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RAB32 Gene

protein-coding   GIFtS: 57

GC06P146906
RAB32, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
OTTHUMP00000017369 2
Descriptions
RAB32, member RAS oncogene family 2
External Ids
HGNC: 97721
Entrez Gene: 109812
UniProtKB: Q136373
Ensembl: ENSG000001185087
Search outside databases for aliases for RAB32 gene

Previous GC identifers: GC06P146487 GC06P146711 GC06P146845

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
Function: Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of
protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of
mitochondrial fission

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB32 gene  

Entrez Gene cytogenetic band: 6q24.3   Ensembl cytogenetic band:  6q24.3   HGNC cytogenetic band: 6q24.2

RAB32 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P146906:     (about GC identifiers)

Start:
146,906,521 bp from pter
End:
146,917,782 bp from pter
Size:
11,262 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000006.10  NT_025741.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637 (See protein sequence)
Recommended Name: Ras-related protein Rab-32  
Size: 225 amino acids; 24997 Da
Subcellular location: Mitochondrion

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006825.1  

    ENSEMBL proteins: 
    ENSP00000356465 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (RAB32)
    Human Recombinant Proteins from Abnova (RAB32)
                  OriGene Purified Recombinant Human Protein: RAB32 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005739 mitochondrion IEA--
    About this table

    Antibodies for RAB32: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for RAB32
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (RAB32), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (RAB32)
    Novus Biologicals Antibodies for RAB32

    Assays for RAB32: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB32 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q13637

    ProtoNet protein and cluster: Q13637

    1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

    UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB32)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB32)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006834

                  Applied Biosystems Silencer® siRNAs for RAB32

                  Sigma-Aldrich siRNA and siRNA Panels for RAB32  
                         Sigma-Aldrich shRNA for RAB32  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for RAB32
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006834
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006834
                                     untagged cDNA clone in CMV expression vector: NM_006834 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006834

    UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
    Function: Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of
    protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of
    mitochondrial fission

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for RAB32 (ENSP000003564653) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PRKAR2AENSP000002655633STRING (score=.883)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0015031 protein transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB32
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB32

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB32)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB32)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006834

                  Sigma-Aldrich siRNA and siRNA Panels for RAB32  
                         Sigma-Aldrich shRNA for RAB32  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006834  

    REFSEQ mRNAs for RAB32 gene: 

    NM_006834.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006834  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006834
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006834
                                     untagged cDNA clone in CMV expression vector: NM_006834 

    Additional cDNA sequence: 

    AF498958.1 AK312202.1 BC015061.1 BT020016.1 U59878.1 U71127.1 

    5 DOTS entries:

    DT.446951  DT.99938168  DT.100751521  DT.40275143  DT.95241235 

    24/149 AceView cDNA sequences (see all 149 ):

    W79890 AA075275 AA746184 BG108028 AI468087 BF940140 CA424795 AA022893 
    AI354802 W74275 BG166814 AI568482 BM781916 BU944029 BF061122 BM701831 
    AW008533 AI802771 AI281920 AF498958 H25423 BM798652 BQ018663 BQ186331 

    highest scoring ESTs for RAB32:

    U59878 AA022893 AA022894 AA036761 AA055902 AA055990 AA055997 AA055999 AA075163 AA075275 

    Unigene Cluster for RAB32:

    RAB32, member RAS oncogene family
    Hs.287714  [show with all ESTs]
    Unigene Representative Sequence: BM546299


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000367495  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB32 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB32

    1 / 2 / 3

    3 probe-sets matching RAB32 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    41523_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 U59878 1.00 1.00 1.00 1

    204214_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006834 0.60 1.00 0.82 1

    204214_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGGCACTTCA

    SOURCE GeneReport for Unigene cluster: Hs.287714

    Expression variation in blood from EXPOLDB for RAB32

    UniProtKB/Swiss-Prot: RAB32_HUMAN, Q13637
    Tissue specificity: Widely expressed with high levels in heart, liver, kidney, bone marrow, testis,
    colon and fetal lung

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB32 gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    RAB321   -- RAB32, member RAS oncogene family 99.65(n)
    100(a)
    748474  XM_001161467.1  XP_001161467.1 
    cow
    (Bos taurus)
    RAB321   -- RAB32, member RAS oncogene family 89.58(n)
    89.29(a)
    528099  XM_606510.3  XP_606510.2 
    rat
    (Rattus norvegicus)
    Rab321   -- RAB32, member RAS oncogene family 84.01(n)
    84.3(a)
    365042  XM_344794.3  XP_344795.2 
    mouse
    (Mus musculus)
    Rab321, 5 105
    RAB32, member RAS oncogene family1, 5 85.35(n)1
    84.75(a)1
    678441  NM_026405.31  NP_080681.11 
     AA8811105  AB2326275  (see all 23)
    chicken
    (Gallus gallus)
    RAB321   -- RAB32, member RAS oncogene family 81.35(n)
    82.57(a)
    421616  XM_419654.2  XP_419654.1 
    About this table        Species with no ortholog for RAB32

    ENSEMBL Gene Tree for RAB32
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB32 gene
    RAB7L12  RAB382  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/80 NCBI SNPs in RAB32 are shown (see all 80 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 27)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 6 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs69157311,2
    A,C,H146905098(+) tccgtA/Gtgtgt 1 -- ng511Minor allele frequency- G:0.00WA 120
    --
    rs18533201,2
    C146918092(+) CAAACA/GAACCT 1 -- ng310--------
    --
    rs135741,2
    H146917398(+) CAAGAG/TACCTT 1 E/D mis15Minor allele frequency- T:0.00MN EU EA WA 590
    rs38042881,2
    A,C,F,H146909741(-) GCTACA/GTGTGT 1 -- int15Minor allele frequency- G:0.44EA EU WA 1148
    --
    rs38042891,2
    A,C,F,H146909654(-) TACCTT/CGCCCT 1 -- int14Minor allele frequency- C:0.43EU EA WA 404
    rs93770041,2
    A,C,F,H146915307(+) AGGCTA/GCAATG 1 -- int14Minor allele frequency- G:0.40EU EA WA 418
    rs111554751,2
    A,C,F,H146914378(+) TGCCTA/GTTGGG 1 -- int114Minor allele frequency- G:0.33EU EA WA NA 1440
    rs38042821,2
    C,F,H146917015(-) CTCGAT/CGGTTG 1 -- int118Minor allele frequency- C:0.29EA NS EU WA NA 2618
    rs38042851,2
    C,F,H146913726(-) cacaAG/TAAATC 1 -- int15Minor allele frequency- T:0.03EA EU WA 1900
    rs38042841,2
    C,F,H146913895(-) GCTTCG/ACTTTT 1 -- int19Minor allele frequency- A:0.10EA NA EU WA 2102
    About this table

    HapMap Linkage Disequilibrium images for RAB32 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for RAB32 gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10981 HGNC: 9772 AceView: RAB32 Ensembl:ENSG00000118508 euGenes: HUgn10981
    ECgene: RAB32 H-InvDB: RAB32
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB32 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB32:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for RAB32 Antibodies for RAB32
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for RAB32 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Custom Service for Rabbit Pab from Full-length Protein
      
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     Recombinant Proteins
    (RAB32)
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     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for RAB32




     Drugs for RAB32
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for RAB32

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