PUS3 Gene
protein-coding GIFtS: 49
GCID: GC11M125763
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pseudouridylate synthase 3
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Aliases
for PUS3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Pseudouridylate Synthase 31 2 | | FKSG321 | | TRNA Pseudouridine Synthase 32 3 | | 2610020J05Rik2 | | TRNA Pseudouridylate Synthase 32 3 | | TRNA Pseudouridine(38/39) Synthase2 | | TRNA-Uridine Isomerase 32 3 | | EC 5.4.99.453 |
Export aliases for PUS3 gene to outside databasesPrevious GC identifers: GC11M125268 GC11M121705 |
Summaries
for PUS3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for PUS3:
The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in theanticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for thisgene. (provided by RefSeq, Dec 2012)
UniProtKB/Swiss-Prot: PUS3_HUMAN, Q9BZE2Function: Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs (By similarity)
|
Genomic Views
for PUS3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000011.9 NC_018922.1 NT_033899.8
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PUS3 gene promoter:
TBP AML1a Tal-1 Pax-6 Pax-2 Pax-2a GATA-2 E47 AREB6
Other transcription factors
Search SABiosciences Chromatin IP Primers for PUS3
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PUS3 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 11q24.2 Ensembl cytogenetic band: 11q24.2 HGNC cytogenetic band: 11q24.2PUS3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11M125763: view genomic region
(about GC identifiers)
Start:
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125,763,380 bp from pter |
End:
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125,773,116 bp from pter |
Size:
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9,737 bases |
Orientation:
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minus strand |
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Proteins
for PUS3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: PUS3_HUMAN, Q9BZE2 (See
protein sequence)Recommended Name: tRNA pseudouridine(38/39) synthase Size: 481 amino acids; 55647 Da
Subcellular location: Nucleus (Potential)
Secondary accessions: B2RAM0 Q96D17 Q96J23 Q96NB4Explore the universe of human proteins at neXtProt for PUS3: NX_Q9BZE2
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9BZE2
PUS3 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001258914.1 NP_112597.3
ENSEMBL proteins: ENSP00000432386 ENSP00000432272 ENSP00000437077 ENSP00000227474
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- |
PUS3 for ontologies About GeneDecksing
PUS3 Antibody Products:
Assay Products for PUS3: |
Protein
Domains /
Families
for PUS3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PUS3 for domains About GeneDecksing
5 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9BZE2ProtoNet protein and cluster: Q9BZE2 1 Blocks protein family: IPB001406 tRNA pseudouridine synthase
UniProtKB/Swiss-Prot: PUS3_HUMAN, Q9BZE2Similarity: Belongs to the tRNA pseudouridine synthase TruA family |
Function
for PUS3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: PUS3_HUMAN, Q9BZE2Function: Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs (By similarity)Catalytic activity: tRNA uridine(38/39) = tRNA pseudouridine(38/39)Enzyme Number (IUBMB): EC 5.4.99.451
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PUS3
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for PUS3 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PUS3 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PUS3 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003723 | RNA binding |
IEA | -- | | GO:0009982 | pseudouridine synthase activity |
IEA | -- |
PUS3 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for PUS3:
|
Pathways & Interactions
for PUS3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PUS3
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/144 Interacting proteins for PUS3 (Q9BZE23 ENSP000002274744) via UniProtKB, MINT, STRING, and/or I2D (see all 144)About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0031119 | tRNA pseudouridine synthesis |
IEA | -- |
PUS3 for ontologies About GeneDecksing
|
Drugs & Compounds
for PUS3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for PUS3
2 HMDB Compounds for PUS3 About this table
Search CenterWatch for drugs/clinical trials and news about PUS3
|
Transcripts
for PUS3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for PUS3 gene (2 alternative transcripts): NM_001271985.1 NM_031307.3 Unigene Cluster for PUS3: Pseudouridylate synthase 3 Hs.660922 [show with all ESTs]Unigene Representative Sequence: NM_0313074 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000530811 ENST00000534158 ENST00000529801 ENST00000227474(uc001qcy.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PUS3 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PUS3
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: PUS3 (NM_031307) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for PUS3 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PUS3 |
Additional cDNA sequence: AF325689.1 AK055702.1 AK074218.1 AK314251.1 BC004822.1 BC013427.2 4 DOTS entries: DT.410928 DT.100812546 DT.91887256 DT.95156177 24/57 AceView cDNA sequences (see all 57): AA376153 BG113960 BM903885 BG421546 BF972366 AA988818 CA942585 NM_031307 BM459808 BU145971 AF325689 AA432136 BQ230942 BM759156 BX095434 AA127469 BU517204 AA608701 BM845153 AK055702 BX488578 AA594661 AW016543 AA431120
GeneLoc Exon Structure
|
Expression
for PUS3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| PUS3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AGGAAGAAAC
About this image
See PUS3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for PUS3
SOURCE GeneReport for Unigene cluster: Hs.660922
SABiosciences Custom PCR Arrays for PUS3
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PUS3
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat PUS3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PUS3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PUS3 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PUS3 |
Orthologs
for PUS3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for PUS3 gene from 9/31 species (see all 31) About this table
ENSEMBL Gene Tree for PUS3 (if available)
TreeFam Gene Tree for PUS3 (if available) |
Paralogs
for PUS3 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for PUS3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 11 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for PUS3 (125763380 - 125773116 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for PUS3
1 CNV: 6905 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PUS3 |
|
Disorders
/ Diseases
for PUS3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PUS3 for disorders About GeneDecksing
3  diseases for PUS3: About MalaCardssideroblastic anemia anemia malaria
1 disease from the University of Copenhagen DISEASES database for PUS3:Sideroblastic anemia Export disorders for PUS3 gene to outside databases
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Publications
for PUS3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for PUS3 gene integrated from 9 sources:
(articles sorted by number of sources associating them with PUS3) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (2002)
- Pseudouridine synthase 3 from mouse modifies the anticodon loop of tRNA. (PubMed id 11027153)1, 9 Chen J. and Patton J.R. (2000)
- A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)2 Dephoure N.... Gygi S.P. (2008)
- Transcriptome analysis of human gastric cancer. (PubMed id 16341674)1 Oh J.H....Kim N.S. (2005)
- Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )2 Aoki H.... Hino O. (2000)
|
External Searches for PUS3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
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Genome Databases showing PUS3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing PUS3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PUS3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for PUS3 | Pharmacogenomics, SNPs, Pathways |
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| | |
About This Section
| Patent Information for PUS3 gene:
Search GeneIP for patents involving PUS3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PUS3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for PUS3 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for PUS3 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for PUS3 | | | OriGene Protein Over-expression Lysate for PUS3 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for PUS3 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for PUS3 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for PUS3 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for PUS3 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat PUS3 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing PUS3 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PUS3 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat PUS3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat PUS3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat PUS3 |
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| | | | Search Tocris compounds for PUS3 |
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 | | | PUS3 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PUS3 |
|  |  |  | | | | ThermoFisher Antibodies for PUS3 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PUS3 |
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