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RAB35 Gene

protein-coding   GIFtS: 57

GC12M118995
RAB35, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
H-ray 1, 2
RAB1C 2, 3
RAY 2, 3
Rab-1C 3
Descriptions
GTP-binding protein RAY 3
RAB35, member RAS oncogene family 2
ras-related protein rab-1c (GTP-binding protein ray) 2
External Ids
HGNC: 97741
Entrez Gene: 110212
UniProtKB: Q152863
Ensembl: ENSG000001117377
Search outside databases for aliases for RAB35 gene

Previous GC identifers: GC12M119405 GC12M120060 GC12M120315 GC12M118944

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RAB35_HUMAN, Q15286
Function: Possesses GTPase activity

Gene Wiki entry for RAB35

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB35 gene  

Entrez Gene cytogenetic band: 12q24.31   Ensembl cytogenetic band:  12q24.23   HGNC cytogenetic band: 12q24

RAB35 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M118995:     (about GC identifiers)

Start:
119,017,286 bp from pter
End:
119,038,982 bp from pter
Size:
21,697 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000012.10  NT_009775.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB35_HUMAN, Q15286 (See protein sequence)
Recommended Name: Ras-related protein Rab-35  
Size: 201 amino acids; 23025 Da
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Melanosome.
Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006852.1  

    ENSEMBL proteins: 
    ENSP00000229340 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for RAB35  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (RAB35)
                  OriGene Purified Recombinant Human Protein: RAB35 

    5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IDA16950109
    GO:0005905 coated pit IDA16950109
    GO:0031253 cell projection membrane IDA16950109
    GO:0042470 melanosome IEA--
    GO:0045171 intercellular bridge IDA16950109
    About this table

    Antibodies for RAB35: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for RAB35

    Assays for RAB35: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB35 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q15286

    ProtoNet protein and cluster: Q15286

    1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

    UniProtKB/Swiss-Prot: RAB35_HUMAN, Q15286
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB35)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB35)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006861

                  Applied Biosystems Silencer® siRNAs for RAB35

                  Sigma-Aldrich siRNA for RAB35  
                         Sigma-Aldrich shRNA for RAB35  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006861
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006861
                                     untagged cDNA clone in CMV expression vector: NM_006861 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006861

    UniProtKB/Swiss-Prot: RAB35_HUMAN, Q15286
    Function: Possesses GTPase activity

    Genatlas biochemistry entry for RAB35:
    RAB35,RAS oncogene superfamily member 35,ubiquitously expressed

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003924 GTPase activity TAS7811277
    GO:0005525 GTP binding IEA--
    GO:0005546 phosphatidylinositol-4,5-bisphosphate binding IDA16950109
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/10 Interacting proteins for RAB35 (Q152862) via UniProtKB, MINT, and/or STRING (see all 10 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ACTBP607092MINT-7012070
    C2orf44Q9H6R72MINT-64233
    CBR3O758282MINT-64229
    CDC42P609532MINT-7012095
    CDC42P2P609532MINT-7012095
    About this table

    5 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000910 cytokinesis IMP16950109
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0008104 protein localization IMP16950109
    GO:0015031 protein transport IEA--
    GO:0016197 endosome transport IMP16950109
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB35
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB35

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB35)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB35)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006861

                  Sigma-Aldrich siRNA for RAB35  
                         Sigma-Aldrich shRNA for RAB35  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006861  

    REFSEQ mRNAs for RAB35 gene: 

    NM_006861.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006861  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006861
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006861
                                     untagged cDNA clone in CMV expression vector: NM_006861 

    Additional cDNA sequence: 

    AF498960.1 AK001309.2 AK125543.1 AK298391.1 AK300127.1 AK304620.1 AK312538.1 BC015931.2 
    BC017753.1 BT020024.1 CR536486.1 CR541683.1 X79781.1 

    12 DOTS entries:

    DT.309459  DT.91960376  DT.75159901  DT.100027022  DT.121137122  DT.121137165  DT.91751452  DT.97774884 
    DT.100711889  DT.100757062  DT.100782112  DT.95278290 

    11 AceView cDNA sequences:

    NM_006861 AF498960 AK001309 BC017753 CR622981 CR536486 CR541683 CR623183 
    X79781 BC015931 AK125543 

    highest scoring ESTs for RAB35:

    X79781 AF498960 AK125543 BC015931 BE939625 AA423837 AA423875 AA877614 AK001309 AL534849 

    Unigene Cluster for RAB35:

    RAB35, member RAS oncogene family
    Hs.524788  [show with all ESTs]
    Unigene Representative Sequence: NM_006861


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for RAB35

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c
    SP1:                          -           -                           
    SP2:        -                 -           -                           
    SP3:        -                 -           -     -                     
    SP4:                                      -                           
    SP5:        -                 -                                       

    About this scheme

    ECgene alternative splicing isoforms for RAB35

    1 Ensembl transcript including schematic representation:
    ENST00000229340  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB35 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB35

    1 / 2 / 3

    10 probe-sets matching RAB35 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    47561_at2, 3 U95-B 1 1.00 1.00 0.94 1.04 AA877614 0.60 1.00 0.82 1

    35508_at2, 3 U95-A 1 1.00 1.00 0.34 0.61 X79781 1.00 1.00 1.00 1
    47922_at2, 3 U95-B 1 1.00 1.00 0.77 1.06 AA423837 0.60 1.00 0.82 1

    81598_at2, 3 U95-E 2 1.00 0.97 0.66 1.30 AA423875 0.60 1.00 0.82 1

    205461_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006861 0.60 1.00 0.82 1

    221819_at2, 3 U133-A 1 1.00 1.00 -- -- BF791960 0.40 1.00 0.76 1

    225620_at2, 3 U133-B 2 1.00 0.95 -- -- AL534848 0.20 1.00 0.72 1

    205461_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    221819_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    225620_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
    GeneDecks  RAB35 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AATGGGGGTT

    SOURCE GeneReport for Unigene cluster: Hs.524788

    Expression variation in blood from EXPOLDB for RAB35

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB35 gene from 5/12 species (see all 12 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    cow
    (Bos taurus)
    RAB351   -- RAB35, member RAS oncogene family 91.31(n)
    98.4(a)
    614521  XM_866060.2  XP_871153.1 
    rat
    (Rattus norvegicus)
    Rab351   -- RAB35, member RAS oncogene family 93.86(n)
    100(a)
    288700  NM_001013046.1  NP_001013064.1 
    mouse
    (Mus musculus)
    Rab351, 5 55
    RAB35, member RAS oncogene family1, 5 93.86(n)1
    100(a)1
    774071  NM_198163.11  NP_937806.11 
     AA5713395  AB2326315  (see all 15)
    chicken
    (Gallus gallus)
    RAB351   -- RAB35, member RAS oncogene family 84.74(n)
    98.51(a)
    416983  NM_001030669.1  NP_001025840.1 
    zebrafish
    (Danio rerio)
    BQ450111.12   --   -- 83.12(n)  BQ450111.1 
    About this table        Species with no ortholog for RAB35

    ENSEMBL Gene Tree for RAB35
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB35 gene
    RAB252  RAB11A2  RAB11B2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/129 NCBI SNPs in RAB35 are shown (see all 129 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 42)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs23018831,2
    F,H119040860(+) GGTGTC/TGGGAA 1 -- ng313Minor allele frequency- T:0.01EU EA 298
    --
    rs79722401,2
    C119039185(+) GCCGCC/TACCCT 1 -- ng310--------
    --
    rs175254531,2
    F119038903(-) GCCCGA/GAGGGA 1 -- ut51 ese32Minor allele frequency- G:0.05MN EA 1252
    --
    rs79615471,2
    C119039107(+) CCGGGC/TCGCGC 1 -- ng310--------
    rs71391511,2
    A,C,F,H119030519(+) AGGCAG/AAGGCA 1 -- int14Minor allele frequency- A:0.15EU EA WA 412
    rs79712491,2
    A,C,F,H119021425(+) GGACAG/CTGACC 1 -- int17Minor allele frequency- C:0.50NA EU EA WA 554
    rs64902851,2
    A,C,F,H119032376(+) TCACTC/ACTGTT 1 -- int14Minor allele frequency- A:0.01EU EA WA 418
    rs38525871,2
    A,C,F,H119024673(+) ACACCC/AGGGCG 1 -- int14Minor allele frequency- A:0.06EU EA WA 416
    rs72949751,2
    A,C,F,H119030528(+) CAACTC/GTGTTT 1 -- int14Minor allele frequency- G:0.20EU EA WA 412
    rs22392011,2
    A,C,F,O119023335(+) CGACGT/CTTCCC 1 -- int14Minor allele frequency- C:0.41EA NA 1628
    About this table

    HapMap Linkage Disequilibrium images for RAB35 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604199

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10 PubMed articles for RAB35 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 11021 HGNC: 9774 AceView: RAB35 Ensembl:ENSG00000111737 euGenes: HUgn11021
    ECgene: RAB35 H-InvDB: RAB35
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB35 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB35:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for RAB35-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for RAB35 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Proteins for RAB35
     Lentivirus-delivered shRNAs for RAB35 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Drugs for RAB35
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for RAB35

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