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Aliases for RACGAP1 Gene

Aliases for RACGAP1 Gene

  • Rac GTPase Activating Protein 1 2 3 5
  • Male Germ Cell RacGap 3 4
  • Protein CYK4 Homolog 3 4
  • MgcRacGAP 3 4
  • HsCYK-4 3 4
  • CYK4 3 4
  • KIAA1478 4
  • ID-GAP 3

External Ids for RACGAP1 Gene

Previous GeneCards Identifiers for RACGAP1 Gene

  • GC12M050677
  • GC12M050438
  • GC12M050099
  • GC12M048669
  • GC12M050370

Summaries for RACGAP1 Gene

Entrez Gene Summary for RACGAP1 Gene

  • This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

GeneCards Summary for RACGAP1 Gene

RACGAP1 (Rac GTPase Activating Protein 1) is a Protein Coding gene. Among its related pathways are Platelet activation, signaling and aggregation and Signaling by GPCR. GO annotations related to this gene include protein kinase binding and microtubule binding. An important paralog of this gene is ABR.

UniProtKB/Swiss-Prot for RACGAP1 Gene

  • Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.

Gene Wiki entry for RACGAP1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RACGAP1 Gene

Genomics for RACGAP1 Gene

Regulatory Elements for RACGAP1 Gene

Promoters for RACGAP1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around RACGAP1 on UCSC Golden Path with GeneCards custom track

Genomic Location for RACGAP1 Gene

Chromosome:
12
Start:
49,976,923 bp from pter
End:
50,033,136 bp from pter
Size:
56,214 bases
Orientation:
Minus strand

Genomic View for RACGAP1 Gene

Genes around RACGAP1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RACGAP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RACGAP1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RACGAP1 Gene

Proteins for RACGAP1 Gene

  • Protein details for RACGAP1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H0H5-RGAP1_HUMAN
    Recommended name:
    Rac GTPase-activating protein 1
    Protein Accession:
    Q9H0H5
    Secondary Accessions:
    • Q6PJ26
    • Q9NWN2
    • Q9P250
    • Q9P2W2

    Protein attributes for RACGAP1 Gene

    Size:
    632 amino acids
    Molecular mass:
    71027 Da
    Quaternary structure:
    • Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Associates with alpha-, beta- and gamma-tubulin and microtubules. Interacts via its Rho-GAP domain with RND2. Associates with AURKB during M phase. Interacts via its Rho-GAP domain and basic region with PRC1. The interaction with PRC1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts with SLC26A8 via its N-terminus. Interacts with RAB11FIP3. Interacts with ECT2; the interaction is direct, occurs at anaphase and during cytokinesis in a microtubule-dependent manner and is enhanced by phosphorylation by PLK1. Interacts with KIF23; the interaction is direct.
    SequenceCaution:
    • Sequence=AAH24144.1; Type=Frameshift; Positions=171, 205, 437; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RACGAP1 Gene

neXtProt entry for RACGAP1 Gene

Proteomics data for RACGAP1 Gene at MOPED

Post-translational modifications for RACGAP1 Gene

  • Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • Ubiquitination at Lys 173, Lys 248, Lys 292, Lys 430, Lys 490, and Lys 587
  • Modification sites at PhosphoSitePlus

Antibody Products

No data available for DME Specific Peptides for RACGAP1 Gene

Domains & Families for RACGAP1 Gene

Protein Domains for RACGAP1 Gene

Suggested Antigen Peptide Sequences for RACGAP1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9H0H5

UniProtKB/Swiss-Prot:

RGAP1_HUMAN :
  • The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • Contains 1 phorbol-ester/DAG-type zinc finger.
Domain:
  • The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • The phorbol-ester/DAG-type zinc finger domain mediates interaction with membranes enriched in phosphatidylinositol 3,4,5-trisphosphate and is required during mitotic cytokinesis for normal attachment of the midbody to the cell membrane.
  • Contains 1 Rho-GAP domain.
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.
genes like me logo Genes that share domains with RACGAP1: view

No data available for Gene Families for RACGAP1 Gene

Function for RACGAP1 Gene

Molecular function for RACGAP1 Gene

UniProtKB/Swiss-Prot Function:
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
UniProtKB/Swiss-Prot Induction:
Expression is down-regulated during macrophage differention of HL-60 cells.

Gene Ontology (GO) - Molecular Function for RACGAP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008017 microtubule binding IDA 11782313
GO:0019901 protein kinase binding IPI 19468302
GO:0043015 gamma-tubulin binding IDA 11085985
genes like me logo Genes that share ontologies with RACGAP1: view
genes like me logo Genes that share phenotypes with RACGAP1: view

Animal Model Products

miRNA for RACGAP1 Gene

miRTarBase miRNAs that target RACGAP1

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RACGAP1 Gene

Localization for RACGAP1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RACGAP1 Gene

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RACGAP1 Gene COMPARTMENTS Subcellular localization image for RACGAP1 gene
Compartment Confidence
cytoskeleton 5
cytosol 5
extracellular 5
nucleus 5
plasma membrane 5

Gene Ontology (GO) - Cellular Components for RACGAP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 11085985
GO:0005829 cytosol TAS --
GO:0070062 extracellular exosome IDA 19199708
GO:0072686 mitotic spindle IMP 15642749
genes like me logo Genes that share ontologies with RACGAP1: view

Pathways & Interactions for RACGAP1 Gene

genes like me logo Genes that share pathways with RACGAP1: view

SIGNOR curated interactions for RACGAP1 Gene

Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for RACGAP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000281 mitotic cytokinesis IMP 16236794
GO:0000915 actomyosin contractile ring assembly IMP 16129829
GO:0007264 small GTPase mediated signal transduction TAS --
GO:0007405 neuroblast proliferation ISS --
GO:0009790 embryo development ISS --
genes like me logo Genes that share ontologies with RACGAP1: view

Drugs & Compounds for RACGAP1 Gene

(3) Additional Compounds for RACGAP1 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)
genes like me logo Genes that share compounds with RACGAP1: view

Transcripts for RACGAP1 Gene

Unigene Clusters for RACGAP1 Gene

Rac GTPase activating protein 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RACGAP1 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c · 9d ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^
SP1: - - - - -
SP2: - - -
SP3: - - - -
SP4: - - - -
SP5: - - - - -
SP6: -
SP7: - - - -
SP8: - - - -
SP9: - - - -
SP10: - - -
SP11: - - - - - -
SP12: - - - -
SP13: - - -
SP14: - - -
SP15: - - - -
SP16: - - - -
SP17: - - -
SP18: -
SP19: - - - - - - - -

ExUns: 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:
SP18:
SP19:

Relevant External Links for RACGAP1 Gene

GeneLoc Exon Structure for
RACGAP1
ECgene alternative splicing isoforms for
RACGAP1

Expression for RACGAP1 Gene

mRNA expression in normal human tissues for RACGAP1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RACGAP1 Gene

This gene is overexpressed in Testis (x4.1).

Protein differential expression in normal tissues from HIPED for RACGAP1 Gene

This gene is overexpressed in Spinal cord (23.6), Lung (17.2), Testis (12.3), and Esophagus (8.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for RACGAP1 Gene



SOURCE GeneReport for Unigene cluster for RACGAP1 Gene Hs.505469

mRNA Expression by UniProt/SwissProt for RACGAP1 Gene

Q9H0H5-RGAP1_HUMAN
Tissue specificity: Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
genes like me logo Genes that share expression patterns with RACGAP1: view

Primer Products

In Situ Assay Products

No data available for Protein tissue co-expression partners for RACGAP1 Gene

Orthologs for RACGAP1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RACGAP1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RACGAP1 35
  • 91.46 (n)
  • 95.25 (a)
RACGAP1 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RACGAP1 35
  • 92.93 (n)
  • 96.04 (a)
RACGAP1 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Racgap1 35
  • 84.26 (n)
  • 85.17 (a)
Racgap1 16
Racgap1 36
  • 85 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RACGAP1 35
  • 99.63 (n)
  • 99.53 (a)
RACGAP1 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Racgap1 35
  • 84.43 (n)
  • 85.92 (a)
oppossum
(Monodelphis domestica)
Mammalia RACGAP1 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RACGAP1 36
  • 88 (a)
OneToOne
chicken
(Gallus gallus)
Aves RACGAP1 35
  • 74.87 (n)
  • 78.73 (a)
RACGAP1 36
  • 66 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RACGAP1 36
  • 81 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia racgap1 35
  • 70.75 (n)
  • 76.27 (a)
Str.360 35
African clawed frog
(Xenopus laevis)
Amphibia MGC53048 35
zebrafish
(Danio rerio)
Actinopterygii racgap1 35
  • 66.51 (n)
  • 67.83 (a)
wufb92c08 35
racgap1 36
  • 65 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.12503 35
fruit fly
(Drosophila melanogaster)
Insecta RacGAP50C 37
  • 33 (a)
RacGAP84C 37
  • 41 (a)
tum 35
  • 48.06 (n)
  • 39.33 (a)
RacGAP84C 36
  • 36 (a)
OneToMany
RhoGAP16F 36
  • 15 (a)
OneToMany
RhoGAP18B 36
  • 9 (a)
OneToMany
tum 36
  • 34 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008912 35
  • 45.47 (n)
  • 36.21 (a)
worm
(Caenorhabditis elegans)
Secernentea cyk-4 37
  • 32 (a)
cyk-4 36
  • 21 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes BEM2 36
  • 5 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8791 36
  • 44 (a)
OneToOne
Species with no ortholog for RACGAP1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RACGAP1 Gene

ENSEMBL:
Gene Tree for RACGAP1 (if available)
TreeFam:
Gene Tree for RACGAP1 (if available)

Paralogs for RACGAP1 Gene

Paralogs for RACGAP1 Gene

Pseudogenes.org Pseudogenes for RACGAP1 Gene

genes like me logo Genes that share paralogs with RACGAP1: view

Variants for RACGAP1 Gene

Sequence variations from dbSNP and Humsavar for RACGAP1 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs8987 -- 49,989,367(+) GCAAA(A/G)AAAAA utr-variant-3-prime
rs15192 -- 49,989,542(+) TAAAA(A/G)AAAAA utr-variant-3-prime
rs598144 -- 50,008,784(-) CGGGC(A/G)TGGTG intron-variant
rs694851 -- 50,032,588(-) ACCCG(C/T)GCCCC intron-variant, upstream-variant-2KB
rs602772 -- 50,005,130(+) TCCCC(A/G)CAAAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RACGAP1 Gene

Variant ID Type Subtype PubMed ID
nsv522972 CNV Gain 19592680
nsv832407 CNV Loss 17160897
esv271791 CNV Insertion 20981092
esv1766563 CNV Insertion 17803354
esv1010880 CNV Insertion 20482838

Variation tolerance for RACGAP1 Gene

Residual Variation Intolerance Score: 49.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.43; 28.08% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for RACGAP1 Gene

HapMap Linkage Disequilibrium report
RACGAP1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RACGAP1 Gene

Disorders for RACGAP1 Gene

Relevant External Links for RACGAP1

Genetic Association Database (GAD)
RACGAP1
Human Genome Epidemiology (HuGE) Navigator
RACGAP1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
RACGAP1

No disorders were found for RACGAP1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RACGAP1 Gene

Publications for RACGAP1 Gene

  1. MgcRacGAP, a new human GTPase-activating protein for Rac and Cdc42 similar to Drosophila rotundRacGAP gene product, is expressed in male germ cells. (PMID: 9497316) Toure A. … Gacon G. (J. Biol. Chem. 1998) 2 3 4 23 67
  2. Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation. (PMID: 19468300) Wolfe B.A. … Glotzer M. (PLoS Biol. 2009) 3 23
  3. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. (PMID: 19468302) Burkard M.E. … Jallepalli P.V. (PLoS Biol. 2009) 3 23
  4. CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase. (PMID: 19015243) Seguin L. … Bertoglio J. (Mol. Cell. Biol. 2009) 3 23
  5. Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase. (PMID: 18201571) TourAc A. … Bertoglio J. (FEBS Lett. 2008) 3 23

Products for RACGAP1 Gene

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