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RAB1B Gene

protein-coding   GIFtS: 60

GC11P065777
RAB1B, member RAS oncogene family
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Descriptions
RAB1B, member RAS oncogene family 2
small GTP-binding protein 2
External Ids
HGNC: 183701
Entrez Gene: 818762
UniProtKB: Q9H0U43
Ensembl: ENSG000001749037
Search outside databases for aliases for RAB1B gene

Previous GC identifers: GC11P068570 GC11P067717 GC11P066285 GC11P065811 GC11P065792

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for RAB1B:
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases
localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in
the early secretory pathway and is essential for vesicle transport between the endoplasmic
reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed
12802079]).[supplied by OMIM]

UniProtKB/Swiss-Prot: RAB1B_HUMAN, Q9H0U4
Function: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and
successive Golgi compartments

Gene Wiki entry for RAB1B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the RAB1B gene  

Entrez Gene cytogenetic band: 11q12   Ensembl cytogenetic band:  11q13.1   HGNC cytogenetic band: 11q13.1

RAB1B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P065777:     (about GC identifiers)

Start:
65,777,735 bp from pter
End:
65,801,539 bp from pter
Size:
23,805 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000011.8  NT_033903.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RAB1B_HUMAN, Q9H0U4 (See protein sequence)
Recommended Name: Ras-related protein Rab-1B  
Size: 201 amino acids; 22171 Da
Subunit: Interacts with MICAL1, MICAL2 and MICAL3. Interacts with GDI1; the interaction requires
the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is
necessary for prenylation by GGTase II
Subcellular location: Membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm. Note=Targeted by REP1 to
membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus,
and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in
the cytoplasm (By similarity)
Miscellaneous: Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity (By similarity)

Post-translational modifications:

  • Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_112243.1  

    ENSEMBL proteins: 
    ENSP00000313814 ENSP00000310226 ENSP00000377642 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for RAB1B  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (RAB1B)
    Human Recombinant Proteins from Abnova (RAB1B)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0005794 Golgi apparatus IDA11256614
    GO:0016020 membrane IEA--
    About this table

    Antibodies for RAB1B: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for RAB1B
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (RAB1B), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (RAB1B)
    Novus Biologicals Antibodies for RAB1B

    Assays for RAB1B: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR001806 Ras_GTPase
     IPR005225 Small_GTP_bd
     IPR003579 GTPase_Rab
     IPR013753 Ras


       GeneDecks  RAB1B for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H0U4

    ProtoNet protein and cluster: Q9H0U4

    1 Blocks protein family: IPB001806 Transforming protein P21 RAS signature

    UniProtKB/Swiss-Prot: RAB1B_HUMAN, Q9H0U4
    Similarity: Belongs to the small GTPase superfamily. Rab family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB1B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB1B)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030981

                  Applied Biosystems Silencer® siRNAs for RAB1B

                  Sigma-Aldrich siRNA for RAB1B  
                         Sigma-Aldrich shRNA for RAB1B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for RAB1B
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030981
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030981
                                     untagged cDNA clone in CMV expression vector: NM_030981 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_030981

    UniProtKB/Swiss-Prot: RAB1B_HUMAN, Q9H0U4
    Function: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and
    successive Golgi compartments

    1 MGI phenotypic allele for Rab1b (no phenotypes)


    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0005515 protein binding IPI17353931
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  RAB1B 


    5/7 Interacting proteins for RAB1B (Q9H0U41, 2 ENSP000003102263) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RUSC2Q8N2Y82, ENSP000003551773MINT-61270 MINT-61269 MINT-61266 STRING (score=.961)
    MICAL1Q8TDZ22, ENSP000003516643MINT-61264 STRING (score=.829)
    VHLP403371EBI-1045214, EBI-301246
    GOLGA2Q083792MINT-61265
    CHMENSP000003503863STRING (score=.797)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0015031 protein transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for RAB1B
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for RAB1B
    2 Novoseek chemical compound relationships for RAB1B gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    gdp 67.66 3 8325834 (1), 9437002 (1), 8836150 (1)
    gtp 57.15 1 8325834 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (RAB1B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (RAB1B)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030981

                  Sigma-Aldrich siRNA for RAB1B  
                         Sigma-Aldrich shRNA for RAB1B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_030981  

    REFSEQ mRNAs for RAB1B gene: 

    NM_030981.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_030981  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030981
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030981
                                     untagged cDNA clone in CMV expression vector: NM_030981 

    Additional cDNA sequence: 

    AJ245875.1 AK125412.1 AK292086.1 AK315333.1 AL136635.1 BC028331.1 BC071169.1 CR533462.1 
    CR591273.1 CR592347.1 CR594164.1 CR595400.1 CR598883.1 CR599415.1 CR602885.1 CR605155.1 
    CR612971.1 CR620997.1 CR624810.1 CR625419.1 U66621.1 

    24/30 DOTS entries (see all 30 ):

    DT.100786824  DT.91908062  DT.100757341  DT.121192845  DT.99998569  DT.120735097  DT.120735063  DT.216580 
    DT.92041333  DT.99985353  DT.91652303  DT.100786834  DT.91867251  DT.40256768  DT.92450775  DT.95177030 
    DT.97864437  DT.100770591  DT.100786826  DT.100786831  DT.120735110  DT.91695929  DT.91933592  DT.92450787 

    24/705 AceView cDNA sequences (see all 705 ):

    AW733009 BM922803 BP372300 BM701329 BM811149 AL558136 CR625790 BM785457 
    BI771821 AL552559 CA415649 BM703149 BU162772 BQ722819 BI821119 BM703546 
    CA393447 BE882786 AI350693 AL136864 BM466138 AA368694 CR592347 BQ718215 

    highest scoring ESTs for RAB1B:

    AA076613 AA284973 AA293334 AA327651 AA350769 AA350770 AA365610 AA401715 AA424721 AA434038 

    Unigene Cluster for RAB1B:

    RAB1B, member RAS oncogene family
    Hs.300816  [show with all ESTs]
    Unigene Representative Sequence: AK125412


    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for RAB1B

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b
    SP1:                                          
    SP2:                    -                     

    About this scheme

    ECgene alternative splicing isoforms for RAB1B

    3 Ensembl transcripts including schematic representations:
    ENST00000314965  ENST00000311481  ENST00000394080  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    RAB1B expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for RAB1B

    1 / 2 / 3

    5 probe-sets matching RAB1B gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    43390_s_at2, 3 U95-B 1 1.00 1.00 0.61 1.25 R42995 0.80 1.00 0.91 1

    65903_at2, 3 U95-C 1 1.00 1.00 0.91 1.04 W30716 0.80 1.00 0.91 1
    65902_at2, 3 U95-C 2 1.00 0.75 0.96 0.71 AI082241 0.80 1.00 0.91 1

    220964_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_030981 0.60 1.00 0.82 1

    220964_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  RAB1B for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TCAGGCATTT

    SOURCE GeneReport for Unigene cluster: Hs.300816
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for RAB1B gene from 5/16 species (see all 16 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RAB1B1   -- RAB1B, member RAS oncogene family 93.7(n)
    100(a)
    610762  XM_847452.1  XP_852545.1 
    chimpanzee
    (Pan troglodytes)
    RAB1B1   -- RAB1B, member RAS oncogene family 99.67(n)
    99.5(a)
    745036  XM_001150399.1  XP_001150399.1 
    rat
    (Rattus norvegicus)
    Rab1b-ps11   -- RAB1B, member RAS oncogene family, pseudogene 1 90.38(n)
    98.51(a)
    361706  NM_001008370.1  NP_001008371.1 
    mouse
    (Mus musculus)
    Rab1b1, 5 195
    RAB1B, member RAS oncogene family1, 5 91.21(n)1
    99(a)1
    763081  NM_029576.21  NP_083852.11 
     AB2325845  AK0036095  (see all 15)
    zebrafish
    (Danio rerio)
    zgc560492   -- similar to RAB1, member RAS oncogene family 81.37(n) 394117  BC047816.1 
    About this table        Species with no ortholog for RAB1B

    ENSEMBL Gene Tree for RAB1B
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for RAB1B gene
    RAB1A2  RAB132  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/44 NCBI SNPs in RAB1B are shown (see all 44 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 27)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs38147391,2
    C,F,H65792555(-) TCTCCA/GACTCA 1 -- ng517Minor allele frequency- G:0.35EA MN EU WA 2294
    rs5248591,2
    A,C,F,H65797655(+) GCATGG/ACTGCG 1 -- int19Minor allele frequency- A:0.13EU EA WA 1010
    rs11515401,2
    A,C,F,H65798383(-) GAGAAT/GGAACA 1 -- int17Minor allele frequency- G:0.29EU EA WA NA 536
    rs22984681,2
    C,F,H65799538(-) AACCAC/TATGTG 1 -- int113Minor allele frequency- N:0.00EA NA EU WA 2488
    --
    rs107507811,2
    A,C65793846(+) AGATGA/GTGCAT 1 -- int10--------
    --
    rs20179691,2
    A,C65795608(+) gctacA/Gcagga 1 -- int10--------
    --
    rs5544591,2
    C65793895(+) CTCAAG/TTATAT 1 -- int10--------
    --
    rs5558261,2
    C65796629(-) TCCTGC/TAAGCA 1 -- int10--------
    --
    rs5558531,2
    A65796625(-) GTAAGC/GAGACA 1 -- int10--------
    --
    rs115553851,2
    --65796487(+) TCGTGG/ATGTAT 1 M/V mis11Minor allele frequency- A:0.00EA 170
    About this table

    HapMap Linkage Disequilibrium images for RAB1B (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 612565

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/28 PubMed articles for RAB1B gene (see all 28 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 81876 HGNC: 18370 AceView: KLC2andRAB1B Ensembl:ENSG00000174903 euGenes: HUgn81876
    ECgene: RAB1B H-InvDB: RAB1B
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for RAB1B Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for RAB1B:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Lentivirus-delivered shRNAs for RAB1B Browse small molecules at Sigma-Aldrich
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     Drugs for RAB1B
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for RAB1B

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