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QSOX1 Gene

protein-coding   GIFtS: 49

GC01P178391
quiescin Q6 sulfhydryl oxidase 1
(Previous name: quiescin Q6 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: QSCN6)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 1.8.3.2 3
FLJ34858 2
OTTHUMP00000033189 2
OTTHUMP00000039111 2
Q6 2
QSCN6 2, 3
hQSOX 3
Descriptions
Quiescin Q6 1, 3
quiescin Q6 sulfhydryl oxidase 1 2
External Ids
HGNC: 97561
Entrez Gene: 57682
UniProtKB: O003913
Ensembl: ENSG000001162607
Search outside databases for aliases for QSOX1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for QSOX1:
This gene encodes a protein that contains domains of thioredoxin and ERV1, members of two
long-standing gene families. The gene expression is induced as fibroblasts begin to exit the
proliferative cycle and enter quiescence, suggesting that this gene plays an important role in
growth regulation. Two transcript variants encoding two different isoforms have been found for
this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: QSOX1_HUMAN, O00391
Function: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides
with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a
variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being
involved in growth regulation

Gene Wiki entry for QSOX1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q24   Ensembl cytogenetic band:  1q25.2   HGNC cytogenetic band: 1q24

QSOX1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P178391:     (about GC identifiers)

Start:
178,390,591 bp from pter
End:
178,447,290 bp from pter
Size:
56,700 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: QSOX1_HUMAN, O00391 (See protein sequence)
Recommended Name: Sulfhydryl oxidase 1 precursor  
Size: 747 amino acids; 82578 Da
Cofactor: Binds 1 FAD per subunit (By similarity)
Subunit: Monomer (By similarity)
Subcellular location: Isoform 1: Golgi apparatus membrane; Single-pass membrane protein
Subcellular location: Isoform 2: Secreted, extracellular space. Note=Found in the extracellular
medium of quiescent cells but is not found in proliferating cells
Miscellaneous: 'Quiescin Q6' means that it was the sixth clone to be found at a higher level of
expression in quiescent fibroblasts
Secondary accessions: Q59G29 Q5T2X0 Q8TDL6 Q8WVP4
Alternative splicing: 2 isoforms:  O00391-1   O00391-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001004128.1  NP_002817.2  


    ENSEMBL proteins: 
    ENSP00000375885 ENSP00000375883 ENSP00000356572 ENSP00000356574 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Quiescin Q6)
    Human Recombinant Proteins from Abnova (QSCN6)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/7 Gene Ontology (GO) cellular component terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane IEA--
    GO:0005576 extracellular region IEA--
    GO:0005615 extracellular space IDA10708601
    GO:0005794 Golgi apparatus IEA--
    GO:0016020 membrane IEA--
    About this table

    Antibodies for QSOX1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Quiescin Q6), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for QSOX1

    Assays for QSOX1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR017905 ERV/ALR_sulphydryl_oxidase
     IPR012335 Thioredoxin_fold
     IPR006863 Evr1_Alr
     IPR013766 Thioredoxin_domain
     IPR017936 Thioredoxin-like


       GeneDecks  QSOX1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O00391

    ProtoNet protein and cluster: O00391

    2 Blocks protein families:
    IPB006662 Thioredoxin type domain
    IPB006863 Erv1/Alr


    UniProtKB/Swiss-Prot: QSOX1_HUMAN, O00391
    Similarity: Belongs to the quiescin-sulfhydryl oxidase (QSOX) family
    Similarity: Contains 1 ERV/ALR sulfhydryl oxidase domain
    Similarity: Contains 1 thioredoxin domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (QSOX1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001004128

                  Applied Biosystems Silencer® siRNAs for QSOX1

                  Sigma-Aldrich siRNA and siRNA Panels for QSOX1  
                         Sigma-Aldrich shRNA for QSOX1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001004128
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001004128
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001004128 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_002826

    UniProtKB/Swiss-Prot: QSOX1_HUMAN, O00391
    Function: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides
    with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a
    variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being
    involved in growth regulation
    Catalytic activity: 4 R'C(R)SH + O(2) = 2 R'C(R)S-S(R)CR' + 2 H(2)O
    Induction: Induced in quiescent cells just as fibroblasts begin to leave the proliferative cycle
    and enter quiescence
    Enzyme Number (IUBMB): EC 1.8.3.2 

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016491 oxidoreductase activity IEA--
    GO:0016971 flavin-linked sulfhydryl oxidase activity IGI17331072
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    2 Interacting proteins for QSOX1 (ENSP000003565743 O003912) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBQLN4Q9NRR52MINT-2862369
    TXNENSP000003636413STRING (score=.821)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006467 protein thiol-disulfide exchange IGI17331072
    GO:0045454 cell redox homeostasis IEA--
    GO:0055114 oxidation reduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for QSOX1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (QSOX1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001004128

                  Sigma-Aldrich siRNA and siRNA Panels for QSOX1  
                         Sigma-Aldrich shRNA for QSOX1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001004128  NM_002826  

    REFSEQ mRNAs for QSOX1 gene (2 alternative transcripts): 

    NM_001004128.2   NM_002826.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001004128  NM_002826  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001004128
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001004128
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001004128 

    Additional cDNA sequence: 

    AB209280.1 AF361868.1 AK074447.1 AK290842.1 AK290887.1 AL832927.1 AY358941.1 BC017692.1 
    BC100023.1 CR597598.1 CR601148.1 CR613263.1 CR619667.1 U97276.2 

    24/43 DOTS entries (see all 43 ):

    DT.100829879  DT.100059508  DT.99973359  DT.92440983  DT.102840166  DT.86855929  DT.86841789  DT.97759904 
    DT.100724595  DT.101967478  DT.99968131  DT.102840180  DT.100691414  DT.121434083  DT.100053977  DT.121434161 
    DT.102840170  DT.102840174  DT.102844889  DT.91750186  DT.102840182  DT.92440981  DT.100654003  DT.100654006 

    24/1123 AceView cDNA sequences (see all 1123 ):

    BQ920061 AI250666 BM561487 CO248497 BQ007832 CA488461 NM_002826 CO248765 
    BM773007 BQ684306 BM771903 BM773008 BQ422933 CF125105 CF132132 BU944785 
    CF132176 CA309936 CA390872 AI188675 AU077242 AI986162 CA489847 CO249430 

    Unigene Cluster for QSOX1:

    Quiescin Q6 sulfhydryl oxidase 1
    Hs.719176  [show with all ESTs]
    Unigene Representative Sequence: NM_002826


    GeneLoc Exon Structure

    5/9 Alternative Splicing Database (ASD) splice patterns (SP) for QSOX1 (see all 9 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12a · 12b ^ 13a · 13b · 13c ^ 14a · 14b
    SP1:              -                                                                                         -     -               
    SP2:              -                                                                                                               
    SP3:                          -                                                                                                   
    SP4:              -     -     -     -     -     -     -                                                                           
    SP5:        -     -                                                                                                               

    About this scheme

    ECgene alternative splicing isoforms for QSOX1

    4 Ensembl transcripts including schematic representations:
    ENST00000392031  ENST00000392029  ENST00000367600  ENST00000367602  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    QSOX1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for QSOX1

    1 / 2

    3 probe-sets matching QSOX1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    1257_s_at2 U95-A 1 1.00 1.00 1.00 1.00 -- -- -- -- --

    201482_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    201482_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  QSOX1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.719176

    UniProtKB/Swiss-Prot: QSOX1_HUMAN, O00391
    Tissue specificity: Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very
    weakly in brain and kidney

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for QSOX1 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    QSOX11   -- quiescin Q6 sulfhydryl oxidase 1 83.07(n)
    75.76(a)
    490299  XM_547419.2  XP_547419.2 
    cow
    (Bos taurus)
    QSOX11   -- quiescin Q6 sulfhydryl oxidase 1 82.41(n)
    74.08(a)
    522986  XM_601276.3  XP_601276.3 
    rat
    (Rattus norvegicus)
    Qsox11   -- quiescin Q6 sulfhydryl oxidase 1 81.15(n)
    77.56(a)
    84491  NM_053431.1  NP_445883.1 
    mouse
    (Mus musculus)
    Qsox11, 5 15
    quiescin Q6 sulfhydryl oxidase 11, 5 78.77(n)1
    73.41(a)1
    1040091  NM_001024945.11  NP_001020116.11 
     AB0442845  AK0048805  (see all 23)
    chicken
    (Gallus gallus)
    QSOX11   -- quiescin Q6 sulfhydryl oxidase 1 62.29(n)
    54.4(a)
    373914  NM_204125.1  NP_989456.1 
    About this table        Species with no ortholog for QSOX1

    ENSEMBL Gene Tree for QSOX1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for QSOX1 gene
    QSOX22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/364 NCBI SNPs in QSOX1 are shown (see all 364 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 196)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs38432731,2
    A,C,F,H178434063(-) AGGCTT/CCTCCC 2 -- ng31 trp316Minor allele frequency- C:0.26EA NA EU WA MN 1288
    rs37381121,2
    A,C,F,H178434037(-) ACTGAG/CGCAGG 2 -- ng31 ese38Minor allele frequency- C:0.46EA EU WA NA 1854
    rs75441471,2
    A,C,F,H178434267(+) TTGGTT/CTTCTC 2 -- ng31 trp311Minor allele frequency- C:0.30EU EA WA NA 980
    rs37381151,2
    C,F,H178432322(-) GGTGTT/GTGTGG 2 H/N mis1 ese311Minor allele frequency- G:0.31EA EU WA 2502
    rs38942111,2
    C,F,H178402324(-) GGATGT/CTGAAG 2 S/N mis1 ese39Minor allele frequency- C:0.02MN EU EA WA 1018
    rs178554751,2
    C,F,H178414635(+) CCTGGG/CTAGAG 2 A/G mis1 ese38Minor allele frequency- C:0.07EU EA WA 838
    --
    rs75162571,2
    A,F,H178389435(+) cacctT/Cctctg 2 -- ng514Minor allele frequency- C:0.09EU EA WA 418
    rs75971,2
    C,F,H178433713(+) GGGGGC/TAGTGC 2 -- ut31 ese313Minor allele frequency- T:0.42EA MN EU WA NA 2654
    rs123711,2
    C,F178430013(+) CGTGCA/GCTACT 2 H/R mis12Minor allele frequency- G:0.06MN 368
    --
    rs75301681,2
    A,C178389198(+) AGCACC/TACATT 2 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for QSOX1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603120

    1 Novoseek disease relationship for QSOX1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    prostate cancer 16.97 1 9878249 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/25 PubMed articles for QSOX1 gene (see all 25 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 5768 HGNC: 9756 AceView: QSCN6 Ensembl:ENSG00000116260 euGenes: HUgn5768
    ECgene: QSOX1 H-InvDB: QSOX1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for QSOX1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for QSOX1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for QSOX1 Browse small molecules at Sigma-Aldrich
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