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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

RAB32 Gene

protein-coding   GIFtS: 53
GCID: GC06P146906

RAB32, member RAS oncogene family

 Explore 5 diseases affiliated with
RAB32 via our new
 Human Malady Compendium 
Biological research products
for RAB32
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
RAB32, Member RAS Oncogene Family1 2
Ras-Related Protein Rab-322

External Ids:    HGNC: 97721   Entrez Gene: 109812   Ensembl: ENSG000001185087   OMIM: 6129065   UniProtKB: Q136373   

Export aliases for RAB32 gene to outside databases

Previous GC identifers: GC06P146487 GC06P146711 GC06P146845 GC06P144428


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RAB32:
Small GTP-binding proteins of the RAB family, such as RAB32, play essential roles in vesicle and granule targeting (Bao
et al., 2002 (PubMed 11784320)).(supplied by OMIM, Aug 2009)

UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
Function: Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and
anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Plays a role in the
maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000006.11  NC_018917.1  NT_025741.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RAB32 gene promoter:
         C/EBPbeta   AML1a   Pax-2   Pax-2a   LCR-F1   Evi-1   C/EBPalpha   GATA-1   PPAR-gamma1   PPAR-gamma2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidRAB32 promoter sequence
   Search SABiosciences Chromatin IP Primers for RAB32

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat RAB32


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6q24.3   Ensembl cytogenetic band:  6q24.3   HGNC cytogenetic band: 6q24.2

RAB32 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RAB32 gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P146906:  view genomic region     (about GC identifiers)

Start:
146,864,828 bp from pter      End:
146,876,101 bp from pter
Size:
11,274 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637 (See protein sequence)
Recommended Name: Ras-related protein Rab-32  
Size: 225 amino acids; 24997 Da
Subcellular location: Mitochondrion. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane;
Lipid-anchor; Cytoplasmic side (By similarity). Note=Recruited to phagosomes containing S.aureus or M.tuberculosis

Explore the universe of human proteins at neXtProt for RAB32: NX_Q13637

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q13637

  • RAB32 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_006825.1  
    ENSEMBL proteins: 
     ENSP00000356465  

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    ProSpec Recombinant Protein for RAB32
    Uscn Proteins for RAB32

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005739mitochondrion IEA--
    GO:0030670phagocytic vesicle membrane IEA--
    GO:0045335phagocytic vesicle IDA--


    RAB32 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for RAB32


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RAB32 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR005225 Small_GTP-bd_dom
     IPR001806 Small_GTPase
     IPR003579 Small_GTPase_Rab_type

    Graphical View of Domain Structure for InterPro Entry Q13637

    ProtoNet protein and cluster: Q13637

    1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

    UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
    Similarity: Belongs to the small GTPase superfamily. Rab family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
    Function: Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and
    anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Plays a role in the
    maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis

    miRNA
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    SwitchGear 3'UTR luciferase reporter plasmidRAB32 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for RAB32 (see all 7)
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAB32

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005525GTP binding IEA--


    RAB32 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Sertoli-Sertoli Cell Junction Dynamics
    Epithelial Tight Junctions0.36

    1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for RAB32
        Epithelial Tight Junctions


    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RAB32

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    2 Interacting proteins for RAB32 (Q136373 ENSP000003564654) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PRKAR2AP138613, ENSP000002655634I2D: score=2 STRING: ENSP00000265563
    CHMP243863, ENSP000003503864I2D: score=1 STRING: ENSP00000350386
    About this table

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264small GTPase mediated signal transduction IEA--
    GO:0015031protein transport IEA--
    GO:0090382phagosome maturation IMP--


    RAB32 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RAB32
    Search CenterWatch for drugs/clinical trials and news about RAB32 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for RAB32 gene: 
    NM_006834.3  

    Unigene Cluster for RAB32:

    RAB32, member RAS oncogene family
    Hs.287714  [show with all ESTs]
    Unigene Representative Sequence: BM546299
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000367495(uc003qln.1)

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    hsa-miR-30c hsa-miR-196a* hsa-miR-30d hsa-miR-890 hsa-miR-30a hsa-miR-544b hsa-miR-30b hsa-miR-556-3p
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    Inhib. RNA
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat RAB32
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    Additional cDNA sequence: 

    AF498958.1 AK312202.1 BC015061.1 BT020016.1 U59878.1 U71127.1 

    5 DOTS entries:

    DT.446951  DT.99938168  DT.100751521  DT.40275143  DT.95241235 

    24/149 AceView cDNA sequences (see all 149):

    BQ018663 BM781916 W79890 AI281920 AI354802 AI468087 AI802771 BQ186331 
    AF498958 AW008533 CA424795 BM798652 BM701831 BG166814 AA075275 H25423 
    W74275 AI568482 BF061122 BF940140 BG108028 AA022893 AA746184 BU944029 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    RAB32 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGGCACTTCA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    RAB32 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    LimbZeugopodMesenchymal Condensate CellsBone, Cartilage
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Definitive endoderm-like cells (A scalable, suspensi...)

    See RAB32 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for RAB32

    SOURCE GeneReport for Unigene cluster: Hs.287714

    UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
    Tissue specificity: Widely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal
    lung

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for RAB32

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for RAB32 gene from 6/22 species (see all 22)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves RAB321 RAB32, member RAS oncogene family 81.35(n)
    82.57(a)
      421616  XM_419654.3  XP_419654.1 
    lizard
    (Anolis carolinensis)
    Reptilia RAB326
    --
    80(a)
    1 ↔ 1
    1(207491519-207524943)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.253852 Xenopus laevis transcribed sequence with moderate similarity to protein spQ13637 (H.sapiens) RB32_HUMAN Ras-related protein Rab-32 less 80.66(n)    BJ068994.1 
    zebrafish
    (Danio rerio)
    Actinopterygii rab322 RAB32, member RAS oncogene family 76.15(n)   378969  BC049531.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Rab-RP13
    ltd1
    GTP binding3
    lightoid1
    67(a)3
    65.37(n)1
    69.27(a)1
      359401  NM_165663.21  NP_724768.21 
    worm
    (Caenorhabditis elegans)
    Secernentea glo-11 Protein GLO-1 55.73(n)
    52.82(a)
      3565633  NM_001029666.1  NP_001024837.1 


    ENSEMBL Gene Tree for RAB32 (if available)
    TreeFam Gene Tree for RAB32 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for RAB32 gene
    RAB9A2  RAB7A2  RAB9B2  RAB7L12  RAB382  
    18/56 SIMAP similar genes for RAB32 using alignment to 1 protein entry:     RAB32_HUMAN(see all similar genes):
    RAB38    RAB7L1    RAB3A    RAB7B    RAB11A    RAB39B
    RAB3C    RAB8B    RAB15    RAB19    RAB7A    RAB13
    RAB8A    RAB11B    RAB18    RAB3B    RAB43    RANP1

    RAB32 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/248 NCBI SNPs in RAB32 are shown (see all 248    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1818974161,2
    --146862850(+) ATAAGA/GTTTTT 1 -- us2k10--------
    rs1379152011,2
    C,--146862883(+) GTGTG-/TATATAT 1 -- us2k10--------
    rs353306611,2
    C--146862884(+) TATAT-/ATCTATA 1 -- us2k11Minor allele frequency- AT:0.00NA 2
    rs730127241,2
    C,--146862884(+) TGTGTG/ATATAT 1 -- us2k11Minor allele frequency- A:0.50WA 2
    rs1158329601,2
    --146862889(+) ATATAC/TATATA 1 -- us2k10--------
    rs2021992301,2
    --146862897(+) ATATA-/TCTATAT 1 -- us2k10--------
    rs756038611,2
    F,--146862915(+) TATACG/AGCATG 1 -- us2k11Minor allele frequency- A:0.08WA 118
    rs744060791,2
    F,--146863150(+) TCAGTC/TTTCTG 1 -- us2k11Minor allele frequency- T:0.02NA 120
    rs1435148861,2
    --146863221(+) CAGCAC/TTTACA 1 -- us2k10--------
    rs1378940001,2
    --146863235(+) TACTCC/TCTTAG 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for RAB32 (146864828 - 146876101 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for RAB32: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing RAB32
    DNA2.0 Custom Variant and Variant Library Synthesis for RAB32

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    RAB32 for disorders           About GeneDecksing

    OMIM gene information: 612906    OMIM disorders: --

    5 diseases for RAB32:    About MalaCards
    hermansky-pudlak syndrome    inflammatory bowel disease    endometrial adenocarcinoma    leprosy
    adenocarcinoma

    1 disease from the University of Copenhagen DISEASES database for RAB32:
    Hermansky-Pudlak syndrome
    Human Genome Epidemiology (HuGE) Navigator: RAB32 (1 document)

    Export disorders for RAB32 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for RAB32 gene, integrated from 9 sources (see all 19):
    (articles sorted by number of sources associating them with RAB32)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    2. The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)1, 2 Mungall A.J.... Beck S. (2003)
    3. Molecular cloning, bacterial expression and properties of Rab31 and Rab32. (PubMed id 11784320)1, 2 Bao X.... Haslam R.J. (2002)
    4. Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics. (PubMed id 12186851)1, 2 Alto N.M.... Scott J.D. (2002)
    5. BLOC-2, AP-3, and AP-1 proteins function in concert wi th Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related o rganelles. (PubMed id 22511774)1 Bultema J.J....Di Pietro S.M. (2012)
    6. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    7. Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. (PubMed id 21255211)2 Seto S.... Koide Y. (2011)
    8. Identification of two new loci at IL23R and RAB32 tha t influence susceptibility to leprosy. (PubMed id 22019778)1 Zhang F....Zhang X. (2011)
    9. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    10. Genetic variants in nuclear-encoded mitochondrial gen es influence AIDS progression. (PubMed id 20877624)1 Hendrickson S.L....O'Brien S.J. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 10981 HGNC: 9772 AceView: RAB32 Ensembl:ENSG00000118508 euGenes: HUgn10981
    ECgene: RAB32 H-InvDB: RAB32

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for RAB32 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for RAB32 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for RAB32 gene:
    Search GeneIP for patents involving RAB32

    GeneCards and IP:
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