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RAB21 Gene

protein-coding   GIFtS: 59

GC12P070434
RAB21, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
KIAA0118 1, 2, 3, 5
Descriptions
RAB21, member RAS oncogene family 2
External Ids
HGNC: 182631
Entrez Gene: 230112
UniProtKB: Q9UL253
Ensembl: ENSG000000803717
Search outside databases for aliases for RAB21 gene

Previous GC identifers: GC12P071929 GC12P072100

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for RAB21:
RAB21 belongs to the RAB family of small GTP-binding proteins that regulate intracellular vesicle
targeting (Opdam et al., 2000 [PubMed 10887961]).[supplied by OMIM]

UniProtKB/Swiss-Prot: RAB21_HUMAN, Q9UL25
Function: Regulates integrin internalization and recycling, but does not influence the traffic of
endosomally translocated receptors in general. As a result, may regulate cell adhesion and
migration (By similarity). During the mitosis of adherent cells, controls the endosomal
trafficking of integrins which is required for the successful completion of cytokinesis

Gene Wiki entry for RAB21

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB21 gene  

Entrez Gene cytogenetic band: 12q21.1   Ensembl cytogenetic band:  12q21.1   HGNC cytogenetic band: 12q15

RAB21 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P070434:     (about GC identifiers)

Start:
70,434,925 bp from pter
End:
70,467,417 bp from pter
Size:
32,493 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_029419.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB21_HUMAN, Q9UL25 (See protein sequence)
Recommended Name: Ras-related protein Rab-21  
Size: 225 amino acids; 24348 Da
Subunit: Interacts with the cytoplasmic tail of integrins ITGA1, ITGA2, ITGA5, ITGA6, ITGA11 and
ITGB1 (By similarity)
Subcellular location: Endoplasmic reticulum membrane; Lipid-anchor (By similarity). Golgi apparatus
membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Note=In nonpolarized epithelial
Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the
apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow.
In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base
of lamellipodia formed by the separating daughter cells
PDB structures from and Proteopedia :
1YZT (3D)    1YZU (3D)    1Z08 (3D)    1Z0I (3D)    2OT3 (3D)    
Secondary accessions: Q14466 Q569H3

REFSEQ proteins: NP_055814.1  

ENSEMBL proteins: 
ENSP00000261263 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (RAB21)
Human Recombinant Proteins from Abnova (RAB21)
              OriGene Purified Recombinant Human Protein: RAB21 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000139 Golgi membrane IEA--
GO:0005768 endosome IDA11256614
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
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Antibodies for RAB21: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for RAB21
Browse R&D Systems for Antibodies
Antibodies from Abcam (RAB21), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (RAB21)
Novus Biologicals Antibodies for RAB21

Assays for RAB21: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5 InterPro domains/families:
 IPR001806 Ras_GTPase
 IPR005225 Small_GTP_bd
 IPR003579 GTPase_Rab
 IPR013753 Ras
 IPR015601 Ras_Rab21-like


   GeneDecks  RAB21 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9UL25

ProtoNet protein and cluster: Q9UL25

1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

UniProtKB/Swiss-Prot: RAB21_HUMAN, Q9UL25
Similarity: Belongs to the small GTPase superfamily. Rab family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB21)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB21)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014999

              Applied Biosystems Silencer® siRNAs for RAB21

              Sigma-Aldrich siRNA and siRNA Panels for RAB21  
                     Sigma-Aldrich shRNA for RAB21  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014999
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014999
                                 untagged cDNA clone in CMV expression vector: NM_014999 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014999

UniProtKB/Swiss-Prot: RAB21_HUMAN, Q9UL25
Function: Regulates integrin internalization and recycling, but does not influence the traffic of
endosomally translocated receptors in general. As a result, may regulate cell adhesion and
migration (By similarity). During the mitosis of adherent cells, controls the endosomal
trafficking of integrins which is required for the successful completion of cytokinesis

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0005525 GTP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



3 Interacting proteins for RAB21 (Q9UL252 ENSP000002612633) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
APPL1Q9UKG12, ENSP000002882663MINT-5207865 MINT-5207834 MINT-5207897 MINT-5208028 MINT-5207964 MINT-5208076 MINT-5207996 MINT-5207916 MINT-5204063 MINT-5208044 MINT-5208060 STRING (score=.83)
ANKRD27ENSP000003042923STRING (score=.949)
ZNF749ENSP000003702103STRING (score=.847)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007264 small GTPase mediated signal transduction IEA--
GO:0015031 protein transport IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for RAB21
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for RAB21

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (RAB21)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (RAB21)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014999

              Sigma-Aldrich siRNA and siRNA Panels for RAB21  
                     Sigma-Aldrich shRNA for RAB21  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_014999  

REFSEQ mRNAs for RAB21 gene: 

NM_014999.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_014999  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014999
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014999
                                 untagged cDNA clone in CMV expression vector: NM_014999 

Additional cDNA sequence: 

AB075482.1 AF091035.1 BC009109.1 BC021901.1 BC092475.1 CR590140.1 CR600765.1 CR604873.1 
D42087.1 

9 DOTS entries:

DT.209481  DT.91922497  DT.97861767  DT.100826817  DT.65284362  DT.100680561  DT.75102206  DT.100826811 
DT.121206658 

24/221 AceView cDNA sequences (see all 221 ):

BX119716 BM893579 BM978770 CD722746 D42087 CA314817 BG771050 BQ437436 
BE501894 BP382887 CA866159 AA922618 BX117131 BQ686309 BF063415 BX499957 
CK430593 CR600765 BM471710 AI025203 CR590140 AI274214 BF060662 CA941131 

highest scoring ESTs for RAB21:

D42087 AA581270 AF091035 AI025203 AI189956 AI219379 AI471033 AL041855 AL526366 BC009109 

Unigene Cluster for RAB21:

RAB21, member RAS oncogene family
Hs.524590  [show with all ESTs]
Unigene Representative Sequence: BC009109


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000261263  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

RAB21 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for RAB21

1 / 2 / 3

3 probe-sets matching RAB21 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
33326_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D42087 1.00 1.00 1.00 1

203885_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014999 0.60 1.00 0.82 1

203885_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  RAB21 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TAATGCAGAT

SOURCE GeneReport for Unigene cluster: Hs.524590

Expression variation in blood from EXPOLDB for RAB21

UniProtKB/Swiss-Prot: RAB21_HUMAN, Q9UL25
Tissue specificity: Widely expressed. In jejunal tissue, predominantly expressed in the apical
region of the epithelial cell layer of the villi, weak expression, if any, in the crypt
epithelium. Capillary endothelium and some cell types in the lamina propria also show expression

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for RAB21 gene from 5/12 species (see all 12 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
RAB211   -- RAB21, member RAS oncogene family 99.4(n)
99.55(a)
467069  XM_522469.2  XP_522469.2 
cow
(Bos taurus)
RAB211   -- RAB21, member RAS oncogene family 95.35(n)
97.75(a)
507488  NM_001075317.1  NP_001068785.1 
rat
(Rattus norvegicus)
Rab211   -- RAB21, member RAS oncogene family 90.84(n)
95.95(a)
299799  NM_001004238.1  NP_001004238.1 
mouse
(Mus musculus)
Rab211, 5 105
RAB21, member RAS oncogene family1, 5 90.2(n)1
96.83(a)1
2163441  NM_024454.11  NP_077774.11 
 AB2326155  AI8540335  (see all 10)
chicken
(Gallus gallus)
RAB211   -- RAB21, member RAS oncogene family 86.79(n)
95.39(a)
771383  XM_001233647.1  XP_001233648.1 
About this table        Species with no ortholog for RAB21

ENSEMBL Gene Tree for RAB21
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for RAB21 gene
RAB202  RAB6C2  RAB412  RAB6A2  RAB6B2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/150 NCBI SNPs in RAB21 are shown (see all 150 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 88)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 12 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs3287421,2
A,C,F,H,O70466439(-) AATTTA/CAACTA 1 -- ut3117Minor allele frequency- N:0.00MN EU EA WA NA 1646
rs3287431,2
A,C,F,H70467348(-) CTACTA/CAAATT 1 -- ut31 ese35Minor allele frequency- C:0.07EU WA NA 378
rs22773751,2
A,C,F,H70434562(-) TCTCTG/CTCTGT 1 -- ng518Minor allele frequency- C:0.45EU EA WA NA 780
rs3287411,2
C,F,H70465722(-) TGAACA/GGTTAT 1 -- ut319Minor allele frequency- G:0.04MN EU EA WA 1022
rs123107991,2
C,F,H70467573(+) ATTATA/GGTTAC 1 -- ng314Minor allele frequency- G:0.23EU EA WA 390
rs111789441,2
F,H70465881(+) TGCTAA/TACTTA 1 -- ut314Minor allele frequency- T:0.00EU EA WA 414
--
rs108793301,2
A,C70434227(+) GAGGCC/TTCATG 1 -- ng510--------
rs171102781,2
F,H70467002(+) TTATAA/GGAGAT 1 -- ut31 ese37Minor allele frequency- G:0.06NA EU EA WA 562
--
rs111789301,2
A70433507(+) AAGCGC/TTTCCC 1 -- ng510--------
--
rs108793311,2
C70434615(+) TGTGAC/GAGATC 1 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for RAB21 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 612398

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/13 PubMed articles for RAB21 gene (see all 13 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 23011 HGNC: 18263 AceView: RAB21 Ensembl:ENSG00000080371 euGenes: HUgn23011
ECgene: RAB21 H-InvDB: RAB21
(According to HUGE)
About This Section

HUGE: KIAA0118
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for RAB21 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for RAB21:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Millipore Preclinical / Clinical Development Services
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 Predesigned and custom siRNAs for RAB21 Antibodies for RAB21
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for RAB21 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Custom Service for Rabbit Pab from Full-length Protein
  
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 Recombinant Proteins
(RAB21)
 Antibodies (RAB21)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for RAB21




 Drugs for RAB21
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for RAB21

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