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Aliases for RAD18 Gene

Aliases for RAD18 Gene

  • RAD18, E3 Ubiquitin Protein Ligase 2 3 5
  • Postreplication Repair Protein RAD18 3 4
  • RING Finger Protein 73 3 4
  • HRAD18 3 4
  • RNF73 3 4
  • HHR18 3 4
  • Postreplication Repair Protein HRAD18p 3
  • E3 Ubiquitin-Protein Ligase RAD18 3
  • RAD18 Homolog (S. Cerevisiae) 2
  • RAD18, S. Cerevisiae, Homolog 3
  • RAD18 Homolog 3
  • EC 6.3.2.- 4

External Ids for RAD18 Gene

Previous GeneCards Identifiers for RAD18 Gene

  • GC03M008896

Summaries for RAD18 Gene

Entrez Gene Summary for RAD18 Gene

  • The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

GeneCards Summary for RAD18 Gene

RAD18 (RAD18, E3 Ubiquitin Protein Ligase) is a Protein Coding gene. Diseases associated with RAD18 include Congenital Hypoplastic Anemia and Fanconi Anemia, Complementation Group A. Among its related pathways are Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template and Telomere C-strand (Lagging Strand) Synthesis. GO annotations related to this gene include ligase activity and damaged DNA binding.

UniProtKB/Swiss-Prot for RAD18 Gene

  • E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on Lys-164. Has ssDNA binding activity.

Gene Wiki entry for RAD18 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RAD18 Gene

Genomics for RAD18 Gene

Regulatory Elements for RAD18 Gene

Enhancers for RAD18 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH03F009896 1.4 Ensembl ENCODE 75.8 -936.4 -936435 8.2 HDGF PKNOX1 ZFP64 ARID4B SIN3A DMAP1 YBX1 YY1 SLC30A9 CBX5 RAD18 THUMPD3-AS1 IL17RC GHRLOS JAGN1 ENSG00000269982 CRELD1 PRRT3-AS1 CYCSP10 FANCD2
GH03F009889 1.2 ENCODE 43.8 -927.0 -927045 2.4 HDGF PKNOX1 CREB3L1 WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 RAD18 GHRLOS FANCD2 JAGN1 PRRT3 LINC00852 THUMPD3-AS1 IL17RC GC03P009918
GH03F009362 1.2 ENCODE 30.7 -399.4 -399430 2.2 HDGF PKNOX1 ARNT CREB3L1 MLX ARID4B SIN3A FEZF1 DMAP1 ZNF2 RAD18 THUMPD3-AS1 SRGAP3 FANCD2 LINC00852 THUMPD3 GHRLOS SETD5 PRRT3-AS1 ENSG00000254485
GH03F009730 1.2 ENCODE 27.4 -768.3 -768261 3.0 HDGF PKNOX1 ARNT CREB3L1 WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 SRGAP3-AS3 SRGAP3-AS1 RAD18 EMC3-AS1 ENSG00000269982 CYCSP10 ATP2B2-IT1 GHRLOS FANCD2 TATDN2
GH03F009883 1.5 FANTOM5 Ensembl ENCODE 17.2 -920.6 -920619 2.2 PKNOX1 GTF3C2 GATA2 FOS ZNF263 JUNB SMARCB1 SMARCA4 PBX2 STAT3 CIDEC IL17RC JAGN1 RAD18 EMC3-AS1 GHRLOS ENSG00000269982 FANCD2 TATDN2 LINC00852
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around RAD18 on UCSC Golden Path with GeneCards custom track

Promoters for RAD18 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001356841 473 1801 CREB3L1 ARID4B SIN3A ZNF2 YY1 ZNF207 ZNF143 KLF13 ZNF263 SP3

Genomic Location for RAD18 Gene

Chromosome:
3
Start:
8,775,402 bp from pter
End:
8,963,773 bp from pter
Size:
188,372 bases
Orientation:
Minus strand

Genomic View for RAD18 Gene

Genes around RAD18 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RAD18 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RAD18 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RAD18 Gene

Proteins for RAD18 Gene

  • Protein details for RAD18 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NS91-RAD18_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RAD18
    Protein Accession:
    Q9NS91
    Secondary Accessions:
    • Q58F55
    • Q9NRT6

    Protein attributes for RAD18 Gene

    Size:
    495 amino acids
    Molecular mass:
    56223 Da
    Quaternary structure:
    • Homodimer (PubMed:21549715). Interacts with UBE2A and UBE2B, one homodimer binding one molecule of UBE2B. Interacts with SHPRH (PubMed:17130289, PubMed:17108083). Interacts with HLTF (PubMed:18316726, PubMed:18719106). Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (PubMed:22681887). Interacts (via C-terminus and phosphorylated form) with SLF1 (via BRCT domains); this interaction is required for efficient repair of UV-induced DNA damage (PubMed:15632077, PubMed:22036607, PubMed:25931565). Interacts with SLF2 (PubMed:25931565). Interacts with SMC5; this interaction is increased in a SLF1 or SLF2-dependent manner (PubMed:25931565).

    Three dimensional structures from OCA and Proteopedia for RAD18 Gene

neXtProt entry for RAD18 Gene

Post-translational modifications for RAD18 Gene

  • Ubiquitination at Lys 83, Lys 110, Lys 115, Lys 151, Lys 161, Lys 186, Lys 197, Lys 218, Lys 245, Lys 261, Lys 271, Lys 318, Lys 328, and Lys 370
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for RAD18 (RAD18)

No data available for DME Specific Peptides for RAD18 Gene

Domains & Families for RAD18 Gene

Gene Families for RAD18 Gene

Suggested Antigen Peptide Sequences for RAD18 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NS91

UniProtKB/Swiss-Prot:

RAD18_HUMAN :
  • Contains 1 SAP domain.
  • Belongs to the RAD18 family.
  • Contains 1 RING-type zinc finger.
Domain:
  • Contains 1 SAP domain.
Family:
  • Belongs to the RAD18 family.
Similarity:
  • Contains 1 RING-type zinc finger.
  • Contains 1 UBZ-type zinc finger.
genes like me logo Genes that share domains with RAD18: view

Function for RAD18 Gene

Molecular function for RAD18 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on Lys-164. Has ssDNA binding activity.

Enzyme Numbers (IUBMB) for RAD18 Gene

Gene Ontology (GO) - Molecular Function for RAD18 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000403 Y-form DNA binding IDA 18363965
GO:0003677 DNA binding IEA --
GO:0003684 damaged DNA binding NAS 10884424
GO:0005515 protein binding IPI 19549727
GO:0008270 zinc ion binding IEA --
genes like me logo Genes that share ontologies with RAD18: view
genes like me logo Genes that share phenotypes with RAD18: view

Animal Models for RAD18 Gene

MGI Knock Outs for RAD18:

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RAD18 Gene

Localization for RAD18 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RAD18 Gene

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565). {ECO:0000269 PubMed:15632077, ECO:0000269 PubMed:22036607, ECO:0000269 PubMed:25931565}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RAD18 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 3

Gene Ontology (GO) - Cellular Components for RAD18 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus NAS 10884424
GO:0005654 nucleoplasm TAS --
GO:0005657 replication fork IDA 18363965
GO:0005737 cytoplasm IEA --
GO:0005813 centrosome IDA 15632077
genes like me logo Genes that share ontologies with RAD18: view

Pathways & Interactions for RAD18 Gene

genes like me logo Genes that share pathways with RAD18: view

Pathways by source for RAD18 Gene

1 Cell Signaling Technology pathway for RAD18 Gene

UniProtKB/Swiss-Prot Q9NS91-RAD18_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

SIGNOR curated interactions for RAD18 Gene

Activates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for RAD18 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006281 DNA repair IEA,NAS 10884424
GO:0006301 postreplication repair IBA --
GO:0006513 protein monoubiquitination IBA --
GO:0006974 cellular response to DNA damage stimulus IEA,IDA 22036607
GO:0009411 response to UV IBA --
genes like me logo Genes that share ontologies with RAD18: view

Drugs & Compounds for RAD18 Gene

(3) Drugs for RAD18 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved Nutra 0
Adenosine triphosphate Approved Nutra 0

(2) Additional Compounds for RAD18 Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
14000-31-8
genes like me logo Genes that share compounds with RAD18: view

Transcripts for RAD18 Gene

Unigene Clusters for RAD18 Gene

RAD18 homolog (S. cerevisiae):
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RAD18 Gene

No ASD Table

Relevant External Links for RAD18 Gene

GeneLoc Exon Structure for
RAD18
ECgene alternative splicing isoforms for
RAD18

Expression for RAD18 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RAD18 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for RAD18 Gene

This gene is overexpressed in Plasma (45.9) and Cerebrospinal fluid (19.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for RAD18 Gene



Protein tissue co-expression partners for RAD18 Gene

NURSA nuclear receptor signaling pathways regulating expression of RAD18 Gene:

RAD18

SOURCE GeneReport for Unigene cluster for RAD18 Gene:

Hs.375684
genes like me logo Genes that share expression patterns with RAD18: view

Primer Products

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RAD18 Gene

Orthologs for RAD18 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RAD18 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RAD18 34 35
  • 99.12 (n)
dog
(Canis familiaris)
Mammalia RAD18 34 35
  • 88.93 (n)
cow
(Bos Taurus)
Mammalia RAD18 34 35
  • 87.4 (n)
rat
(Rattus norvegicus)
Mammalia Rad18 34
  • 83.37 (n)
mouse
(Mus musculus)
Mammalia Rad18 34 16 35
  • 80.43 (n)
oppossum
(Monodelphis domestica)
Mammalia RAD18 35
  • 57 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RAD18 35
  • 40 (a)
OneToOne
chicken
(Gallus gallus)
Aves RAD18 34 35
  • 64.03 (n)
lizard
(Anolis carolinensis)
Reptilia RAD18 35
  • 72 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rad18 34
  • 62.84 (n)
Str.19594 34
zebrafish
(Danio rerio)
Actinopterygii rad18 34 35
  • 53.89 (n)
zgc55577 34
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RAD18 35 37
  • 22 (a)
OneToOne
Species where no ortholog for RAD18 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for RAD18 Gene

ENSEMBL:
Gene Tree for RAD18 (if available)
TreeFam:
Gene Tree for RAD18 (if available)

Paralogs for RAD18 Gene

genes like me logo Genes that share paralogs with RAD18: view

No data available for Paralogs for RAD18 Gene

Variants for RAD18 Gene

Sequence variations from dbSNP and Humsavar for RAD18 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type
rs10428202 -- 8,909,805(+) gccac(A/G)cataa intron-variant
rs10510395 -- 8,947,468(+) CAGTG(A/C)ATCAG intron-variant
rs1052948 -- 8,880,822(-) TTGCC(C/T)GAGGT utr-variant-3-prime
rs111227421 -- 8,970,433(+) AAAAG(-/A)AAAAA upstream-variant-2KB
rs111229427 -- 8,958,723(+) AGGAA(C/T)GAGGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RAD18 Gene

Variant ID Type Subtype PubMed ID
esv275011 CNV gain+loss 21479260
esv3568605 CNV loss 25503493
nsv518352 CNV loss 19592680
nsv520776 CNV gain 19592680
nsv528867 CNV loss 19592680

Variation tolerance for RAD18 Gene

Residual Variation Intolerance Score: 31.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.10; 51.07% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RAD18 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RAD18

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RAD18 Gene

Disorders for RAD18 Gene

MalaCards: The human disease database

(2) MalaCards diseases for RAD18 Gene - From: DISEASES

Disorder Aliases PubMed IDs
congenital hypoplastic anemia
  • congenital aplastic anemia
fanconi anemia, complementation group a
  • fanconi's anemia
- elite association - COSMIC cancer census association via MalaCards
Search RAD18 in MalaCards View complete list of genes associated with diseases

Relevant External Links for RAD18

Genetic Association Database (GAD)
RAD18
Human Genome Epidemiology (HuGE) Navigator
RAD18
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RAD18
genes like me logo Genes that share disorders with RAD18: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RAD18 Gene

Publications for RAD18 Gene

  1. Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens. (PMID: 10884424) Tateishi S. … Yamaizumi M. (Proc. Natl. Acad. Sci. U.S.A. 2000) 2 3 4 22 64
  2. The human RAD18 gene product interacts with HHR6A and HHR6B. (PMID: 10908344) Xin H. … Wang Z. (Nucleic Acids Res. 2000) 2 3 4 22 64
  3. The association between RAD18 Arg302Gln polymorphism and the risk of human non-small-cell lung cancer. (PMID: 17624554) Kanzaki H. … Shimizu K. (J. Cancer Res. Clin. Oncol. 2008) 3 22 46 64
  4. Single nucleotide polymorphism in the RAD18 gene and risk of colorectal cancer in the Japanese population. (PMID: 17914568) Kanzaki H. … Shimizu K. (Oncol. Rep. 2007) 3 22 46 64
  5. DNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links. (PMID: 25931565) RAoschle M. … Mann M. (Science 2015) 3 4 64

Products for RAD18 Gene

Sources for RAD18 Gene

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