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RAB25 Gene

protein-coding   GIFtS: 51

GC01P154297
RAB25, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CATX-8 1, 2, 3
CATX8 3
OTTHUMP00000018847 2
Descriptions
RAB25, member RAS oncogene family 2
External Ids
HGNC: 182381
Entrez Gene: 571112
UniProtKB: P577353
Ensembl: ENSG000001326987
Search outside databases for aliases for RAB25 gene

Previous GC identifers: GC01P153899 GC01P151796 GC01P152809 GC01P153247 GC01P152844

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: RAB25_HUMAN, P57735
Function: May selectively regulate the apical recycling and/or transcytotic pathways (By
similarity)

Gene Wiki entry for RAB25

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB25 gene  

Entrez Gene cytogenetic band: 1q22   Ensembl cytogenetic band:  1q22   HGNC cytogenetic band: 1q22

RAB25 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P154297:     (about GC identifiers)

Start:
154,297,590 bp from pter
End:
154,306,919 bp from pter
Size:
9,330 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB25_HUMAN, P57735 (See protein sequence)
Recommended Name: Ras-related protein Rab-25  
Size: 213 amino acids; 23496 Da
Subunit: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential)
Sequence caution: Sequence=AAF98238.1; Type=Frameshift; Positions=196;
PDB structures from and Proteopedia :
2OIL (3D)    
Secondary accessions: Q5VYA2 Q8NG24 Q96GB1 Q9BT12

REFSEQ proteins: NP_065120.2  

ENSEMBL proteins: 
ENSP00000354376 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Rab25)
Human Recombinant Proteins from Abnova (RAB25)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IDA18029348
GO:0005886 plasma membrane IEA--
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Antibodies for RAB25: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for RAB25
Browse R&D Systems for Antibodies
Antibodies from Abcam (Rab25), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for RAB25

Assays for RAB25: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5 InterPro domains/families:
 IPR015595 Rab11
 IPR001806 Ras_GTPase
 IPR005225 Small_GTP_bd
 IPR003579 GTPase_Rab
 IPR013753 Ras


   GeneDecks  RAB25 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P57735

ProtoNet protein and cluster: P57735

4 Blocks protein families:
IPB001806 Transforming protein P21 RAS signature
IPB002041 GTP-binding nuclear protein Ran
IPB003577 Ras small GTPase
IPB003578 Ras small GTPase


UniProtKB/Swiss-Prot: RAB25_HUMAN, P57735
Similarity: Belongs to the small GTPase superfamily. Rab family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB25)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB25)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020387

              Applied Biosystems Silencer® siRNAs for RAB25

              Sigma-Aldrich siRNA for RAB25  
                     Sigma-Aldrich shRNA for RAB25  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for RAB25
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020387
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020387
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_020387 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_020387

UniProtKB/Swiss-Prot: RAB25_HUMAN, P57735
Function: May selectively regulate the apical recycling and/or transcytotic pathways (By
similarity)

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0005525 GTP binding IEA--
GO:0042802 identical protein binding IPI17353931
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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 Gene Network CentralTM Interacting Genes and Proteins Network for  RAB25 


3 Interacting proteins for RAB25 (P577351) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
D2HGDHQ8N4651EBI-1050500, EBI-1053783
PKIGQ9Y2B91EBI-1050500, EBI-1052231
RAB25P577351EBI-1050500, EBI-1050500
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007264 small GTPase mediated signal transduction IEA--
GO:0015031 protein transport IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Enzo Life Sciences drugs & compounds for RAB25
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for RAB25
1 Novoseek chemical compound relationship for RAB25 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
gtp 44.85 2 8360141 (2)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB25)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB25)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020387

              Sigma-Aldrich siRNA for RAB25  
                     Sigma-Aldrich shRNA for RAB25  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_020387  

REFSEQ mRNAs for RAB25 gene: 

NM_020387.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_020387  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020387
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020387
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_020387 

Additional cDNA sequence: 

AF083124.1 AF274025.1 BC004416.1 BC009831.2 BC033322.1 CR599507.1 

10 DOTS entries:

DT.317133  DT.86842442  DT.100792538  DT.97846953  DT.121330569  DT.92437040  DT.100792540  DT.97846954 
DT.100792542  DT.121330629 

24/198 AceView cDNA sequences (see all 198 ):

AI246699 CB128282 BM672100 BC004416 AL545490 BG682384 BM833326 BE718362 
BM762058 CA489548 BE745389 BE742686 BM834846 BQ933120 CB306287 BG680066 
BE272246 BM811535 CB160385 BM824143 BG292330 BM987996 BC009831 AA337947 

highest scoring ESTs for RAB25:

AA195079 AA130429 AA218694 AA403293 AA463354 AA463366 AA463861 AA463870 AA480665 AA507859 

Unigene Cluster for RAB25:

RAB25, member RAS oncogene family
Hs.632469  [show with all ESTs]
Unigene Representative Sequence: BE612887


GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for RAB25 (see all 6 )

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b
SP1:                          -     -                 -               
SP2:              -     -     -     -                 -               
SP3:                                                  -               
SP4:              -     -     -     -                                 
SP5:                    -     -     -                                 

About this scheme

ECgene alternative splicing isoforms for RAB25

1 Ensembl transcript including schematic representation:
ENST00000361084  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

RAB25 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RAB25

1 / 2 / 3

3 probe-sets matching RAB25 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
48963_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AA195079 1.00 1.00 1.00 1

218186_at2, 3 U133-A 1 1.00 1.00 -- -- NM_020387 0.60 1.00 0.82 1

218186_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  RAB25 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CCCTATCACA

SOURCE GeneReport for Unigene cluster: Hs.632469
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RAB25 gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RAB251   -- RAB25, member RAS oncogene family 88.27(n)
96.48(a)
490418  XM_547540.2  XP_547540.2 
chimpanzee
(Pan troglodytes)
RAB251   -- RAB25, member RAS oncogene family 99.84(n)
100(a)
457382  XM_001163533.1  XP_001163533.1 
cow
(Bos taurus)
RAB251   -- RAB25, member RAS oncogene family 91.39(n)
95.31(a)
506482  NM_001017936.1  NP_001017936.1 
rat
(Rattus norvegicus)
Rab251   -- RAB25, member RAS oncogene family 90.45(n)
96.71(a)
310632  XM_227404.4  XP_227404.1 
mouse
(Mus musculus)
Rab251, 5 35
RAB25, member RAS oncogene family1, 5 90.61(n)1
95.77(a)1
538681  NM_016899.31  NP_058595.21 
 AA7930545  AB2326205  (see all 10)
About this table        Species with no ortholog for RAB25

ENSEMBL Gene Tree for RAB25
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for RAB25 gene
RAB352  RAB11A2  RAB11B2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/42 NCBI SNPs in RAB25 are shown (see all 42 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 28)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs22750751,2
A,C,F,H154306934(+) ATTCCA/GCTGCC 1 -- ng3117Minor allele frequency- G:0.34EA WA NA CA CSAM EU 2990
rs22750731,2
A,C,F,H154297444(+) CCCCCC/AGCTGG 1 -- ng51 trp312Minor allele frequency- A:0.31EU EA WA NA 1192
rs22750741,2
H154304716(+) TCCTGG/ATGTTT 1 M/V mis1 ese33Minor allele frequency- A:0.00EA EU WA 1738
rs120491951,2
F,H154299511(+) gttggG/Agtccc 1 -- int13Minor allele frequency- A:0.17EA WA 298
rs120218901,2
H154299975(+) agggcG/Agggag 1 -- int14Minor allele frequency- A:0.00EU EA WA 416
--
rs46611451,2
A154303181(+) AAATCC/TTGTCT 1 -- int10--------
--
rs340228291,2
--154297019(+) TTTTT-/TTAGAC 1 -- ng510--------
--
rs113014121,2
--154297010(+) TTTTT-/TTTTTT 1 -- ng51 trp30--------
--
rs351105851,2
--154307385(+) AAGTCC/TTCCAT 1 -- ng310--------
--
rs721686861,2
--154297001(+) GCTAA-/TTTTTTT 1 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for RAB25 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/20 PubMed articles for RAB25 gene (see all 20 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 57111 HGNC: 18238 AceView: RAB25 Ensembl:ENSG00000132698 euGenes: HUgn57111
ECgene: RAB25 H-InvDB: RAB25
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for RAB25 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RAB25:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for RAB25-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for RAB25 Antibodies for RAB25
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for RAB25 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Drugs for RAB25
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for RAB25

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