 |
 | Services
| |
 |
Aliases &
Descriptions
for RAD9A
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases |
|---|
| EC 3.1.11.2 3 | | RAD9 2, 5 | | hRAD9 3 |
| | | Descriptions |
|---|
| DNA repair exonuclease rad9 homolog A 3 | | RAD9 (S. pombe) homolog 1 | | RAD9 homolog 2 | | RAD9 homolog A (S. pombe) 2 | | cell cycle checkpoint control protein 2 |
|
| |
Search outside databases for aliases for RAD9A genePrevious GC identifers: GC11P067409 GC11P066934 |
Summaries
for RAD9A(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for RAD9A:
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint
protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This
protein is found to possess 3' to 5' exonuclease activity, which may contribute to its
role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and
HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is
thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA
sites has been noted for this gene. [provided by RefSeq]
UniProtKB/Swiss-Prot: RAD9A_HUMAN, Q99638Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in
DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication
factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several
proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA
polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the
primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1
cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths;
and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5'
double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the
formation of the 9-1-1 complexGene Wiki entry for RAD9A |
Genomic Location
for RAD9A
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences)
About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
 Transcription factor binding sites upstream to the RAD9A gene 
Entrez Gene cytogenetic band: 11q13.1-q13.2 Ensembl cytogenetic band: 11q13.1 HGNC cytogenetic band: 11q13.1-q13.2RAD9A Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11P066915:
(about GC identifiers)
Start:
|
66,915,999 bp from pter |
End:
|
66,922,459 bp from pter |
Size:
|
6,461 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000011.8 NT_033903.7
| Proteins
for RAD9A
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: RAD9A_HUMAN, Q99638 (See
protein sequence)Recommended Name: Cell cycle checkpoint control protein RAD9A Size: 391 amino acids; 42547 Da
Subunit: Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and
HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1
complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B,
HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2
Subcellular location: Nucleus
Secondary accessions: Q6FI29 Q96C41Post-translational modifications:
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage.
Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be
required for the formation of the 9-1-1 complex1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_004575.1
ENSEMBL proteins: ENSP00000311360
Human Recombinant Proteins  OriGene Purified Recombinant Human Protein: RAD9A
3 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for RAD9A:
Assays for RAD9A: | Protein
Domains/
Families
for RAD9A(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q99638
ProtoNet protein and cluster: Q99638 UniProtKB/Swiss-Prot: RAD9A_HUMAN, Q99638Similarity: Belongs to the rad9 family | Gene Function
for RAD9A
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004584
 Applied Biosystems Silencer® siRNAs for RAD9A
 Sigma-Aldrich siRNA and siRNA Panels for RAD9A
Sigma-Aldrich shRNA Panels and shRNA for RAD9A
Explore Sigma-Aldrich super-pooled esiRNAs
OriGene GFP tagged cDNA clone in CMV expression vector: NM_004584
Myc/DDK tagged cDNA clone in CMV expression vector: NM_004584
untagged cDNA clones in CMV expression vector (see all 2): NM_004584
Primers:  Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
OriGene genome-wide validated SYBR primer pairs: NM_004584
UniProtKB/Swiss-Prot: RAD9A_HUMAN, Q99638Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in
DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication
factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several
proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA
polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the
primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1
cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths;
and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5'
double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the
formation of the 9-1-1 complexCatalytic activity: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside
5'-phosphatesEnzyme Number (IUBMB): EC 3.1.11.2 7 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for Rad9):
5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6
): About this table | Pathways & Interactions
for RAD9A
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
2 Sigma-Aldrich "Your Favorite Gene" Pathways for RAD9A (Your Favorite Gene powered by Ingenuity)
Gene Network CentralTM Interacting Genes and Proteins Network for RAD9A
5/34 Interacting proteins for RAD9A (ENSP000003113603 Q996382) via UniProtKB, MINT, and/or STRING (see all 34
)About this table
5/7 Gene Ontology (GO) biological process terms (links to tree view) (see all 7
): About this table
|
Drugs & Compounds
for RAD9A(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for RAD9A
|
Transcripts
for RAD9A(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene,
Expression Assays from Applied Biosystems)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004584
Sigma-Aldrich siRNA and siRNA Panels for RAD9A
Sigma-Aldrich shRNA Panels and shRNA for RAD9A
Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer® siRNAs: NM_004584 REFSEQ mRNAs for RAD9A gene: NM_004584.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_004584
OriGene GFP tagged cDNA clone in CMV expression vector: NM_004584
Myc/DDK tagged cDNA clone in CMV expression vector: NM_004584
untagged cDNA clones in CMV expression vector (see all 2): NM_004584
Additional cDNA sequence: AK310213.1 AK315348.1 BC014848.1 CR536508.1 CR624269.1 U53174.1 16 DOTS entries: DT.92465301 DT.100884967 DT.100760796 DT.450687 DT.100884968 DT.100009273 DT.40133542 DT.92465318 DT.100019025 DT.100884978 DT.102838908 DT.92465284 DT.92465297 DT.92465314 DT.97855870 DT.100884945 24/127 AceView cDNA sequences (see all 127
):BQ026878 BE742484 BQ707208 CR536508 AK096155 T16617 BM918589 AI261247 BE293551 BC014848 AA657789 BG258756 BF915070 CA307911 NM_004584 BX093492 BE260538 BM845563 BQ882681 U53174 AI024820 CR624269 BX110033 BE249853
 highest scoring ESTs for RAD9A:U53174 AA329958 AA513749 AA568365 AA708970 AA909447 AA922612 AA928674 AI024820 AI261247 Unigene Cluster for RAD9A: RAD9 homolog A (S. pombe) Hs.655354 [show with all ESTs]Unigene Representative Sequence: NM_004584
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for RAD9A
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6 | ^ | 7a | · | 7b | ^ | 8 | ^ | 9 | ^ | 10 |
|---|
|
| SP1: | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for RAD9A
1 Ensembl transcript including schematic representation: ENST00000307980
|
Expression
for RAD9A
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| RAD9A expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for RAD9A
1 / 2 / 3 5 probe-sets matching RAD9A gene Data from
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: GCGAGAATCC
SOURCE GeneReport for Unigene cluster: Hs.655354
Expression variation in blood from EXPOLDB for RAD9A |
Orthologs
for RAD9A
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for RAD9A gene from 5/10 species (see all 10
)
About this table Species with no ortholog for RAD9A
ENSEMBL Gene Tree for RAD9A | Paralogs
for RAD9A(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for RAD9A gene
- RAD9B2
|
SNPs/Variants
for RAD9A(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for RAD9A (up to first 250kb)
|
Disorders & Mutations
for RAD9A
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 603761
5  Novoseek disease relationships for RAD9A gene
About this table
Human Gene Mutation Database: RAD9A
|
Medical News
for RAD9A(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for RAD9A (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/100 PubMed articles for RAD9A gene (see all 100
):- A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene. (PubMed id 8943031)2, 3, 4 Lieberman H.B.... Davey S. (1996)
- Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling. (PubMed id 12709442)1, 3, 4 Roos-Mattjus P.... Karnitz L.M. (2003)
- Protein kinase Cdelta is responsible for constitutive and DNA damage- induced phosphorylation of Rad9. (PubMed id 12628935)1, 3, 4 Yoshida K.... Kufe D. (2003)
- Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue. (PubMed id 14500360)1, 3, 4 Hopkins K.M....Lieberman H.B. (2003)
- c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage. (PubMed id 11971963)1, 3, 4 Yoshida K.... Kufe D. (2002)
- Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease. (PubMed id 10713044)1, 3, 4 Bessho T. and Sancar A. (2000)
- The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9. (PubMed id 10884395)1, 3, 4 Rauen M.... Karnitz L.M. (2000)
- The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1. (PubMed id 10359610)1, 3, 4 St Onge R.P.... Davey S. (1999)
- HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. (PubMed id 10846170)1, 3, 4 Cai R.L.... Cohen D. (2000)
- Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. (PubMed id 15897895)1, 3, 4 Wu X.... Zou Y. (2005)
|
Search for RAD9A
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing RAD9A
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing RAD9A
(According to HUGE)
About This Section
| -- |
Specialized Databases showing RAD9A(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| About This Section
| --
| Services
for RAD9A(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section
| 
Products for RAD9A:
 | |
 | | | | |
 |
 |  |  | |
|  | Recombinant Proteins
(Rad9) | | | Antibodies (Rad9) |
| | | | Search Tocris compounds for RAD9A |
|
 |
 | | |
|
GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
|
V3 here soon-try beta now
