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R3HDML Gene

protein-coding GIFtS: 41

GC20P042399

R3H domain containing-like
(Previous name: R3H domain (binds single-stranded nucleic acids) containing-like )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
MGC129564 ^²
OTTHUMP00000031047 ^²
dJ881L22.3 ^¹, ²

Descriptions
Cysteine-rich secretory protein R3HDML ^³
R3H domain (binds single-stranded nucleic acids) containing-like ^¹, ²
R3H domain containing-like ^²

External Ids
HGNC: 16249¹
Entrez Gene: 140902²
UniProtKB: Q9H3Y0³
Ensembl: ENSG00000101074⁷

Search outside databases for aliases for R3HDML gene

Previous GC identifers: GC20U990180 GC20M042654 GC20P043605 GC20P043651

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: CRSPL_HUMAN, Q9H3Y0

Function: Putative serine protease inhibitor (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the R3HDML gene

Entrez Gene cytogenetic band: 20q13.12 Ensembl cytogenetic band: 20q13.12 HGNC cytogenetic band: 20q13.12

R3HDML Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 20 GeneLoc Exon Structure

GeneLoc location for GC20P042399: (about GC identifiers)

Start:	42,399,040 bp from pter
End:	42,413,289 bp from pter
Size:	14,250 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000020.9 NT_011362.9

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CRSPL_HUMAN, Q9H3Y0 (See protein sequence)

Recommended Name: Peptidase inhibitor R3HDML precursor

Size: 253 amino acids; 28605 Da

Subcellular location: Secreted (Potential)

Caution: Despite its name, it does not contain a R3H domain

REFSEQ proteins: NP_848586.1

ENSEMBL proteins:

ENSP00000217043

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (R3HDML)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--

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Antibodies for R3HDML:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for R3HDML

Assays for R3HDML:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q9H3Y0

ProtoNet protein and cluster: Q9H3Y0

1 Blocks protein family: IPB001283 Allergen V5/Tpx-1 related

UniProtKB/Swiss-Prot: CRSPL_HUMAN, Q9H3Y0

Similarity: Belongs to the CRISP family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (R3HDML)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_178491

Applied Biosystems Silencer® siRNAs for R3HDML

Sigma-Aldrich siRNA for R3HDML

Sigma-Aldrich shRNA for R3HDML

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_178491
Myc/DDK tagged cDNA clone in CMV expression vector: NM_178491
untagged cDNA clone in CMV expression vector: NM_178491

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_178491

UniProtKB/Swiss-Prot: CRSPL_HUMAN, Q9H3Y0

Function: Putative serine protease inhibitor (By similarity)

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004866	endopeptidase inhibitor activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for R3HDML

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (R3HDML)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_178491

Sigma-Aldrich siRNA for R3HDML

Sigma-Aldrich shRNA for R3HDML

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_178491

REFSEQ mRNAs for R3HDML gene:

NM_178491.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_178491

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_178491
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_178491
                                 untagged cDNA clone in CMV expression vector: NM_178491

Additional cDNA sequence: BC107048.2

1 DOTS entry:

DT.92045296

2 AceView cDNA sequences:

NM_178491 BG221330

highest scoring ESTs for R3HDML:

BG221330 NM_178491 BG203625 AW062673 AW140137 BF335408 BF335440 BF335731 BF758013 BG961652

Unigene Cluster for R3HDML:

R3H domain containing-like

Hs.580807 [show with all ESTs]

Unigene Representative Sequence: NM_178491

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000217043

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

R3HDML expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for R3HDML

¹ / ²

3 probe-sets matching R3HDML gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
234774_at²	U133-B	1	1.00	1.00	--	--	--	--	--	--	--
234774_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
1559788_at*²	U133Plus2	1	0.91	1.00	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GGTCAAAAGA

SOURCE GeneReport for Unigene cluster: Hs.580807

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for R3HDML gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	R3HDML¹	--	R3H domain containing-like	86.64(n) 85.32(a)	485883 XM_543007.2 XP_543007.2
chimpanzee (Pan troglodytes)	R3HDML¹	--	R3H domain containing-like	99.21(n) 98.81(a)	469949 XM_525333.2 XP_525333.1
cow (Bos taurus)	R3HDML¹	--	R3H domain containing-like	85.19(n) 81.75(a)	533734 XM_613233.3 XP_613233.3
mouse (Mus musculus)	R3hdml⁵ OTTMUSG00000001070¹	2⁵	R3H domain containing-like⁵ predicted gene, OTTMUSG00000001070¹	81.61(n)¹ 78.17(a)¹	100043899¹ XM_001481189.1¹ XP_001481239.1¹ AL591488⁵

About this table Species with no ortholog for R3HDML

ENSEMBL Gene Tree for R3HDML

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for R3HDML gene

CRISPLD2² CRISPLD1² PI16² PI15²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/133 NCBI SNPs in R3HDML are shown (see all 133 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 85)

AB	SNP ID	Valid	Chr 20 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs4812828^1,2	A,C,F,H	42413734(+)	`GGCAGC/TCTTTG`	1	--	ng3¹		8		EU EA WA NA	608
	rs2868093^1,2	C,F,H	42397212(+)	`agtaaC/Tgtgcc`	1	--	ng5¹		12		EU EA WA	1258
	rs11905257^1,2	C,F,H	42397834(+)	`taaatC/Tctggc`	1	--	ng5¹		4		EU EA WA	420
	rs11699901^1,2	C,F,H	42399255(+)	`GCTCTT/GCTGGG`	1	C/F	mis¹ ese³		7		EA WA NA	588
	rs12624485^1,2	C,F,H	42397928(+)	`ctataG/Atacct`	1	--	ng5¹		4		EU EA WA	420
	rs11699154^1,2	C,F,H	42412858(+)	`TCTGGG/ACTTTG`	1	--	ut3¹		4		EU EA WA	420
	rs11699841^1,2	C,F,H	42398855(+)	`CAGGAT/CTGGTG`	1	--	ng5¹		4		EU EA WA	420
	rs11699172^1,2	C,F,H	42412967(+)	`CTGTTG/AAATTT`	1	--	ut3¹		4		EU EA WA	418
	rs36117710^1,2	C,F	42399413(-)	`ATAATC/TCAGTA`	1	D/N	mis¹		5		NA EU EA WA	492
--	rs11697761^1,2	C	42413293(+)	`acaaaC/TaaaAA`	1	--	ng3¹		0	--	--	--	--

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HapMap Linkage Disequilibrium images for R3HDML (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

3 PubMed articles for R3HDML gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)⁴ Deloukas P....Rogers J. (2001)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 140902	HGNC: 16249	AceView: R3HDML	Ensembl:ENSG00000101074	euGenes: HUgn140902
ECgene: R3HDML	H-InvDB: R3HDML

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for R3HDML
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for R3HDML:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for R3HDML-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for R3HDML		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for R3HDML		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (R3HDML)
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	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

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Search OriGene for R3HDML

Search Tocris compounds for R3HDML

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for R3HDML

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R3HDML Gene

protein-coding GIFtS: 41

Aliases &
Descriptions
for R3HDML

Summaries
for R3HDML

Genomic Location
for R3HDML

Proteins
for R3HDML

Protein Domains/
Families
for R3HDML

Gene Function
for R3HDML

Pathways & Interactions
for R3HDML

Drugs & Compounds
for R3HDML

Transcripts
for R3HDML

Expression
for R3HDML

Orthologs
for R3HDML

Paralogs
for R3HDML

SNPs/Variants
for R3HDML

Disorders & Mutations
for R3HDML

Medical News
for R3HDML

Publications
for R3HDML

Search for R3HDML

Genome Databases showing R3HDML

Other Databases showing R3HDML

Specialized Databases showing R3HDML

Licensable Technologies
for R3HDML

Services
for R3HDML

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

R3HDML Gene

protein-coding GIFtS: 41

Aliases & Descriptionsfor R3HDML

Summariesfor R3HDML

Genomic Locationfor R3HDML

Proteinsfor R3HDML

Protein Domains/Familiesfor R3HDML

Gene Functionfor R3HDML

Pathways & Interactionsfor R3HDML

Drugs & Compoundsfor R3HDML

Transcriptsfor R3HDML

Expression for R3HDML

Orthologsfor R3HDML

Paralogsfor R3HDML

SNPs/Variantsfor R3HDML

Disorders & Mutationsfor R3HDML

Medical Newsfor R3HDML

Publicationsfor R3HDML

Search for R3HDML

Genome Databases showing R3HDML

Other Databases showing R3HDML

Specialized Databases showing R3HDML

Licensable Technologiesfor R3HDML

Servicesfor R3HDML

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for R3HDML

Summaries
for R3HDML

Genomic Location
for R3HDML

Proteins
for R3HDML

Protein Domains/
Families
for R3HDML

Gene Function
for R3HDML

Pathways & Interactions
for R3HDML

Drugs & Compounds
for R3HDML

Transcripts
for R3HDML

Expression
for R3HDML

Orthologs
for R3HDML

Paralogs
for R3HDML

SNPs/Variants
for R3HDML

Disorders & Mutations
for R3HDML

Medical News
for R3HDML

Publications
for R3HDML

Licensable Technologies
for R3HDML

Services
for R3HDML