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RAB3A Gene

protein-coding   GIFtS: 66

GC19M018168
RAB3A, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Descriptions
RAB3A, member RAS oncogene family 2
RAS-associated protein RAB3A 2
External Ids
HGNC: 97771
Entrez Gene: 58642
UniProtKB: P203363
Ensembl: ENSG000001056497
Search outside databases for aliases for RAB3A gene

Previous GC identifers: GC19M018801 GC19M018699 GC19M018152

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: RAB3A_HUMAN, P20336
Function: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a
role in neurotransmitter release by regulating membrane flow in the nerve terminal

Gene Wiki entry for RAB3A

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB3A gene  

Entrez Gene cytogenetic band: 19p13.2   Ensembl cytogenetic band:  19p13.11   HGNC cytogenetic band: 19p13.2

RAB3A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M018168:     (about GC identifiers)

Start:
18,168,611 bp from pter
End:
18,175,839 bp from pter
Size:
7,229 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011295.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB3A_HUMAN, P20336 (See protein sequence)
Recommended Name: Ras-related protein Rab-3A  
Size: 220 amino acids; 24984 Da
Subunit: Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with
RPH3A. Interacts with the exocyst complex through SEC15. Binds SYTL4, RIMS1 and RIMS2. Interacts
with RAB3IP. Interacts with SGSM1 and SGSM3 (By similarity)
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential)
Secondary accessions: Q9NYE1

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_002857.1  

    ENSEMBL proteins: 
    ENSP00000222256 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for RAB3A  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Rab3A, Rab3A + Rab3B + Rab3C + Rab3D)
    Human Recombinant Proteins from Abnova (RAB3A)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IDA18029348
    GO:0005886 plasma membrane IEA--
    GO:0008021 synaptic vesicle TAS9194562
    About this table

    Antibodies for RAB3A: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of RAB3A
    Sigma-Aldrich Antibodies for RAB3A
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Rab3A, Rab3A + Rab3B + Rab3C + Rab3D), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (RAB3A)
    Novus Biologicals Antibodies for RAB3A

    Assays for RAB3A: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR015596 Rab3
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB3A for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P20336

    ProtoNet protein and cluster: P20336

    1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

    UniProtKB/Swiss-Prot: RAB3A_HUMAN, P20336
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB3A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB3A)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002866

                  Applied Biosystems Silencer® siRNAs for RAB3A

                  Sigma-Aldrich siRNA and siRNA Panels for RAB3A  
                         Sigma-Aldrich shRNA for RAB3A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002866
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002866
                                     untagged cDNA clone in CMV expression vector: NM_002866 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_002866

    UniProtKB/Swiss-Prot: RAB3A_HUMAN, P20336
    Function: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a
    role in neurotransmitter release by regulating membrane flow in the nerve terminal

    Genatlas biochemistry entry for RAB3A:
    RAB3A,Ras oncogene superfamily member,expressed synaptic vesicles, involved in calcium regulated
    exocytosis,expressed in parathyroid chief cells

    7 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Rab3a):

    behavior/neurologicalgrowth/sizehomeostasis/metabolismlethality-prenatal/perinatalnervous system
    normalrespiratory system

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003924 GTPase activity IMP10859313
    GO:0005515 protein binding ISS--
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  RAB3A 


    5/45 Interacting proteins for RAB3A (ENSP000002222563) via UniProtKB, MINT, and/or STRING (see all 45 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RIMS1ENSP000002648393STRING (score=.998)
    SNAP25ENSP000002549763STRING (score=.996)
    STXBP1ENSP000003623993STRING (score=.994)
    RAB3IPENSP000003233493STRING (score=.989)
    CHMENSP000003503863STRING (score=.988)
    About this table

    5/14 Gene Ontology (GO) biological process terms (links to tree view) (see all 14 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003016 respiratory system process IEA--
    GO:0007005 mitochondrion organization IEA--
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0007274 neuromuscular synaptic transmission IEA--
    GO:0007409 axonogenesis IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB3A
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB3A
    4 Novoseek chemical compound relationships for RAB3A gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    gtp 66.06 19 8886977 (3), 7926025 (3), 8387493 (2), 9252412 (1) (see all 8)
    gdp 62.83 6 9252412 (1), 7926025 (1), 8188702 (1), 7945346 (1)
    sucrose 5.92 1 8886977 (1)
    calcium 0.00 9 11522668 (3), 10954749 (2), 17625073 (2)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB3A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB3A)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002866

                  Sigma-Aldrich siRNA and siRNA Panels for RAB3A  
                         Sigma-Aldrich shRNA for RAB3A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_002866  

    REFSEQ mRNAs for RAB3A gene: 

    NM_002866.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_002866  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002866
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002866
                                     untagged cDNA clone in CMV expression vector: NM_002866 

    Additional cDNA sequence: 

    AF254795.1 AF498931.1 AK289559.1 AK293467.1 BC011782.2 M28210.1 

    5 DOTS entries:

    DT.444206  DT.100653326  DT.100668937  DT.40110695  DT.95301547 

    24/124 AceView cDNA sequences (see all 124 ):

    AW205498 CA309409 AA364643 BX442130 CD615936 BM926739 BC011782 BI917111 
    BQ898770 BU739142 BX419052 AL534649 AW163583 AW205866 BM673547 AW160374 
    BP360364 NM_002866 BM921884 BI820799 BU944659 BI914074 AL517560 AF498931 

    highest scoring ESTs for RAB3A:

    M28210 AF254795 AI879002 AW961221 BC011782 BE265314 BE791798 BE792476 BG395376 BG819310 

    Unigene Cluster for RAB3A:

    RAB3A, member RAS oncogene family
    Hs.27744  [show with all ESTs]
    Unigene Representative Sequence: NM_002866


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for RAB3A

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b
    SP1:                    -           -                           
    SP2:                    -     -     -                           
    SP3:                                -                           
    SP4:                    -                                       

    About this scheme

    ECgene alternative splicing isoforms for RAB3A

    1 Ensembl transcript including schematic representation:
    ENST00000222256  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB3A expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB3A

    1 / 2 / 3

    4 probe-sets matching RAB3A gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    57558_at2, 3 U95-B 1 1.00 1.00 0.98 1.34 AA988241 0.80 0.80 0.80 1

    620_at2, 3 U95-A 1 1.00 1.00 0.90 0.68 M28210 1.00 1.00 1.00 1

    204974_at2, 3 U133-A 1 1.00 1.00 -- -- AA988241 0.80 0.80 0.80 1

    204974_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  RAB3A for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ACAACACTAC

    SOURCE GeneReport for Unigene cluster: Hs.27744

    Expression variation in blood from EXPOLDB for RAB3A

    UniProtKB/Swiss-Prot: RAB3A_HUMAN, P20336
    Tissue specificity: Specifically expressed in brain

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB3A gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RAB3A1   -- RAB3A, member RAS oncogene family 93.18(n)
    99.09(a)
    484826  XM_541942.2  XP_541942.2 
    chimpanzee
    (Pan troglodytes)
    RAB3A1   -- RAB3A, member RAS oncogene family 100(n)
    100(a)
    455857  XM_001173871.1  XP_001173871.1 
    cow
    (Bos taurus)
    RAB3A1   -- RAB3A, member RAS oncogene family 91.67(n)
    98.64(a)
    282029  NM_174446.3  NP_776871.2 
    rat
    (Rattus norvegicus)
    Rab3a1   -- RAB3A, member RAS oncogene family 88.48(n)
    99.09(a)
    25531  NM_013018.2  NP_037150.2 
    mouse
    (Mus musculus)
    Rab3a1, 5 8 (32.60 cM)5
    RAB3A, member RAS oncogene family1, 5 89.24(n)1
    99.09(a)1
    193391  NM_009001.41  NP_033027.11 
     AB2325875  AK0053075  (see all 11)
    About this table        Species with no ortholog for RAB3A

    ENSEMBL Gene Tree for RAB3A
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB3A gene
    RAB3B2  RAB122  RAB3D2  RAB3C2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/41 NCBI SNPs in RAB3A are shown (see all 41 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 22)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20490501,2
    C,F,H18176629(+) CGCCCC/TGTCAT 1 -- ng3112Minor allele frequency- T:0.21MN EU EA WA 2779
    rs20490511,2
    C,F,H18176831(+) CAGAGA/GATGGA 1 -- ng3112Minor allele frequency- G:0.28MN EU EA WA NA 2688
    rs22803441,2
    A,C,F18176283(+) CTCTTC/GAGGCC 1 -- ng316Minor allele frequency- G:0.33MN EA NA 718
    rs22718831,2
    C,F18175917(+) CGCGCT/GTAGAG 1 -- ng312Minor allele frequency- G:0.35MN EA 356
    --
    rs118819781,2
    C18175814(+) GGCAGA/CAGCGG 1 -- ut51 ese30--------
    --
    rs10465651,2
    C,F,O18172199(-) GGCGCT/CATGGG 1 A/A syn1 ese34Minor allele frequency- C:0.22NA 214
    rs22803421,2
    C,F,H18174515(+) GACTCC/TTTCTG 1 K/K syn1 ese39Minor allele frequency- T:0.15EA EU WA 2328
    rs22718821,2
    A,C,F,H18170365(+) ATGGCA/GAACTC 1 -- int110Minor allele frequency- G:0.44NA EU EA WA 1022
    rs169954851,2
    C,F,H18171678(+) CACTTG/CCAGAT 1 -- int111Minor allele frequency- C:0.02NA EU EA WA 980
    rs81098071,2
    A,C18169508(+) GGCCAC/TGCCCT 1 -- int10--------
    About this table

    HapMap Linkage Disequilibrium images for RAB3A (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 179490

    2 Novoseek disease relationships for RAB3A gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    alzheimers disease 13.76 1 11351135 (1)
    tumors 0.00 4 11074007 (1), 1384950 (1), 19212825 (1)
    About this table

    Human Genome Epidemiology Navigator: RAB3A (1 document)
    Tumor Gene Database: RAB3A

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/90 PubMed articles for RAB3A gene (see all 90 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5864 HGNC: 9777 AceView: RAB3A Ensembl:ENSG00000105649 euGenes: HUgn5864
    ECgene: RAB3A H-InvDB: RAB3A
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB3A Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB3A:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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