Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RAD21 Gene

protein-coding   GIFtS: 66
GCID: GC08M117858

RAD21 homolog (S. pombe)

(Previous name: RAD21 (S. pombe) homolog )
 Explore 21 diseases affiliated with
RAD21 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for RAD21    

Aliases
for RAD21 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
RAD21 Homolog (S. Pombe)1 2     SCC1 Homolog2 3
HHR211     RAD21 (S. Pombe) Homolog1
SCC11 2 5     CDLS42
KIAA00781 3 5     HRAD212
NXP12 3 5     MCD12
Nuclear Matrix Protein 12 3     Double-Strand-Break Repair Protein Rad21 Homolog2
HR212 3     Protein Involved In DNA Double-Strand Break Repair2
NXP-12 3     

External Ids:    HGNC: 98111   Entrez Gene: 58852   Ensembl: ENSG000001647547   OMIM: 6064625   UniProtKB: O602163   

Export aliases for RAD21 gene to outside databases

Previous GC identifers: GC08M116846 GC08M117919 GC08M117526 GC08M117814 GC08M117927 GC08M113184


Summaries
for RAD21 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for RAD21:
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene
involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a
nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of
this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid
cohesion in mitotic cells. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: RAD21_HUMAN, O60216
Function: Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair,
and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The
cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At
metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing
sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also
plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the
C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the
apoptotic pathway

Gene Wiki entry for RAD21


Genomic Views
for RAD21 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000008.10  NC_018919.1  NT_008046.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RAD21 gene promoter:
         RFX1   FOXF2   Evi-1   PPAR-alpha   POU2F1   POU2F1a   FOXJ2 (long isoform)   FOXJ2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 6): RAD21 promoter sequence
   Search SABiosciences Chromatin IP Primers for RAD21

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAD21


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24   Ensembl cytogenetic band:  8q24.11   HGNC cytogenetic band: 8q24.11

RAD21 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RAD21 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M117858:  view genomic region     (about GC identifiers)

Start:
 117,858,173 bp from pter      End:
 117,887,105 bp from pter
Size:
 28,933 bases      Orientation:
 minus strand

Proteins
for RAD21 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: RAD21_HUMAN, O60216 (See protein sequence)
Recommended Name: Double-strand-break repair protein rad21 homolog  
Size: 631 amino acids; 71690 Da
Subunit: Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge
domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Found in a
complex with SMC1A, SMC3, CDCA5, PDS5A/APRIN and PDS5B/SCC-112. Interacts with PDS5B and WAPAL; the interaction is
direct
Subcellular location: Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it
is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably
because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved
by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once
cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger
apoptosis
Sequence caution: Sequence=BAA07554.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: A8K0E0 Q15001 Q99568

Explore the universe of human proteins at neXtProt for RAD21: NX_O60216

Post-translational modifications:

  • Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis.
    • The cleavage by ESPL1 and caspase-3 take place at different sites1
  • Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome
    • arms during prophase may be partly due to its phosphorylation by PLK1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O60216

    • RAD21 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_006256.1  
    ENSEMBL proteins: 
     ENSP00000297338   ENSP00000428513   ENSP00000430273   ENSP00000428003   ENSP00000429342  
     ENSP00000427923   ENSP00000430524   ENSP00000428158  
    Reactome Protein details: O60216
    Human Recombinant Protein Products: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate: RAD21
    OriGene Custom Protein Services for RAD21 
    GenScript Custom Purified and Recombinant Proteins Services for RAD21
    Novus Biologicals RAD21 Protein
    Novus Biologicals RAD21 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for RAD21

    Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000228nuclear chromosome ----
    GO:0000775chromosome, centromeric region IEA--
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005730NOT nucleolus IDA--


    RAD21 for ontologies           About GeneDecksing



    RAD21 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of RAD21
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for RAD21 
    OriGene Antibodies: RAD21
    OriGene Custom Antibody Services for RAD21 
    GenScript Custom Superior Antibodies Services for RAD21
    Novus Biologicals RAD21 Antibodies
    Abcam antibodies for RAD21 
    Uscn Antibodies for RAD21
    ThermoFisher Antibodies for RAD21

    Assay Products for RAD21: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Immunoassay Development
    Browse OriGene Fluorogenic Cell Assay Kits
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for RAD21
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for RAD21

    Protein Domains /
    Families
    for RAD21 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    RAD21 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR023093 Rad21/Rec8_C
     IPR006909 Rad21/Rec8_C_eu
     IPR006910 Rad21_Rec8_N

    Graphical View of Domain Structure for InterPro Entry O60216

    ProtoNet protein and cluster: O60216

    UniProtKB/Swiss-Prot: RAD21_HUMAN, O60216
    Domain: The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3 (By
    similarity)
    Similarity: Belongs to the rad21 family


    Function
    for RAD21 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: RAD21_HUMAN, O60216
    Function: Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair,
    and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The
    cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At
    metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing
    sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also
    plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the
    C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the
    apoptotic pathway

         Genatlas biochemistry entry for RAD21:
    yeast (S pombe),RAD21 homolog,highly expressed in testis,thymus,involved in nucleotide excision repair of ionizing
    radiation induced DNA double-strand break

    miRNA
    Products:            		 OriGene 3'-UTR Clone: RAD21
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RAD21
    8/27 QIAGEN miScript miRNA Assays for microRNAs that regulate RAD21 (see all 27):
    hsa-miR-320a hsa-miR-16-2* hsa-miR-181c hsa-miR-25 hsa-let-7a-2* hsa-miR-511 hsa-let-7g* hsa-miR-18b*
    SwitchGear 3'UTR luciferase reporter plasmid: RAD21 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAD21 (see all 7)
    OriGene shRNA RFP: RAD21
    OriGene siRNA: RAD21
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAD21

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for RAD21

    Clone
    Products:             		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAD21 (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAD21 (see all 2)
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: RAD21 (NM_006265)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RAD21
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAD21 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for RAD21
    Search LifeMap BioReagents cell lines for RAD21

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAD21

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI--


    RAD21 for ontologies           About GeneDecksing


    5 GenomeRNAi human phenotypes for RAD21:
     Decreased TP53 protein express  Increased ID2::GFP protein exp  Increased cell death in breast  Low eccentricity cells 
     Upregulation of Wnt/beta-caten 

    Animal Models:
         5 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Rad21):
     cellular  hematopoietic system  immune system  mortality/aging  reproductive system 

    RAD21 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for RAD21 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/11 super-pathways (see all 11) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1M Phase
    		M Phase1.00
    		Mitotic Anaphase0.85
    		Mitotic M-M/G1 phases0.88
    		Separation of Sister Chromatids0.80
    		Mitotic Metaphase and Anaphase0.85
    2Meiotic Synapsis
    		Meiotic Synapsis1.00
    		Meiosis0.70
    		Telomere clustering at the nuclear membrane0.82
    		Chromosome Maintenance0.56
    3Cohesin Loading onto Chromatin
    		Cohesin Loading onto Chromatin1.00
    		Establishment of Sister Chromatid Cohesion0.62
    		Mitotic Telophase/Cytokinesis0.71
    4Resolution of Sister Chromatid Cohesion
    		Resolution of Sister Chromatid Cohesion1.00
    		Mitotic Prometaphase0.93
    		Kinetochore assembly0.96
    5Cell cycle_Spindle assembly and chromosome separation
    		Cell cycle_Spindle assembly and chromosome separation1.00
    		Cell cycle Spindle assembly and chromosome separation0.94

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    1 EMD Millipore Pathway for RAD21
        Cell cycle Spindle assembly and chromosome separation


    2 Cell Signaling Technology (CST) Pathways for RAD21
        Chromatin Regulation / Acetylation
    Cell Cycle / Checkpoint Control

    1 GeneGo (Thomson Reuters) Pathway for RAD21
        Cell cycle Spindle assembly and chromosome separation

    5/20        Reactome Pathways for RAD21 (see all 20)
        Establishment of Sister Chromatid Cohesion
    Mitotic Prometaphase
    Resolution of Sister Chromatid Cohesion
    Cell Cycle
    M Phase


    1         Kegg Pathway  (Kegg details for RAD21):
        Cell cycle


    RAD21 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RAD21

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/652 Interacting proteins for RAD21 (O602161, 2, 3 ENSP000002973384) via UniProtKB, MINT, STRING, and/or I2D (see all 652)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SMC3Q9UQE71, 2, 3, ENSP000003547204EBI-80739,EBI-80718 MINT-6178106 MINT-8081286 MINT-8385079 MINT-8081321 MINT-7914988 MINT-6178206 MINT-7945693 MINT-8080160 MINT-6178091 MINT-8081303 MINT-8385100 I2D: score=3 STRING: ENSP00000354720
    SSU72Q9NP772, 3, ENSP000002913864MINT-8081286 MINT-8081321 MINT-8081344 MINT-8081406 MINT-8081393 MINT-8080160 MINT-8081303 MINT-8081377 MINT-8080156 I2D: score=2 STRING: ENSP00000291386
    STAG2Q8N3U42, 3, ENSP000002180894MINT-6178106 MINT-8081286 MINT-8081321 MINT-7914988 MINT-8080160 MINT-8081377 I2D: score=9 STRING: ENSP00000218089
    STAG1Q8WVM72, 3, ENSP000003726894MINT-6178106 MINT-6178206 MINT-7945693 MINT-6178091 I2D: score=4 STRING: ENSP00000372689
    SMC2O953472, 3, ENSP000002863984MINT-7945693 I2D: score=2 STRING: ENSP00000286398
    About this table

    Gene Ontology (GO): 5/12 biological process terms (GO ID links to tree view) (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000087M phase of mitotic cell cycle TAS--
    GO:0000236mitotic prometaphase TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0006302double-strand break repair TAS8812457
    GO:0006310DNA recombination TAS8812457


    RAD21 for ontologies           About GeneDecksing



    Drugs & Compounds
    for RAD21 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    RAD21 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RAD21
    1 Novoseek chemical compound relationship for RAD21 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    glycolipid 45 1 9820479 (1)

    Search CenterWatch for drugs/clinical trials and news about RAD21 

    Transcripts
    for RAD21 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for RAD21 gene: 
    NM_006265.2  

    Unigene Cluster for RAD21:

    RAD21 homolog (S. pombe)
    Hs.81848  [show with all ESTs]
    Unigene Representative Sequence: NM_006265
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000297338(uc003yod.3) ENST00000523986 ENST00000517749 ENST00000518055
    ENST00000520992 ENST00000517485 ENST00000523547 ENST00000519837 ENST00000522699
    ENST00000519469

    miRNA
    Products:             		 OriGene 3'-UTR Clone: RAD21
    Browse OriGene MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat RAD21
    8/27 QIAGEN miScript miRNA Assays for microRNAs that regulate RAD21 (see all 27):
    hsa-miR-320a hsa-miR-16-2* hsa-miR-181c hsa-miR-25 hsa-let-7a-2* hsa-miR-511 hsa-let-7g* hsa-miR-18b*
    SwitchGear 3'UTR luciferase reporter plasmid: RAD21 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAD21 (see all 7)
    OriGene shRNA RFP: RAD21
    OriGene siRNA: RAD21
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAD21
    Clone
    Products:             		 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAD21 (see all 3)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAD21 (see all 2)
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: RAD21 (NM_006265)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for RAD21
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAD21 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAD21
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RAD21
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAD21
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAD21

    Additional cDNA sequence: 

    AK097915.1 AK098521.1 AK125620.1 AK289505.1 BC001229.2 BC050381.1 D38551.1 X98294.1 

    24/29 DOTS entries (see all 29):

    DT.97771208  DT.100670854  DT.452774  DT.100670857  DT.92414550  DT.92412530  DT.92464093  DT.100670867 
    DT.95095804  DT.121468904  DT.95168507  DT.100670863  DT.95325953  DT.95168515  DT.121468959  DT.100670866 
    DT.91952378  DT.100723214  DT.121468816  DT.121468905  DT.92393545  DT.95083595  DT.100044821  DT.121468853 

    24/837 AceView cDNA sequences (see all 837):

    AA376736 AI627668 CA443072 BM474979 BQ228819 CD358988 BM833822 AA699622 
    CB122036 BM678541 BM721247 BM476529 BQ638398 BI491737 AA872152 BQ425764 
    CA390362 AK125620 BM466374 T35554 BU507348 BP343593 AL705600 AI905425 

    GeneLoc Exon Structure

    5/9 Alternative Splicing Database (ASD) splice patterns (SP) for RAD21 (see all 9)    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b · 3c · 3d ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8a · 8b ^ 9a · 9b · 9c ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b · 14c ^ 15a · 15b ^
    SP1:        -     -     -     -     -     -                                                                                         -     -                     
    SP2:                          -     -     -                                                                                                                     
    SP3:                                                                                                                                                            
    SP4:              -     -     -     -     -                       -     -     -     -     -     -     -     -     -     -     -     -     -     -     -     -   
    SP5:              -     -     -     -     -                                                                                                                     

    ExUns: 16a · 16b ^ 17a · 17b · 17c
    SP1:                              
    SP2:                              
    SP3:                              
    SP4:  -                           
    SP5:                              


    ECgene alternative splicing isoforms for RAD21

    Expression
    for RAD21 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RAD21 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGACTGTTAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    RAD21 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    2 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    Skeletal MuscleHyoid Arch MusclesMyoblastsSkeletal Muscle
    Skeletal MuscleMandibular Arch MusclesMyoblastsSkeletal Muscle
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See RAD21 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RAD21

    SOURCE GeneReport for Unigene cluster: Hs.81848
        SABiosciences Expression via Pathway-Focused PCR Arrays including RAD21: 
              Cell Cycle in human mouse rat
              TGFB Signaling Targets in human mouse rat
              DNA Damage Signaling Pathway in human mouse rat
              DNA Repair in human mouse rat

    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAD21
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat RAD21
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAD21
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAD21
    In Situ
    Assay Products:     
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAD21

    Orthologs
    for RAD21 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of eukaryotes.

    Orthologs for RAD21 gene from 9/29 species (see all 29)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    mouse
    (Mus musculus)    		 Mammalia Rad211 , 5 RAD21 homolog (S. pombe)1, 5 87.8(n)1
    97.31(a)1    		   15 (19.54 cM)5
    193571  NM_009009.41  NP_033035.31 
     519626055 
    chicken
    (Gallus gallus)    		 Aves RAD211 RAD21 homolog (S. pombe) 83.02(n)
    93.49(a)    		   420286  NM_001030950.1  NP_001026121.1 
    lizard
    (Anolis carolinensis)    		 Reptilia RAD216
    		 --
    		 92(a)
    		 1 ↔ 1
    		 4(7058250-7078115)
    African clawed frog
    (Xenopus laevis)    		 Amphibia Xl.60462 Xenopus laevis 14S cohesin RAD21 subunit mRNA, complete more 79.75(n)    AF051786.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii rad212 RAD21 homolog (S. pombe) 76.37(n)   322275  BC045311.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta Rad213
    vtd1    		 mitotic sister chromatid cohesion3
    verthandi1    		 37(a)3
    52.28(n)1
    46.71(a)1    		   33548961  NM_001015132.11  NP_001015132.11 
    worm
    (Caenorhabditis elegans)    		 Secernentea coh-11 , 3 S. pombe double-strand-break repair protein,
    RAD213
    Protein COH-11    		 34(a)
    (best of 2)3
    46.76(n)1
    36.22(a)1    		   1810031  NM_076861.21  NP_509262.11 
    thale cress
    (Arabidopsis thaliana)    		 eudicotyledons ATRAD21.26
    ATREC86
    (see all 4)    		 Sister chromatid cohesion 1 protein 1
    (see all 4)    		 16(a)
    15(a)
    (see all 4)    		 many ↔ many
    many ↔ many
    (see all 4)    		 3(21997054-22000678)
    5(1624206-1629269)
    rice
    (Oryza sativa)    		 Liliopsida --
    --
    (see all 4)    		 Rad21 / Rec8 like protein, putative, expressed
    (see all 4)    		 15(a)
    15(a)
    (see all 4)    		 many ↔ many
    many ↔ many
    (see all 4)    		 8(10159235-10164703)
    5(28826977-28834353)


    ENSEMBL Gene Tree for RAD21 (if available)
    TreeFam Gene Tree for RAD21 (if available) 

    Paralogs
    for RAD21 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RAD21 gene
    RAD21L12  
    2 SIMAP similar genes for RAD21 using alignment to 8 protein entries:     RAD21_HUMAN (see all proteins):
    RAD21L    RAD21L1

    RAD21 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for RAD21
    PGOHUM00000241906


    Genomic Variants
    for RAD21 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    UniProtKB/Swiss-Prot: RAD21_HUMAN, O60216
    Polymorphism: Some radiosensitive cancer patients seem to have Arg-481 instead of the conserved Gly-481. It may be that
    this mutation could contribute to radiosensitivity


    10/642 NCBI SNPs in RAD21 are shown (see all 642)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 8 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs791647721,2
    		F,--113184365(+) TATTTA/GTTAAA 1 -- ds50011Minor allele frequency- G:0.09WA 118
    rs766771071,2
    		--113184504(+) GGCAAA/CCAAAC 1 -- ds50012Minor allele frequency- C:0.11CSA WA 120
    rs115487311,2
    		C--113184658(+) AGAACA/GATATT 1 -- ut312Minor allele frequency- G:0.00NA 4
    rs169185791,2
    		F,--113184951(-) AAAAAA/-TCACT 1 -- ut31 trp31Minor allele frequency- -:0.02NS 176
    rs1138368371,2
    		--113185150(+) TGCAAC/TGCTAT 1 -- ut312Minor allele frequency- T:0.10CSA WA 120
    rs733102061,2
    		C,--113185232(+) GAAGCC/TGTTAG 1 -- ut311Minor allele frequency- T:0.50WA 2
    rs169182901,2
    		C,F--113185361(-) AAAGA-/TGTT  
                TGTTT     		1 -- ut311Minor allele frequency- TGTT:0.01NS 176
    rs155271,2
    		C,--113185477(-) CTATAC/TTGAAA 1 -- ut313Minor allele frequency- T:0.00MN NA 188
    rs799225261,2
    		C,F,--113185725(+) AGTGTT/GTGTTT 1 -- ut311Minor allele frequency- G:0.04WA 118
    rs168888861,2
    		C,--113186517(-) ACCCCC/-ATCCC 1 -- int11Minor allele frequency- -:0.01NS 178

    HapMap Linkage Disequilibrium report for RAD21 (117858173 - 117887105 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for RAD21: --
    Human Gene Mutation Database (HGMD): RAD21

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAD21
    DNA2.0 Custom Variant and Variant Library Synthesis for RAD21

    Disorders / Diseases
    for RAD21 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    RAD21 for disorders           About GeneDecksing

    OMIM gene information: 606462    OMIM disorders: --

    UniProtKB/Swiss-Prot: RAD21_HUMAN, O60216
  • Defects in RAD21 are the cause of Cornelia de Lange syndrome type 4 (CDLS4) [MIM:614701]. A form of Cornelia
    • de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple
    systems. It is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation,
    hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies

    20/21 diseases for RAD21 (see all 21):    About MalaCards
    langer-giedion syndrome    cornelia de lange syndrome    oral squamous cell carcinoma    roberts syndrome
    squamous cell carcinoma    chronic lymphocytic leukemia    fanconi's anemia    lymphocytic leukemia
    kaposi's sarcoma    embryonal carcinoma    anemia    sarcoma
    pancreatic cancer    leukemia    carcinoma    prostate cancer
    breast cancer    pancreatitis    hypoxia    prostatitis

    2 diseases from the University of Copenhagen DISEASES database for RAD21:
    Cornelia de Lange syndrome     Roberts syndrome

    1 Novoseek disease relationship for RAD21 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 0 3 11483345 (2), 15767545 (1)

    Human Genome Epidemiology (HuGE) Navigator: RAD21 (4 documents)

    Export disorders for RAD21 gene to outside databases

    Publications
    for RAD21 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RAD21 gene, integrated from 9 sources (see all 99):
    (articles sorted by number of sources associating them with RAD21)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse. (PubMed id 8812457)1, 2, 3 McKay M.J.... Hoeijmakers J.H.J. (1996)
    2. Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres. (PubMed id 11073952)1, 2, 9 Hoque M.T. and Ishikawa F. (2001)
    3. Novel DNA sequence variants in the hHR21 DNA repair gene in radiosensitive cancer patients. (PubMed id 11483345)1, 2, 9 Severin D.M.... McKay M. (2001)
    4. Cohesin cleavage by separase required for anaphase and cytokinesis in human cells. (PubMed id 11509732)1, 2, 9 Hauf S.... Peters J.-M. (2001)
    5. RAD21 mutations cause a human cohesinopathy. (PubMed id 22633399)1, 2 Deardorff M.A....Kaiser F.J. (2012)
    6. Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates. (PubMed id 15837422)1, 2 Rankin S.... Kirschner M.W. (2005)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    9. Caspase proteolysis of the cohesin component RAD21 promotes apoptosis. (PubMed id 11875078)1, 2 Chen F....Cryns V.L. (2002)
    10. Linking sister chromatid cohesion and apoptosis: role of rad21. (PubMed id 12417729)1, 2 Pati D.... Plon S.E. (2002)

    External Searches for RAD21 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing RAD21 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 5885 HGNC: 9811 AceView: RAD21 Ensembl:ENSG00000164754 euGenes: HUgn5885
    ECgene: RAD21 Kegg: 5885 H-InvDB: RAD21

    Other Databases showing RAD21 gene

    (According to HUGE)
    About This Section
    HUGE: KIAA0078

    Specialized Databases showing RAD21 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RAD21 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for RAD21 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/rad21/

    Intellectual Property
    for RAD21 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for RAD21 gene:
    Search GeneIP for patents involving RAD21

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
    for RAD21 gene

    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     Browse Small Molecules at EMD Millipore
     Browse for Gene Knock-down Tools from EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of RAD21
     Browse Kits and Assays available from EMD Millipore
     Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Phosphatase Activity Assays/Reagents   Browse Recombinant/Natural Proteins  
     OriGene Antibodies for RAD21   OriGene shRNA RFP for RAD21  
     OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAD21   OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for RAD21  
     OriGene Protein Over-expression Lysate for RAD21   Browse OriGene Fluorogenic Cell Assay Kits  
     OriGene siRNA for RAD21   OriGene 3'-UTR Clone for RAD21  
     OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for RAD21   OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for RAD21  
     Browse OriGene GFP tagged cDNA clones in CMV expression vector   Browse OriGene MicroRNA Expression Plasmids  
     Browse OriGene basic RS shRNAs   Browse OriGene validated miRNA SYBR primer pairs  
     Browse OriGene full length recombinant human proteins expressed in human HEK293 cells   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for RAD21   OriGene Custom Protein Services for RAD21  
     OriGene Custom Immunoassay Development  

         		 
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat RAD21
     QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAD21
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAD21
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat RAD21
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat RAD21
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAD21
     GenScript Custom Purified and Recombinant Proteins Services for RAD21 GenScript cDNA clones with any tag delivered in your preferred vector for RAD21
     GenScript Custom Assay Services for RAD21 GenScript Custom Superior Antibodies Services for RAD21
     GenScript Custom overexpressing Cell Line Services for RAD21 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Antibodies & Assays for RAD21 

     Regulatory tfbs in RAD21 promoter
     Search Chromatin IP Primers for RAD21
     RT2 qPCR Primer Assay in human, mouse, rat RAD21
     Search GNC Networks for RAD21
     SABiosciences PCR Arrays including human, mouse, rat RAD21
     Search Tocris compounds for RAD21
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological cDNA Clones
     Antibodies/Proteins Production Services
     Rabbit Monoclonal Antibody Platform
     Bulk Purchasing
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     RAD21 antibodies
     RAD21 proteins
     RAD21 lysates
     Antibodies for RAD21
     See all of Abcam's Antibodies, Kits and Proteins for RAD21
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     RAD21 Proteins, Antibodies, CLIAs, and ELISAs
     Search LifeMap BioReagents cell lines for RAD21
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAD21
     SwitchGear 3'UTR luciferase reporter plasmids for RAD21
     SwitchGear Promoter luciferase reporter plasmids for RAD21
     ThermoFisher Antibodies for RAD21
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat RAD21
           
    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

    View Random Gene

    Category
    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 

    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Copyright © 1996-2013 , Weizmann Institute of Science. All Rights Reserved.
    Hot genes      Disease genes      RAD21 gene at Home site.
    hostname: 356977-web1.xennexinc.com index build: 100 solr: 1.4