Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



PUM2 Gene

protein-coding   GIFtS: 64
GCID: GC02M020448

Pumilio RNA-Binding Family Member 2

(Previous names: pumilio (Drosphila) homolog 2, pumilio homolog 2 (Drosophila))
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Pumilio RNA-Binding Family Member 21 2     PUML22
PUMH22 3     Pumilio Homolog 22
KIAA02353 5     pumilio-22
Pumilio (Drosphila) Homolog 21     Pumilio-23
Pumilio Homolog 2 (Drosophila)1     

External Ids:    HGNC: 149581   Entrez Gene: 233692   Ensembl: ENSG000000559177   OMIM: 6072055   UniProtKB: Q8TB723   

Export aliases for PUM2 gene to outside databases

Previous GC identifers: GC02M020339 GC02M020516 GC02M020416 GC02M020432 GC02M020370 GC02M020311 GC02M020205


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for PUM2 Gene:
This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a
translational repressor during embryonic development and cell differentiation. This protein is also thought to be
a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple
transcript variants. (provided by RefSeq, Sep 2013)

GeneCards Summary for PUM2 Gene:
PUM2 (pumilio RNA-binding family member 2) is a protein-coding gene. Diseases associated with PUM2 include essential tremor, and tremor. GO annotations related to this gene include RNA binding. An important paralog of this gene is PUM1.

UniProtKB/Swiss-Prot: PUM2_HUMAN, Q8TB72
Function: Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by
binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE),
5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and
-independent modes of repression. Its interactions and tissue specificity suggest that it may be required to
support proliferation and self-renewal of stem cells by regulating the translation of key transcripts

Gene Wiki entry for PUM2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000002.12  NT_022184.16  NC_018913.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the PUM2 gene promoter:
         TBP   SRF   Pbx1a   SRF (504 AA)   CUTL1   HNF-3beta   Gfi-1   POU2F1   POU2F1a   FOXO4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidPUM2 promoter sequence
   Search Chromatin IP Primers for PUM2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PUM2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p22-p21   Ensembl cytogenetic band:  2p24.1   HGNC cytogenetic band: 2p22-p21

PUM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PUM2 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M020448:  view genomic region     (about GC identifiers)

Start:
20,448,452 bp from pter      End:
20,551,995 bp from pter
Size:
103,544 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: PUM2_HUMAN, Q8TB72 (See protein sequence)
Recommended Name: Pumilio homolog 2  
Size: 1066 amino acids; 114216 Da
Subunit: Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Interacts
with NANOS3 (By similarity). Interacts with SNAPIN. Recruits the CCR4-POP2-NOT deadenylase leading to
translational inhibition and mRNA degradation. Interacts with DDX20
3 PDB 3D structures from and Proteopedia for PUM2:
3Q0Q (3D)        3Q0R (3D)        3Q0S (3D)    
Secondary accessions: B3KSL0 B4E2B6 D6W527 O00234 Q53TV7 Q8WY43 Q9HAN2
Alternative splicing: 4 isoforms:  Q8TB72-1   Q8TB72-2   Q8TB72-3   Q8TB72-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for PUM2: NX_Q8TB72

Explore proteomics data for PUM2 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys672, Lys964
  • Modification sites at PhosphoSitePlus

  • See PUM2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001269681.1  NP_001269719.1  NP_001269720.1  NP_056132.1  

    ENSEMBL proteins: 
     ENSP00000338173   ENSP00000409905   ENSP00000385992   ENSP00000387399   ENSP00000396727  
     ENSP00000408384   ENSP00000413515   ENSP00000395098   ENSP00000354370   ENSP00000326746  
     ENSP00000440093  

    PUM2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for PUM2
    OriGene Protein Over-expression Lysate for PUM2
    OriGene MassSpec for PUM2
    OriGene Custom Protein Services for PUM2
    GenScript Custom Purified and Recombinant Proteins Services for PUM2
    Novus Biologicals PUM2 Protein
    Novus Biologicals PUM2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for PUM2

    PUM2 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of PUM2
    R&D Systems Antibodies for PUM2
    Cell Signaling Technology (CST) Antibodies for PUM2  (pumilio 2)
    OriGene Antibodies for PUM2
    OriGene Custom Antibody Services for PUM2
    Novus Biologicals PUM2 Antibodies
    Abcam antibodies for PUM2
    Cloud-Clone Corp. Antibodies for PUM2
    ThermoFisher Antibodies for PUM2
    LSBio Antibodies in human, mouse, rat for PUM2

    PUM2 Assay Products:

    EMD Millipore Kits and Assays for the Analysis of PUM2
    OriGene Custom Assay Services for PUM2
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for PUM2
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for PUM2
    Cloud-Clone Corp. CLIAs for PUM2


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    3 InterPro protein domains:
     IPR011989 ARM-like
     IPR001313 Pumilio_RNA-bd_rpt
     IPR016024 ARM-type_fold

    Graphical View of Domain Structure for InterPro Entry Q8TB72

    ProtoNet protein and cluster: Q8TB72

    UniProtKB/Swiss-Prot: PUM2_HUMAN, Q8TB72
    Domain: The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix
    that approximates a half doughnut. RNA-binding occurs on the concave side of the surface (PubMed:21397187)
    Similarity: Contains 1 PUM-HD domain
    Similarity: Contains 8 pumilio repeats


    PUM2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: PUM2_HUMAN, Q8TB72
    Function: Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by
    binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE),
    5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and
    -independent modes of repression. Its interactions and tissue specificity suggest that it may be required to
    support proliferation and self-renewal of stem cells by regulating the translation of key transcripts
    Induction: Down-regulated in keratinocytes upon UVB irradiation

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003723RNA binding ----
    GO:0005488binding ----
    GO:0005515protein binding IPI12511597
    GO:0044822poly(A) RNA binding IDA--
         
    PUM2 for ontologies           About GeneDecksing


    Phenotypes:
         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Pum2):
     endocrine/exocrine gland  reproductive system 

    PUM2 for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PUM2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for PUM2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for PUM2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for PUM2

    miRNA
    Products:
        
    miRTarBase miRNAs that target PUM2:
    hsa-mir-615-3p (MIRT039900), hsa-mir-10a-5p (MIRT025614), hsa-mir-125a-5p (MIRT021291), hsa-mir-34a-5p (MIRT025372), hsa-mir-218-5p (MIRT024251)

    Block miRNA regulation of human, mouse, rat PUM2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PUM2 (see all 62):
    hsa-miR-579 hsa-miR-582-3p hsa-miR-3164 hsa-miR-2052 hsa-miR-19b-2* hsa-miR-429 hsa-miR-485-3p hsa-miR-624
    SwitchGear 3'UTR luciferase reporter plasmidPUM2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for PUM2
    Predesigned siRNA for gene silencing in human, mouse, rat PUM2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for PUM2

    Clone
    Products:
         
    OriGene clones in human, mouse for PUM2 (see all 10)
    OriGene ORF clones in mouse, rat for PUM2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: PUM2 (NM_015317)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PUM2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PUM2

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for PUM2
    Browse ESI BIO Cell Lines and PureStem Progenitors for PUM2 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PUM2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    PUM2_HUMAN, Q8TB72: Cytoplasm (Probable). Cytoplasmic granule (By similarity). Cytoplasm, perinuclear region.
    Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of
    proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By
    similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol3
    nucleus2

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0010494cytoplasmic stress granule ISS--
    GO:0031965nuclear membrane IDA--
    GO:0048471perinuclear region of cytoplasm IDA19168546

    PUM2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for PUM2 About    
    See pathways by source

    SuperPathContained pathways About
    1Translational Control
    Translational Control

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for PUM2
        Translational Control



    PUM2 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Array including PUM2: 
              Cardiotoxicity in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for PUM2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for PUM2 (Q8TB721, 2, 3 ENSP000003381734) via UniProtKB, MINT, STRING, and/or I2D (see all 759)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DAZ1Q9NQZ31, 2, 3, ENSP000003845734EBI-311190,EBI-997955 MINT-61576 MINT-45963 MINT-61575 I2D: score=3 STRING: ENSP00000384573
    AURKAO149652, 3, ENSP000002169114MINT-7945693 I2D: score=1 STRING: ENSP00000216911
    DAZ3Q9NR903, ENSP000003718024I2D: score=1 STRING: ENSP00000371802
    DAZLQ929043, ENSP000003823734I2D: score=1 STRING: ENSP00000382373
    NANOS1Q8WY413, ENSP000003932754I2D: score=3 STRING: ENSP00000393275
    About this table

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006417regulation of translation IEA--
    GO:0034063stress granule assembly IEA--

    PUM2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for PUM2



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for PUM2 gene (4 alternative transcripts): 
    NM_001282752.1  NM_001282790.1  NM_001282791.1  NM_015317.2  

    Unigene Cluster for PUM2:

    Pumilio homolog 2 (Drosophila)
    Hs.467824  [show with all ESTs]
    Unigene Representative Sequence: AF315591
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000338086(uc002rds.1 uc002rdt.1 uc002rdu.1 uc010yjz.1)
    ENST00000440577 ENST00000403432 ENST00000442400 ENST00000420234 ENST00000429419
    ENST00000432105 ENST00000446940 ENST00000424110 ENST00000361078(uc002rdq.1)
    ENST00000319801(uc002rdr.2 uc010yjy.1) ENST00000536417
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat PUM2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate PUM2 (see all 62):
    hsa-miR-579 hsa-miR-582-3p hsa-miR-3164 hsa-miR-2052 hsa-miR-19b-2* hsa-miR-429 hsa-miR-485-3p hsa-miR-624
    SwitchGear 3'UTR luciferase reporter plasmidPUM2 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for PUM2
    Predesigned siRNA for gene silencing in human, mouse, rat PUM2
    Clone
    Products:
         
    OriGene clones in human, mouse for PUM2 (see all 10)
    OriGene ORF clones in mouse, rat for PUM2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: PUM2 (NM_015317)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for PUM2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PUM2
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for PUM2
    OriGene qSTAR qPCR primer pairs in human, mouse for PUM2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat PUM2
      QuantiTect SYBR Green Assays in human, mouse, rat PUM2
      QuantiFast Probe-based Assays in human, mouse, rat PUM2

    Additional mRNA sequence: 

    AF272350.1 AF315591.1 AK093847.1 AK304198.1 BC024218.2 BC112046.1 BC112048.1 BC143550.1 
    BC143552.1 BX641165.1 D87078.3 

    22 DOTS entries:

    DT.454374  DT.97777829  DT.95202438  DT.99938620  DT.87078922  DT.439165  DT.95202445  DT.100795116 
    DT.121031120  DT.40210334  DT.91698460  DT.95202442  DT.100795118  DT.121031128  DT.121031166  DT.121031194 
    DT.121031198  DT.75148452  DT.75158918  DT.92443916  DT.92443918  DT.97798173 

    Selected AceView cDNA sequences (see all 302):

    BG779152 CN484734 AI588883 AI521020 BX956828 AF272350 BG682050 BM785354 
    AA020786 BP367941 AW661965 AA918513 AA496663 AI129257 CA390424 R17841 
    BM769755 D87078 AV682869 BQ212274 BX645799 AI915292 AA461053 AA282601 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for PUM2 (see all 9)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15a · 15b ^ 16 ^ 17a · 17b ^ 18a · 18b ^ 19a ·
    SP1:              -     -                                                                       -                                               -               
    SP2:                                                                                                                                            -               
    SP3:        -     -     -                                                                                                                                       
    SP4:                                                                                                                                                            
    SP5:                                                                                      -     -     -                                                         

    ExUns: 19b ^ 20a · 20b ^ 21 ^ 22 ^ 23 ^ 24 ^ 25a · 25b
    SP1:                                                      
    SP2:                                                      
    SP3:                                                      
    SP4:                                                      
    SP5:                                                      


    ECgene alternative splicing isoforms for PUM2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    PUM2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GAGGCCAATG
    PUM2 Expression
    About this image


    PUM2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Inner Cell Mass (Early Embryonic Tissues)    fully expand to see all 3 entries
             Human embryonic stem cells (family)
     
     Limb (Muscoskeletal System)
             Limb Bud
     
     Thyroid (Endocrine System)
    PUM2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    PUM2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.467824

    UniProtKB/Swiss-Prot: PUM2_HUMAN, Q8TB72
    Tissue specificity: Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis
    and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart,
    kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus,
    hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested

        Pathway & Disease-focused RT2 Profiler PCR Array including PUM2: 
              Cardiotoxicity in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for PUM2
    OriGene qSTAR qPCR primer pairs in human, mouse for PUM2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat PUM2
    QuantiTect SYBR Green Assays in human, mouse, rat PUM2
    QuantiFast Probe-based Assays in human, mouse, rat PUM2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PUM2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for PUM2 gene from Selected species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Pum21 , 5 pumilio 2 (Drosophila)1, 5 94.31(n)1
    97.74(a)1
      12 (3.92 cM)5
    809131  NM_030723.21  NP_109648.21 
     86741345 
    chicken
    (Gallus gallus)
    Aves PUM21 pumilio homolog 2 (Drosophila) 86.57(n)
    93.77(a)
      421961  NM_001039292.1  NP_001034381.1 
    lizard
    (Anolis carolinensis)
    Reptilia PUM26
    pumilio RNA-binding family member 2
    93(a)
    1 ↔ 1
    1(155114854-155172944)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia pum21 pumilio homolog 2 (Drosophila) 76.94(n)
    84.73(a)
      100496617  XM_002934483.2  XP_002934529.2 
    zebrafish
    (Danio rerio)
    Actinopterygii pum26
    pumilio homolog 2 (Drosophila)
    68(a)
    1 ↔ 1
    20(6140108-6182554) ENSDARG00000061040
    fruit fly
    (Drosophila melanogaster)
    Insecta pum3 head involution protein binding 79(a)     --
    worm
    (Caenorhabditis elegans)
    Secernentea puf-93 pumilo repeats 59(a)
    (best of 2)
      X(5876886-5880071)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes PUF3(YLL013C)4 Protein of the mitochondrial outer surface, links the more   --   12(124714-122075) 850647  NP_013088.1 
    soybean
    (Glycine max)
    eudicotyledons Gma.24662 Transcribed sequence with moderate similarity to protein more 80.56(n)  
    rice
    (Oryza sativa)
    Liliopsida AK070870.12   -- 77.66(n)    AK070870.1 


    ENSEMBL Gene Tree for PUM2 (if available)
    TreeFam Gene Tree for PUM2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for PUM2 gene
    PUM12  
    1 SIMAP similar gene for PUM2 using alignment to 6 protein entries:     PUM2_HUMAN (see all proteins):
    PUM1

    PUM2 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for PUM2
    PGOHUM00000236316


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for PUM2 (see all 1642)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs798150211,2
    --20448188(+) CAAGGC/TGTGTA 1 -- ds50010--------
    rs1817805291,2
    --20448250(+) CAACAA/CTGCCT 1 -- ds50010--------
    rs1487854561,2
    --20448276(+) AAAACA/GGGCTA 1 -- ds50010--------
    rs1439499441,2
    --20448292(+) TTTATC/TCTATG 1 -- ds50010--------
    rs1467845431,2
    --20448370(+) GCTCCA/GGAACA 1 -- ds50010--------
    rs1147299591,2
    F--20448379(+) CAGCAC/TCCATG 1 -- ds50011Minor allele frequency- T:0.01WA 118
    rs1841385841,2
    --20448413(+) ACGTCA/GGAGTA 1 -- ds50010--------
    rs66691,2
    C,F--20448521(-) AATGTG/ATGAAT 1 -- ut313Minor allele frequency- A:0.04MN NA 306
    rs1395523561,2
    --20448538(+) TTTCTA/GATTTA 1 -- ut310--------
    rs10434191,2
    C,F,H--20448561(-) TTCACT/CTCTTT 1 -- ut31 ese319Minor allele frequency- C:0.11MN NS EA NA 2420

    HapMap Linkage Disequilibrium report for PUM2 (20448452 - 20551995 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for PUM2:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv29979CNV Loss17803354
    nsv524207CNV Gain19592680

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing PUM2
    DNA2.0 Custom Variant and Variant Library Synthesis for PUM2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 607205    OMIM disorders: --

    5 diseases for PUM2:    
    About MalaCards
    essential tremor    tremor    azoospermia    squamous cell carcinoma
    malaria

    1 disease from the University of Copenhagen DISEASES database for PUM2:
    Essential tremor

    PUM2 for disorders           About GeneDecksing

    Genetic Association Database (GAD): PUM2
    Human Genome Epidemiology (HuGE) Navigator: PUM2 (1 document)

    Export disorders for PUM2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for PUM2 gene, integrated from 10 sources (see all 43):
    (articles sorted by number of sources associating them with PUM2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-like proteins. (PubMed id 12511597)1, 2, 3 Moore F.L.... Reijo Pera R.A. (Proc. Natl. Acad. Sci. U.S.A. 2003)
    2. Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins. (PubMed id 12459267)1, 2, 3 Spassov D.S. and Jurecic R. (Gene 2002)
    3. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. (PubMed id 9039502)1, 2, 3 Nagase T.... Nomura N. (DNA Res. 1996)
    4. The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells. (PubMed id 19168546)1, 2, 9 Ginter-Matuszewska B.... Jaruzelska J. (Mol. Hum. Reprod. 2009)
    5. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. (PubMed id 12690449)1, 2, 9 Jaruzelska J....Reijo Pera R.A. (Dev. Genes Evol. 2003)
    6. Alternate modes of cognate RNA recognition by human PUMILIO proteins. (PubMed id 21397187)1, 2 Lu G. and Hall T.M. (Structure 2011)
    7. NANOS1 and PUMILIO2 bind microRNA biogenesis factor GEMIN3, within chromatoid body in human germ cells. (PubMed id 21800163)1, 2 Ginter-Matuszewska B....Jaruzelska J. (Histochem. Cell Biol. 2011)
    8. Polymorphisms of the human PUMILIO2 gene and male sterility. (PubMed id 17154300)1, 4 Kusz K....Jaruzelska J. (Mol. Reprod. Dev. 2007)
    9. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W.... Wilson R.K. (Nature 2005)
    10. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 23369 HGNC: 14958 AceView: PUM2 Ensembl:ENSG00000055917 euGenes: HUgn23369
    ECgene: PUM2 H-InvDB: PUM2

    (According to HUGE)
    About This Section

    TryGeneCards Plus
    HUGE: KIAA0235

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for PUM2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for PUM2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for PUM2 gene:
    Search GeneIP for patents involving PUM2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     Browse Small Molecules at EMD Millipore
     Browse Purified and Recombinant Proteins at EMD Millipore
     EMD Millipore Kits and Assays for the Analysis of PUM2
     EMD Millipore Mono- and Polyclonal Antibodies for the study of PUM2
     
     EMD Millipore genomic analysis products

      
     Antibodies for PUM2 (PUM2)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for PUM2   OriGene RNAi products in human, mouse, rat for PUM2  
     OriGene qPCR primer pairs and template standards for PUM2   OriGene Protein Over-expression Lysate for PUM2  
     OriGene MassSpec something-or-other for PUM2   OriGene clones in human, mouse for PUM2  
     OriGene qSTAR qPCR primer pairs in human, mouse for PUM2   OriGene Purified Protein for PUM2  
     OriGene ORF clones in mouse, rat for PUM2   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for PUM2   OriGene Custom Protein Services for PUM2  

     
     
     Block miRNA regulation of human, mouse, rat PUM2 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing PUM2
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat PUM2 Predesigned siRNA for gene silencing in human, mouse, rat PUM2
     QuantiFast Probe-based Assays in human, mouse, rat PUM2 QuantiTect SYBR Green Assays in human, mouse, rat PUM2
     PCR Arrays including human, mouse, rat PUM2 Search Chromatin IP Primers for PUM2
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat PUM2  GeneGlobe Interaction Network for PUM2
     Regulatory tfbs in PUM2 promoter
     GenScript Custom Purified and Recombinant Proteins Services for PUM2 GenScript cDNA clones with any tag delivered in your preferred vector for PUM2
     GenScript Custom Assay Services for PUM2 GenScript Custom overexpressing Cell Line Services for PUM2
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for PUM2 
    (pumilio 2)

     Search Tocris compounds for PUM2
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     PUM2 antibodies
     PUM2 proteins
     PUM2 lysates
     Antibodies for PUM2
     See all of Abcam's Antibodies, Kits and Proteins for PUM2
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for PUM2
     Antibodies for PUM2
     ELISAs for PUM2
     CLIAs for PUM2



     Browse ESI BIO Cell Lines and PureStem Progenitors for PUM2
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for PUM2
     SwitchGear 3'UTR luciferase reporter plasmids for PUM2
     SwitchGear Promoter luciferase reporter plasmids for PUM2
     ThermoFisher Antibodies for PUM2
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat PUM2
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for PUM2
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for PUM2
     LSBio Antibodies in human, mouse, rat for PUM2
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      PUM2 gene at Home site.
    Version: 3.12.164 25 Aug 2014
    hostname: 356977-web1.xennexinc.com index build: 126 solr: 1.4