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RAB2B Gene

protein-coding   GIFtS: 53

GC14M020997
RAB2B, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ14824 1, 2
OTTHUMP00000195135 2
Descriptions
GTP-binding protein RAB2B 2
RAB2B protein 2
RAB2B, member RAS oncogene family 2
RAS family, member RAB2B 2
External Ids
HGNC: 202461
Entrez Gene: 849322
UniProtKB: Q8WUD13
Ensembl: ENSG000001294727
Search outside databases for aliases for RAB2B gene

Previous GC identifers: GC14M015714 GC14M019917

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for RAB2B:
Members of the Rab protein family are nontransforming monomeric GTP-binding proteins of the Ras
superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. Rab
proteins are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking; see
MIM 179508.[supplied by OMIM]

UniProtKB/Swiss-Prot: RAB2B_HUMAN, Q8WUD1
Function: Required for protein transport from the endoplasmic reticulum to the Golgi complex
(Potential)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB2B gene  

Entrez Gene cytogenetic band: 14q11.2   Ensembl cytogenetic band:  14q11.2   HGNC cytogenetic band: 14q11.1

RAB2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M020997:     (about GC identifiers)

Start:
20,997,019 bp from pter
End:
21,014,972 bp from pter
Size:
17,954 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB2B_HUMAN, Q8WUD1 (See protein sequence)
Recommended Name: Ras-related protein Rab-2B  
Size: 216 amino acids; 24214 Da
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Endoplasmic
reticulum membrane (Potential). Golgi apparatus membrane (Potential)
PDB structures from and Proteopedia :
2A5J (3D)    

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_116235.2  

    ENSEMBL proteins: 
    ENSP00000380869 ENSP00000302005 ENSP00000250404 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for RAB2B  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (RAB2B)
    Human Recombinant Proteins from Abnova (RAB2B)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane IEA--
    GO:0005783 endoplasmic reticulum IEA--
    GO:0005789 endoplasmic reticulum membrane IEA--
    GO:0005794 Golgi apparatus IEA--
    GO:0005886 plasma membrane IEA--
    About this table

    Antibodies for RAB2B: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for RAB2B
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (RAB2B), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (RAB2B)
    Novus Biologicals Antibodies for RAB2B

    Assays for RAB2B: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB2B for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8WUD1

    ProtoNet protein and cluster: Q8WUD1

    UniProtKB/Swiss-Prot: RAB2B_HUMAN, Q8WUD1
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB2B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB2B)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032846

                  Applied Biosystems Silencer® siRNAs for RAB2B

                  Sigma-Aldrich siRNA for RAB2B  
                         Sigma-Aldrich shRNA for RAB2B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for RAB2B
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_032846
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_032846
                                     untagged cDNA clone in CMV expression vector: NM_032846 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_032846

    UniProtKB/Swiss-Prot: RAB2B_HUMAN, Q8WUD1
    Function: Required for protein transport from the endoplasmic reticulum to the Golgi complex
    (Potential)

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0015031 protein transport IEA--
    GO:0016192 vesicle-mediated transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB2B
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB2B

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB2B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB2B)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032846

                  Sigma-Aldrich siRNA for RAB2B  
                         Sigma-Aldrich shRNA for RAB2B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_032846  

    REFSEQ mRNAs for RAB2B gene: 

    NM_032846.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_032846  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_032846
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_032846
                                     untagged cDNA clone in CMV expression vector: NM_032846 

    Additional cDNA sequence: 

    AF091029.1 AF468652.1 AK027730.1 AK296057.1 AK300599.1 AK307349.1 AK315354.1 BC020839.1 
    BC066366.1 BX647601.1 

    13 DOTS entries:

    DT.120763180  DT.415691  DT.99980592  DT.91732287  DT.100027172  DT.100027170  DT.100759573  DT.120763211 
    DT.100641376  DT.120763131  DT.40132312  DT.100759576  DT.120763158 

    24/134 AceView cDNA sequences (see all 134 ):

    BM556073 CD634472 AA738182 BG034030 AA885791 BM263225 AA961980 AF091029 
    AI831064 BG621213 BU679539 CB068930 AI217828 BG291037 BE935943 AW236302 
    AW191905 AK027730 AF468652 BG502390 BX109061 AW517002 BI596579 BQ002987 

    highest scoring ESTs for RAB2B:

    AA088536 AA531016 AF468652 BC020839 BF977443 BF979487 BG699157 BM271844 BM556073 BU658077 

    Unigene Cluster for RAB2B:

    RAB2B, member RAS oncogene family
    Hs.22399  [show with all ESTs]
    Unigene Representative Sequence: BC066366


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for RAB2B

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8a · 8b
    SP1:                    -           -           -                                                   
    SP2:                    -           -           -     -                                             
    SP3:                    -           -                                                               
    SP4:                                -           -                                                   
    SP5:                    -                                                                           

    About this scheme

    ECgene alternative splicing isoforms for RAB2B

    3 Ensembl transcripts including schematic representations:
    ENST00000397762  ENST00000304034  ENST00000250404  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB2B expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB2B

    1 / 2 / 3

    5 probe-sets matching RAB2B gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    78609_at2, 3 U95-E 1 1.00 1.00 0.93 1.09 AA088536 0.80 1.00 0.91 1

    84076_at2, 3 U95-E 1 1.00 1.00 0.94 0.66 W28130 0.60 1.00 0.82 1

    49516_at2, 3 U95-B 1 0.94 1.00 0.86 1.26 AA531016 0.80 1.00 0.91 1

    225074_at2, 3 U133-B 1 1.00 1.00 -- -- AA531016 0.80 1.00 0.91 1

    225074_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  RAB2B for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TATCAAAGCT

    SOURCE GeneReport for Unigene cluster: Hs.22399

    UniProtKB/Swiss-Prot: RAB2B_HUMAN, Q8WUD1
    Tissue specificity: Expressed in kidney, prostate, lung, liver, thymus, colon, pancreas, and
    skeletal muscle, and low levels in placenta. Not detected in heart, brain, spleen, testis, ovary,
    small intestine and leukocyte

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB2B gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RAB2B1   -- RAB2B, member RAS oncogene family 93.67(n)
    97.69(a)
    475402  XM_532625.2  XP_532625.2 
    cow
    (Bos taurus)
    RAB2B1   -- RAB2B, member RAS oncogene family 94.44(n)
    96.76(a)
    528574  XM_607000.3  XP_607000.2 
    rat
    (Rattus norvegicus)
    Rab2b1   -- RAB2B, member RAS oncogene family 91.47(n)
    95.81(a)
    305853  NM_001037645.1  NP_001032734.1 
    mouse
    (Mus musculus)
    Rab2b1, 5 145
    RAB2B, member RAS oncogene family1, 5 91.2(n)1
    94.91(a)1
    763381  NM_172601.21  NP_766189.11 
     AA4723125  AB2325865  (see all 23)
    African clawed frog
    (Xenopus laevis)
    Xl.27492   -- Xenopus laevis transcribed sequence with moderate similarity more 78.6(n)  CA790266.1 
    About this table        Species with no ortholog for RAB2B

    ENSEMBL Gene Tree for RAB2B
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB2B gene
    RAB2A2  RAB142  RAB4A2  RAB4B2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/107 NCBI SNPs in RAB2B are shown (see all 107 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 14 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10458421,2
    A,C,F,H,O20997116(-) GTCCCA/GTTCTC 1 -- ut31 ese38Minor allele frequency- G:0.07MN EU EA WA NA 746
    rs37900701,2
    A,C,F,H20996687(+) AACAAA/CAAAAA 1 -- ng512Minor allele frequency- C:0.05EA EU 1412
    rs49813721,2
    A,C,F,H20997478(+) ATGAGT/CAGCAC 1 -- ut31 ese311Minor allele frequency- C:0.22NA EU EA WA 966
    rs10458111,2
    C,F,H20998223(-) TTGCAA/GTTACC 1 -- ut3111Minor allele frequency- G:0.23NA EU EA WA 982
    rs171064111,2
    C,F,H20999211(+) CAGAGT/GTGGAC 1 T/N mis1 ese311Minor allele frequency- G:0.02NA EU EA WA 982
    rs10458121,2
    C,F,H20998178(-) GCCTCG/AGAATT 1 -- ut3111Minor allele frequency- A:0.21NA EU EA WA 976
    rs80135001,2
    A,C,F,H21001039(+) ATCAAT/CACCAG 1 -- int18Minor allele frequency- C:0.30EU EA WA 838
    rs80051631,2
    A,C,F,H21012591(+) TTTATA/GTATCA 1 -- int18Minor allele frequency- G:0.19EU EA WA 832
    rs65718461,2
    A,C,F,H21010726(+) CTTTCT/CTTTTT 1 -- int1 trp38Minor allele frequency- C:0.21EU EA WA 838
    rs101461181,2
    A,C,F,H21008624(+) TTACAC/GTTTTA 1 -- int14Minor allele frequency- G:0.29EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for RAB2B (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607466

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    5 PubMed articles for RAB2B gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 84932 HGNC: 20246 AceView: RAB2B Ensembl:ENSG00000129472 euGenes: HUgn84932
    ECgene: RAB2B H-InvDB: RAB2B
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB2B Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB2B:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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