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RAB27B Gene

protein-coding   GIFtS: 56

GC18P050646
RAB27B, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
C25KG 3
Descriptions
RAB27B, member RAS oncogene family 2
External Ids
HGNC: 97671
Entrez Gene: 58742
UniProtKB: O001943
Ensembl: ENSG000000413537
Search outside databases for aliases for RAB27B gene

Previous GC identifers: GC18P052584 GC18P052398 GC18P052280 GC18P050644

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RB27B_HUMAN, O00194
Function: May be involved in targeting uroplakins to urothelial apical membranes (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB27B gene  

Entrez Gene cytogenetic band: 18q21.2   Ensembl cytogenetic band:  18q21.2   HGNC cytogenetic band: 18q21.2

RAB27B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 18         GeneLoc Exon Structure

GeneLoc location for GC18P050646:     (about GC identifiers)

Start:
50,646,706 bp from pter
End:
50,708,210 bp from pter
Size:
61,505 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000018.8  NT_025028.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: RB27B_HUMAN, O00194 (See protein sequence)
Recommended Name: Ras-related protein Rab-27B  
Size: 218 amino acids; 24608 Da
Subunit: Binds SYTL4 and MYRIP (By similarity)
Subcellular location: Membrane; Lipid-anchor
PDB structures from and Proteopedia :
2F7S (3D)    
Secondary accessions: Q9BZB6

REFSEQ proteins: NP_004154.2  

ENSEMBL proteins: 
ENSP00000262094 


Human Recombinant Proteins 
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Browse Purified and Recombinant Proteins at Millipore
Sigma-Aldrich AQUA Peptides for RAB27B  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (RAB27B)
Human Recombinant Proteins from Abnova (RAB27B)
              OriGene Purified Recombinant Human Protein: RAB27B 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
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Antibodies for RAB27B: 
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Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for RAB27B
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Antibodies from Abcam (RAB27B), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (RAB27B)
Novus Biologicals Antibodies for RAB27B

Assays for RAB27B: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5 InterPro domains/families:
 IPR015597 Rab27
 IPR001806 Ras_GTPase
 IPR005225 Small_GTP_bd
 IPR003579 GTPase_Rab
 IPR013753 Ras


   GeneDecks  RAB27B for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry O00194

ProtoNet protein and cluster: O00194

1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

UniProtKB/Swiss-Prot: RB27B_HUMAN, O00194
Similarity: Belongs to the small GTPase superfamily. Rab family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB27B)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB27B)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004163

              Applied Biosystems Silencer® siRNAs for RAB27B

              Sigma-Aldrich siRNA for RAB27B  
                     Sigma-Aldrich shRNA for RAB27B  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004163
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004163
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_004163 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_004163

UniProtKB/Swiss-Prot: RB27B_HUMAN, O00194
Function: May be involved in targeting uroplakins to urothelial apical membranes (By similarity)

9 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Rab27b):

endocrine/exocrine glandhematopoietic systemhomeostasis/metabolismnervous systemno phenotypic analysis
normalpigmentationreproductive systemskin/coat/nails

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003924 GTPase activity TAS9066979
GO:0005515 protein binding IEA--
GO:0005525 GTP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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 Gene Network CentralTM Interacting Genes and Proteins Network for  RAB27B 


5/9 Interacting proteins for RAB27B (ENSP000002620943) via UniProtKB, MINT, and/or STRING (see all 9 )
InteractantInteraction Details
GeneCardExternal ID(s)
MLPHENSP000002646053STRING (score=.989)
SYTL1ENSP000003164643STRING (score=.985)
MYO5AENSP000003509453STRING (score=.944)
MYRIPENSP000003019723STRING (score=.906)
SYTL4ENSP000002761413STRING (score=.894)
About this table

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007264 small GTPase mediated signal transduction IEA--
GO:0015031 protein transport IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for RAB27B
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for RAB27B

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB27B)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB27B)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004163

              Sigma-Aldrich siRNA for RAB27B  
                     Sigma-Aldrich shRNA for RAB27B  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_004163  

REFSEQ mRNAs for RAB27B gene: 

NM_004163.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_004163  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004163
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004163
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_004163 

Additional cDNA sequence: 

AF131784.1 AF329499.1 AF498954.1 AK314115.1 BC027474.1 U57093.2 

6 DOTS entries:

DT.40110267  DT.307484  DT.97816796  DT.121084137  DT.91695544  DT.97837249 

24/59 AceView cDNA sequences (see all 59 ):

BC027474 BU618369 AF329499 BF002851 NM_004163 AF498954 CB565911 AL707788 
BX281002 AI277199 BQ723291 CA335387 BF438386 BG195281 AA846320 BG620768 
BM985425 BU752963 BQ020683 AF131784 AW340618 AW468475 AW664866 BX952196 

highest scoring ESTs for RAB27B:

AI432357 U57093 AA812916 AF329499 AF498954 AW592937 BC027474 BG195281 BG284004 BU618369 

Unigene Cluster for RAB27B:

RAB27B, member RAS oncogene family
Hs.25318  [show with all ESTs]
Unigene Representative Sequence: AF131784


GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for RAB27B

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6
SP1:                                    
SP2:                    -               

About this scheme

ECgene alternative splicing isoforms for RAB27B

1 Ensembl transcript including schematic representation:
ENST00000262094  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

RAB27B expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RAB27B

1 / 2 / 3

6 probe-sets matching RAB27B gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

64838_at2, 3 U95-C 1 1.00 1.00 0.78 1.36 AI432357 1.00 1.00 1.00 1

808_at2, 3 U95-A 2 1.00 0.97 0.90 0.80 U57093 1.00 1.00 1.00 1

207017_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004163 0.60 1.00 0.82 1

207018_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004163 0.60 1.00 0.82 1

207017_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

207018_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TACAAATAGG

SOURCE GeneReport for Unigene cluster: Hs.25318

Expression variation in blood from EXPOLDB for RAB27B

UniProtKB/Swiss-Prot: RB27B_HUMAN, O00194
Tissue specificity: Expressed primarily in testis

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RAB27B gene from 5/11 species (see all 11 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RAB27B1   -- RAB27B, member RAS oncogene family 91.59(n)
96.79(a)
483974  XM_541092.1  XP_541092.1 
chimpanzee
(Pan troglodytes)
RAB27B1   -- RAB27B, member RAS oncogene family 99.85(n)
99.54(a)
468542  XM_523931.2  XP_523931.2 
cow
(Bos taurus)
RAB27B1   -- RAB27B, member RAS oncogene family 90.52(n)
93.58(a)
282858  NM_174738.2  NP_777163.1 
rat
(Rattus norvegicus)
Rab27b1   -- RAB27B, member RAS oncogene family 88.07(n)
93.58(a)
84590  NM_053459.1  NP_445911.1 
mouse
(Mus musculus)
Rab27b1, 5 18 (45.00 cM)5
RAB27b, member RAS oncogene family1, 5 88.99(n)1
94.5(a)1
807181  NM_001082553.11  NP_001076022.11 
 AB0677755  AB1205125  (see all 21)
About this table        Species with no ortholog for RAB27B

ENSEMBL Gene Tree for RAB27B
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for RAB27B gene
RAB27A2  RAB262  RAB372  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/408 NCBI SNPs in RAB27B are shown (see all 408 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 134)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 18 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs19443581,2
A,C,F,H50708240(-) CTCCTA/GCATTT 1 -- ng314Minor allele frequency- G:0.03EU EA WA 418
rs48010991,2
A,C,F,H50646135(+) atactT/Ggttca 1 -- ng514Minor allele frequency- G:0.05EU EA WA 418
rs20142061,2
A,C,F,H50645975(+) GTACCC/TAACCT 1 -- ng514Minor allele frequency- T:0.05EU EA WA 418
rs37645121,2
C,F,H50646322(-) GGCCTC/AGGGAA 1 -- ng514Minor allele frequency- A:0.21EU EA WA 418
rs19443561,2
C,F,H50708314(-) ACTTCG/ATCATT 1 -- ng3113Minor allele frequency- A:0.03MN NA EU EA WA 1250
--
rs20142371,2
A,C50645581(+) CACTAC/TGAAGA 1 -- ng510--------
rs37645161,2
C,F50645372(-) ATGTAG/TGCTTG 1 -- ng513Minor allele frequency- T:0.32NA 118
rs99662651,2
C,H50702596(+) GAGACG/ACCATG 1 T/A mis1 ese34Minor allele frequency- A:0.00EU EA WA 420
rs37645151,2
A,C50645395(-) CTCAGA/CCTGTA 1 -- ng510--------
--
rs37645131,2
C50646087(-) acacaC/Ttagat 1 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for RAB27B (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 603869

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/17 PubMed articles for RAB27B gene (see all 17 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 5874 HGNC: 9767 AceView: RAB27B Ensembl:ENSG00000041353 euGenes: HUgn5874
ECgene: RAB27B H-InvDB: RAB27B
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for RAB27B Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RAB27B:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Search Tocris compounds for RAB27B




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