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RAB4B Gene

protein-coding   GIFtS: 53

GC19P045979
RAB4B, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ78649 1, 2
MGC52123 1, 2
Descriptions
RAB4B, member RAS oncogene family 2
ras-related GTP-binding protein 4b 2
small GTP binding protein RAB4B 2
External Ids
HGNC: 97821
Entrez Gene: 539162
UniProtKB: P610183
Ensembl: ENSG000001675787
Search outside databases for aliases for RAB4B gene

Previous GC identifers: GC19P041923 GC19P041675 GC19P045960 GC19P045974 GC19P045977

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: RAB4B_HUMAN, P61018
Function: Protein transport. Probably involved in vesicular traffic (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB4B gene  

Entrez Gene cytogenetic band: 19q13.2   Ensembl cytogenetic band:  19q13.2   HGNC cytogenetic band: 19q13.2

RAB4B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P045979:     (about GC identifiers)

Start:
45,976,011 bp from pter
End:
45,994,687 bp from pter
Size:
18,677 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011109.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: RAB4B_HUMAN, P61018 (See protein sequence)
Recommended Name: Ras-related protein Rab-4B  
Size: 213 amino acids; 23587 Da
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential)
PDB structures from and Proteopedia :
2O52 (3D)    
Secondary accessions: P22750 Q7Z514 Q9HBR6
Alternative splicing: 2 isoforms:  P61018-1   P61018-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_057238.3  

    ENSEMBL proteins: 
    ENSP00000367557 ENSP00000349560 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for RAB4B  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (RAB4B)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular NAS--
    GO:0005886 plasma membrane IEA--
    About this table

    Antibodies for RAB4B: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for RAB4B

    Assays for RAB4B: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB4B for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P61018

    ProtoNet protein and cluster: P61018

    4 Blocks protein families:
    IPB001806 Transforming protein P21 RAS signature
    IPB002041 GTP-binding nuclear protein Ran
    IPB003577 Ras small GTPase
    IPB003578 Ras small GTPase


    UniProtKB/Swiss-Prot: RAB4B_HUMAN, P61018
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB4B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB4B)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016154

                  Applied Biosystems Silencer® siRNAs for RAB4B

                  Sigma-Aldrich siRNA for RAB4B  
                         Sigma-Aldrich shRNA for RAB4B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for RAB4B
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_016154
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_016154
                                     untagged cDNA clone in CMV expression vector: NM_016154 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_016154

    UniProtKB/Swiss-Prot: RAB4B_HUMAN, P61018
    Function: Protein transport. Probably involved in vesicular traffic (By similarity)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003924 GTPase activity NAS--
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for RAB4B
     Clathrin-dependent protein traffic

       GeneDecks  RAB4B for the pathways selected above  
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    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  RAB4B  (Your Favorite Gene powered by Ingenuity) 
     Clathrin-mediated Endocytosis

       GeneDecks  RAB4B for the pathways selected above  
    About GeneDecksing

      --

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0015031 protein transport IEA--
    GO:0016192 vesicle-mediated transport NAS--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB4B
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB4B

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB4B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB4B)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016154

                  Sigma-Aldrich siRNA for RAB4B  
                         Sigma-Aldrich shRNA for RAB4B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_016154  

    REFSEQ mRNAs for RAB4B gene: 

    NM_016154.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_016154  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_016154
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_016154
                                     untagged cDNA clone in CMV expression vector: NM_016154 

    Additional cDNA sequence: 

    AF165522.1 AF217985.1 AF498935.1 AK024845.1 BC033081.1 BC040374.1 BC046927.1 

    14 DOTS entries:

    DT.121503310  DT.97843879  DT.91750613  DT.121503377  DT.211984  DT.121503359  DT.215113  DT.100029100 
    DT.100792663  DT.99954030  DT.95208557  DT.121503405  DT.95164335  DT.121503386 

    24/186 AceView cDNA sequences (see all 186 ):

    BQ686015 AF498935 BU538102 AI086169 CA432161 BC046927 AW136325 BM561937 
    AI493506 AI270301 AA815081 BU956946 AA418199 AI797993 AI830148 BU150091 
    BX280685 BQ957483 AW793349 BM856478 AI934691 AI312115 BE674995 BX480149 

    highest scoring ESTs for RAB4B:

    AW005647 AA243688 AA418027 AA418199 AA461189 AA469897 AA478683 AA496001 AA525114 AA813726 

    Unigene Cluster for RAB4B:

    RAB4B, member RAS oncogene family
    Hs.631539  [show with all ESTs]
    Unigene Representative Sequence: AF217985


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for RAB4B

    ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9
    SP1:        -     -                             -                     
    SP2:                                            -                     
    SP3:                                      -     -                     
    SP4:                                                                  

    About this scheme

    ECgene alternative splicing isoforms for RAB4B

    2 Ensembl transcripts including schematic representations:
    ENST00000378307  ENST00000357052  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB4B expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB4B

    1 / 2 / 3

    8 probe-sets matching RAB4B gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    53177_at2, 3 U95-B 1 1.00 1.00 0.79 1.25 AW005647 1.00 0.90 0.95 1
    39272_g_at2, 3 U95-A 2 1.00 0.75 0.95 0.75 AA461365 0.60 0.50 0.55 1

    233385_x_at2, 3 U133-B 1 1.00 1.00 -- -- AK024845 0.80 0.88 0.84 1

    228521_s_at2, 3 U133-B 1 1.00 1.00 -- -- AW242973 0.80 0.88 0.84 1

    219807_x_at2, 3 U133-A 1 1.00 1.00 -- -- NM_016154 0.60 0.83 0.73 1

    233385_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    228521_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    219807_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  RAB4B for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TCTCCTTCAT

    SOURCE GeneReport for Unigene cluster: Hs.631539

    Expression variation in blood from EXPOLDB for RAB4B

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB4B gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RAB4B1   -- RAB4B, member RAS oncogene family 94.37(n)
    100(a)
    403956  XM_850495.1  XP_855588.1 
    rat
    (Rattus norvegicus)
    Rab4b1   -- RAB4B, member RAS oncogene family 91.86(n)
    99.53(a)
    50866  NM_017355.1  NP_059051.1 
    mouse
    (Mus musculus)
    Rab4b1, 5 7 (7.40 cM)5
    RAB4B, member RAS oncogene family1, 5 91.55(n)1
    99.53(a)1
    193421  NM_029391.21  NP_083667.11 
     AB2325925  AF4084325  (see all 11)
    zebrafish
    (Danio rerio)
    zgc:1010151   -- zgc:101015 79.03(n)
    91.08(a)
    445498  NM_001004002.1  NP_001004002.1 
    fruit fly
    (Drosophila melanogaster)
    Rab41, 3 54C9--103
    RAB small monomeric GTPase3
    Rab-protein 41
    75(a)
    (best of 2)3
    67.77(n)1
    76.78(a)1
    369921  NM_079053.21  NP_523777.11 
    About this table        Species with no ortholog for RAB4B

    ENSEMBL Gene Tree for RAB4B
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB4B gene
    RAB2A2  RAB142  RAB4A2  RAB2B2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/126 NCBI SNPs in RAB4B are shown (see all 126 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 62)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs79371,2
    A,C,F,H,O45994546(+) CCATAT/CCCCAA 1 -- ut31 ese314Minor allele frequency- C:0.42MN EA NA EU WA 2842
    rs22576131,2
    A,C45975844(-) GGCGGA/GGCCGA 1 -- ng510--------
    --
    rs26448991,2
    A,C45994789(+) TTTTTG/TTTGTT 1 -- ng310--------
    rs123276421,2
    H45994731(+) GAAGAG/TGTCCC 1 -- ng314Minor allele frequency- T:0.00EU EA WA 418
    rs355730021,2
    F45984441(+) CTCACA/TGGCGA 1 T/T syn11Minor allele frequency- T:0.08NA 78
    rs72522271,2
    A,C,F,H45992955(+) cagttT/Gcagga 1 -- int18Minor allele frequency- G:0.42EU EA WA 828
    rs22796991,2
    A,C,F,H45976238(-) CATCAC/TACCAG 1 -- int16Minor allele frequency- T:0.17EA MN EU WA 2070
    rs26049101,2
    C,F,H,O45989094(-) GGAGCA/GACAGG 1 -- int17Minor allele frequency- N:0.00EA WA NA 482
    rs22876911,2
    C,F,H,O45978003(+) TTCTCC/GTTGAC 1 -- int113Minor allele frequency- N:0.00EA EU WA NA 2548
    rs26448981,2
    C,F,H,O45990855(+) GCCATA/GCTTAT 1 -- int17Minor allele frequency- G:0.21EU EA WA NA 554
    About this table

    HapMap Linkage Disequilibrium images for RAB4B (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    5 PubMed articles for RAB4B gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 53916 HGNC: 9782 AceView: MIAandRAB4B Ensembl:ENSG00000167578 euGenes: HUgn53916
    ECgene: RAB4B H-InvDB: RAB4B
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB4B Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB4B:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Drugs for RAB4B
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     Search Novus for antibodies for RAB4B

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