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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

PTTG1 Gene

protein-coding GIFtS: 60
GCID: GC05P159781

pituitary tumor-transforming 1

(Previous symbol: TUTR1)

Explore 56 diseases affiliated with
PTTG1 via

MalaCards

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Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Pituitary Tumor-Transforming 1¹ ²		Pituitary Tumor-Transforming Gene 1 Protein² ³
EAP1¹ ² ³ ⁵		Tumor-Transforming Protein 1² ³
TUTR1¹ ² ³ ⁵		ESP1-Associated Protein 1²
PTTG¹ ² ³		Esp1-Associated Protein³
HPTTG¹ ²		HPTTG¹ ²
Securin¹

External Ids:	HGNC: 9690¹	Entrez Gene: 9232²	Ensembl: ENSG00000164611⁷	OMIM: 604147⁵	UniProtKB: O95997³

Export aliases for PTTG1 gene to outside databases

Previous GC identifers: GC05P159967 GC05P160358 GC05P159784 GC05P159829 GC05P154942

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for PTTG1:

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid

separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the

APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly

expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and

tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains

a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded

protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially

localizes in the nucleus. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: PTTG1_HUMAN, O95997

Function: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA

repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function,

preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of

anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however

not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional

activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming

capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku,

possibly by connecting DNA damage-response pathways with sister chromatid separation

Gene Wiki entry for PTTG1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000005.9 NC_018916.1 NT_023133.13

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the PTTG1 gene promoter:
Nkx3-1 v4 Elk-1 PPAR-gamma1 Sp1 Lmo2 PPAR-gamma2 LCR-F1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): PTTG1 promoter sequence

Search SABiosciences Chromatin IP Primers for PTTG1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat PTTG1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q35.1 Ensembl cytogenetic band: 5q33.3 HGNC cytogenetic band: 5q35.1

PTTG1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PTTG1 gene location

GeneLoc information about chromosome 5 GeneLoc Exon Structure

GeneLoc location for GC05P159781: view genomic region (about GC identifiers)

Start:	159,848,829 bp from pter	End:	159,855,748 bp from pter
Size:	6,920 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: PTTG1_HUMAN, O95997 (See protein sequence)

Recommended Name: Securin

Size: 202 amino acids; 22024 Da

Subunit: Interacts with RPS10 and DNAJA1 (By similarity). Interacts with the caspase-like ESPL1, and prevents its

protease activity probably by covering its active site. Interacts with TP53 and blocks its activity probably by

blocking its binding to DNA. Interacts with the Ku 70 kDa subunit of ds-DNA kinase. Interacts with PTTG1IP

Subcellular location: Cytoplasm. Nucleus

Developmental stage: Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded

Explore the universe of human proteins at neXtProt for PTTG1: NX_O95997

Post-translational modifications:

Phosphorylated at Ser-165 by CDK1 during mitosis¹

Phosphorylated in vitro by ds-DNA kinase¹

Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of

anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_O95997

PTTG1 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_004210.1

ENSEMBL proteins:

ENSP00000344936 ENSP00000431068 ENSP00000430642 ENSP00000377536

Reactome Protein details: O95997
Human Recombinant Protein Products for PTTG1:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: PTTG1 OriGene Protein Over-expression Lysate: PTTG1 OriGene Custom Protein Services for PTTG1
	GenScript Custom Purified and Recombinant Proteins Services for PTTG1
	Novus Biologicals PTTG1 Proteins Novus Biologicals PTTG1 Lysate
	Browse Sino Biological Recombinant Proteins
	ProSpec Recombinant Protein for PTTG1
	Uscn Proteins for PTTG1

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005737	cytoplasm	IDA	--
GO:0005829	cytosol	TAS	--

PTTG1 for ontologies About GeneDecksing

PTTG1 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	OriGene Antibodies: PTTG1 OriGene Custom Antibody Services for PTTG1
	GenScript Custom Superior Antibodies Services for PTTG1
	Novus Biologicals PTTG1 Antibodies
	Abcam antibodies for PTTG1
	Uscn Antibodies for PTTG1
	ThermoFisher Antibodies for PTTG1

Assay Products for PTTG1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for PTTG1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for PTTG1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

PTTG1 for domains About GeneDecksing

2 InterPro domains/families:

IPR018008 Securin_separation-inh_met

IPR006940 Securin_separation_inhibitor

Graphical View of Domain Structure for InterPro Entry O95997

ProtoNet protein and cluster: O95997

UniProtKB/Swiss-Prot: PTTG1_HUMAN, O95997

Domain: The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the

ubiquitin-proteasome pathway (By similarity)

Domain: The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first

ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the

UBE2C/UBCH10-ubiquitin thioester with the acceptor lysine residue

Similarity: Belongs to the securin family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: PTTG1_HUMAN, O95997