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QTRT1 Gene

protein-coding   GIFtS: 49

GC19P010676
queuine tRNA-ribosyltransferase 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 2.4.2.29 3
FP3235 2
TGT 1, 2, 3, 5
TGUT 3
Descriptions
Guanine insertion enzyme 3
queuine tRNA-ribosyltransferase 1 2
tRNA-guanine transglycosylase 2, 3
External Ids
HGNC: 237971
Entrez Gene: 818902
UniProtKB: Q9BXR03
Ensembl: ENSG000002133397
Search outside databases for aliases for QTRT1 gene

Previous GC identifers: GC19P010674 GC19P010675

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for QTRT1:
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs
that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single
protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD
subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001
[PubMed 11255023]).[supplied by OMIM]

UniProtKB/Swiss-Prot: TGT_HUMAN, Q9BXR0
Function: Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N)
anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is
attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside
queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the QTRT1 gene  

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.2   HGNC cytogenetic band: 19p13.3

QTRT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P010676:     (about GC identifiers)

Start:
10,673,131 bp from pter
End:
10,685,040 bp from pter
Size:
11,910 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011295.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TGT_HUMAN, Q9BXR0 (See protein sequence)
Recommended Name: Queuine tRNA-ribosyltransferase  
Size: 403 amino acids; 44048 Da
Cofactor: Binds 1 zinc ion per subunit (By similarity)
Secondary accessions: Q96BQ4 Q9BXQ9

REFSEQ proteins: NP_112486.1  

ENSEMBL proteins: 
ENSP00000250237 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (QTRT1)
Human Recombinant Proteins from Abnova (QTRT1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005840 ribosome NAS--
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Antibodies for QTRT1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for QTRT1
Browse R&D Systems for Antibodies
Antibodies from Abcam (QTRT1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (QTRT1)
Novus Biologicals Antibody for QTRT1

Assays for QTRT1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR002616 tRNA_ribo_trans
 IPR004803 QtRNA_ribo_trans


   GeneDecks  QTRT1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9BXR0

ProtoNet protein and cluster: Q9BXR0

1 Blocks protein family: IPB002616 Queuine/other tRNA-ribosyltransferase

UniProtKB/Swiss-Prot: TGT_HUMAN, Q9BXR0
Similarity: Belongs to the queuine tRNA-ribosyltransferase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (QTRT1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_031209

              Applied Biosystems Silencer® siRNAs for QTRT1

              Sigma-Aldrich siRNA for QTRT1  
                     Sigma-Aldrich shRNA for QTRT1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for QTRT1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_031209
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_031209
                                 untagged cDNA clone in CMV expression vector: NM_031209 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_031209

UniProtKB/Swiss-Prot: TGT_HUMAN, Q9BXR0
Function: Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N)
anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is
attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside
queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
Catalytic activity: [tRNA]-guanine + queuine = [tRNA]-queuine + guanine
Catalytic activity: [tRNA]-guanine + 7-aminomethyl-7-carbaguanine =
[tRNA]-7-aminomethyl-7-carbaguanine + guanine
Enzyme Number (IUBMB): EC 2.4.2.29 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008270 zinc ion binding IEA--
GO:0008479 queuine tRNA-ribosyltransferase activity TAS11255023
GO:0016757 transferase activity, transferring glycosyl groups IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008616 queuosine biosynthetic process NAS11255023
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for QTRT1
7 Novoseek chemical compound relationships for QTRT1 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
queuine 95.52 6 12877882 (1), 7630726 (1), 11255023 (1), 14602484 (1) (see all 5)
trna guanine 95.11 2 7630726 (1), 12581659 (1)
7-deazaguanine 86.97 1 9369241 (1)
trna 72.55 12 1378304 (2), 11134952 (2), 16206323 (1), 7630726 (1) (see all 5)
anticodon 59.51 1 7630726 (1)
guanosine 49.48 2 15246567 (1)
nucleoside 45.00 4 11134952 (1), 15246567 (1)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (QTRT1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_031209

              Sigma-Aldrich siRNA for QTRT1  
                     Sigma-Aldrich shRNA for QTRT1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

REFSEQ mRNAs for QTRT1 gene: 

NM_031209.2   


              OriGene GFP tagged cDNA clone in CMV expression vector: NM_031209
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_031209
                                 untagged cDNA clone in CMV expression vector: NM_031209 

Additional cDNA sequence: 

AF302783.1 AF302784.1 AF302785.1 AF370379.1 AK055216.1 AK057877.1 AK225173.1 AK294166.1 
AK314308.1 BC015350.1 CR602960.1 CR614822.1 CR614899.1 

20 DOTS entries:

DT.100027084  DT.121465724  DT.442457  DT.100699569  DT.100652329  DT.121465619  DT.121465708  DT.100027083 
DT.100796387  DT.100796386  DT.40316301  DT.97845567  DT.121465747  DT.97798801  DT.121465660  DT.121465665 
DT.121465685  DT.121465738  DT.121465706  DT.91997349 

24/186 AceView cDNA sequences (see all 186 ):

CR602960 BC015350 BM756341 CB164816 AI492206 BQ929911 AL138462 BU508404 
BQ894940 BF939611 AI366116 BF447204 CR614822 BM747043 BM052911 BM757226 
BM719390 BM511883 BM460709 AL536012 BU621033 BU634015 AL138461 BU629648 

highest scoring ESTs for QTRT1:

AA514559 AA649522 AA827574 AF302783 AF302784 AI015010 AI366116 AI433680 AI492206 AI571124 

Unigene Cluster for QTRT1:

Queuine tRNA-ribosyltransferase 1
Hs.631638  [show with all ESTs]
Unigene Representative Sequence: BM467261


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000250237  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

QTRT1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for QTRT1

1 / 2 / 3

3 probe-sets matching QTRT1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

78349_at2, 3 U95-E 1 1.00 1.00 1.00 1.00 AA514559 0.80 1.00 0.91 1

221270_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_031209 0.60 1.00 0.82 1

221270_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TACGGGGATC

SOURCE GeneReport for Unigene cluster: Hs.631638
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for QTRT1 gene from 5/17 species (see all 17 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
QTRT11   -- queuine tRNA-ribosyltransferase 1 89.72(n)
94.78(a)
476711  XM_848922.1  XP_854015.1 
chimpanzee
(Pan troglodytes)
QTRT11   -- queuine tRNA-ribosyltransferase 1 99.67(n)
100(a)
455717  XM_512381.2  XP_512381.1 
cow
(Bos taurus)
QTRT11   -- queuine tRNA-ribosyltransferase 1 87.56(n)
92.67(a)
505497  XM_581789.3  XP_581789.2 
rat
(Rattus norvegicus)
Qtrt11   -- queuine tRNA-ribosyltransferase 1 84.01(n)
89.83(a)
64016  NM_022250.1  NP_071586.1 
mouse
(Mus musculus)
Qtrt11, 5 95
queuine tRNA-ribosyltransferase 11, 5 84.37(n)1
88.34(a)1
605071  NM_021888.11  NP_068688.11 
 AB0346325  AI3271275  (see all 12)
About this table        Species with no ortholog for QTRT1

ENSEMBL Gene Tree for QTRT1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for QTRT1 gene
QTRTD12  

Pseudogenes
1 related pseudogene


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/54 NCBI SNPs in QTRT1 are shown (see all 54 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 12)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 19 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs48045201,2
A,C10672667(+) cactgC/Tgcccg 1 -- ng510--------
--
rs115422881,2
H10673263(+) CCACTC/ACTGTG 1 T/P mis14Minor allele frequency- A:0.00EU EA WA 404
rs116724311,2
C,F,H10684940(+) ACACTG/TGGCTG 1 L/L syn14Minor allele frequency- T:0.06EU EA WA 420
rs8920851,2
A,C,F,H,O10679092(-) TCCCAC/TCACAT 1 -- int116Minor allele frequency- T:0.37EA MN EU WA NA 3120
rs44250061,2
A,C,F,H10674364(+) AAATAC/TCTGTT 1 -- int111Minor allele frequency- T:0.15EU EA WA NA 982
rs72516931,2
A,C,F,H10675081(+) AGGGTC/GAAAAC 1 -- int110Minor allele frequency- G:0.07EU EA WA NA 1016
rs110857441,2
C,F,H10680967(+) CAGAGC/TGAAAG 1 -- int14Minor allele frequency- T:0.46EU EA WA 414
rs176982151,2
C,F,H10680175(+) CCGTGC/TGTCAT 1 -- int116Minor allele frequency- T:0.17NA EU EA WA 1460
--
rs116719681,2
A,C10679873(+) tgaccG/Ttgtga 1 -- int10--------
--
rs65117091,2
A,C10683278(+) gaccaA/Caggcg 1 -- int10--------
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HapMap Linkage Disequilibrium images for QTRT1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 609615

1 Novoseek disease relationship for QTRT1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
cancer 0.00 2 11134952 (1), 15246567 (1)
About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/25 PubMed articles for QTRT1 gene (see all 25 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 81890 HGNC: 23797 AceView: QTRT1 Ensembl:ENSG00000213339 euGenes: HUgn81890
ECgene: QTRT1 H-InvDB: QTRT1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for QTRT1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for QTRT1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for QTRT1 Antibodies for QTRT1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for QTRT1 Browse small molecules at Sigma-Aldrich
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for QTRT1

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