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RAB4A Gene

protein-coding   GIFtS: 62

GC01P227473
RAB4A, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: RAB4)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
HRES-1/RAB4 1, 2
OTTHUMP00000035740 2
OTTHUMP00000042824 2
RAB4 2, 3, 5
Descriptions
Oncogene RAB4 2
RAB4, member RAS oncogene family 2
RAB4A, member RAS oncogene family 2
External Ids
HGNC: 97811
Entrez Gene: 58672
UniProtKB: P203383
Ensembl: ENSG000001681187
Search outside databases for aliases for RAB4A gene

Previous GC identifers: GC01P227854 GC01P225101 GC01P225807 GC01P226368 GC01P225713

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RAB4A_HUMAN, P20338
Function: Protein transport. Probably involved in vesicular traffic (By similarity)

Gene Wiki entry for RAB4A

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB4A gene  

Entrez Gene cytogenetic band: 1q42-q43   Ensembl cytogenetic band:  1q42.13   HGNC cytogenetic band: 1q42-q43

RAB4A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P227473:     (about GC identifiers)

Start:
227,473,502 bp from pter
End:
227,508,261 bp from pter
Size:
34,760 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_004559.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB4A_HUMAN, P20338 (See protein sequence)
Recommended Name: Ras-related protein Rab-4A  
Size: 213 amino acids; 23871 Da
Subunit: Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1. Interacts with SGSM1, SGSM2 and SGSM3
(By similarity)
Subcellular location: Membrane; Peripheral membrane protein. Cytoplasm. Note=Generally associated
with membranes. Cytoplasmic when phosphorylated by CDC2
PDB structures from and Proteopedia :
1YU9 (3D)    1Z0K (3D)    2BMD (3D)    2BME (3D)    
Secondary accessions: Q5T7P7 Q9BQ44

Post-translational modifications:

  • Phosphorylated by CDC2 kinase during mitosis1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_004569.2  

    ENSEMBL proteins: 
    ENSP00000355651 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for RAB4A  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Rab4)
    Human Recombinant Proteins from Abnova (RAB4A)
                  OriGene Purified Recombinant Human Protein: RAB4A 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0016020 membrane IEA--
    About this table

    Antibodies for RAB4A: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of RAB4A
    Sigma-Aldrich Antibodies for RAB4A
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for RAB4A  (Rab4)
    Antibodies from Abcam (Rab4), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (RAB4A)
    Novus Biologicals Antibodies for RAB4A

    Assays for RAB4A: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB4A for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P20338

    ProtoNet protein and cluster: P20338

    1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

    UniProtKB/Swiss-Prot: RAB4A_HUMAN, P20338
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB4A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB4A)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004578

                  Applied Biosystems Silencer® siRNAs for RAB4A

                  Sigma-Aldrich siRNA for RAB4A  
                         Sigma-Aldrich shRNA for RAB4A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for RAB4A
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004578
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004578
                                     untagged cDNA clone in CMV expression vector: NM_004578 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004578

    UniProtKB/Swiss-Prot: RAB4A_HUMAN, P20338
    Function: Protein transport. Probably involved in vesicular traffic (By similarity)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003924 GTPase activity TAS2501306
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Millipore Pathways for RAB4A
     Clathrin-dependent protein traffic
     Selected targets of ESR1

       GeneDecks  RAB4A for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  RAB4A  (Your Favorite Gene powered by Ingenuity) 
     Clathrin-mediated Endocytosis

       GeneDecks  RAB4A for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  RAB4A 


    5/7 Interacting proteins for RAB4A (P203382) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HGSO149642MINT-3369790
    RAB11AP624912MINT-3369790
    RAB14P611062MINT-3369790
    RAB7AP511492MINT-3369790
    SRA1Q9HD152MINT-63548
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0015031 protein transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB4A
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB4A
    4 Novoseek chemical compound relationships for RAB4A gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    gdp 61.83 8 16389071 (3), 9303294 (1), 16413502 (1), 15292453 (1) (see all 5)
    gtp 54.29 6 16389071 (2), 16413502 (2), 9298810 (1), 8858211 (1)
    sodium 0.00 1 16389071 (1)
    glucose 0.00 9 9182703 (6), 10320056 (1), 8943343 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB4A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB4A)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004578

                  Sigma-Aldrich siRNA for RAB4A  
                         Sigma-Aldrich shRNA for RAB4A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004578  

    REFSEQ mRNAs for RAB4A gene: 

    NM_004578.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004578  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004578
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004578
                                     untagged cDNA clone in CMV expression vector: NM_004578 

    Additional cDNA sequence: 

    AF498934.1 AK223009.1 AK313807.1 AY585832.1 BC002438.1 BC004309.1 CR602754.1 CR604213.1 
    CR604401.1 CR605031.1 CR615332.1 CR617877.1 M28211.1 

    9 DOTS entries:

    DT.452406  DT.95124080  DT.100709424  DT.91763923  DT.100784308  DT.91848512  DT.121429877  DT.75118334 
    DT.95124081 

    24/226 AceView cDNA sequences (see all 226 ):

    AI803193 CB105949 CR602754 CB529964 CB250394 NM_004578 BQ055622 Z44753 
    AI871299 AI394208 CR615332 CR605031 AI031985 AU130796 AI203545 AI479971 
    AA099743 NM_006542 BX358091 CR617877 CR604213 BE208249 BC004309 CR604401 

    highest scoring ESTs for RAB4A:

    AA001687 AA084625 AA099743 AA234586 AA280807 AA280857 AA296757 AA377755 AA381010 AA452226 

    Unigene Cluster for RAB4A:

    RAB4A, member RAS oncogene family
    Hs.296169  [show with all ESTs]
    Unigene Representative Sequence: AY585832


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for RAB4A

    ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c
    SP1:              -                                                               
    SP2:                                                                              
    SP3:                                -                                             
    SP4:        -     -     -           -                                             
    SP5:        -     -     -                                                         

    About this scheme

    ECgene alternative splicing isoforms for RAB4A

    1 Ensembl transcript including schematic representation:
    ENST00000366690  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB4A expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB4A

    1 / 2 / 3

    10 probe-sets matching RAB4A gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    621_at2, 3 U95-A 1 1.00 1.00 0.96 1.01 M28211 0.80 0.88 0.84 1
    39244_at2, 3 U95-A 1 1.00 1.00 0.92 0.93 M28211 0.80 0.88 0.84 1
    1686_g_at2, 3 U95-A 2 1.00 0.50 0.95 1.22 X82554 0.20 0.50 0.38 1

    1685_at2, 3 U95-A 2 1.00 0.50 0.95 0.85 X82554 0.20 0.50 0.38 1

    203581_at2, 3 U133-A 1 1.00 1.00 -- -- BC002438 0.80 0.88 0.84 1

    203582_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004578 0.60 0.83 0.73 1

    206272_at2, 3 U133-A 2 1.00 0.50 -- -- NM_006542 0.60 0.83 0.73 1

    203581_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    203582_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206272_at2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
    GeneDecks  RAB4A for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTGTTTACGT

    SOURCE GeneReport for Unigene cluster: Hs.296169

    Expression variation in blood from EXPOLDB for RAB4A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB4A gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RAB4A1   -- RAB4A, member RAS oncogene family 93.73(n)
    99.54(a)
    479210  XM_536353.2  XP_536353.2 
    cow
    (Bos taurus)
    RAB4A1   -- RAB4A, member RAS oncogene family 90.98(n)
    99.08(a)
    615849  NM_001038685.1  NP_001033774.1 
    rat
    (Rattus norvegicus)
    Rab4a1   -- RAB4A, member RAS oncogene family 86.54(n)
    98.17(a)
    25532  NM_013019.2  NP_037151.2 
    mouse
    (Mus musculus)
    Rab4a1, 5 8 (67.50 cM)5
    RAB4A, member RAS oncogene family1, 5 86.38(n)1
    97.65(a)1
    193411  NM_009003.21  NP_033029.11 
     AB2325915  AI8482685  (see all 13)
    chicken
    (Gallus gallus)
    RAB4A1   -- RAB4A, member RAS oncogene family 85.17(n)
    98.62(a)
    421532  XM_419573.2  XP_419573.1 
    About this table        Species with no ortholog for RAB4A

    ENSEMBL Gene Tree for RAB4A
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB4A gene
    RAB2A2  RAB142  RAB4B2  RAB2B2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/145 NCBI SNPs in RAB4A are shown (see all 145 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 62)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs168417331,2
    F227472951(+) GGCCC-/CGGCAG 1 -- ng512Minor allele frequency- C:0.19MN EA 356
    rs2377491,2
    A,C,F,H,O227478332(-) TCATAA/CTTTAT 1 -- int14Minor allele frequency- C:0.36EU EA WA 416
    rs2377771,2
    A,C,F,H,O227498539(-) AAGAAA/GTGTAA 1 -- int117Minor allele frequency- N:0.00MN EU EA WA NA 1654
    rs4194781,2
    A,C,F,H,O227475618(-) AGATAT/AGCTCT 1 -- int15Minor allele frequency- A:0.38MN EU EA WA 602
    rs2377791,2
    A,C,F,H227501899(-) AGAAAT/CAAAAT 1 -- int18Minor allele frequency- C:0.20EA NA EU WA 638
    rs2377621,2
    A,C,F,O227488525(-) GCAAAG/CTTAGT 1 -- int14Minor allele frequency- C:0.18EA NA 154
    rs1491571,2
    A,C,F,H227491824(-) TCACTG/TAATTA 1 -- int15Minor allele frequency- T:0.23MN EU EA WA 596
    rs2377561,2
    A,C,F,H227486044(-) gtaccA/Cgggat 1 -- int18Minor allele frequency- C:0.33EU EA WA 824
    rs2377711,2
    A,C,F,H227496168(-) TTAACT/ACTATC 1 -- int15Minor allele frequency- A:0.25MN EU EA WA 562
    rs2377481,2
    A,C,F,O227477151(-) AATTTT/CAAAAG 1 -- int13Minor allele frequency- C:0.42NA EA 252
    About this table

    HapMap Linkage Disequilibrium images for RAB4A (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 179511

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/74 PubMed articles for RAB4A gene (see all 74 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5867 HGNC: 9781 AceView: RAB4A Ensembl:ENSG00000168118 euGenes: HUgn5867
    ECgene: RAB4A H-InvDB: RAB4A
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB4A Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB4A:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Lentivirus-delivered shRNAs for RAB4A Browse small molecules at Sigma-Aldrich
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     Antibodies & Assays for RAB4A  (Rab4)

     Recombinant Proteins
    (Rab4)
     Antibodies (Rab4)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for RAB4A




     Drugs for RAB4A
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for RAB4A

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