V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

RAB28 Gene

protein-coding   GIFtS: 54

GC04M013045
RAB28, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
MGC41862 2
Rab-26 3
Descriptions
RAB28, member RAS oncogene family 2
Ras-related protein Rab-28 2
External Ids
HGNC: 97681
Entrez Gene: 93642
UniProtKB: P511573
Ensembl: ENSG000001578697
Search outside databases for aliases for RAB28 gene

Previous GC identifers: GC04M013212 GC04U900009 GC04M013057 GC04M013120

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for RAB28:
This gene encodes a member of the Rab subfamily of Ras-related small GTPases. The encoded protein
may be involved in regulating intracellular trafficking. Alternative splicing results in multiple
transcript variants. Pseudogenes of this gene are found on chromosomes 9 and X. [provided by
RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB28 gene  

Entrez Gene cytogenetic band: 4p15.33   Ensembl cytogenetic band:  4p15.33   HGNC cytogenetic band: 4p16.1

RAB28 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04M013045:     (about GC identifiers)

Start:
12,978,447 bp from pter
End:
13,095,060 bp from pter
Size:
116,614 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000004.10  NT_006316.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: RAB28_HUMAN, P51157 (See protein sequence)
Recommended Name: Ras-related protein Rab-28  
Size: 221 amino acids; 24841 Da
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential)
PDB structure from and Proteopedia :
2HXS (3D)    3E5H (3D)    
Secondary accessions: Q8IYR8 Q8NI05
Alternative splicing: 2 isoforms:  P51157-1   P51157-2   

REFSEQ proteins (3 alternative transcripts): 
NP_001017979.1  NP_001153073.1  NP_004240.2  


ENSEMBL proteins: 
ENSP00000288723 ENSP00000340079 ENSP00000349060 ENSP00000328551 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (RAB28)
Human Recombinant Proteins from Abnova (RAB28)
              OriGene Purified Recombinant Human Protein: RAB28 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane IEA--
About this table

Antibodies for RAB28: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (RAB28), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (RAB28)
Novus Biologicals Antibodies for RAB28

Assays for RAB28: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

3 InterPro domains/families:
 IPR001806 Ras_GTPase
 IPR005225 Small_GTP_bd
 IPR013753 Ras


   GeneDecks  RAB28 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry P51157

ProtoNet protein and cluster: P51157

1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

UniProtKB/Swiss-Prot: RAB28_HUMAN, P51157
Similarity: Belongs to the small GTPase superfamily. Rab family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB28)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB28)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_001017979

              Applied Biosystems Silencer® siRNAs for RAB28

              Sigma-Aldrich siRNA for RAB28  
                     Sigma-Aldrich shRNA for RAB28  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for RAB28
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001017979
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001017979
                                 untagged cDNA clones in CMV expression vector (see all 3): BC018067 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001017979

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003924 GTPase activity TAS8647132
GO:0005525 GTP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007264 small GTPase mediated signal transduction IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for RAB28
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for RAB28

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB28)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB28)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_001017979

              Sigma-Aldrich siRNA for RAB28  
                     Sigma-Aldrich shRNA for RAB28  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001017979  NM_004249  

REFSEQ mRNAs for RAB28 gene (3 alternative transcripts): 

NM_001017979.2   NM_001159601.1   NM_004249.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001017979  NM_004249  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001017979
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001017979
                                 untagged cDNA clones in CMV expression vector (see all 3): BC018067 

Additional cDNA sequence: 

AF498955.1 BC018067.1 BC035054.2 BX537681.1 CR592237.1 CR597076.1 CR612152.1 CR616293.1 
CR621879.1 X94703.1 

12 DOTS entries:

DT.95074907  DT.217999  DT.91923892  DT.217998  DT.95309902  DT.100008711  DT.121251983  DT.91747764 
DT.95181071  DT.121252012  DT.40274367  DT.95309905 

24/111 AceView cDNA sequences (see all 111 ):

CR612152 BQ021120 CR592237 BU742941 AI215961 Z41536 N46403 AI015919 
AK027027 BM801937 AI689194 CR621879 N50159 AI084521 CR616293 CF456155 
BU630749 AA666268 BM920172 Z45911 N49476 BU172445 W72006 BM832340 

highest scoring ESTs for RAB28:

X94703 AF498955 BC018067 BG706085 CB565910 N37088 R52316 AA134142 AA666268 AI084521 

Unigene Cluster for RAB28:

RAB28, member RAS oncogene family
Hs.656060  [show with all ESTs]
Unigene Representative Sequence: BX537681


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for RAB28

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b
SP1:                                            -     -               
SP2:                    -     -                 -     -               
SP3:                                            -                     
SP4:                                                                  

About this scheme

ECgene alternative splicing isoforms for RAB28

4 Ensembl transcripts including schematic representations:
ENST00000288723  ENST00000338176  ENST00000356643  ENST00000330852  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

RAB28 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RAB28

1 / 2 / 3

8 probe-sets matching RAB28 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

44794_s_at2, 3 U95-B 1 1.00 1.00 0.84 1.47 N37088 0.80 1.00 0.91 1

35053_at2, 3 U95-A 2 1.00 0.97 0.94 0.54 X94703 1.00 1.00 1.00 1

227003_at2, 3 U133-B 1 1.00 1.00 -- -- BG150494 0.60 1.00 0.82 1

209084_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

207495_at2, 3 U133-A 6 1.00 0.87 -- -- NM_004249 0.60 1.00 0.82 1

227003_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209084_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

207495_at2 U133Plus2 6 1.00 0.87 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TCGGACTCTG

SOURCE GeneReport for Unigene cluster: Hs.656060

Expression variation in blood from EXPOLDB for RAB28

UniProtKB/Swiss-Prot: RAB28_HUMAN, P51157
Tissue specificity: Isoform S is detected in most tissues investigated (cortex, liver, kidney,
skeletal muscle, adipose tissue, testis and urothelium), whereas isoform L is predominant in
testis

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RAB28 gene from 5/10 species (see all 10 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RAB281   -- RAB28, member RAS oncogene family 95.48(n)
96.38(a)
477884  XM_535076.2  XP_535076.2 
chimpanzee
(Pan troglodytes)
RAB281   -- RAB28, member RAS oncogene family 98.36(n)
98.03(a)
461120  XM_517110.2  XP_517110.2 
cow
(Bos taurus)
RAB281   -- RAB28, member RAS oncogene family 95.78(n)
96.38(a)
536021  NM_001034634.1  NP_001029806.1 
rat
(Rattus norvegicus)
Rab281   -- RAB28, member RAS oncogene family 92.61(n)
96.38(a)
117049  NM_053978.1  NP_446430.1 
mouse
(Mus musculus)
Rab281, 5 55
RAB28, member RAS oncogene family1, 5 92.01(n)1
95.48(a)1
1009721  NM_027295.21  NP_081571.11 
 AB2326245  AI8420665  (see all 13)
About this table        Species with no ortholog for RAB28

ENSEMBL Gene Tree for RAB28
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Pseudogenes
1 related pseudogene


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/496 NCBI SNPs in RAB28 are shown (see all 496 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 190)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 4 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs100237501,2
A,F,H13097048(+) TTATTA/GGGTAA 1 -- ng314Minor allele frequency- G:0.08EU EA WA 418
rs117335141,2
A,C,F13096799(+) TAGTGA/TGAAGA 2 -- ng312Minor allele frequency- T:0.20NA 182
rs100316231,2
A,F,H12978422(+) GATGTC/TTGAAA 1 -- ng514Minor allele frequency- T:0.03EU EA WA 418
rs117363941,2
A,C13096716(+) ATCCCC/GCACCA 2 -- ng310--------
--
rs117335231,2
A13096834(+) TAACCA/CCTTCT 2 -- ng310--------
--
rs100011141,2
A12978713(+) GGCCCC/TTTTTT 1 -- ut310--------
--
rs68327411,2
C13096379(+) TCTCAG/TGTTTA 2 -- ng310--------
rs21095901,2
A,C,F,H,O12986384(+) AATGAT/CGGGAA 2 -- int17Minor allele frequency- C:0.44EU EA WA NA 560
rs11253191,2
A,C,F,H,O12984014(+) AACAAC/TCTGGG 2 -- int115Minor allele frequency- T:0.23EA NA NS EU WA 712
rs14566861,2
C,F,H,O12981418(+) TCCCCC/AATTCC 2 -- int14Minor allele frequency- A:0.01EU NA 256
About this table

HapMap Linkage Disequilibrium images for RAB28 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

8 PubMed articles for RAB28 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9364 HGNC: 9768 AceView: RAB28 Ensembl:ENSG00000157869 euGenes: HUgn9364
ECgene: RAB28 H-InvDB: RAB28
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for RAB28 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RAB28:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for RAB28-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for RAB28 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for RAB28 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Recombinant Proteins (RAB28)
 Antibodies (RAB28)
 Chimera RNAi (RAB28)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(RAB28)
 Antibodies (RAB28)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for RAB28




 Drugs for RAB28
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for RAB28

GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

Random Gene From GIFtS : about GIFtS

Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science



The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
RAB28 Gene at Home site.
Version 2.41.1
server4.xennexinc.com