MAP2K2/MEK2. PHB/prohibitin facilitates the displacement of 14-3-3 from RAF1 by activated Ras,
    thereby promoting cell membrane localization and phosphorylation of RAF1 at the activating Ser-338. SPRY4
    inhibits Ras-independent, but not Ras-dependent, activation of RAF1. CNKSR1/CNK1 regulates Src-mediated RAF1
    activation

         Genatlas biochemistry entry for RAF1:
    murine sarcoma viral (v-raf-1) oncogene homolog 1 (mitogenic factor) interacting with TC21 for activation and
    translocation to plasma membrane

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity TAS8063729
    GO:0004674protein serine/threonine kinase activity IDA19667065
    GO:0004709MAP kinase kinase kinase activity IEA--
    GO:0004713protein tyrosine kinase activity ----
    GO:0005057receptor signaling protein activity ----
         
    Find genes that share ontologies with RAF1           About GenesLikeMe


    Phenotypes:
         4 GenomeRNAi human phenotypes for RAF1:
     Decreased substrate adherent c  Decreased viability after Chla  Increased cell death in HCC-19  Increased mitotic index 

         Selected MGI mutant phenotypes (inferred from 12 alleles(MGI details for Raf1) (see all 20):
     behavior/neurological  cardiovascular system  cellular  craniofacial  embryogenesis 
     growth/size/body  hematopoietic system  homeostasis/metabolism  immune system  integument 
     liver/biliary system  mortality/aging  muscle  nervous system  no phenotypic analysis 

    Find genes that share phenotypes with RAF1           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for RAF1: Raf1tm1Zim Raf1tm2.1Bacc Raf1tm1Ulb Raf1tm1Bacc

       genOway: Develop your customized and physiologically relevant rodent model for RAF1

    miRNA
    Products:
        
    miRTarBase miRNAs that target RAF1:
    hsa-mir-23b-3p (MIRT046343), hsa-mir-7-5p (MIRT004026), hsa-mir-186-5p (MIRT044977), hsa-mir-125b-5p (MIRT004418)

    Block miRNA regulation of human, mouse, rat RAF1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate RAF1 (see all 32):
    hsa-miR-4254 hsa-miR-15a hsa-miR-503 hsa-miR-624* hsa-miR-486-3p hsa-miR-649 hsa-miR-424 hsa-miR-15b
    SwitchGear 3'UTR luciferase reporter plasmidRAF1 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat RAF1

    Gene Editing
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    Clone
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    OriGene clones in human, mouse for RAF1 (see all 10)
    OriGene ORF clones in mouse, rat for RAF1
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    GenScript: all cDNA clones in your preferred vector: RAF1 (NM_002880)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAF1
    Addgene plasmids for RAF1 

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    eBioscience FlowRNA Probe Sets ( VA1-12224) for RAF1 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    RAF1_HUMAN, P04049: Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in
    both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the
    cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its
    mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized
    at the nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    mitochondrion5
    nucleus5
    plasma membrane5
    cytosol4

    Gene Ontology (GO): Selected cellular component terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm ISS--
    GO:0005741mitochondrial outer membrane TAS8929532
    GO:0005794Golgi apparatus IPI17724343
    GO:0005829cytosol TAS--

    Find genes that share ontologies with RAF1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for RAF1 About   (see all 175)  
    See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Immune response Fc epsilon RI pathway
    Immune response Fc epsilon RI pathway0.43
    VEGF - VEGF R2 Signaling Pathways0.35
    Fc epsilon RI signaling pathway0.41
    Fc-epsilon receptor I signaling in mast cells0.34
    VEGF signaling pathway0.39
    2IL-9 Signaling Pathways
    IL-9 Signaling Pathways0.90
    IL-4 Signaling Pathways0.84
    IL-7 Signaling Pathways0.90
    Immune response IL 9 signaling pathway0.42
    IL-21 Signaling Pathways0.90
    IL-7 Signaling Pathway0.40
    Common Cytokine Receptor Gamma-Chain Family Signaling Pathways0.90
    Development Thrombopoietin regulated cell processes0.38
    3Translation Insulin regulation of translation
    Translation Insulin regulation of translation0.59
    Cell adhesion PLAU signaling0.35
    Regulation of lipid metabolism Insulin signaling generic cascades0.59
    Transcription PPAR Pathway0.34
    Transcription Receptor mediated HIF regulation0.51
    Development Growth hormone signaling via PI3K AKT and MAPK cascades0.34
    Translation Regulation activity of EIF20.47
    Insulin signaling pathway0.32
    4Development HGF signaling pathway
    Development HGF signaling pathway0.45
    Apoptosis and survival Role of CDK5 in neuronal death and survival0.37
    Development Neurotrophin family signaling0.45
    Neurotrophin signaling pathway0.36
    Development EGFR signaling via small GTPases0.44
    Signaling of Hepatocyte Growth Factor Receptor0.35
    Development PDGF signaling via MAPK cascades0.41
    Apoptosis and survival Anti apoptotic action of membrane bound ESR10.35
    5B cell receptor signaling pathway (KEGG)
    B cell receptor signaling pathway0.42
    T cell receptor signaling pathway0.38
    BCR signaling pathway0.42
    TCR Signaling Pathway0.36


    Find genes that share SuperPaths with RAF1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected R&D Systems Pathways for RAF1 (see all 11)
        IL-21 Signaling Pathways
    IL-2 Signaling Pathways
    IL-4 Signaling Pathways
    Adipocytokines & Insulin Signaling
    Pathogen or Damage-activated C-Type Lectin Receptor Signaling Pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for RAF1 (see all 99)
        Molecular Mechanisms of Cancer
    eIF2 Pathway
    CDK5 Pathway
    JNK Pathway
    NF-kappaB Activation by Viruses

    1 Cell Signaling Technology (CST) Pathway for RAF1
        MAP Kinase Signaling

    3 Tocris Bioscience Pathways for RAF1
        Insulin Pathway
    VEGF Pathway
    MAPK Pathway

    Selected GeneGo (Thomson Reuters) Pathways for RAF1 (see all 89)
        G-protein signaling H-RAS regulation pathway
    Development CNTF receptor signaling
    Apoptosis and survival Anti-apoptotic action of membrane-bound ESR1
    Development EGFR signaling pathway
    Immune response IL-9 signaling pathway

    Selected BioSystems Pathways for RAF1 (see all 58)
        MAPK signaling pathway
    MicroRNAs in cardiomyocyte hypertrophy
    Focal Adhesion
    Integrin-mediated cell adhesion
    Senescence and Autophagy

    3 Sino Biological Pathways for RAF1
        EGFR Signaling Pathway
    VEGF Signaling
    TGF-beta Signaling Pathway

    Selected Reactome Pathways for RAF1 (see all 7)
        MEK activation
    Stimuli-sensing channels
    RAF activation
    GP1b-IX-V activation signalling
    RAF phosphorylates MEK

    4 PharmGKB Pathways for RAF1
        EGFR Inhibitor Pathway, Pharmacodynamics
    Sorafenib Pharmacodynamics
    VEGF Signaling Pathway
    Vemurafenib Pathway, Pharmacodynamics

    Selected Kegg Pathways  (Kegg details for RAF1) (see all 48):
        MAPK signaling pathway
    ErbB signaling pathway
    Ras signaling pathway
    Rap1 signaling pathway
    Chemokine signaling pathway

        Pathway & Disease-focused RT2 Profiler PCR Arrays including RAF1 (see all 12): 
              Oncogenes & Tumor Suppressor Genes in human mouse rat
              Insulin Signaling Pathway in human mouse rat
              Multiple Sclerosis in human mouse rat
              VEGF Signaling in human mouse rat
              PI3K-AKT Signaling Pathway in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for RAF1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for RAF1 (P040491, 2, 3 ENSP000002518494) via UniProtKB, MINT, STRING, and/or I2D (see all 247)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    YWHAZP631041, 2, 3, ENSP000003095034EBI-365996,EBI-347088 MINT-8004922 MINT-8005899 MINT-8010094 MINT-8009701 MINT-45799 MINT-19373 MINT-8006572 MINT-8004938 MINT-8006579 MINT-19372 MINT-8004992 MINT-8004961 MINT-8008718 MINT-45800 I2D: score=7 STRING: ENSP00000309503
    HRASP011121, 2, 3, ENSP000003098454EBI-365996,EBI-350145 MINT-6540149 MINT-60984 MINT-7295101 MINT-6802882 MINT-18357 MINT-17120 MINT-6946517 MINT-6540170 MINT-17119 I2D: score=10 STRING: ENSP00000309845
    PEBP1P300861, 2, 3, ENSP000002613134EBI-365996,EBI-716384 MINT-3389495 MINT-3389468 MINT-3389522 MINT-3389581 MINT-3389607 MINT-3389378 MINT-3389550 I2D: score=6 STRING: ENSP00000261313
    BRAFP150561, 2, 3, ENSP000002886024EBI-365996,EBI-365980 MINT-8396824 MINT-8396759 MINT-8396808 MINT-8396838 MINT-8397040 I2D: score=7 STRING: ENSP00000288602
    YWHABP319461, 2, 3, ENSP000003001614EBI-365996,EBI-359815 MINT-8004910 MINT-8004954 MINT-8005906 MINT-8004894 MINT-8009103 I2D: score=5 STRING: ENSP00000300161
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 38):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000165MAPK cascade TAS--
    GO:0000186activation of MAPKK activity TAS--
    GO:0001666response to hypoxia IEA--
    GO:0006468protein phosphorylation TAS8063729
    GO:0006915apoptotic process TAS8929532

    Find genes that share ontologies with RAF1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

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    Browse Small Molecules at EMD Millipore
       Enzo Life Sciences drugs & compounds for RAF1
    Selected ApexBio Compounds for RAF1 (see all 13)     About this table
    CompoundAction CAS #
    AZ 628selective RAF inhibitor[878739-06-1]
    B-Raf inhibitoruseful in the treatment of cancer and proliferative diseases[1315330-11-0]
    BRAF inhibitora potent BRAF inhibitor[918505-61-0]
    CEP-32496Potent BRAF inhibitor[1188910-76-0]
    Dabrafenib Mesylate (GSK-2118436)selective BRAFV600E inhibitor[1195768-06-9]
    GDC-0879Potent and selective B-Raf inhibitor[905281-76-7]
    PF-04880594RAF inhibitor for BRAF/BRAFV599E and c-RAF[1111636-35-1]
    PLX-4720Potent and selective inhibitor of BRAF with V600E mutation[918505-84-7]
    RAF265Potent and selective Raf inhibitor, also inhibits VEGFR[927880-90-8]
    SorafenibMulti-target kinase inhibitor of Raf kinases and tyrosine kinases[284461-73-0]

    Compounds for RAF1 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    GDC 0879Potent B-Raf inhibitor[905281-76-7]
    ZM 336372 Potent, selective c-Raf inhibitor [208260-29-1]
    GW 5074 Potent, selective c-Raf1 kinase inhibitor [220904-83-6]
    SB 590885Potent B-Raf inhibitor[405554-55-4]
    L-779,450 Potent Raf kinase inhibitor[303727-31-3]

    Selected HMDB Compounds for RAF1 (see all 845)    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    DG(14:0/14:0/0:0)DAG(14:0/14:0) (see all 9)----
    DG(14:0/14:1(9Z)/0:0)DAG(14:0/14:1) (see all 15)----
    DG(14:0/15:0/0:0)Diacylglycerol(29:0) (see all 9)----
    DG(14:0/16:0/0:0)DAG(30:0) (see all 9)----
    DG(14:0/16:1(9Z)/0:0)DG(30:1) (see all 15)----
    DG(14:0/18:0/0:0)DG(14:0/18:0) (see all 9)----
    DG(14:0/18:1(11Z)/0:0)Diacylglycerol (see all 15)----
    DG(14:0/18:1(9Z)/0:0)1-myristoyl-2-oleoyl-sn-glycerol (see all 15)----

    3 DrugBank Compounds for RAF1    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Sorafenib-- 284461-73-0targetinhibitor17272980 17266941 16757355 16890795 17296815 20812347
    Dabrafenib-- --targetinhibitor--
    Regorafenib-- 755037-03-7targetinhibitor--

    Selected Novoseek inferred chemical compound relationships for RAF1 gene (see all 127)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    gw-5074 88.1 20 15255937 (3), 16440327 (2), 19288477 (2), 16546979 (1) (see all 14)
    zm 336372 80.8 15 18063082 (3), 16603190 (2), 15956248 (2), 16909118 (1) (see all 5)
    threonine 80.2 136 9689060 (3), 7803760 (3), 7685905 (2), 1461284 (2) (see all 99)
    tyrosine 77.2 270 8622647 (5), 8662746 (5), 7692235 (5), 7542586 (4) (see all 99)
    pd 98,059 76.2 26 12389631 (2), 10727406 (1), 11703098 (1), 14736735 (1) (see all 20)
    geldanamycin 74.5 41 10927059 (4), 9137531 (4), 12471618 (3), 15846067 (2) (see all 19)
    17-(allylamino)-17-demethoxygeldanamycin 73.3 8 9622079 (1), 11280726 (1), 14506167 (1), 18281495 (1) (see all 5)
    serine 72.9 163 7623807 (3), 1645717 (3), 7685905 (2), 9234708 (2) (see all 99)
    sorafenib 72.2 53 19710032 (3), 16890795 (2), 16503817 (1), 17071608 (1) (see all 37)
    radicicol 72.1 11 10979978 (2), 18676857 (1), 10945979 (1), 11306353 (1) (see all 8)



    Find genes that share compounds with RAF1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

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    REFSEQ mRNAs for RAF1 gene: 
    NM_002880.3  

    Unigene Cluster for RAF1:

    V-raf-1 murine leukemia viral oncogene homolog 1
    Hs.159130  [show with all ESTs]
    Unigene Representative Sequence: GU149302
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000251849(uc011aut.2 uc003bxf.4 uc011auu.2) ENST00000442415
    ENST00000432427 ENST00000423275 ENST00000471449 ENST00000465826 ENST00000475353
    ENST00000494557 ENST00000460610 ENST00000491290 ENST00000416093 ENST00000492690
    ENST00000542177 ENST00000534997
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat RAF1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate RAF1 (see all 32):
    hsa-miR-4254 hsa-miR-15a hsa-miR-503 hsa-miR-624* hsa-miR-486-3p hsa-miR-649 hsa-miR-424 hsa-miR-15b
    SwitchGear 3'UTR luciferase reporter plasmidRAF1 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for RAF1
    Predesigned siRNA for gene silencing in human, mouse, rat RAF1
    Clone
    Products:
         
    OriGene clones in human, mouse for RAF1 (see all 10)
    OriGene ORF clones in mouse, rat for RAF1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: RAF1 (NM_002880)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RAF1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAF1
    Addgene plasmids for RAF1 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for RAF1
    OriGene qSTAR qPCR primer pairs in human, mouse for RAF1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat RAF1
      QuantiTect SYBR Green Assays in human, mouse, rat RAF1
      QuantiFast Probe-based Assays in human, mouse, rat RAF1
    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-12224) for RAF1 

    Additional mRNA sequence: 

    AK055589.1 AK124449.1 AK226028.1 AK303561.1 AK303920.1 AK312248.1 BC018119.2 BX648063.1 
    FM209427.1 GU149302.1 X03484.1 

    20 DOTS entries:

    DT.100669838  DT.100669840  DT.100669848  DT.87008986  DT.91838878  DT.75151772  DT.95278225  DT.95278214 
    DT.100669835  DT.120918106  DT.91705198  DT.91993563  DT.92451812  DT.100690754  DT.120918099  DT.120918136 
    DT.91695943  DT.40190406  DT.91826781  DT.95337142 

    Selected AceView cDNA sequences (see all 551):

    BM766547 BM783986 BP374718 AL042845 AA357046 AW515198 BQ684667 AL120772 
    BM920181 BQ932923 CD513500 BQ425911 BU683389 BU527875 D20161 AA306005 
    BX506853 BE887025 BM696301 NM_002880 BU839297 AI151178 CB143673 BG696548 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    RAF1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGTATACAAG
    RAF1 Expression
    About this image


    RAF1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 9) fully expand
     
     Tooth (Integumentary System)    fully expand to see all 3 entries
             Odontoblasts Dentin
     
     Blood (Hematopoietic System)    fully expand to see all 2 entries
             Monocytes Peripheral Blood
     
     Epithelial Cells
             Dental Placode Cells Dental Placode
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Thyroid (Endocrine System)
    RAF1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    RAF1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.159130

    UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049
    Tissue specificity: In skeletal muscle, isoform 1 is more abundant than isoform 2

        Pathway & Disease-focused RT2 Profiler PCR Arrays including RAF1 (see all 12): 
              Oncogenes & Tumor Suppressor Genes in human mouse rat
              Insulin Signaling Pathway in human mouse rat
              Multiple Sclerosis in human mouse rat
              VEGF Signaling in human mouse rat
              PI3K-AKT Signaling Pathway in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for RAF1
    OriGene qSTAR qPCR primer pairs in human, mouse for RAF1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat RAF1
    QuantiTect SYBR Green Assays in human, mouse, rat RAF1
    QuantiFast Probe-based Assays in human, mouse, rat RAF1
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAF1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

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    This gene was present in the common ancestor of animals.

    Orthologs for RAF1 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Raf11 , 5 v-raf-leukemia viral oncogene 11, 5 90.79(n)1
    97.69(a)1
      6 (53.62 cM)5
    1101571  NM_029780.31  NP_084056.11 
     1156180675 
    chicken
    (Gallus gallus)
    Aves RAF11 v-raf-1 murine leukemia viral oncogene homolog 1 81.4(n)
    94.59(a)
      396245  NM_205307.2  NP_990638.1 
    lizard
    (Anolis carolinensis)
    Reptilia RAF16
    v-raf-1 murine leukemia viral oncogene homolog 1
    85(a)
    1 ↔ 1
    2(158964683-159074372)
    African clawed frog
    (Xenopus laevis)
    Amphibia c-raf2 raf protein 76.42(n)    S74063.1 
    zebrafish
    (Danio rerio)
    Actinopterygii craf2 murine leukemia viral oncogene homolog 74.13(n)   30716  BC054662.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta phl3 dorsal/ventral axis determination,
    follicular more
    47(a)   1 3A1   --
    worm
    (Caenorhabditis elegans)
    Secernentea lin-453 Expression: head neurons, neurons 54(a)   IV(6743426-6747592)   --


    ENSEMBL Gene Tree for RAF1 (if available)
    TreeFam Gene Tree for RAF1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for RAF1 gene
    FPGT-TNNI3K2  MAP3K122  MAP3K112  KIAA18042  KSR22  KSR12  MOS2  BRAF2  
    MAP3K92  MAP3K102  ARAF2  MAP3K72  TNNI3K2  ZAK2  MAP3K132  ILK2  
    17 SIMAP similar genes for RAF1 using alignment to 6 protein entries:     RAF1_HUMAN (see all proteins):
    BRAF    ARAF    NUAK1    CDK4    STK11    STK36
    TXK    STK25    DKFZp564L2416    FRK    RPS6KA2    ZAK
    MAP4K3    FGR    PAK2    FES    SRGAP3

    Find genes that share paralogs with RAF1           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for RAF1
    PGOHUM00000245458


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for RAF1 (see all 1969)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0378204
    Noonan syndrome 5 (NS5)4--see VAR_0378202 S T mis40--------
    VAR_0378144
    Noonan syndrome 5 (NS5)4--see VAR_0378142 P S mis40--------
    VAR_0378114
    Noonan syndrome 5 (NS5)4--see VAR_0378112 T R mis40--------
    VAR_0410374
    An ovarian serous carcinoma sample4--see VAR_0410372 S A mis40--------
    VAR_0378084
    Noonan syndrome 5 (NS5)4--see VAR_0378082 S L mis40--------
    VAR_0378174
    Noonan syndrome 5 (NS5)4--see VAR_0378172 D N mis40--------
    VAR_0378214
    Noonan syndrome 5 (NS5)4--see VAR_0378212 L V mis40--------
    VAR_0378134
    Noonan syndrome 5 (NS5)4--see VAR_0378132 P L mis40--------
    VAR_0378094
    Noonan syndrome 5 (NS5)4--see VAR_0378092 S F mis40--------
    VAR_0378124
    Noonan syndrome 5 (NS5)4--see VAR_0378122 P A mis40--------

    HapMap Linkage Disequilibrium report for RAF1 (12625100 - 12705725 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 8 variations for RAF1:    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv850e199CNV Deletion23128226
    dgv810e201CNV Deletion23290073
    esv25162CNV Loss19812545
    nsv470394CNV Gain18288195
    nsv876541CNV Gain21882294
    esv2751977CNV Gain17911159
    dgv597n27CNV Gain19166990
    nsv7351OTHER Inversion18451855

    Human Gene Mutation Database (HGMD): RAF1
    Site Specific Mutation Identification with PCR Assays
    2 Copy Number PCR Panels containing RAF1:
    Oncogenes & Tumor Suppressor Genes 384HC
    Prostate Cancer
    SeqTarget long-range PCR primers for resequencing RAF1
    DNA2.0 Custom Variant and Variant Library Synthesis for RAF1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

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    OMIM gene information: 164760   
    OMIM disorders: 611553  611554  
    UniProtKB/Swiss-Prot: RAF1_HUMAN, P04049
  • Noonan syndrome 5 (NS5) [MIM:611553]: A form of Noonan syndrome, a disease characterized by short
    stature, facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated
    ears, and a high incidence of congenital heart defects and hypertrophic cardiomyopathy. Other features can
    include a short neck with webbing or redundancy of skin, deafness, motor delay, variable intellectual deficits,
    multiple skeletal defects, cryptorchidism, and bleeding diathesis. Individuals with Noonan syndrome are at risk
    of juvenile myelomonocytic leukemia, a myeloproliferative disorder characterized by excessive production of
    myelomonocytic cells. Note=The disease is caused by mutations affecting the gene represented in this entry
  • LEOPARD syndrome 2 (LEOPARD2) [MIM:611554]: A disorder characterized by lentigines, electrocardiographic
    conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of
    growth, and sensorineural deafness. Note=The disease is caused by mutations affecting the gene represented in
    this entry

  • 19 diseases for RAF1:    
    About MalaCards
    raf1-related leopard syndrome    noonan syndrome 5    raf1-related noonan syndrome    leopard syndrome
    leopard syndrome 1    kyasanur forest disease    leopard syndrome 2    murray valley encephalitis
    west nile fever    noonan syndrome    st. louis encephalitis    spiradenoma
    noonan syndrome 1    pilocytic astrocytoma    cardiomyopathy, dilated, 1w    lung adenoma
    dengue disease    cardiofaciocutaneous syndrome    encephalitis

    10 diseases from the University of Copenhagen DISEASES database for RAF1:
    Yellow fever     Hepatitis     tick-borne encephalitis     Hepatitis C
    Dengue disease     Murray Valley encephalitis     St. Louis encephalitis     West Nile fever
    Kyasanur forest disease     Encephalitis

    Find genes that share disorders with RAF1           About GenesLikeMe

    Selected Novoseek inferred disease relationships for RAF1 gene (see all 85)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    noonan syndrome 58.6 7 19953625 (2), 17603483 (2), 20052757 (1), 20461756 (1) (see all 5)
    cancer 55.9 83 12781369 (3), 19715444 (2), 18221149 (2), 17555829 (2) (see all 67)
    tumors 53 192 16926634 (6), 8451761 (3), 15803144 (3), 10830717 (3) (see all 99)
    viral leukemia 46.5 3 1783381 (1), 15845465 (1), 10449917 (1)
    shock 45.8 28 9622079 (1), 11280726 (1), 14724288 (1), 15338192 (1) (see all 26)
    melanoma 38.2 31 19737955 (8), 17018604 (3), 17699719 (2), 14654778 (2) (see all 15)
    thyroid carcinoma medullary 36.1 8 16926634 (4), 17363508 (2), 15572361 (1), 9690518 (1)
    leukemia 34.6 22 16250879 (3), 12903397 (3), 9632143 (2), 9242431 (1) (see all 14)
    solid tumor 34 5 15585643 (1), 20178713 (1), 16498671 (1), 16890795 (1)
    breast cancer 33.2 30 7834453 (4), 12432273 (2), 16613992 (2), 11796827 (2) (see all 20)

    GeneTests: RAF1
    GeneReviews: RAF1
    Genetic Association Database (GAD): RAF1
    Human Genome Epidemiology (HuGE) Navigator: RAF1 (14 documents)
    Tumor Gene Database (TGDB): RAF1

    Export disorders for RAF1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for RAF1 gene, integrated from 10 sources (see all 1589):
    (articles sorted by number of sources associating them with RAF1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1. (PubMed id 11733498)1, 2, 9 Zang M.... Luo Z. (J. Biol. Chem. 2002)
    2. Retinoic acid induces nuclear accumulation of Raf1 during differentiation of HL-60 cells. (PubMed id 19298812)1, 2, 9 Smith J....Yen A. (Exp. Cell Res. 2009)
    3. p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association. (PubMed id 15849194)1, 2, 9 Jin S.... Field J. (J. Biol. Chem. 2005)
    4. Raf-1 promotes cell survival by antagonizing apoptosis signal- regulating kinase 1 through a MEK-ERK independent mechanism. (PubMed id 11427728)1, 2, 9 Chen J.... Fu H. (Proc. Natl. Acad. Sci. U.S.A. 2001)
    5. Investigation of gene dosage imbalances in patients with Noonan syndrome using multiplex ligation-dependent probe amplification analysis. (PubMed id 20302979)1, 4, 9 NystrAPm A.M....Bondeson M.L. (Eur J Med Genet 2010)
    6. Diacylglycerol kinase eta augments C-Raf activity and B-Raf/C-Raf heterodimerization. (PubMed id 19710016)1, 2, 9 Yasuda S.... Sakane F. (J. Biol. Chem. 2009)
    7. Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding. (PubMed id 16093354)1, 2, 9 Zhu J.... Tzivion G. (Mol. Biol. Cell 2005)
    8. Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1. (PubMed id 12717443)1, 2, 9 Sasaki A.... Yoshimura A. (Nat. Cell Biol. 2003)
    9. The solution structure of the Raf-1 cysteine-rich domain: a novel ras and phospholipid binding site. (PubMed id 8710867)1, 2, 9 Mott H.R....Campbell S.L. (Proc. Natl. Acad. Sci. U.S.A. 1996)
    10. Phosphorylation of Raf by ceramide-activated protein kinase. (PubMed id 7477354)1, 2, 9 Yao B.... Kolesnick R. (Nature 1995)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 5894 HGNC: 9829 AceView: RAF1 Ensembl:ENSG00000132155 euGenes: HUgn5894
    ECgene: RAF1 Kegg: 5894 H-InvDB: RAF1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for RAF1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for RAF1 Genetics and Cytogenetics in Oncology and Haematology
    GeneReviewshttp://www.ncbi.nlm.nih.gov/books/NBK1116/?term=RAF1[genesymbol]
    NIEHS-SNPshttp://egp.gs.washington.edu/data/raf1/

    (Patent information from GeneIP,
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    Patent Information for RAF1 gene:
    Search GeneIP for patents involving RAF1

    GeneCards and IP:
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